|
1
|
Barberoux V, Anzil A, Meinertzhagen L, Nguyen-Dinh T, Servais P, George IF. Spatio-temporal dynamics of bacterial community composition in a Western European watershed, the Meuse River watershed. FEMS Microbiol Ecol 2025; 101:fiaf022. [PMID: 40042978 PMCID: PMC11916896 DOI: 10.1093/femsec/fiaf022] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 10/31/2024] [Revised: 02/17/2025] [Accepted: 03/04/2025] [Indexed: 03/19/2025] Open
Abstract
This study aimed to identify factors influencing bacterial diversity in the Meuse River watershed by analyzing 42 locations sampled in spring and summer 2019, combined with biweekly sampling of one mid-stream location for a year. Bacterial community composition (BCC) was assessed in the small (SF; <5 µm) and large fractions (LF; ≥5 µm,), alongside physico-chemical parameters. LF consistently exhibited greater alpha diversity than SF. During the spatial campaigns, alpha diversity increased downstream in spring with high discharge, and BCC differed significantly between headwaters and the main river. Along this axis, several genera, Flavobacterium, Limnohabitans, and Aquirufa stood out as indicators of good water quality. Rhodoferax, another taxon indicative of good water quality, prevailed in the headwaters and during winter. In contrast, two cyanobacteria genera indicators of poor river quality, Microcystis PCC 7914 and Cyanobium PCC 6307, peaked in summer. BCC in spring and summer temporal samples aligned with spatial ones, while winter and autumn samples had distinct BCC. Finally, season, temperature, and distance from river mouth were the main driving parameters of beta diversity, outweighing the effect of fraction size on the BCC. These findings reinforce the notion that local conditions exert significant influence on bacterial communities in rivers.
Collapse
Affiliation(s)
- Valentin Barberoux
- Laboratory of Ecology of Aquatic Systems (ESA), Brussels Bioengineering School, Université Libre de Bruxelles, Brussels 1050, Belgium
- Laboratory of Marine Biology, Faculty of Sciences, Université Libre de Bruxelles, Brussels 1050, Belgium
| | - Adriana Anzil
- Laboratory of Ecology of Aquatic Systems (ESA), Brussels Bioengineering School, Université Libre de Bruxelles, Brussels 1050, Belgium
| | - Loïc Meinertzhagen
- Laboratory of Ecology of Aquatic Systems (ESA), Brussels Bioengineering School, Université Libre de Bruxelles, Brussels 1050, Belgium
| | - Thanh Nguyen-Dinh
- Greening Laboratory, Biomedicine Discovery Institute, Monash University, Victoria 3800, Australia
| | - Pierre Servais
- Laboratory of Ecology of Aquatic Systems (ESA), Brussels Bioengineering School, Université Libre de Bruxelles, Brussels 1050, Belgium
| | - Isabelle F George
- Laboratory of Ecology of Aquatic Systems (ESA), Brussels Bioengineering School, Université Libre de Bruxelles, Brussels 1050, Belgium
| |
Collapse
|
|
2
|
Shahid U, Hooi SL, Lim SY, Mohd Aris A, Khor BC, Ayub Q, Tan HS. Metagenomic surveillance of microbial community and antibiotic resistant genes associated with Malaysian wastewater during the COVID-19 pandemic. Curr Genet 2024; 71:1. [PMID: 39724227 DOI: 10.1007/s00294-024-01305-6] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 07/23/2024] [Revised: 12/13/2024] [Accepted: 12/16/2024] [Indexed: 12/28/2024]
Abstract
Wastewater is a reservoir of pathogens and hotspots for disseminating antibiotic resistance genes across species. The metagenomic surveillance of wastewater provides insight into the native microbial community, antibiotic-resistance genes (ARGs) and mobile genetic elements. t. The COVID-19 pandemic has caused wider dissemination of ARGs and resistant bacteria in wastewater. Although immensely significant, no research has been performed on the Malaysian wastewater microbial community and ARGs or their correlation with COVID-19 infections. This study utilised a 16S metagenomics approach to characterise the microbial community in Malaysian wastewater during high and low-case phases of the pandemic. Bacteria belonging to Bacteriodales, Bacillales, Actinomycetales and opportunistic pathogens-Arcobacters, Flavobacteria, and Campylobacterales, Neisseriales, were enriched during higher COVID-19 pandemic (active cases). Additionally, copy number profiling of ARGs in water samples showed the prevalence of elements conferring resistance to antibiotics like sulphonamides, cephalosporins, and colistin. The high prevalence of intI1 and other ion-based transporters in samples highlights an extensive risk of horizontal gene transfer to previously susceptible species. Our study emphasises the importance of wastewater surveillance in understanding microbial community dynamics and ARG dissemination, particularly during public health crises like the COVID-19 pandemic.
