Child with Wiskott–Aldrich syndrome underwent atypical immune reconstruction after umbilical cord blood transplantation: A case report

Figure 1 WBC changes after transplantation. A: Counts of WBCs, lymphocytes and neutrophils after UCB infusion. Counts of WBCs, lymphocytes and neutrophils changed and fluctuated near normal levels. WBC count reached 0.5 × 10⁹ on day 22, lymphocytes reached 0.5 × 10⁹ on day 23, and neutrophils reached 0.5 × 10⁹ on day 34; B: Percentage of neutrophils, lymphocytes and monocytes fluctuated near normal levels. The percentage of lymphocytes was high within 1 mo after transplantation; C: Changes in lymphocyte subsets after transplantation. The main subsets of lymphocytes in this patient were T cells; D: Changes in T cell subsets after transplantation. The main subgroup of T cells in this patient was CD4⁺ T cells; E: Percentage of lymphocytes from day −10 to day 50. After UCB infusion, percentage of lymphocytes changed and fluctuated near normal levels in different patients. UCB: Umbilical cord blood; WBC: White blood cell.

Figure 2 Reconstitution of DCs. A: Percentage of DCs and DC subsets day 27 to month 5. After UCB infusion, percentage of DCs, pDCs and CD1c⁺ DCs increased. DCs, lineage-HLA-DR⁺; plasmacytoid DCs, lineage-HLA-DR⁺CD123⁺; CD1c⁺ DCs, lineage-HLA-DR⁺CD1c⁺, detected by flow cytometry; B: DC changes after transplantation. Total DC reconstitution after transplantation; C: DC subset changes after transplantation. pDC and CD1c⁺ conventional DC reconstitution after transplantation; D: Delta-like protein 4 (DLL4⁺) DC changes after transplantation. After UCB infusion from day 27 to month 5, percentage of DLL4⁺ pDCs and DLL4⁺ CD1c⁺ DCs detected by flow cytometry after LPS (0.1 μg/mL) + R848 (0.1 μg/mL) stimulation for 24 h. DC: Dendritic cell; UCB: Umbilical cord blood.

Figure 3 Timeline of interventions and outcomes.