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©The Author(s) 2019.
World J Clin Cases. Oct 6, 2019; 7(19): 2930-2941
Published online Oct 6, 2019. doi: 10.12998/wjcc.v7.i19.2930
Published online Oct 6, 2019. doi: 10.12998/wjcc.v7.i19.2930
Table 1 Primer sequences of miR-489, miR-21, and internal reference U6
Group | Forward primer | Reverse primer |
miR-489 | 5'-ACACTCCAGCTGGGG TGACATCACATA-3' | 5'-TGGTGTCGTGGAGTCG-3' |
miR-21 | 5'-GCGGCGGTAGCTTATCAGACTG⁃3' | 5'-ATCCAGTGCAGGGTCCGAGG⁃3' |
U6 | 5'-CTCGCTTCGGCAGCACA-3' | 5'-AACGCTTCACGAATTTGCGT-3' |
Table 2 General clinical data of patients
Variable | Research group (n = 60) | Control group (n = 40) | t | P-value |
Age (yr) | 0.733 | 0.392 | ||
≤ 50 | 23 (38.33) | (30.00) | ||
> 50 | 37 (61.67) | 28 (70.00) | ||
Gender | 0.000 | 1.000 | ||
Male | 30 (50.00) | 20 (50.00) | ||
Female | 30 (50.00) | 20 (50.00) | ||
Tumor size (cm) | ||||
≤ 2 | 17 (28.33) | - | - | - |
> 2 | 43 (71.67) | - | - | - |
Lesion site | ||||
Non-acral | 44 (73.33) | - | - | - |
Acral | 16 (26.67) | - | - | - |
TNM stage | ||||
I | 7 (11.67) | - | - | - |
II | 8 (13.33) | - | - | - |
III | 25 (41.67) | - | - | - |
IV | 20 (33.33) | - | - | - |
Lymph node metastasis | ||||
Yes | 45 (75.00) | - | - | - |
No | 15 (25.00) | - | - | - |
Table 3 Relationship between expression of miR-489 and clinicopathological characteristics of melanoma
Variable | n | miR-489 | t/F | P-value |
Age (yr) | 1.341 | 0.185 | ||
≤ 50 | 23 | 0.43 ± 0.26 | ||
> 50 | 37 | 0.51 ± 0.20 | ||
Gender | 1.975 | 0.063 | ||
Male | 30 | 0.54 ± 0.18 | ||
Female | 30 | 0.42 ± 0.28 | ||
Tumor size (cm) | 0.439 | 0.779 | ||
≤ 2 | 17 | 0.45 ± 0.30 | ||
> 2 | 43 | 0.39 ± 0.16 | ||
Lesion site | 0.912 | 0.366 | ||
Non-acral | 44 | 0.50 ± 0.22 | ||
Acral | 16 | 0.44 ± 0.24 | ||
TNM stage | 4.269 | 0.009 | ||
I | 7 | 0.62 ± 0.29 | ||
II | 8 | 0.48 ± 0.24 | ||
III | 25 | 0.47 ± 0.22 | ||
IV | 20 | 0.31 ± 0.17 | ||
Metastasis | 5.622 | < 0.001 | ||
Yes | 45 | 0.30 ± 0.16 | ||
No | 15 | 0.64 ± 0.30 |
Table 4 Relationship between clinicopathological characteristics of melanoma and miR-21 expression
Variable | n | miR-21 | t/F | P-value |
Age (yr) | 1.984 | 0.052 | ||
≤ 50 | 23 | 4.72 ± 2.00 | ||
> 50 | 37 | 5.84 ± 2.20 | ||
Gender | 0.582 | 0.563 | ||
Male | 30 | 5.12 ± 1.75 | ||
Female | 30 | 5.44 ± 2.45 | ||
Tumor size (cm) | 0.386 | 0.873 | ||
≤ 2 | 17 | 4.99 ± 1.68 | ||
> 2 | 43 | 5.57 ± 2.52 | ||
