First Italian outbreak of VIM-producing Serratia marcescens in an adult polyvalent intensive care unit, August-October 2018: A case report and literature review

Table 1 Antibiotic susceptibilities, in accordance with the European Committee on Antimicrobial Susceptibility Testing of VIM-producing Serratia marcescens isolates with the date and first site of identification

	MIC (μg/mL)
AMK	≤ 4	8	≤ 4	≤ 4
AMC	> 32/2	> 32/2	> 32/2	> 32/2
AMP	> 8	> 8	> 8	> 8
FEP	> 8	> 8	> 8	> 8
CTX	> 4	> 4	> 4	> 4
CAZ	> 8	> 8	> 8	> 8
CIP	1	> 1	0.5	0.5
CST	> 4	> 4	≤ 1	≤ 1
ETP	> 1	> 1	> 1	> 1
FOF	≤ 32	64	≤ 32	≤ 32
GEN	> 4	> 4	4	4
IPM	> 8	> 8	> 8	> 8
LVX	2	> 2	1	≤ 0.5
MEM	> 8	> 8	> 8	8
PIP	> 16	> 16	> 16	> 16
TZP	> 16/4	> 16/4	> 16/4	> 16/4
TGC	> 2	> 2	> 2	> 2
TOB	> 4	> 4	> 4	> 4
SXT	> 4/76	> 4/76	> 4/76	> 4/76
Date	Aug, 1	Aug, 17	Sep, 20	Sep, 24
Site	Blood	Blood	RS	RT

AMC: Amoxicillin-clavulanic acid; AMK: Amikacin; AMP: Ampicillin; CAZ: Ceftazidime; CIP: Ciprofloxacin; CST: Colistin; CTX: Cefotaxime; ETP: Ertapenem; FEP: Cefepime; FOF: Fosfomycin; GEN: Gentamicin; IPM: Imipenem; LVX: Levofloxacin; MEM: Meropenem; PIP: Piperacillin; RS: Rectal swab; RT: Respiratory tract; SXT: Trimethoprim-sulfamethoxazole; TGC: Tigecycline; TOB: Tobramycin; TZP: Piperacillin-tazobactam.

Table 2 Clinical and epidemiological data of patients

Patient	Admission from	Age(yr)	Sex	Underlying disease(s)	Previous AT	Admission diagnosis	Date of admission	Stool screening	1° site of identifi-cation
1	Community	49	M	SC	No	DNM	May, 28	Yes	Blood
1	ICU	49	M	SC	Yes	DNM	May, 28	Yes	Blood
2	Community	69	F	rUTI	Yes	SS	Sep, 9	Yes	RS
3	Internal ward	67	F	PPD, DM, UC, SHT, HCM	Yes	HS	Sep, 17	Yes	RT
Patient	Infection (1° site)	Date of 1° isolation	2° site of identification	Infection (2° site)	Date of the 2° isolation site	Initial AT	Final AT	AT duration(d)	Outcome
1	Yes	Aug, 1	RS	No	Aug, 4	CZA + GEN	CZA + GEN	14	Favourable
1	Yes	Aug, 17	-	-	-	AMK + FOF	AMK + MEM	47	Favourable
2	No	Sep, 20	RT	Yes	Sep, 20	C/T + MTZ	C/T + MTZ + CPT	14	Death
3	No	Sep, 24	Urine	No	Sep, 28	AFG + TGC + TZP	CST + SAM + CZA + AMK + AFG	16	Death

AFG: Anidulafungin; AMK: Amikacin; AT: Antibiotic treatment; CPT: Ceftaroline; CST: Colistin; C/T: Ceftolozane-tazobactam; CZA: Ceftazidime-avibactam; DM: Diabetes mellitus; DNM: Descending necrotizing mediastinitis; FOF: Fosfomycin; GEN: Gentamicin; HCM: Hypertrophic cardiomyopathy; HS: Hypovolemic hemorrhagic shock; ICU: Intensive care unit; MEM: Meropenem; MTZ: Metronidazole; PPD: Paranoid personality disorder; RS: Rectal swab; RT: Respiratory tract; SAM: Ampicillin-sulbactam; SC: Schizophrenia; SHT: Hypothyroidism; SS: Septic shock; TGC: Tigecycline; TZP: Piperacillin-tazobactam; UC: Ulcerative Colitis; rUTI: Recurrent urinary tract infection.

