In vitro differentiation of human umbilical cord Wharton’s jelly mesenchymal stromal cells to insulin producing clusters

Figure 1 The Wharton’s Jelly mesenchymal stromal cells were grown from the edge of tissue explants (× 100).

Figure 2 Flow cytometry histogram of Wharton’s jelly derived mesenchymal stromal cells (CD90, CD105, CD44). WJ-MSCs are positive for these markers. Dark Blue lines indicate background fluorescence obtained with isotype control. WJ-MSCs: Wharton’s jelly derived mesenchymal stromal cells.

Figure 3 Fat droplets confirmed the adipogenic potential of Wharton’s

Figure 4 Calcium deposition confirmed the osteogenic potential of Wharton’s jelly derived mesenchymal stromal cells (Alizarin Red staining × 100).

Figure 5 Morphological differentiation of insulin producing clusters from Wharton’s jelly derived mesenchymal stromal cells in different stages (A-D × 100). A: WJ-MSCs were typically an adherent spindle shape; B and C: The cells gradually formed clusters in the medium 14 d after differentiation; D: At the end of final stage clusters were floated in the medium. WJ-MSCs: Wharton’s jelly derived mesenchymal stromal cells.

Figure 6 Dithizone staining. The differentiated cells stained as red color (× 100).

Figure 7 Expression of Ngn3, Insulin, PDX1, Oct4 and Nanog transcription factors were confirmed by electrophoresis.