Lesion site | 0.386 | 0.873 | ||
Non-acral | 44 | 5.16 ± 1.55 | ||
Acral | 16 | 5.40 ± 2.65 | ||
TNM stage | 5.040 | 0.004 | ||
I | 7 | 3.60 ± 1.79 | ||
II | 8 | 5.23 ± 2.13 | ||
III | 25 | 5.28 ± 2.01 | ||
IV | 20 | 7.01 ± 2.47 | ||
Metastasis | 3.295 | 0.002 | ||
Yes | 45 | 6.38 ± 2.35 | ||
No | 15 | 4.18 ± 1.85 |
Table 5 Diagnostic value of serum miR-489 and miR-21 in patients before treatment
Index | miR-489 | miR-21 |
AUC | 0.852 | 0.844 |
95%CI | 0.7678-0.9359 | 0.7633-0.9256 |
Std. Error | 0.0429 | 0.0414 |
Cut-off value | 0.451 | 4.841 |
Sensitivity (%) | 75.56 | 77.78 |
Specificity (%) | 80.00 | 82.22 |
Table 6 Assignment description of related factors of melanoma metastasis
Correlative factor | Assignment description |
Age (yr) | < 50 = 0; ≥ 50 = 1 |
Gender | Male = 0; female = 1 |
Tumor size (cm) | ≤ 2 = 0; > 2 = 1 |
Lesion site | Non-arcal = 0; arcal =1 |
TNM stage | I-II = 0; III-IV =1 |
miR-489 | < 0.451 = 0; > 0.451 = 1 |
miR-21 | < 4.841= 0; > 4.841 = 1 |
Table 7 Single-factor analysis of melanoma metastasis
Factor | Metastasis of melanoma (n = 45) | Melanoma is non-metastatic (n = 15) | t/X2 | P-value |
Age (yr) | 6.792 | 0.009 | ||
≤ 50 | 13 (28.89) | 10 (66.67) | ||
> 50 | 32 (71.11) | 5 (33.33) | ||
Gender | 2.222 | 0.136 | ||
Male | 25 (55.56) | 5 (33.33) | ||
Female | 20 (44.44) | 10 (66.67) | ||
Tumor size (cm) | 14.470 | < 0.001 | ||
≤ 2 | 7 (15.56) | 10 (66.67) | ||
> 2 | 38 (84.44) | 5 (33.33) | ||
Lesion site | 0.000 | 1.000 | ||
Non-acral | 33 (73.33) | 11 (73.33) | ||
Acral | 12 (26.67) | 4 (26.67) | ||
TNM stage | 35.920 | < 0.001 | ||
I | 0 (0.00) | 7 (46.67) | ||
II | 3 (6.67) | 5 (33.33) | ||
III | 22 (48.89) | 3 (20.00) | ||
IV | 20 (44.44) | 0 (0.00) | ||
miR-489 | 0.64 ± 0.30 | 0.30 ± 0.16 | 4.179 | < 0.001 |
miR-21 | 6.38 ± 2.35 | 4.18 ± 1.85 | 3.295 | 0.002 |
Table 8 Multivariate analysis of melanoma metastasis
Factor | β | SE | Wald | P | Exp (β) | 95%CI |
TNM stage | 1.393 | 0.752 | 4.172 | 0.041 | 4.890 | 1.038-19.304 |
miR-489 | 1.385 | 1.625 | 0.762 | 0.529 | 3.146 | 0.182-34.021 |
mir-21 | -2.037 | 1.232 | 6.514 | 0.038 | 0.391 | 0.059-1.449 |
- Citation: Mo H, Guan J, Yuan ZC, Lin X, Wu ZJ, Liu B, He JL. Expression and predictive value of miR-489 and miR-21 in melanoma metastasis. World J Clin Cases 2019; 7(19): 2930-2941
- URL: https://www.wjgnet.com/2307-8960/full/v7/i19/2930.htm
- DOI: https://dx.doi.org/10.12998/wjcc.v7.i19.2930