Table 3 Previous reported hospital outbreaks around the world of VIM-producing Gram-negative pathogens

Year	City, Country, time span	Pathogen	Type of Hospital	Setting	VIM cases	Comments
2000	Verona, Italy; February 1997 - February 1998[29]	Pseudomonas aeruginosa	University Hospital	ICU patients	83	All patients from ICU
2000	Thessaloniki, Greece; 1996-1998[30]	Pseudomonas aeruginosa	University Hospital	ICU patients	211	More than one sample for patient;
2001	Southern Taiwan; January 1999 - December 2000[31]	Klebsiella pneumoniae	University Medical Center	ICU and Other Wards	5	Multidrug-resistant Klebsiella pneumoniae
2004	Heraklion, Greece; Summer 2001[32]	Escherichia coli	University Hospital	ICU patients	4	All patients from ICU
2004	Cali, Colombia; February 1999 - July 2003[33]	Pseudomonas aeruginosa	Tertiary Care Medical Center	ICU patients	66	All patients from ICU
2005	Larissa and Thessaloniki, Greece; December 2004 - March 2005[34]	Klebsiella pneumoniae	University Hospital	ICU and Other Wards	27	Outbreaks in distinct regions due to a single Klebsiella pneumoniae clone
2005	Calgary, Canada; May 2002 - April 2004[35]	Pseudomonas aeruginosa	1 pediatric and 3 large adult hospitals	ICU and Other Wards	228	Population-based epidemiological study of infections
2005	USA; May 2013[36]	Pseudomonas aeruginosa	Public Teaching Hospital	ICU and Other Wards	17	First outbreak of carbapenemase in USA
2005	Porto Alegre, southern Brazil; January - October 2004[37]	Pseudomonas aeruginosa	Tertiary-care Teaching Hospital	ICU and Other Wards	135	Outbreak of carbapenem-resistant
2006	Athens, Greece; March 2002-October 2002[38]	Acinetobacter baumannii	Tertiary Care Hospital	ICU and Other Wards	15	Outbreak of multiple clones of imipenem-resistant
2006	Paris, France; 2003-2004[39]	Klebsiella pneumoniae	Teaching Hospital	ICU and Other Wards	8	Recovered from clinical specimens or rectal swabs - Surgical ward or ICU patients
2006	Trieste, Italy; 1996-1997/ 2000-2002[40]	Pseudomonas aeruginosa	University Hospital	ICU and Other Wards	91	Nosocomial setting of high-level endemicity
2006	Hungary; October 2003-November 2005[41]	Pseudomonas aeruginosa	seven hospitals in Hungary	ICU and Other Wards	19	Molecular epidemiology of VIM-4 Pseudomonas sp
2007	Madrid, Spain; March 2005 - September 2006[42]	Enterobacteriaceae	University Hospital	ICU and Other Wards	25	(52% of patients were in ICU)
2007	Warsaw, Poland ; September 2003 - May2004/July 2005-January2006[43]	Pseudomonas aeruginosa	Tertiary Care Hospital	ICU and Other Wards	41	Outbreak of Pseudomonas aeruginosa infections
2007	Athens, Greece; 14 September -3 October 2005[44]	Pseudomonas aeruginosa	University Hospital	ICU and Other Wards	5	Ventilator-Associated Pneumonia (VAP)
2008	Serres, Greece; April 2005 - March 2007[45]	Acinetobacter baumanni	General Hospital	ICU patients	31	All patients from ICU
2008	Piraeus, Greece; 2005-2006[46]	Acinetobacter baumannii	General Hospital	ICU and Other Wards	6	4 ICU patients
2008	Genoa, Italy; September 2004 - March 2005[47]	Klebsiella pneumoniae	Tertiary Care Hospital	ICU and Other Wards	9	Bloodstream infections
2008	Athens, Greece; February 2004 - March 2006[48]	Klebsiella pneumoniae	three hospitals in Athens	ICU and Other Wards	67	77% ICU patients
2008	Thessaloniki, Greece; November 2006 - April 2007[49]	Klebsiella pneumoniae	Tertiary Care Hospital	Wards	9	Patients hospitalized in different medical and surgical wards
2008	Nantes, France; April 1996 - July 2004[50]	Pseudomonas aeruginosa	University Hospital	ICU and Other Wards	59	Mostly urinary tract infections and pneumonia