Collapse
Affiliation(s)
- Umama Shahid
- School of Science, Monash University Malaysia, Bandar Sunway, Selangor, 47500, Malaysia
| | - Suet Li Hooi
- School of Science, Monash University Malaysia, Bandar Sunway, Selangor, 47500, Malaysia
| | - Shu Yong Lim
- Monash University Malaysia Genomics Platform, Bandar Sunway, Selangor Darul Ehsan, 47500, Malaysia
| | - Alijah Mohd Aris
- Indah Water Research Centre, Indah Water Konsortium Sdn Bhd, No 1, Jalan Damansara, Kuala Lumpur, 60000, Malaysia
| | - Bee Chin Khor
- Indah Water Research Centre, Indah Water Konsortium Sdn Bhd, No 1, Jalan Damansara, Kuala Lumpur, 60000, Malaysia
| | - Qasim Ayub
- School of Science, Monash University Malaysia, Bandar Sunway, Selangor, 47500, Malaysia
- Monash University Malaysia Genomics Platform, Bandar Sunway, Selangor Darul Ehsan, 47500, Malaysia
| | - Hock Siew Tan
- School of Science, Monash University Malaysia, Bandar Sunway, Selangor, 47500, Malaysia.
| |
Collapse
|
|
3
|
Fu J, Wang J, Huang X, Guan B, Feng Q, Deng H. Composition and diversity of gut microbiota across developmental stages of Spodoptera frugiperda and its effect on the reproduction. Front Microbiol 2023; 14:1237684. [PMID: 37789854 PMCID: PMC10543693 DOI: 10.3389/fmicb.2023.1237684] [Citation(s) in RCA: 4] [Impact Index Per Article: 2.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 06/09/2023] [Accepted: 08/31/2023] [Indexed: 10/05/2023] Open
Abstract
Introduction Spodoptera frugiperda is a serious world-wide agricultural pest. Gut microorganisms play crucial roles in growth, development, immunity and behavior of host insects. Methods Here, we reported the composition of gut microbiota in a laboratory-reared strain of S. frugiperda using 16S rDNA sequencing and the effects of gut microbiota on the reproduction. Results Proteobacteria and Firmicutes were the predominant bacteria and the taxonomic composition varied during the life cycle. Alpha diversity indices indicated that the eggs had higher bacterial diversity than larvae, pupae and adults. Furthermore, eggs harbored a higher abundance of Ralstonia, Sediminibacterium and microbes of unclassified taxonomy. The dynamics changes in bacterial communities resulted in differences in the metabolic functions of the gut microbiota during development. Interestingly, the laid eggs in antibiotic treatment groups did not hatch much due to the gut dysbacteriosis, the results showed gut microbiota had a significant impact on the male reproduction. Discussion Our findings provide new perspectives to understand the intricate associations between microbiota and host, and have value for the development of S. frugiperda management strategies focusing on the pest gut microbiota.
