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Lorubbio M, Diamanti D, Pieroni C, Gialli E, Pettinari M, Bassi S, Gorini G, Carniani S, Saracini A, Meloni P, Chiodi M, Gervino S, Pantone P, Ognibene A. Erythrocyte Sedimentation Rate Reference Intervals Determined via VES-MATIC 5 and CUBE 30 Touch with Respect to the Westergren Method. Diagnostics (Basel) 2025; 15:1101. [PMID: 40361919 PMCID: PMC12071782 DOI: 10.3390/diagnostics15091101] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/30/2025] [Revised: 04/21/2025] [Accepted: 04/23/2025] [Indexed: 05/15/2025] Open
Abstract
Objectives: The erythrocyte sedimentation rate (ESR) is a diagnostic test that is employed worldwide to assess a patient's inflammatory status. Like all laboratory tests, it requires reference intervals (RIs) to support clinical decision making and facilitate accurate diagnosis. In this study, we aimed to generate RIs for the automatic analyzers VES-MATIC 5 (VM5) and CUBE 30 touch (C30T) compared to the gold standard method. Methods: A total of 989 (presumably healthy) participants from Arezzo Hospital in Italy were enrolled. The ESR RIs were established according to CLSI for all three methods. Results: The analysis pointed out significant differences between women and men and age-related increases in ESRs obtained via all three analytical methods. The average and median values resulting from VM5 and C30T were, respectively, 1 mm/h smaller and higher than the gold standard. The RIs were calculated based on three clusters: the first pertained to patients aged ≥ 18 but ≤ 49 years; the second pertained to patients aged ≥ 50 but ≤ 69 years; the last comprised patients aged ≥ 70 years. Due to the clear overlap between these ranges and the statistical analysis, we identified only one range for females ≥ 18 years (Westergren: 1-22 mm/h; VM5: 1-22 mm/h; C30T: 1-25 mm/h). For the male participants, two separate RIs were proposed: one for those aged ≥ 18 but < 69 years (Westergren: 1-14 mm/h; VM5: 1-14 mm/h; C30T: 1-18 mm/h) and one for those aged 70 years or above (Westergren: 1-22 mm/h; VM5: 1-23 mm/h; C30T: 1-29 mm/h). Conclusions: The proposed RI for automated analyzers C30T and VM5 agreed with the reference method and can be adopted to measure ESRs within EDTA blood samples.
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Affiliation(s)
- Maria Lorubbio
- UOC Laboratory Medicine, Department of Laboratory Medicine and Transfusion, San Donato Hospital, 52100 Arezzo, Italy; (E.G.); (M.P.); (S.B.); (G.G.); (S.C.); (A.S.); (P.M.); (M.C.); (S.G.); (P.P.); (A.O.)
| | - Daniela Diamanti
- Diesse-Diagnostica Senese S.p.A., 53035 Monteriggioni, Italy; (D.D.); (C.P.)
| | - Carolina Pieroni
- Diesse-Diagnostica Senese S.p.A., 53035 Monteriggioni, Italy; (D.D.); (C.P.)
| | - Elena Gialli
- UOC Laboratory Medicine, Department of Laboratory Medicine and Transfusion, San Donato Hospital, 52100 Arezzo, Italy; (E.G.); (M.P.); (S.B.); (G.G.); (S.C.); (A.S.); (P.M.); (M.C.); (S.G.); (P.P.); (A.O.)
| | - Massimiliano Pettinari
- UOC Laboratory Medicine, Department of Laboratory Medicine and Transfusion, San Donato Hospital, 52100 Arezzo, Italy; (E.G.); (M.P.); (S.B.); (G.G.); (S.C.); (A.S.); (P.M.); (M.C.); (S.G.); (P.P.); (A.O.)
| | - Stefania Bassi
- UOC Laboratory Medicine, Department of Laboratory Medicine and Transfusion, San Donato Hospital, 52100 Arezzo, Italy; (E.G.); (M.P.); (S.B.); (G.G.); (S.C.); (A.S.); (P.M.); (M.C.); (S.G.); (P.P.); (A.O.)