2008	UK; November 2003-November 2007[51]	Pseudomonas aeruginosa	12 UK Hospital	ICU patients	32	15 cases from same hospital
2009	Greece; February 2008 - December 2008[52]	Klebsiella pneumoniae	21 Greek hospitals	ICU patients	52	All patients from ICU
2009	Thessaloniki, Greece; November 2004 - December 2005[53]	Pseudomonas aeruginosa	University Hospital	ICU patients	29	All patients from ICU
2010	Zonguldak, Turkey; 2003–2006[54]	Acinetobacter baumannii	University Hospital	ICU and Other Wards	116	Tracheal aspirates (32%), wound swabs (22%), blood (14%), bronchoalveolar specimens (11%) and urine, sterile fluids, catheter tips, abscess and sputum (each < 5%).
2010	Texas, USA; February-June 2008/March-June2009[55]	Enterobacter cloacae	Children’s Hospital	Children ICU and Other Wards	3	Fecal colonization
2010	France; 2003-2004[56]	Klebsiella pneumoniae	care centre for abdominal surgery	ICU and Other Wards	8	Rectal swab, urine culture, blood culture, tracheal aspirates
2010	Athens, Greece; February - December 2009[57]	Klebsiella pneumoniae	University Hospital	ICU and Other Wards	42	Hospital-acquired infections
2010	Wuerzburg, Germany; November - December 2007[58]	Pseudomonas aeruginosa	retrograde urography associated infection	ICU and Other Wards	11	Strains from urine or urological infection
2010	Kobe, Japan; September 2007-July 2008[59]	Pseudomonas aeruginosa	Medical Center General Hospital	ICU patients	35	All patients from ICU
2011	Athens, Greece; March 2004 - November 2005[60]	Enterobacteriaceae	University Hospital	ICU patients	23	All patients from ICU
2011	Kasserine Tunisia; 2009 - June 2010[61]	Escherichia coli	University Hospital	ICU patients	2	Rectal swab
2011	Essen, Germany; July 2010 - January 2011[62]	Klebsiella pneumoniae	University Hospital	ICU and Other Wards	7	Perianal or rectal swabs
2011	Tunis, Tunisia; January - November 2008[63]	Pseudomonas aeruginosa	University Hospital	ICU and Other Wards	16	All patients of the kidney transplantation unit; 20 strains from urine, 3 from cutaneous pus, and 1 from blood
2011	Murcia, Spain; 11-25 May 2009[64]	Pseudomonas aeruginosa	Tertiary Care Hospital	ICU and Other Wards	6	4 ICU patients; strains from blood and sputum
2011	Central Japan; January 2006 - June 2009[65]	Pseudomonas aeruginosa	University Hospital	ICU and Other Wards	51	Mainly detected by urine culture in the first half, whereas isolation from respiratory tract samples became dominant in the latter half of the outbreak
2011	Rooterdam, Netherlands; January 2008 - November 2009[66]	Pseudomonas aeruginosa	University Hospital	ICU and Other Wards	35	161 carbapenemase-producing: 74 (70%) were isolated from respiratory tract specimens, 6 (6%) from urine, 5 (5%) from blood, 8 (8%) from soft tissue or bone, 7 (7%) from intra-abdominal specimens and 6 (6%) from various other specimens.
2012	Chosun, Korea; January 2004 - December 200[67]	Acinetobacter baumannii	University Hospital	ICU patients	77	All patients from ICU
2012	Madrid, Spain; January 2009 - December 2009[68]	Klebsiella pneumoniae	University Hospital	ICU patients	28	Fatality rate was 13/28 (46%)
2012	UK; 2005 – 2011[69]	Pseudomonas aeruginosa	Tertiary Care and University Hospitals	ICU and Other Wards	89	Fatality rate was 34/89 (38.2%)
2012	Cape Town, South Africa; January 2010 - April 2011[70]	Pseudomonas aeruginosa	Tertiary Care and University Hospitals	ICU patients	15	10 strains from blood, 2 from stool, 1 from bile, 1 from urine and 1 from a catheter tip
2013	Bologna, Italy; 1-15 June 2012[71]	Citrobacter freundii	University Hospital	ICU patients	8	Rectal swab