Collapse
Affiliation(s)
- Junrui Fu
- Guangdong Key Laboratory of Insect Developmental Biology and Applied Technology, Guangzhou Key Laboratory of Insect Development Regulation and Application Research, Institute of Insect Science and Technology and School of Life Sciences, South China Normal University, Guangzhou, China
| | - Junhan Wang
- Guangdong Key Laboratory of Insect Developmental Biology and Applied Technology, Guangzhou Key Laboratory of Insect Development Regulation and Application Research, Institute of Insect Science and Technology and School of Life Sciences, South China Normal University, Guangzhou, China
| | - Ximei Huang
- Guangdong Key Laboratory of Insect Developmental Biology and Applied Technology, Guangzhou Key Laboratory of Insect Development Regulation and Application Research, Institute of Insect Science and Technology and School of Life Sciences, South China Normal University, Guangzhou, China
| | - Boyang Guan
- Guangdong Key Laboratory of Insect Developmental Biology and Applied Technology, Guangzhou Key Laboratory of Insect Development Regulation and Application Research, Institute of Insect Science and Technology and School of Life Sciences, South China Normal University, Guangzhou, China
| | - Qili Feng
- Guangdong Key Laboratory of Insect Developmental Biology and Applied Technology, Guangzhou Key Laboratory of Insect Development Regulation and Application Research, Institute of Insect Science and Technology and School of Life Sciences, South China Normal University, Guangzhou, China
| | - Huimin Deng
- Guangdong Key Laboratory of Insect Developmental Biology and Applied Technology, Guangzhou Key Laboratory of Insect Development Regulation and Application Research, Institute of Insect Science and Technology and School of Life Sciences, South China Normal University, Guangzhou, China
- Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, China
| |
Collapse
|
|
4
|
Sethuraman A, Stancheva R, Sanders C, Caceres L, Castro D, Hausknecht-Buss H, Henry S, Johansen H, Kasler A, Lastor S, Massaro I, Mekuria I, Moron-Solano A, Read N, Vengerova G, Zhang A, Zhang X, Read B. Genome of a novel Sediminibacterium discovered in association with two species of freshwater cyanobacteria from streams in Southern California. G3 GENES|GENOMES|GENETICS 2022; 12:6593491. [PMID: 35639673 PMCID: PMC9258536 DOI: 10.1093/g3journal/jkac123] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.3] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 11/20/2021] [Accepted: 04/28/2022] [Indexed: 11/30/2022]
Abstract
Here, we report the discovery of a novel Sediminibacterium sequenced from laboratory cultures of freshwater stream cyanobacteria from sites in Southern California, grown in BG11 medium. Our genome-wide analyses reveal a highly contiguous and complete genome (97% BUSCO) that is placed within sediminibacterial clades in phylogenomic analyses. Functional annotation indicates the presence of genes that could be involved in mutualistic/commensal relationship with associated cyanobacterial hosts.
Collapse
Affiliation(s)
- Arun Sethuraman
- Department of Biology, San Diego State University , San Diego, CA 92182, USA
- Department of Biological Sciences, California State University San Marcos , San Marcos, California USA 92096, USA
| | - Rosalina Stancheva
- Department of Biology, San Diego State University , San Diego, CA 92182, USA
| | - Ciara Sanders
- Department of Biology, San Diego State University , San Diego, CA 92182, USA
| | - Lakme Caceres
- Department of Biology, San Diego State University , San Diego, CA 92182, USA
- Palomar College , San Marcos, CA 92069, USA
- University of San Diego , San Diego, CA 92110, USA
| | - David Castro
- Department of Biology, San Diego State University , San Diego, CA 92182, USA
| | - Hannah Hausknecht-Buss
- Department of Biology, San Diego State University , San Diego, CA 92182, USA
- Scripps College , Claremont, CA 91711, USA
| | - Simone Henry
- Department of Biology, San Diego State University , San Diego, CA 92182, USA
- Scripps College , Claremont, CA 91711, USA
| | - Haven Johansen
- Department of Biology, San Diego State University , San Diego, CA 92182, USA
- Western Washington University , Bellingham, WA 98225, USA
| | - Antolette Kasler
- Department of Biology, San Diego State University , San Diego, CA 92182, USA
| | - Sandy Lastor
- Department of Biology, San Diego State University , San Diego, CA 92182, USA
- Bay Path University , Long Meadow, MA 01106, USA
| | - Isabelle Massaro
- Department of Biology, San Diego State University , San Diego, CA 92182, USA
| | - Immanuel Mekuria
- Department of Biology, San Diego State University , San Diego, CA 92182, USA
| | - Andrea Moron-Solano
- Department of Biology, San Diego State University , San Diego, CA 92182, USA
- University of California , Davis, Davis, CA 95616, USA
| | - Niki Read
- Department of Biology, San Diego State University , San Diego, CA 92182, USA
| | - Gretchen Vengerova
- Department of Biology, San Diego State University , San Diego, CA 92182, USA
- City College of San Francisco , San Francisco, CA 94112, USA
| | - Andrew Zhang
- Department of Biology, San Diego State University , San Diego, CA 92182, USA
- Indiana University Bloomington , Bloomington, IN 47405, USA
| | - Xiaoyu Zhang
- Department of Biology, San Diego State University , San Diego, CA 92182, USA
| | - Betsy Read
- Department of Biology, San Diego State University , San Diego, CA 92182, USA
| |
Collapse
|
|
5
|
Tan XL, Chen X, Fu YJ, Ye L, Zhang L, Huang DM. Diverse microbiota in palatal radicular groove analyzed by Illumina sequencing: Four case reports. World J Clin Cases 2021; 9:6846-6857. [PMID: 34447834 PMCID: PMC8362521 DOI: 10.12998/wjcc.v9.i23.6846] [Citation(s) in RCA: 2] [Impact Index Per Article: 0.5] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 04/14/2021] [Revised: 05/24/2021] [Accepted: 06/04/2021] [Indexed: 02/06/2023] Open
Abstract
BACKGROUND A palatal radicular groove is an unusual developmental deformity of the tooth, which may serve as a channel linking the periodontal and periapical inflammation, and yet no literature could be obtained analyzing microbiota within the palatal radicular grooves.