| | - Gabriele Gorini
- UOC Laboratory Medicine, Department of Laboratory Medicine and Transfusion, San Donato Hospital, 52100 Arezzo, Italy; (E.G.); (M.P.); (S.B.); (G.G.); (S.C.); (A.S.); (P.M.); (M.C.); (S.G.); (P.P.); (A.O.)
| | - Stefania Carniani
- UOC Laboratory Medicine, Department of Laboratory Medicine and Transfusion, San Donato Hospital, 52100 Arezzo, Italy; (E.G.); (M.P.); (S.B.); (G.G.); (S.C.); (A.S.); (P.M.); (M.C.); (S.G.); (P.P.); (A.O.)
| | - Alessandro Saracini
- UOC Laboratory Medicine, Department of Laboratory Medicine and Transfusion, San Donato Hospital, 52100 Arezzo, Italy; (E.G.); (M.P.); (S.B.); (G.G.); (S.C.); (A.S.); (P.M.); (M.C.); (S.G.); (P.P.); (A.O.)
| | - Paola Meloni
- UOC Laboratory Medicine, Department of Laboratory Medicine and Transfusion, San Donato Hospital, 52100 Arezzo, Italy; (E.G.); (M.P.); (S.B.); (G.G.); (S.C.); (A.S.); (P.M.); (M.C.); (S.G.); (P.P.); (A.O.)
| | - Michela Chiodi
- UOC Laboratory Medicine, Department of Laboratory Medicine and Transfusion, San Donato Hospital, 52100 Arezzo, Italy; (E.G.); (M.P.); (S.B.); (G.G.); (S.C.); (A.S.); (P.M.); (M.C.); (S.G.); (P.P.); (A.O.)
| | - Silvana Gervino
- UOC Laboratory Medicine, Department of Laboratory Medicine and Transfusion, San Donato Hospital, 52100 Arezzo, Italy; (E.G.); (M.P.); (S.B.); (G.G.); (S.C.); (A.S.); (P.M.); (M.C.); (S.G.); (P.P.); (A.O.)
| | - Pietro Pantone
- UOC Laboratory Medicine, Department of Laboratory Medicine and Transfusion, San Donato Hospital, 52100 Arezzo, Italy; (E.G.); (M.P.); (S.B.); (G.G.); (S.C.); (A.S.); (P.M.); (M.C.); (S.G.); (P.P.); (A.O.)
| | - Agostino Ognibene
- UOC Laboratory Medicine, Department of Laboratory Medicine and Transfusion, San Donato Hospital, 52100 Arezzo, Italy; (E.G.); (M.P.); (S.B.); (G.G.); (S.C.); (A.S.); (P.M.); (M.C.); (S.G.); (P.P.); (A.O.)
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Issa IA, Issa T. Assessing endoscopic remission in small bowel Crohn's disease: Are markers enough? World J Gastrointest Endosc 2025; 17:106083. [PMID: 40291128 PMCID: PMC12019123 DOI: 10.4253/wjge.v17.i4.106083] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 02/17/2025] [Revised: 03/23/2025] [Accepted: 04/03/2025] [Indexed: 04/14/2025] Open
Abstract
Mucosal healing in Crohn's disease (CD) has been established as a crucial target of treatment, leading to long term remission and decrease in complication rates. Endoscopy still serves as the gold standard for assessment, particularly in the small bowel where balloon or capsule enteroscopy is frequently needed. However, these modalities are often unavailable, expensive, and invasive, posing risks to patients. Consequently, the identification of accessible and reliable biomarkers, especially in small intestinal CD, remains a challenge. The study by Ohno et al, published in this issue, further illuminates this field. It confirms the potential role of fecal biomarker leucine-rich α2 glycoprotein (LRG) and validates findings from previous smaller trials. Comparing to other markers LRG showed a much higher predictive value for mucosal healing of the small bowel, making it a useful option for small intestinal CD follow up. In this editorial, we explore the optimal marker of inflammation or mucosal healing in CD, particularly in the small bowel. We provide an overview of available conventional biomarkers and introduce several novel biomarkers, including an update on emerging technologies and innovations.