2013	Abidjan, Ivory Coast; February 2009 - November 2011[72]	Pseudomonas aeruginosa	University Hospital	ICU patients	12	All patients from ICU
2013	Thessalia, Larissa, Greece; 2010-2012[73]	Pseudomonas aeruginosa	University Hospital	ICU and Other Wards	49	All patients from ICU
2013	Taiwan; 2003-2007[74]	Pseudomonas aeruginosa	Regional Hospital	ICU and Other Wards	50	8 ICU patients
2013	Buenos Aires, Argentina; July–September 2011[19]	Serratia marcescens	Tertiary Care Neonatal University Hospital	Neonatal ward patients	3	Rectal swab; fatality rate was 1/2 (50%) and one lost at follow-up
2014	Split, Croatia; June - August 2012[75]	Enterobacter cloacae	University Hospital	ICU patients	6	Strains from lower respiratory tract, blood, abdominal cavity and rectum; fatality rate was 4/6 (66.6%)
2014	Greece; 2003-2007[76]	Klebsiella pneumoniae	Tertiary Care and University Hospitals	ICU patients	21	All patients from ICU
2014	Rome, Italy; 2011-2012[77]	Pseudomonas aeruginosa	Tertiary Care Paediatric Hospital	Children with onco-haematological diseases;	27	12 cases of bacteraemia, 6 other infections and 9 colonized; mortality rate was 67%
2014	Leiden, Netherlands; 2004- January 2012[78]	Pseudomonas aeruginosa	University Hospital	ICU patients	20	All patients from ICU
2014	China; December 2006 - July 2008[79]	Pseudomonas aeruginosa	Tertiary Care Hospitals	ICU patients	1	All patients from ICU
2015	Madrid, Spain - January 2009 - February 2014[80]	Klebsiella pneumoniae	University Hospital	ICU and Other Wards	37	OXA-48 ST11 clone
2015	Athens, Greece; September–November 2011[81]	Providencia stuartii	Tertiary Care Hospital	ICU patients	10/5	Strains from blood/urine; fatality rate was 7/15 (46.6%)
2015	Rotterdam, Netherlands; January - April 2012[82]	Pseudomonas aeruginosa	University Hospital	ICU and Other Wards	30	9 ICU patients; patients undergone ERCP using a specific duodenoscope (TJF-Q180V)
2015	UK, 2003 – 2012[83]	Pseudomonas aeruginosa	89 Tertiary Care Hospitals	ICU and Other Wards	267	Strains from urine (24%), respiratory (18%), wounds (17%) and blood (13%)
2016	Patras, Greece, January 2005 December 2014[84]	Klebsiella pneumoniae	University Hospital	ICU and Other Wards	45	1668 carbapenemase-producing isolates
2016	Athens, Greece; December 2012 - March 2013[85]	Providencia stuartii	Tertiary Care Hospital	ICU patients	6	Fatality rate was 3/6 (50%)
2016	China; August 2011-July 2012[86]	Pseudomonas aeruginosa	27 Tertiary Care Hospitals	ICU and Other Wards	49/44/42	Strains from pus/blood/urine
2017	Norway; 2007-2014[87]	Enterobacteriacee	University Hospital	ICU and Other Wards	14	Klebsiella pneumoniae (n = 10) and E. coli (n = 4)
2017	Jalisco, Mexico; September 2014 - July 2015[88]	Enterobacteriacee	Hospital Civil	ICU and Other Wards	3	Klebsiella pneumoniae (n=2), C. freundii (n = 1)
2017	Madrid, Spain - February 2014[89]	Klebsiella oxytoca	Children hospital	NICU	8	8 VIM-Kox/4 also had VIM-Serratia/3 patients VIM -Enterobacteriaceae. NICU, In neonates with any symptom of infection, urine, blood, broncho-alveolar lavages and other samples based on the most likely focus of infection
2017	UK; 2005-2011[90]	Pseudomonas aeruginosa	Two University Hospitals in London and South Coast	ICU and Other Wards	85	31 ICU patients; fatality rate was 34/85 (40%)
2018	Thessaloniki, Greece; January 2013- January 2015[91]	Klebsiella pneumoniae	University Hospital	ICU and Other Wards	25	Strain producing both KPC-2 and VIM-1 carbapenemases
2018	Cairo, Egypt, March 2015 August 2015[18]	Serratia marcescens	University Teaching Hospital	NICU	15	Isolates obtained from blood stream infections

