Presence and integration of HBV DNA in mouse oocytes

doi:10.3748/wjg.v11.i19.2869

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May 21, 2005 (publication date) through Nov 12, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

Viral Hepatitis

World J Gastroenterol. May 21, 2005; 11(19): 2869-2873
Published online May 21, 2005. doi: 10.3748/wjg.v11.i19.2869

Presence and integration of HBV DNA in mouse oocytes

Tian-Hua Huang, Qing-Jian Zhang, Qing-Dong Xie, Li-Ping Zeng, Xi-Fan Zeng

Tian-Hua Huang, Qing-Jian Zhang, Qing-Dong Xie, Xi-Fan Zeng, Research Center of Reproductive Medicine, Shantou University Medical College, Shantou 515041, Guangdong Province, China

Li-Ping Zeng, Department of Gynecology and Obstetrics of the First Affiliated Hospital, Shantou University Medical College, Shantou 515041, Guangdong Province, China

Author contributions: All authors contributed equally to the work.

Correspondence to: Professor Tian-Hua Huang, Research Center of Reproductive Medicine, Shantou University Medical College, 22 Xinling Road, Shantou 515041, Guangdong Province, China. thhuang@stu.edu.cn

Telephone: +86-754-8900845 Fax: +86-754-8900845

Received: February 27, 2004
Revised: February 28, 2004
Accepted: April 20, 2004
Published online: May 21, 2005

Abstract

AIM: Hepatitis B is a worldwide public health problem. To explore the feasibility of hepatitis B virus (HBV) vertical transmission via oocytes, the presence and integration of HBV DNA in mouse oocytes were studied.

METHODS: Genomic DNA was isolated and metaphases were prepared, respectively from mouse oocytes cocultured with pBR322-HBV DNA plasmids. PCR, Southern blot, dot hybridization and fluorescence in situ hybridization (FISH) were performed to explore the existence and integration of HBV DNA in oocytes.

RESULTS: PCR detected positive bands in the tested samples, and then Southern blot revealed clear hybridization signals in PCR products. Final washing solutions were collected for dot hybridization and no signal for HBV DNA was observed, which excluded the possibility that contamination of washing solutions gave rise to positive results of PCR and Southern blot. FISH demonstrated that 36 of 1000 metaphases presented positive signals.

CONCLUSION: HBV DNA sequences are able to pass through the zona and oolemma to enter into oocytes and to integrate into their chromosomes. HBV DNA sequences might be brought into embryo via oocytes as vectors when they are fertilized with normal spermatozoa.

Keywords: HBV DNA; Transmission; Mouse oocyte; Integration; Chromosomes