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Affiliation(s)
- Iyad A Issa
- Department of Gastroenterology and Hepatology, Harley Street Medical Center, Abu Dhabi 41475, United Arab Emirates
| | - Taly Issa
- Medical School, University of Nicosia, Nicosia 24005, Lefkosía, Cyprus
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Kasiak P, Kowalski T, Faiss R, Malczewska-Lenczowska J. Hemoglobin mass is accurately predicted in endurance athletes. J Sports Sci 2025; 43:289-298. [PMID: 39819560 DOI: 10.1080/02640414.2025.2453347] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 01/19/2025]
Abstract
Hemoglobin mass (Hbmass) prediction enhance the accessibility and practicality of athletes' hemoglobin status monitoring, facilitating better performance. Therefore, we aimed to create prediction equations for Hbmass in well-trained endurance athletes (EA), based on easily obtained measures. The population of 220 well-trained EA (40% females, maximal oxygen uptake = 63.4 ± 8.00 mL·kg·min-1) was randomly split for the models' derivation and validation in 2:1 ratio. Equations to predict total Hbmass (tHbmass) and Hbmass adjusted to fat-free mass (rHbmass) were developed with multivariable linear regression. The models were stratified for five complexity levels with the inclusion of anthropometric, biochemical, and fitness indices. Models for tHbmass (R2 = 0.87-0.92; root-mean-square error [RMSE] = 60.6-76.5 g) outperform the models for rHbmass (R2 = 0.28-0.58; RMSE = 1.00-1.26 g·kg-1). During internal validation, 9 of 10 of equations accurately predicted tHbmass (0.11 ± 54.7-54.8 ± 45.5 g; p = 0.18-0.99) and only 1 model differed significantly (p = 0.03). There were also no significant differences between observed and predicted values in 8 of 10 of equations for rHbmass (0.1 ± 1.4-1.0 ± 0.1 g·kg-1; p = 0.07-0.65) and 2 models showed significant differences (p = 0.01-0.04). Models present moderate-to-high accuracy. Equations are precise enough to provide complementary data in the epidemiology of diseases with abnormal hemoglobin values, antidoping policy or talent identification. However, they should not substitute direct testing of Hbmass in EA.
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Affiliation(s)
- Przemysław Kasiak
- 3rd Department of Internal Medicine and Cardiology, Medical University of Warsaw, Warsaw, Poland
| | - Tomasz Kowalski
- Department of Physiology, Institute of Sport-National Research Institute, Warsaw, Poland
| | - Raphaël Faiss
- Institute of Sports Sciences, Department of Physiology, Faculty of Biology and Medicine, University of Lausanne, Lausanne, Switzerland
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Wu Y, Shen J. Unraveling the intricacies of neutrophil extracellular traps in inflammatory bowel disease: Pathways, biomarkers, and promising therapies. Cytokine Growth Factor Rev 2024; 80:156-167. [PMID: 39438227 DOI: 10.1016/j.cytogfr.2024.10.003] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/21/2024] [Accepted: 10/06/2024] [Indexed: 10/25/2024]
Abstract
The development of inflammatory bowel disease (IBD), including ulcerative colitis and Crohn's disease, involves various factors and is characterized by persistent inflammation of the mucosal lining. However, the role of neutrophils in this process remains controversial. Neutrophil extracellular traps (NETs), which consist of chromatin, antimicrobial proteins, and oxidative enzymes, are released by neutrophils to trap pathogens. They are also involved in various immune-mediated and vascular diseases. NETs act as a vital defense mechanisms at the gut-mucosal interface and are frequently exposed to bacterial, viral, and fungal threats. However, they can also contribute to inflammation and worsen imbalances in the gut bacteria. Recent studies have suggested that NETs have a significant impact on IBD development. Previous studies have shown increased levels of NETs in tissue and blood samples from patients with IBD, as well as in experimental colitis mouse models. Therefore, this review discusses how NETs are formed and their role in the pathophysiology of IBD. It discusses how NETs may lead to tissue damage and contribute to IBD-associated complications. Moreover, non-invasive biomarkers are needed to replace invasive procedures such as endoscopy to better evaluate the disease status. Given the crucial role of NETs in IBD progression, this review focuses on potential NET biomarkers that can help predict the evolution of IBD. Furthermore, this review identifies potential therapeutic targets for regulating NET production, which could expand the range of available treatment options for IBD.