CASE SUMMARY Four patients diagnosed with palatal radicular groove and concomitant periodontal-endodontic deformity in permanent maxillary lateral incisors were enrolled in this work. Twelve bacterial samples from 4 patients were collected from different parts of the palatal radicular groove during intentional replantation surgery. Illumina sequencing was performed to analyze the taxonomical composition and microbiome structure inside the palatal grooves, and 1162 operational taxonomic units were obtained. The phyla of Firmicutes and Proteobacteria predominated in most of the samples. An unknown genus from the Bacillaceae family, Lactococcus, and Porphyromonas were the most abundant genera identified. There was no difference in the microbiota richness and diversity in three sections of the groove.
CONCLUSION The unique ecological niches inside the palatal grooves harbored bacterial communities that shared some component features of both the endodontic and periodontal infections. The existence of palatal groove may play an interaction bridge between the root apex and tooth cervix and thus impair the outcome of traditional therapeutic methods such as root canal treatment and periodontal management.
Collapse
Affiliation(s)
- Xue-Lian Tan
- State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & Department of Operative Dentistry and Endodontics West China Hospital of Stomatology, Sichuan University, Chengdu 610041, Sichuan Province, China
| | - Xuan Chen
- State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & Department of Operative Dentistry and Endodontics West China Hospital of Stomatology, Sichuan University, Chengdu 610041, Sichuan Province, China
| | - Yu-Jie Fu
- State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & Department of Operative Dentistry and Endodontics West China Hospital of Stomatology, Sichuan University, Chengdu 610041, Sichuan Province, China
| | - Ling Ye
- State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & Department of Operative Dentistry and Endodontics West China Hospital of Stomatology, Sichuan University, Chengdu 610041, Sichuan Province, China
| | - Lan Zhang
- State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & Department of Operative Dentistry and Endodontics West China Hospital of Stomatology, Sichuan University, Chengdu 610041, Sichuan Province, China
| | - Ding-Ming Huang
- State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & Department of Operative Dentistry and Endodontics West China Hospital of Stomatology, Sichuan University, Chengdu 610041, Sichuan Province, China
| |
Collapse
|
|
6
|
Seasonal Distribution of Cyanobacteria in Three Urban Eutrophic Lakes Results from an Epidemic-like Response to Environmental Conditions. Curr Microbiol 2021; 78:2298-2316. [PMID: 33904973 DOI: 10.1007/s00284-021-02498-6] [Citation(s) in RCA: 2] [Impact Index Per Article: 0.5] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 01/13/2021] [Accepted: 04/16/2021] [Indexed: 10/21/2022]
Abstract
Cyanobacterial communities of three co-located eutrophic sandpit lakes were surveyed during 2016 and 2017 over season and depth using high-throughput DNA sequencing of the 16S rRNA gene. All three lakes were stratified except during April 2017 when the lakes were recovering from a strong mixing event. 16S rRNA gene V4 sequences were parsed into operational taxonomic units (OTUs) at 99% sequence identity. After rarefaction of 139 samples to 25,000 sequences per sample, a combined total of 921,529 partial 16S rRNA gene sequences were identified as cyanobacteria. They were binned into 19,588 unique cyanobacterial OTUs. Of these OTUs, 11,303 were Cyanobium. Filamentous Planktothrix contributed 1537 and colonial Microcystis contributed 265. The remaining 6482 OTUs were considered unclassified. For Planktothrix and Microcystis one OTU accounted for greater than 95% of the total sequences for each genus. However, in both cases the non-dominant OTUs clustered with the dominant OTUs by date, lake, and depth. All Planktothrix OTUs and a single Cyanobium OTU were detected below the oxycline. All other Cyanobium and Microcystis OTUs were detected above the oxycline. The distribution of Cyanobium OTUs between lakes and seasons can be explained by an epidemic-like response where individual OTUs clonally rise from a diverse hypolimnion population when conditions are appropriate. The importance of using 99% identity over the more commonly used 97% is discussed with respect to cyanobacterial community structure. The approach described here can provide another valuable tool for assessing cyanobacterial populations and provide greater insight into the controls of cyanobacterial blooms.
