Relationship between mismatch repair protein, RAS, BRAF, PIK3CA gene expression and clinicopathological characteristics in elderly colorectal cancer patients

Table 1 Polymerase chain reaction steps

Polymerase chain reaction steps
First stage	95℃, 5 min	1 cycle
Second stage	95℃, 25 s	15 cycles
	64℃, 20 s
	72℃, 20 s
Third stage	93℃, 25 s	31 cycles
	60℃, 35 s
	72℃, 20 s

Table 2 Relationship between expression of mismatch repair protein and clinicopathological characteristics in elderly colorectal cancer patients

Clinical pathology data	n	MMR		χ2	P value
		dMMR	pMMR
Gender				0.005	0.945
Male	196	19	177
Female	131	13	118
Tumor size				3.907	0.048
≥ 5 cm	112	16	96
< 5 cm	215	16	199
Pathological type				0.456	0.796
Adenocarcinoma	281	27	254
Mucinous adenocarcinoma	43	5	38
Other	3	0	3
Tumor morphology				1.768	0.622
Ulcer type	181	19	162
Raised type	77	9	68
Ulcer raised type	45	3	42
Other	24	1	23
Tumor site				32.368	0.000
Right colon	78	19	59
Left colon	90	11	79
Rectum	159	2	157
Differentiation				8.181	0.085
High	4	1	3
High-moderate	15	1	14
Moderate	241	18	223
Moderate-poor	59	10	49
Poor	8	2	6
Lymph node metastasis				2.003	0.157
Yes	130	9	121
No	197	23	174

MMR: Mismatch repair.

Table 3 Relationship between KRAS, NRAS, BRAF, PIK3CA gene expression and clinicopathological characteristics in elderly colorectal cancer patients

Clinical pathology data	n	KRAS		χ2	P	NRAS		χ2	P	BRAF		χ2	P	PIK3CA		χ2	P value
		Wild	Mutant			Wild	Mutant			Wild	Mutant			Wild	Mutant
Gender
Male	196	108	88	0.001	0.98	192	4	0.046	0.829	189	7	0	1	191	5	0	1
Female	131	72	59			127	4			127	4			127	4
Tumor size
≥ 5 cm	112	70	42	3.825	0.051	109	3	0	1	106	6	1.254	0.263	110	2	0.124	0.725
< 5 cm	215	110	105			210	5			210	5			218	7
Pathological type
Adenocarcinoma	281	157	124	0.908	0.635	273	8	1.338	0.512	273	8	8.712	0.013	274	7	0.734	0.693
Mucinous adenocarcinoma	43	22	21			43	0			41	2			41	2
Other	3	1	2			3	0			2	1			3	0
Morphology
Ulcer	181	89	92	14.972	0.002	177	4	1.328	0.723	177	4	3.279	0.351	176	5	1.1	0.777
Raised	77	57	20			74	3			72	5			74	3
Ulcer-raised	45	21	24			44	1			44	1			44	1
Other	24	13	11			24	0			23	1			24	0
Site
Right colon	78	38	40	3.184	0.203	77	1	0.784	0.676	71	7	11.004	0.004	75	3	5.652	0.059
Left colon	90	56	34			88	2			90	0			85	5
Rectum	159	86	73			154	5			155	4			158	1
Differentiation
High	4	2	2	1.791	0.774	4	0	5.926	0.205	3	1	18.408	0.001	4	0	4.738	0.315
High-moderate	15	8	7			15	0			15	0			15	0
Moderate	241	134	107			237	4			234	7			236	5
Moderate-poor	59	30	29			55	4			58	1			55	4
Poor	8	6	2			8	0			6	2			8	0
Lymph
Yes	130	71	59	0.016	0.899	124	6	2.879	0.09	124	6	0.499	0.48	127	3	0.003	0.957
No	197	109	88			195	2			192	5			191	6

Table 4 Relationship between mismatch repair protein and KRAS, NRAS, BRAF, PIK3CA genes in elderly colorectal cancer patients

Gene name	n	MMR		χ2	P value
		dMMR	pMMR
KRAS				4.06	0.044
Wild	180	23	157
Mutant	147	9	138
NRAS				0.116	0.733
Wild	319	32	287
Mutant	8	0	8
BRAF				12.489	0.000
Wild	316	27	289
Mutant	11	5	6
PIK3CA				8.879	0.003
Wild	318	28	290
Mutant	9	4	5

MMR: Mismatch repair.