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Affiliation(s)
- Yilin Wu
- Division of Gastroenterology and Hepatology, Baoshan Branch, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China; Division of Gastroenterology and Hepatology, Key Laboratory of Gastroenterology and Hepatology, Ministry of Health, Inflammatory Bowel Disease Research Center, Shanghai 200127, China; Renji Hospital, School of Medicine, Shanghai Jiao Tong University, China; Shanghai Institute of Digestive Disease, No.160 PuJian Road, China
| | - Jun Shen
- Division of Gastroenterology and Hepatology, Baoshan Branch, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China; Division of Gastroenterology and Hepatology, Key Laboratory of Gastroenterology and Hepatology, Ministry of Health, Inflammatory Bowel Disease Research Center, Shanghai 200127, China; Renji Hospital, School of Medicine, Shanghai Jiao Tong University, China; Shanghai Institute of Digestive Disease, No.160 PuJian Road, China.
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Farrag K, Aksan A, Ademaj-Kospiri V, Leventi E, Stein J. Use of Biomarkers of Inflammation in the Differentiation of Iron Deficiency and Anaemia-Lessons from Inflammatory Bowel Disease. Diagnostics (Basel) 2024; 14:1515. [PMID: 39061651 PMCID: PMC11275494 DOI: 10.3390/diagnostics14141515] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 05/27/2024] [Revised: 07/05/2024] [Accepted: 07/09/2024] [Indexed: 07/28/2024] Open
Abstract
Iron deficiency and iron deficiency anaemia are common in inflammatory bowel disease (IBD), to the detriment of the patients' quality of life. Since ferritin, as an acute-phase protein (APP), has limited diagnostic value in IBD, concurrent assessment of C-reactive protein (CRP) is recommended. The World Health Organization suggests using α1-acid glycoprotein (AGP) as an additional biomarker due to its differing half-life. This study aimed to evaluate ferritin levels in patients with IBD using CRP and AGP, individually and in combination. A total of 118 patients with IBD (mean age: 45.48 ± 15.25 years, 47.46% female) were recruited, including 38 with Crohn's disease, 47 with ulcerative colitis, and 33 controls. The results showed that while CRP alone detected an inflammatory increase in ferritin of 29.76%, this increased to 82.14% when AGP or both AGP and CRP were considered (p < 0.05). Elevated AGP levels were more prevalent in patients with ulcerative colitis. However, concordance between high CRP and AGP levels was confirmed in only 55% of cases. Correcting for inflammation using CRP and/or AGP significantly improved the diagnostic accuracy of ferritin levels in patients with IBD, highlighting the challenge posed by inflammation when assessing iron deficiency.
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Affiliation(s)
- Karima Farrag
- Innere Medizin, DGD Kliniken Sachsenhausen, Schulstrasse 31, 60594 Frankfurt am Main, Germany;
- Interdisciplinary Crohn Colitis Centre Rhein-Main, Schifferstr. 59, 60594 Frankfurt am Main, Germany; (A.A.); (V.A.-K.); (E.L.)
| | - Aysegül Aksan
- Interdisciplinary Crohn Colitis Centre Rhein-Main, Schifferstr. 59, 60594 Frankfurt am Main, Germany; (A.A.); (V.A.-K.); (E.L.)