Collapse
|
|
7
|
Wu S, Zhong L, Liao S, Li T, Zhou Z, Wang G. Sediminibacterium soli sp. nov., isolated from soil. Arch Microbiol 2020; 203:967-973. [PMID: 33104819 DOI: 10.1007/s00203-020-02089-2] [Citation(s) in RCA: 7] [Impact Index Per Article: 1.4] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 07/14/2020] [Revised: 10/06/2020] [Accepted: 10/08/2020] [Indexed: 11/25/2022]
Abstract
A Gram-stain-negative, facultative anaerobic strain, designated WSJ-3T, was isolated from soil. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain WSJ-3T belongs to genus Sediminibacterium and exhibits the highest sequence similarities to Sediminibacterium roseum SYL130T (97.0%), Sediminibacterium goheungense DSM 28323T (96.9%), Sediminibacterium aquarii AA5T (96.7%), and Sediminibacterium salmoneum NBRC 103935T (95.2%). The average nucleotide identity values of strain WSJ-3T/S. roseum SYL130T and strain WSJ-3T/S. goheungense DSM 28323T are 72.2% and 70.4%, respectively, and digital DNA-DNA hybridization values for these are 19.2% and 19.1%, respectively. Strain WSJ-3T has a genome size of 3.88 Mb, with a DNA G + C content of 50.1 mol% and comprises of 3263 predicted genes. A phylogenetic tree constructed using the genomic core protein coding sequences revealed that strain WSJ-3T clusters with S. roseum SYL130T. Strain WSJ-3T has menaquinone-7 as the only respiratory quinone and phosphatidylethanolamine, three unidentified phospholipids, four unidentified aminophospholipids, two unidentified aminolipids, and three unidentified lipids as the polar lipids. The major fatty acids of strain WSJ-3T are iso-C15:0, iso-C17:0 3-OH, and iso-C15:1 G. On the basis of the polyphasic results, the isolate represents a novel species of the genus Sediminibacterium, for which the name Sediminibacterium soli sp. nov. is proposed. The type strain is WSJ-3T (= KCTC 72839T = CCTCC AB 2019408T).
Collapse
Affiliation(s)
- Shijuan Wu
- State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070, Hubei, People's Republic of China
| | - Limin Zhong
- State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070, Hubei, People's Republic of China
| | - Shuijiao Liao
- State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070, Hubei, People's Republic of China
| | - Tengfei Li
- State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070, Hubei, People's Republic of China
| | - Zijie Zhou
- State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070, Hubei, People's Republic of China
| | - Gejiao Wang
- State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070, Hubei, People's Republic of China.
| |
Collapse
|
|
8
|
Zhang L, Long B, Wu J, Cheng Y, Zhang B, Zeng Y, Huang S, Zeng M. Evolution of microbial community during dry storage and recovery of aerobic granular sludge. Heliyon 2019; 5:e03023. [PMID: 31890963 PMCID: PMC6926229 DOI: 10.1016/j.heliyon.2019.e03023] [Citation(s) in RCA: 14] [Impact Index Per Article: 2.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/12/2019] [Revised: 09/18/2019] [Accepted: 12/06/2019] [Indexed: 12/15/2022] Open
Abstract
Aerobic granular sludge (AGS) was imbedded in agar and stored at 4 °C for 30 days, and then the stored granules were recovered in a sequencing batch reactor fed real wastewater within 11 days. Variations in microbial community compositions were investigated during dry storage and recovery of AGS, aiming to elucidate the mechanism of granular stability loss and recovery. The storage and recovery of AGS involved microbial community evolution. The dominant bacterial genera of the mature AGS were Zoogloea (relative abundance of 22.39%), Thauera (16.03%) and Clostridium_sensu_stricto (11.17%), and those of the stored granules were Acidovorax (26.79%), Macellibacteroides (12.83%) and Pseudoxanthomonas (5.69%), respectively. However, the dominant genera were Streptococcus (43.64%), Clostridium_sensu_stricto (12.3.6%) and Lactococcus (11.47%) in the recovered AGS. Methanogens were always the dominant archaeal species in mature AGS (93.01%), stored granules (99.99%) and the recovered AGS (94.84%). Facultative anaerobes and anaerobes proliferated and dominated in the stored granules, and their metabolic activities gradually led to granular structure destruction and property deterioration. However, the stored granules served as carriers for the microbes originated from the real septic tank wastewater during recovery. They proliferated rapidly and secreted a large number of extracellular polymeric substances which helped to recover the granular structure in 11 days.