ICU: Intensive care unit; NICU: Neonatal ICU. UK: United Kingdom; USA: United States of America.

Table 4 Previous hospital outbreaks of Serratia marcescens in Italy

Year	City	Setting	Number of cases(Infection and/or colonization)	Comments
1984	Naples[22]	NICU and Nursery	88	Outbreak linked to contaminated mucus aspiration apparatus and other contaminated instruments. Case fatality rate: 19%
1988	Genoa[23]	Adult ICU and surgical ward	11	Ventilators for assisted breathing became contaminated from index patient
1994	Varese[24]	Adult ICU	43	Strains from the ICU outbreak were multidrug resistance. 23 isolates from 18 other patients from other wards showed wide range of antibiotic susceptibility
2001	Naples[25]	NICU	14	56 cases of colonization by S marcescens over a 15-month period. Fourteen of the 56 colonized infants developed clinical infections, 50% of which were major (sepsis, meningitis, or pneumonia)
2003	Naples[26]	Adult ICU	13	Strain was multidrug resistant, inducible AmpC betalactamase producing. There were three cases of sepsis, nine pneumonia and one surgical wound infection. Mortality was 84.6%
2005	Modena[27]	NICU	15	Simultaneous outbreak of Serratia marcescens and Klebsiella pneumonia (11 cases). One preterm baby died in which both organisms were involved
2007	Pavia[9]	NICU	21	Occurred in two separate outbreaks in 10 mo interval
2009	Verona[28]	NICU	16	6 patients developed clinical diseases which included bacteremia, UTI, conjunctivitis and umbilical wound infection
2011	Pescara[7]	NICU	6	5 cases were linked toan index case hospitalised for S. marcescens sepsis. Mortality was 40%
2013	Modena[6]	NICU	127	Reported two long term outbreaks occurred over a period of 10 years. 43 developed infection and 3 died
2015	Floerence[5]	NICU	14	In the surveillance post outbreak, 18 out of 65 patients tested positive for S. marcescens

ICU: Intensive care unit; NICU: Neonatal ICU.