- Institute of Nutritional Science, Justus Liebig University, 35392 Giessen, Germany
| | - Valëza Ademaj-Kospiri
- Interdisciplinary Crohn Colitis Centre Rhein-Main, Schifferstr. 59, 60594 Frankfurt am Main, Germany; (A.A.); (V.A.-K.); (E.L.)
- Radiologie, Klinikum Aschaffenburg-Alzenau, Am Hasenkopf 1, 63739 Aschaffenburg, Germany
| | - Eleni Leventi
- Interdisciplinary Crohn Colitis Centre Rhein-Main, Schifferstr. 59, 60594 Frankfurt am Main, Germany; (A.A.); (V.A.-K.); (E.L.)
| | - Jürgen Stein
- Innere Medizin, DGD Kliniken Sachsenhausen, Schulstrasse 31, 60594 Frankfurt am Main, Germany;
- Interdisciplinary Crohn Colitis Centre Rhein-Main, Schifferstr. 59, 60594 Frankfurt am Main, Germany; (A.A.); (V.A.-K.); (E.L.)
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Gronnier M, Hedhli K, Sauzay C, Salle V, Duhaut P, Schmidt J, Dernoncourt A. Relevance of blood tumor markers in inpatients with significant involuntary weight loss and elevated levels of inflammation biomarkers. BMC Cancer 2024; 24:468. [PMID: 38622530 PMCID: PMC11017702 DOI: 10.1186/s12885-024-12201-0] [Citation(s) in RCA: 2] [Impact Index Per Article: 2.0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 06/14/2023] [Accepted: 03/28/2024] [Indexed: 04/17/2024] Open
Abstract
PURPOSE To assess the diagnostic performance of a panel of standard tumor markers (TMs) in patients hospitalized with significant involuntary weight loss (IWL) and elevated levels of inflammation biomarkers, and a combination of the TM panel and the finding of the computed tomography (CT) scan. METHODS We conducted a retrospective study in the internal medicine department at Amiens-Picardie University Medical Center (Amiens, France) between January 1st, 2015, and November 1st, 2021. The inclusion criteria were age 18 or over, significant IWL (≥ 5 kg over 6 months), elevated inflammation biomarkers (e.g. C-reactive protein), and assay data on two or more standard TMs (carcinoembryonic antigen (CEA), carbohydrate antigen (CA) 19 - 9, CA 15 - 3, CA 125, neuron-specific enolase (NSE), alpha-fetoprotein (AFP), calcitonin, and prostate-specific antigen (PSA)). The result of each TM assay was interpreted qualitatively (as positive or negative), according to our central laboratory's usual thresholds. RESULTS Cancer was diagnosed in 50 (37.0%) of the 135 patients included. Positivity for one or more TMs had a positive predictive value (PPV) of 0.55 [0.43-0.66], and a negative predictive value (NPV) of 0.84 [0.75-0.93] for cancer diagnosis. When combined with the presence of suspicious CT findings (e.g. a mass, enlarged lymph nodes and/or effusion), positivity for one or more TMs had a PPV of 0.92 [0.08-0.30]. In the absence of suspicious CT findings, a fully negative TM panel had an NPV of 0.96 [0.89-1.00]. CONCLUSION A negative TM panel argues against the presence of a cancer, especially in the absence of suspicious CT findings.
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Affiliation(s)
- Morgane Gronnier
- Department of Internal Medicine, Amiens-Picardie University Medical Center, Rue du Professeur Christian Cabrol, F-80054, Amiens, France
| | - Kaies Hedhli
- Laboratory of Hematology, Center of Human Biology, Amiens-Picardie University Medical Center, F-80054, Amiens, France
| | - Chloé Sauzay
- Laboratory of Biochemistry, Center of Human Biology, Amiens-Picardie University Medical Center, F-80054, Amiens, France
| | - Valéry Salle
- Department of Internal Medicine, Amiens-Picardie University Medical Center, Rue du Professeur Christian Cabrol, F-80054, Amiens, France
- RECIF, Amiens-Picardie University Medical Center, F-80000, Amiens, France
| | - Pierre Duhaut
- Department of Internal Medicine, Amiens-Picardie University Medical Center, Rue du Professeur Christian Cabrol, F-80054, Amiens, France
- RECIF, Amiens-Picardie University Medical Center, F-80000, Amiens, France
| | - Jean Schmidt
- Department of Internal Medicine, Amiens-Picardie University Medical Center, Rue du Professeur Christian Cabrol, F-80054, Amiens, France
- RECIF, Amiens-Picardie University Medical Center, F-80000, Amiens, France
| | - Amandine Dernoncourt
- Department of Internal Medicine, Amiens-Picardie University Medical Center, Rue du Professeur Christian Cabrol, F-80054, Amiens, France.