Collapse
Affiliation(s)
- Linan Zhang
- School of Architectural and Surveying & Mapping Engineering, Jiangxi University of Science and Technology, Hongqi Ave. 86, Ganzhou, 341000, Jiangxi, China
| | - Bei Long
- School of Architectural and Surveying & Mapping Engineering, Jiangxi University of Science and Technology, Hongqi Ave. 86, Ganzhou, 341000, Jiangxi, China
| | - Junfeng Wu
- Henan Province Key Laboratory of Water Pollution Control and Rehabilitation Technology, Mingyue Road, Pingdingshan, 467036, Henan, China
| | - Yuanyuan Cheng
- School of Architectural and Surveying & Mapping Engineering, Jiangxi University of Science and Technology, Hongqi Ave. 86, Ganzhou, 341000, Jiangxi, China
| | - Binchao Zhang
- School of Architectural and Surveying & Mapping Engineering, Jiangxi University of Science and Technology, Hongqi Ave. 86, Ganzhou, 341000, Jiangxi, China
| | - Yu Zeng
- School of Architectural and Surveying & Mapping Engineering, Jiangxi University of Science and Technology, Hongqi Ave. 86, Ganzhou, 341000, Jiangxi, China
| | - Sinong Huang
- School of Architectural and Surveying & Mapping Engineering, Jiangxi University of Science and Technology, Hongqi Ave. 86, Ganzhou, 341000, Jiangxi, China
| | - Mingjing Zeng
- School of Architectural and Surveying & Mapping Engineering, Jiangxi University of Science and Technology, Hongqi Ave. 86, Ganzhou, 341000, Jiangxi, China
| |
Collapse
|
|
9
|
Song Z, Song Y, Yu Y, Choi L, Wang G, Li M. Dyadobacter luticola sp. nov., isolated from a sewage sediment sample. Int J Syst Evol Microbiol 2018; 69:465-469. [PMID: 30566070 DOI: 10.1099/ijsem.0.003178] [Citation(s) in RCA: 9] [Impact Index Per Article: 1.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/18/2022] Open
Abstract
A Gram-stain-negative, non-motile, aerobic bacterial strain, designated T17T was isolated from a sample of sewage sediment from a Busan park (Republic of Korea). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain T17T had the highest 16S rRNA gene sequence similarity to Dyadobacter soli KCTC 22481T (97.3 %), D.yadobacter fermentans DSM 18053T (97.1 %) and D.yadobacter sediminis CGMCC 1.12895T (97.1 %). The isolate exhibited relatively low levels of DNA-DNA relatedness with respect to D. soli KCTC 22481T (28.2±3.6 %). The DNA G+C content was 49.1 mol%. The unique respiratory quinone was MK-7 and the major polar lipids were phosphatidylethanolamine, five unidentified lipids, four aminolipids, two unidentified phospholipids and one glycophospholipid. The predominant cellular fatty acids (>5 % of total) were summed feature 3 (C16 : 1ω7c and/or iso-C15 : 0 2-OH; 44.3 %), iso-C15 : 0 (15.7 %), C16 : 1ω5c (9.6 %), iso-C17 : 0 3-OH (9.3 %) and C16 : 0 (5.6 %). Moreover, physiological and biochemical characteristics distinguished strain T17T from its related species, including temperature and pH ranges for growth, being positive for acetate hydrolysis, and being negative for acid produced from melibiose and rhamnose. The genotypic, chemotaxonomic and phenotypic data revealed that strain T17T represents a novel species of the genus Dyadobacter, for which the name Dyadobacter luticola sp. nov. is proposed. The type strain is T17T (=KCTC 52981T=CCTCC AB 2017091T).
Collapse
Affiliation(s)
- Zhirui Song
- State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, PR China
| | - Yali Song
- State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, PR China
| | - Ying Yu
- State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, PR China
| | - Lina Choi
- State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, PR China
| | - Gejiao Wang
- State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, PR China
| | - Mingshun Li
- State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, PR China
| |
Collapse
|