Diffuse large B-cell lymphoma arising from follicular lymphoma with warthin’s tumor of the parotid gland - immunophenotypic and genetic features: A case report

Figure 1 Morphological characteristic of Warthin’s tumor and lymphoma. A: The bilayered oxyphilic, cuboidal or polygonal epithelium cells and lymphoid intraparernchymal components were observed; B, C: A lot of medium- to large- size lymphoid cells was observed diffusely in part of neoplasm (B) and a few secondary lymphoid follicles were seen at the centre or edge of neoplasm (C); D: At the border of epithelium, the lymphoepithelial lesions were identified. (hematoxylin-eosin staining, Magnification ×200)

Figure 2 Immunotype of lymphoma with Warthin’s tumor. A: The columnar oncocytic cells were positive for AE1/AE3 (Magnification ×100). B-E: The neoplastic lymphoid cells which were located incoarctate follicular were positive for CD20 (B), Pax-5 (C), bcl-2 (D), negative for CD10 (E); F, G: The adjacent diffusely medium- to large- size lymphoid cells was positive for CD20 (F), MUM-1(G); H: The Ki-67 proliferation index was estimated at approximately 80%. (Immunohistochemical stain, Magnification ×200).

Figure 3 Fluorescent in situ hybridization studies using dual-color dual fusion bcl-2 break apart rearrangement (18q21), bcl-6 break apart rearrangement (3q27), MYC (8q24), and MYC/IGH fusion translocation t (14;18) probes. A: bcl-2; B: bcl-6, abnormal cells with two break apart signals (red and green); C: MYC (8q24); D: MYC/IGH. (Magnification ×400).