Cutaneous leishmaniasis presenting with painless ulcer on the right forearm: A case report

Figure 1 Picture of the lesion. A: Overall view of the skin plaque with rupture on the right forearm; B: Local magnification of the right forearm plaque with ulcer. The black crust and translucent fluid were visible.

Figure 2 Pathological examination of the lesion. A: Hematoxylin and eosin section shows a large number of granular structures within the epithelial cells (40 ×); B: Giemsa staining revealed blue flagellar free bodies in tissue cells (40 ×); C: Giemsa staining revealed blue flagellar-free bodies in tissue cells of the secretion smear (100 ×).

Figure 3 Post-treatment follow-up. The plaques disappeared, the ulcer healed, and a scar remained.

Figure 4 Pathogen identification test. A: Polymerase chain reaction (PCR) amplification of ITS1. The PCR product can be seen as a single band with a size of 336 bp; B: Phylogenetic tree, in which ITS fetching sequences were the closest relatives to Leishmania major.