Integrative analysis of platelet-related genes for the prognosis of esophageal cancer

Figure 1 Identification of the candidate platelet-related genes in the esophageal cancer cohort. A: The heat map showed the expression of 369 platelet-related genes in healthy normal tissues and tumor tissues; B: Forest plots showed the results of the univariate Cox regression analysis between gene expression and overall survival; C: Venn diagram was used to identify differentially expressed genes between tumor and healthy normal tissue that were correlated with overall survival. DEGs: Differentially expressed genes.

Figure 2 Construction of a prognostic classifier based on platelet-associated genes. A and B: The results of the least absolute shrinkage and selection operator Cox regression suggest that four platelet-related genes were essential for the classifier; C: t-distributed stochastic neighbor embedding analysis; D: Principal component analysis plot. The expression levels of four biomarkers of the classifier in high- and low-risk group; E: Adenocarcinoma; F: Squamous cell carcinoma; G: All cases. t-SNE: t-distributed stochastic neighbor embedding.

Figure 3 Prognostic analysis of the 4-gene signature model in the esophagus cancer cohort. A: Distribution and median value of the risk scores; B: Distributions of overall survival status, overall survival, and risk score; C: Kaplan-Meier curves for the overall survival of patients in the high-risk group and low-risk group; D and E: Univariate and multivariate Cox regression analyses regarding overall survival; F: Area under the curve of time-dependent receiver operating characteristic curves.

Figure 4 Representative results of Gene Ontology circle plot and Kyoto Encyclopedia of Genes and Genomes analyses. A: The inner ring is a bar plot where the height of the bar indicates the significance of the term (log10 adjusted P value), and color corresponded to the Z-score. The outer ring displays scatterplots of the expression levels (log fold change) for the genes in each term. The table represents the distribution of platelet-related genes in significant gene ontology terms; B: Most significant or shared Kyoto Encyclopedia of Genes and Genomes pathways. FC: Fold change; GO: Gene ontology.

Figure 5 Construction of prognostic nomogram. A: Nomogram containing the risk score predicted the overall survival in patients with esophageal cancer; B: Receiver operating characteristic curves and area under the curve for 1-, 2-, and 3-year survival of the nomogram; C: Calibration curve of 1-, 2-, and 3-year survival in the nomogram and ideal model.