- RECIF, Amiens-Picardie University Medical Center, F-80000, Amiens, France.
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Cennamo M, Giuliano L, Arrigoni G, Fardone V, Russo R, De Tomasi LM, Bertani F, Cammarota G, Brunetti G, Del Vecchio L, Partenope M. Method Comparison of Erythrocyte Sedimentation Rate Automated Systems, the VES-MATIC 5 (DIESSE) and Test 1 (ALIFAX), with the Reference Method in Routine Practice. J Clin Med 2024; 13:847. [PMID: 38337540 PMCID: PMC10856312 DOI: 10.3390/jcm13030847] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 12/11/2023] [Revised: 01/26/2024] [Accepted: 01/30/2024] [Indexed: 02/12/2024] Open
Abstract
Background: The erythrocyte sedimentation rate (ESR) is a routine and aspecific test that is still widely used. The reference-manual method for ESR determination is the Westergren method. The VES-MATIC 5 is a novel, fully automated, and closed system based on a modified Westergren method. This study conceived the aim of comparing two ESR analytical analysers, Test 1 and the VES-MATIC 5, with the reference method in routine practice. Methods: This study included 264 randomly analysed samples. A comparison between the two methods and Westergren was performed, and they were evaluated for inter-run and intra-run precision. In addition, we investigated possible interferences and different sensitivities to conventional analytes. Results: The comparison of methods by Passing-Bablok analysis provided a good agreement for both systems, with a better correlation for VES-MATIC 5 (p = 0.96) than Test 1 (p = 0.93), and sensitivity studies did not show any significant influence. Conclusions: The VES-MATIC 5 analyser demonstrated excellent comparability with the reference method, and it had better performance than Test 1. It can be employed in routine practice, bringing advantages such as a reduction in the probability of human error compared to the manual method, as well as an increase in operator safety and environmental protection.
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Affiliation(s)
- Michele Cennamo
- Department of Translational Medical Sciences, University of Naples “Federico II”, 80126 Naples, Italy
- Clinical Pathology and Microbiology Unit, Laboratory Analysis, ASST Lariana, Hospital Sant’Anna, 22100 Como, Italy; (L.G.); (G.A.); (V.F.); (R.R.); (L.M.D.T.); (F.B.); (M.P.)
| | - Loredana Giuliano
- Clinical Pathology and Microbiology Unit, Laboratory Analysis, ASST Lariana, Hospital Sant’Anna, 22100 Como, Italy; (L.G.); (G.A.); (V.F.); (R.R.); (L.M.D.T.); (F.B.); (M.P.)
| | - Gloria Arrigoni
- Clinical Pathology and Microbiology Unit, Laboratory Analysis, ASST Lariana, Hospital Sant’Anna, 22100 Como, Italy; (L.G.); (G.A.); (V.F.); (R.R.); (L.M.D.T.); (F.B.); (M.P.)
| | - Valentina Fardone
- Clinical Pathology and Microbiology Unit, Laboratory Analysis, ASST Lariana, Hospital Sant’Anna, 22100 Como, Italy; (L.G.); (G.A.); (V.F.); (R.R.); (L.M.D.T.); (F.B.); (M.P.)
