Development and validation of an epithelial–mesenchymal transition-related gene signature for predicting prognosis

Table 1 Gene sets enriched in lung adenocarcinoma

GS: follow link to MSigDB	Size	NES	Nom P value	FDR Q value
SARRIO_EPITHELIAL_MESENCHYMAL_TRANSITION_DN	144	-1.66	0.040	0.040
GOBP_CARDIAC_EPITHELIAL_TO_MESENCHYMAL_TRANSITION	30	-1.98	0.006	0.006
GOBP_EPITHELIAL_TO_MESENCHYMAL_TRANSITION	150	-1.71	0.045	0.045
GOBP_EPITHELIAL_TO_MESENCHYMAL_TRANSITION_INVOLVED_IN_ENDOCARDIAL_CUSHION_FORMATION	16	-2.05	< 0.001	< 0.001
GOBP_POSITIVE_REGULATION_OF_EPITHELIAL_TO_MESENCHYMAL_TRANSITION_INVOLVED_IN_ENDOCARDIAL_CUSHION_FORMATION	5	-1.65	0.006	0.006
GOBP_REGULATION_OF_EPITHELIAL_TO_MESENCHYMAL_TRANSITION_INVOLVED_IN_ENDOCARDIAL_CUSHION_FORMATION	6	-1.61	0.029	0.029
HOLLERN_EMT_BREAST_TUMOR_DN	121	1.90	0.020	0.020
JECHLINGER_EPITHELIAL_TO_MESENCHYMAL_TRANSITION_DN	62	-1.80	0.042	0.042
SARRIO_EPITHELIAL_MESENCHYMAL_TRANSITION_UP	166	2.03	0.008	0.008

GS: Gene set; MSigDB: Molecular Signatures Database; NES: Normalized enrichment score; NOM: Normalized; FDR: False discovery rate

Table 2 Clinical baseline data of lung adenocarcinoma patients

Variable	TCGA cohort	GEO cohort
Patients (n)	445	439
Sex
Female	245 (55.1%)	218 (49.7%)
Male	200 (44.9%)	221 (50.3%)
Age, yr
< 65	199 (44.7%)	213 (48.5%)
≥ 65	246 (55.3%)	226 (51.5%)
TNM stage
Ι	241 (54.2%)	274 (62.4%)
Ⅱ	107 (24.0%)	95 (21.6%)
Ⅲ	74 (16.4%)	67 (15.3%)
Ⅳ	24 (5.4%)	0
Unknown	0	3 (0.7%)
T stage
T1	154 (34.6%)	149 (33.9%)
T2	234 (52.6%)	248 (56.5%)
T3	37 (8.3%)	28 (6.4%)
T4	17 (3.8%)	11 (2.5%)
Tx	3 (0.7%)	3 (0.7%)
Survival status
OS time, median days	653	1418
Censored (%)	170 (38.2%)	233 (53.1%)

OS: Overall survival; TCGA: The Cancer Genome Atlas; GEO: Gene Expression Omnibus; TNM: Tumor, node and metastasis.

Table 3 Baseline data of the patients in the different risk groups

Variable	Derivation cohort			Validation cohort
	High risk	Low risk	P value	High risk	Low risk	P value
Sex			0.235			0.015
Female	116	129		96	122
Male	106	94		123	98
Age, yr			0. 742			0.416
< 65	101	98		102	111
≥ 65	121	125		117	109
TNM stage			0.001			0.017
Ι + Ⅱ	159	189		174	195
Ⅲ + Ⅳ	63	34		44	23
Unknown				1	2
T stage			0.002			< 0.001
T1	57	97		49	100
T2	132	102		142	106
T3	23	14		21	7
T4	9	8		6	5
Tx	1	2		1	2
Status			< 0.001			0.003
Surviving	116	159		87	119
Non-surviving	106	64		132	101

TNM: Tumor, node and metastasis.