Observational Study
Copyright ©The Author(s) 2019.
World J Clin Cases. Sep 6, 2019; 7(17): 2463-2476
Published online Sep 6, 2019. doi: 10.12998/wjcc.v7.i17.2463
Table 1 Clinical characteristics of patients with ileal Crohn’s disease
PatientPt1Pt2Pt3Pt4Pt5Pt6Pt7Pt8Pt9Pt10Pt11Pt12Pt13Pt14
LocalizationL1L1L1L1L1L1L3L1L3L1L3-L4L1L3L1
of CDa
Age of CD5715535525313942162419184630
onset (yr)
Surgery / relapse1st1stRelapse1stRelapseRelapseRelapse1stRelapseRelapseRelapseRelapseRelapse1st
surgerysurgerysurgerysurgerysurgery
Disease behaviorbB2B2, B3B2B3B2, B4B2B3B2B2B2B2B2B3B2
TherapycC, F, I, BF, CCNoF, C, IC, F, II, BF, CC, I, F, BCF, C, BF, C, IC, F, B, IF, C, I
Smoking statusdNoCurNoNo10月10日NoNo20/3010月10日Cur10月20日NoNoNo
20/15.510
GenotypewtR702wthzG881RwtwtwtwthzG881RR702No seqNo seqwtwt
Table 2 Panel of the 24 genes investigated
SymbolComplete nameGroupAccession numbermimGene product function (s)Ref.
HPRT1Hypoxanthine phosphoribosyltransferase 1Low expression housekeeping geneM26434308000It plays a central role in the generation of purine nucleotides, chosen as a low expression housekeeping gene[39]
ACTBActin betaprovidedHigh expression housekeeping geneM28424102630Is involved in the cell motility, structure, and integrity[40]
SLC40A1Solute carrier family 40 (iron-regulated transporter), member11AF215636604653Exports iron from duodenal epithelial cells[41]
ABCB1ATP-binding cassette, sub-family B (MDR/TAP), member11M14758171050Transports various molecules across extra- and intra-cellular membranes. It belongs to a protein sub-family involved in multidrug resistance[42]
SLC22A5Solute carrier family 22 (organic cation/carnitine transporter), member 51AF057164603377Transports several small organic cations in the liver, kidney, intestine. It is involved in elimination of drugs and environmental toxins[43]
SLC22A4Solute carrier family 22 (organic cation/ergothioneine transporter), member 41AB007448604190Polyspecific transporter of organic cations in the liver, kidney, intestine, and involved in the elimination of these molecules.[44]
CCR6Chemokine (C-C motif) receptor62U68030601835Induces B-lineage maturation and antigen-driven B-cell differentiation[45]
IL17AInterleukin 17A2U32659603149Produced by Th17-type CD4+ cells. Regulates the activities of NF-kB and mitogen-activated protein kinases[26]
IL17FInterleukin 17F2AF384857606496Produced by Th17-type CD4+ cells. Stimulates the production of other cytokines, including IL6,IL8.It also inhibits angiogenesis by endothelial[46]
cells.
STAT3Signal transducer and activator of transcription 3 (acute-phase response factor)2-4BC014482102582Activates transcription of cell growth and apoptosis’ genes asresponses to inflammation[22,47]
MICAMHC class I polypeptide-related sequence A2L14848600169Acts as a stress-induced antigen broadly recognized by intestinal intra-epithelial gamma delta T cells.[48,49]
MYD88Myeloid differentiation primary response gene (88)2U84408602170Acts as an essential signal transducer in the interleukin-1 and Toll-like receptor signaling[50]
pathways
IL23RInterleukin 23 receptor2AF461422607562Expressed on Th17 cells. Involvedin the IL23A signaling pathways with the receptor molecule IL12RB1/IL12Rbeta1.[20]
JAK2Janus kinase 223717147796Is involved in cytokine receptor signaling pathways and is required for responses to gamma interferon[51]
IFNGInterferon, gamma23458147570It encodes a cytokine with antiviral,immunoregulatory and anti-tumor properties and activates macrophages[52]
CAMPCathelicidin antimicrobial peptide3BC055089600474It is an antimicrobial protein (defensin)[53]
CARD15Nucleotide-binding oligomerization domain containing 22AF178930605956Induces immune response to intracellular bacterial by recognizing the muramyl dipeptide (MDP)[54]
DEFB4Defensin, beta 4A3AJ314835602215Acts as an antibiotic peptide locally regulated by inflammation.[55,56]
HAMPHepcidin antimicrobialpeptide1/2/3AF309489606464It is involved in iron transport, antimicrobial, defence and inflammatory responses[57,58]
LRRK2Leucine-rich repeat kinase 23/4AK026776609007It is involved in autophagy and implicated in clearance of intracellular bacteria.[59,60]
TNFSF15Tumor necrosis facto (ligand) superfamily, member 154AF039390604052Induces apoptosis in endothelial cells[61]
CARD14Caspase recruitment domain family, member 144AF322642607211Regulates the molecular scaffolding process and activates NF-kappa B[62,63]
ATG16L1ATG16 autophagy related 16-like 14AK000897610767Induces autophagy processes involved in degradation of cell organelles[64]
ESR1Estrogen receptor 14X03635133430Involved in the metabolic pathway of the hormones and in several diseases including osteoporosis[65]
BMP2Bone morphogenetic protein 24650112261Induces bone and cartilage formation[66]
DLG5Discs, large homolog 54U61843604090It encodes for scaffolding molecules involved in cell-cell contact and in the maintenance of epithelial cell integrity. Its products are also involved in the transmission of extracellular signals[67]

  • Citation: Giudici F, Lombardelli L, Russo E, Cavalli T, Zambonin D, Logiodice F, Kullolli O, Giusti L, Bargellini T, Fazi M, Biancone L, Scaringi S, Clemente AM, Perissi E, Delfino G, Torcia MG, Ficari F, Tonelli F, Piccinni MP, Malentacchi C. Multiplex gene expression profile in inflamed mucosa of patients with Crohn’s disease ileal localization: A pilot study. World J Clin Cases 2019; 7(17): 2463-2476
  • URL: https://www.wjgnet.com/2307-8960/full/v7/i17/2463.htm
  • DOI: https://dx.doi.org/10.12998/wjcc.v7.i17.2463