Basic Study
Copyright ©The Author(s) 2016. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Methodol. Sep 26, 2016; 6(3): 190-199
Published online Sep 26, 2016. doi: 10.5662/wjm.v6.i3.190
Adjuvant role of Pseudomonas flagellin for Acinetobacter baumannii biofilm associated protein
Mozhgan Derakhshan Sefidi, Iraj Rasooli, Parviz Owlia, Daryush Talei, Shakiba Darvish Alipour Astaneh, Shahram Nazarian
Mozhgan Derakhshan Sefidi, Iraj Rasooli, Department of Biology, Shahed University, Tehran 3319118651, Iran
Iraj Rasooli, Parviz Owlia, Molecular Microbiology Research Center, Shahed University, Tehran 3319118651, Iran
Daryush Talei, Shahed University, Tehran 3319118651, Iran
Shakiba Darvish Alipour Astaneh, Department of Biotechnology, Semnan University, Semnan 3513119111, Iran
Shahram Nazarian, Department of Biology, Faculty of Science, Imam Hussein University, Tehran 3319118651, Iran
Author contributions: Rasooli I contributed to the conception and design of the study, or acquisition of data, or analysis and interpretation of data; Sefidi MD carried out the experimental part of the project; Owlia P revised it critically for important intellectual content; Talei D carried out the statistical analyses; Astaneh SDA supervised the experiments; Nazarian S drafted the article; and final approval of the version to be submitted was made by Rasooli I.
Institutional review board statement: This work was carried out as a part of MSc thesis of Ms. Mozhgan Derakhshan Sefidi at Shahed University. The thesis proposal was approved by Post Graduate Committee in Biology Department of the Aforementioned University.
Institutional animal care and use committee statement: The animal care protocol was approved by Shahed University. 4-6 wk old (16-22 g) BALB/c mice procured from the Razi Institute, Tehran, Iran were housed in clean standard animal care facility of Shahed University. The research was carried out in compliance with the Animal Welfare Act and regulations related to experiments involving animals. The animal protocol was designed to minimize pain or discomfort to the animals. The animals were acclimatized to laboratory conditions (23 °C, 12 h/12 h light/dark, 50% humidity, ad libitum access to food and water) for two weeks prior to experimentation. All animals were euthanized by barbiturate overdose (intravenous injection, 150 mg/kg pentobarbital sodium) for tissue collection.
Conflict-of-interest statement: The authors declare no conflict of interests.
Data sharing statement: Technical details and dataset available from the corresponding author at rasooli@shahed.ac.ir. No additional data are available.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Correspondence to: Iraj Rasooli, Professor, Department of Biology, Shahed University, Tehran-Qom Express way, Tehran 3319118651, Iran. rasooli@shahed.ac.ir
Telephone: +98-21-51212200 Fax: +98-21-51212201
Received: April 12, 2016
Peer-review started: April 15, 2016
First decision: May 19, 2016
Revised: June 19, 2016
Accepted: July 14, 2016
Article in press: July 16, 2016
Published online: September 26, 2016
Processing time: 160 Days and 1.3 Hours
Core Tip

Core tip: The increasing frequency of Acinetobacter baumannii (A. baumannii) infections and its drug resistance challenge health authorities. Flagellin is an effective immune activator stimulating various biologic functions identified by Toll like receptor 5. Conserved regions of biofilm associated protein (Bap) have already been identified and their immunoprotectivity against A. baumannii have been established. In order to enhance their immunogenic activities, we designed a study on adjuvant role of flagellin from Pseudomonas aeruginosa for Bap.