| | - Roberta Russo
- Clinical Pathology and Microbiology Unit, Laboratory Analysis, ASST Lariana, Hospital Sant’Anna, 22100 Como, Italy; (L.G.); (G.A.); (V.F.); (R.R.); (L.M.D.T.); (F.B.); (M.P.)
| | - Luca Maria De Tomasi
- Clinical Pathology and Microbiology Unit, Laboratory Analysis, ASST Lariana, Hospital Sant’Anna, 22100 Como, Italy; (L.G.); (G.A.); (V.F.); (R.R.); (L.M.D.T.); (F.B.); (M.P.)
| | - Fabio Bertani
- Clinical Pathology and Microbiology Unit, Laboratory Analysis, ASST Lariana, Hospital Sant’Anna, 22100 Como, Italy; (L.G.); (G.A.); (V.F.); (R.R.); (L.M.D.T.); (F.B.); (M.P.)
| | - Gaetano Cammarota
- Department of Diagnostic Innovation, Fondazione IRCCS Istituto Nazionale dei Tumori, 20133 Milan, Italy;
| | - Giovanni Brunetti
- Department of Diagnostics and Public Health, Section of Clinical Biochemistry, University of Verona, 37134 Verona, Italy;
| | - Lucia Del Vecchio
- Department of Nephrology and Dialysis, ASST Lariana, Hospital Sant’Anna, 22100 Como, Italy;
| | - Michelarcangelo Partenope
- Clinical Pathology and Microbiology Unit, Laboratory Analysis, ASST Lariana, Hospital Sant’Anna, 22100 Como, Italy; (L.G.); (G.A.); (V.F.); (R.R.); (L.M.D.T.); (F.B.); (M.P.)
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Azeez Z, Vallabhaneni P. It is all blue! Arch Dis Child Educ Pract Ed 2023; 108:150-151. [PMID: 35772941 DOI: 10.1136/archdischild-2021-323520] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Key Words] [Track Full Text] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 11/11/2021] [Accepted: 06/21/2022] [Indexed: 11/03/2022]
Affiliation(s)
- Zak Azeez
- Child Health, Swansea Bay University Health Board, Port Talbot, UK
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A rapid and accurate method for estimating the erythrocyte sedimentation rate using a hematocrit-corrected optical aggregation index. PLoS One 2022; 17:e0270977. [PMID: 35819942 PMCID: PMC9275726 DOI: 10.1371/journal.pone.0270977] [Citation(s) in RCA: 7] [Impact Index Per Article: 2.3] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 11/27/2021] [Accepted: 06/21/2022] [Indexed: 11/28/2022] Open
Abstract
Although both the erythrocyte sedimentation rate (ESR) and optically measured erythrocyte aggregation parameters are affected by the hematocrit, this interaction is not considered by the method used to estimate ESR that considers aggregation parameters. In this study, we investigated the relationship between the ESR obtained by the Westergren method and that obtained with an aggregation parameter, namely, the aggregation index (AI) of multiple hematocrit values and fibrinogen-spiked samples with an analysis time of 5–60 s, and attempted to develop a rapid and accurate ESR estimation method. The AIs obtained from 5- and 10-s optical measurements with a fixed hematocrit were highly correlated with the erythrocyte sedimentation velocity. Furthermore, the rate of the AI increase with an increasing hematocrit was not significantly affected by the fibrinogen concentration at these measurement times. On the basis of these results, we defined the hematocrit-corrected aggregation index (HAI). The exponential function of the HAI obtained from the 5-s measurement agreed well with the sedimentation velocity calculated to eliminate the effect of hindered settling, and the HAI and hematocrit could be used to calculate the time constant of the sedimentation curve with a linear regression equation. The ESR value at 1 h was calculated based on the modified Stokes’ law and the HAI obtained from the 5-s measurement and showed an excellent correlation (R = 0.966) with the ESR value obtained by the Westergren method over a wide range of hematocrit and fibrinogen concentrations.
