Basic Research
Copyright ©The Author(s) 2004. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Mar 15, 2004; 10(6): 860-863
Published online Mar 15, 2004. doi: 10.3748/wjg.v10.i6.860
Effects of daidzein on estrogen-receptor-positive and negative pancreatic cancer cells in vitro
Jun-Ming Guo, Bing-Xiu Xiao, De-Jian Dai, Qiong Liu, Hong-Hui Ma
Jun-Ming Guo, Bing-Xiu Xiao, Qiong Liu, School of Medicine, Ningbo University, Ningbo 315211, Zhejiang Province, China
De-Jian Dai, Second Affiliated Hospital of Medical College, Zhejiang University, Huangzhou 310009, Zhejiang Province, China
Hong-Hui Ma, School of Life Science and Bioengineering, Ningbo University, Ningbo 315211, Zhejiang Province, China
Author contributions: All authors contributed equally to the work.
Supported by Scientific Research Fund of Zhejiang Provincial Education Department, No.20010217
Correspondence to: Dr. Jun-Ming Guo, School of Medicine, Ningbo University, Ningbo 315211, Zhejiang Province, China. junmingguo@yahoo.com
Telephone: +86-574-87600758 Fax: +86-574-87608638
Received: August 26, 2003
Revised: September 2, 2003
Accepted: September 18, 2003
Published online: March 15, 2004
Abstract

AIM: To study the effects of daidzein on human pancreatic cancer cells in vitro.

METHODS: Human estrogen-receptor (ER)-positive pancreatic cancer cells MiaPaCa-2 and ER-negative pancreatic cancer cells PANC-1 were treated by 0.1 μmol/L, 1 μmol/L, 10 μmol/L, 25 μmol/L, 50 μmol/L, 75 μmol/L and 100 μmol/L of daidzein, respectively. Its antiproliferative effect was studied by MTT assay.

RESULTS: Daidzein inhibited the growth of MiaPaCa-2 and PANC-1 cells at the concentrations from 0.1 μmol/L to 100 μmol/L. A dose- and time-dependent manner was found. The IC50 of daidzein on MiaPaCa-2 and PANC-1 cells was 45 μmol/L and 75 μmol/L, respectively. After MiaPaCa-2 cells were treated by daidzein for 3 d and at the concentrations more than IC50, the inhibitory manner was identical and the inhibition appeared a saturation phenomenon, but the inhibitory manner of daidzein on PANC-1 cells was different from that of MiaPaCa-2 cells.

CONCLUSION: Daidzein has antiproliferative effects on human estrogen-receptor-positive and negative pancreatic cancer cells, but their mechanisms may be different.

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