In vitro maturation of human oocytes maintaining good development potential for rescue intracytoplasmic sperm injection with fresh sperm

Table 1 Comparison of the characteristics of the patients (female)

Variables	Old sperm group (n = 15)	Fresh sperm group (n = 13)	P value
Age (yr)	29.5 ± 2.1	30.6 ± 2.3	0.175
Infertility duration (yr)	3.4 ± 1.9	3.1 ± 2.0	0.546
Average Gn days	9.9 ± 3.2	11.1 ± 1.5	0.324
Average Gn consumption (IU)	2591.2 ± 1453.7	2076.6 ± 1267.3	0.532
BMI	21.2 ± 2.4	21.6 ± 2.7	0.301
Average oocyte number	12.1 ± 4.7	13.5 ± 4.9	0.326

Gn: Gonadotropin; BMI: Body mass index.

Table 2 Comparison of the characteristics of the patients (male)

Variables	Old sperm group(n = 15)	Fresh sperm group(n = 13)	P value
Age (yr)	32.5 ± 2.2	31.6 ± 2.3	0.324
Infertility duration (yr)	3.4 ± 1.9	3.1 ± 2.0	0.546
Motile sperm number (× 106)	3.2	2.9	0.245

Table 3 Comparison of the 24 h in vitro maturation rate of the two groups

Groups	No. of GV oocytes	No. of MII oocytes (24 h)	Maturation rate (%)
Old sperm group	102	52	51.0
Fresh sperm group	122	68	55.7a

^aP > 0.05 between the two groups.

GV: Germinal vesicle; MII: Metaphase-II.

Table 4 Comparison of in vitro maturation outcomes between the two groups

Variables	Old sperm Group	Fresh sperm Group	P value
2PN rate (%)	61.5 (32/52)	64.7 (44/68)	0.130
Cleavage rate (%)	93.8 (30/32)	93.2 (41/44)	1.00
day-3 top embryo rate (%)	16.6 (5/30)	63.4 (26/41)	0.001a
Useful blastocyst rate (%)	6.67 (2/30)	34.6 (9/26)	0.009a

^aP < 0.05 between the two groups.

2PN: Two pronuclear.