Microvesicles with mitochondrial content are increased in patients with sepsis and associated with inflammatory responses

Table 1 Primers were used in this study for quantitative reverse transcription polymerase chain reaction

Gene	Forward primer	Reverse primer
IL-8	CTCTTGGCAGCCTTCCTGATT	TATGCACTGACATCTAAGTTCTTTAGCA
OAS2	ACGTGACATCCTCGATAAAACTG	GAACCCATCAAGGGACTTCTG
RSAD2	TGAGGTTCTGCAAAGTAGAGTT	GCGAGAATGTCCAAATACTCAC
CXCL10	GTGGCATTCAAGGAGTACCTC	TGATGGCCTTCGATTCTGGATT

Table 2 Patient Characteristics and plasma cytokine concentrations

	Control (n = 20)	Sepsis (n = 19)	Psepsis vs control
Characteristics
Agea	62.5 ± 9.8	58.13 ± 9.69	0.346
Male sexc	11	7	0.256
Hypertensionc	4	6	0.480
DMc	1	2	0.605
WBC, 109/Lb	6.18 ± 1.57	14.19 ± 8.02	< 0.001
Site of infection
Pneumonia	NA	6
Urinary	NA	6
Abdominal	NA	5
Bacteraemia	NA	2
Unknown	NA	3
Microbial data
Gram positive	NA	3
Gram negative	NA	13
Fungi	NA	1
Mixed	NA	2
Unknown	NA	4
Inflammation markers
TNF-α (pg/mL)b	6.90 (4.94-9.45)	26.10 (17.56-35.02)	< 0.001
IL-6 (pg/mL)b	7.33 (4.68-10.33)	61.25 (37.54-87.30)	< 0.001
IL-8 (pg/mL)b	7.30 (5.11–9.00)	19.59 (14.04-62.19)	< 0.001
CRP (ng/mL)b	158.62 (67.75-246.42)	445.07 (382.31-549.73)	< 0.001
sVCAM-1 (ng/mL)b	89.22 (72.09-99.67)	164.68 (134.15-198.55)	< 0.001

^aMean ± SD determined by t-test.

^bMedian (interquartile range) tested by the Kruskal-Wallis test.

^cThe rate or constituent ratio between the two groups was analyzed by the chi-square test.

IL-6: Interleukin-6; IL-8: Interleukin-8; TNF-α: Tumour necrosis factor-α; CRP: C-reactive protein; sVCAM-1: Soluble vascular cell adhesion molecule-1; NA: Not available.