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Relationship between survival and erythrocyte sedimentation rate in patients operated for lung cancer. TURKISH JOURNAL OF THORACIC AND CARDIOVASCULAR SURGERY 2022; 30:381-388. [PMID: 36303686 PMCID: PMC9580278 DOI: 10.5606/tgkdc.dergisi.2022.21217] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 12/04/2020] [Accepted: 01/24/2021] [Indexed: 11/21/2022]
Abstract
Background
This study aims to investigate the relationship between preoperative erythrocyte sedimentation rate and survival in patients undergoing pulmonary resection due to lung cancer.
Methods
Between January 2011 and July 2017, a total of 575 patients (433 males, 142 females; mean age: 61.2±9.9 years; range, 29 to 82 years) who were operated due to primary lung cancer in our clinic were retrospectively analyzed. The patients were grouped according to erythrocyte sedimentation rate to analyze the relationship between erythrocyte sedimentation rate and survival.
Results
The mean overall survival time was 61.8±1.7 months in 393 patients with an erythrocyte sedimentation rate of ≤24 mm/h and 48.9±2.9 months in 182 patients with an erythrocyte sedimentation rate of ≥25 mm/h (p<0.001). Among the patients with Stage 1-2 disease, the mean survival time was 66.2±1.9 in patients with an erythrocyte sedimentation rate of ≤24 mm/h and 53.8±3.2 in patients with an erythrocyte sedimentation rate of ≥25 mm/h (p=0.008). The mean survival time in patients with adenocarcinoma was 62.4±2.4 months in patients with ≤24 mm/h erythrocyte sedimentation rate and 46.1±4.6 months in patients with ≥25 mm/h erythrocyte sedimentation rate (p=0.003).
Conclusion
The relationship between elevated erythrocyte sedimentation rate and poor prognosis in patients with the same stage of the disease is promising for the use of erythrocyte sedimentation rate as a prognostic marker.
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EXPLORING THE USE OF THE ERYTHROCYTE SEDIMENTATION RATE AS AN INFLAMMATORY MARKER FOR FREE-RANGING WILDLIFE: A CASE STUDY IN AFRICAN BUFFALO (SYNCERUS CAFFER). J Wildl Dis 2022; 58:298-308. [PMID: 35276000 DOI: 10.7589/jwd-d-21-00114] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 07/01/2021] [Accepted: 12/03/2021] [Indexed: 11/20/2022]
Abstract
Measuring inflammatory markers is critical to evaluating both recent infection status and overall human and animal health; however, there are relatively few techniques that do not require specialized equipment or personnel for detecting inflammation among wildlife. Such techniques are useful in that they help determine individual and population-level inflammatory status without the infrastructure and reagents that many more-specific assays require. One such technique, known as the erythrocyte sedimentation rate (ESR), is a measure of how quickly erythrocytes (red blood cells) settle in serum, with a faster rate indicating a general, underlying inflammatory process is occurring. The technique is simple, inexpensive, and can be performed in the field without specialized equipment. We took advantage of a population of African buffalo (Syncerus caffer), well studied from June 2014 to May 2017, to understand the utility of ESR in an important wildlife species. When ESR was compared with other markers of immunity in African buffalo, it correlated to known measures of inflammation. We found that a faster ESR was significantly positively correlated with increased total globulin levels and significantly negatively correlated with increased red blood cell count and albumin levels. We then evaluated if ESR correlated to the incidence of five respiratory pathogens and infection with two tick-borne pathogens in African buffalo. Our results suggest that elevated ESR is associated with the incidence of bovine viral diarrhea virus infection, parainfluenza virus, and Mannheimia haemolytica infections as well as concurrent Anaplasma marginale and Anaplasma centrale coinfection. These findings suggest that ESR is a useful field test as an inflammatory marker in individuals and herds, helping us better monitor overall health status in wild populations.
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12
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Zare A, Sabahi M, Safari H, Kiani A, Schmidt MH, Arjipour M. Spinal Surgery and Subsequent ESR and WBC Changes Pattern: A Single Center Prospective Study. Korean J Neurotrauma 2021; 17:136-147. [PMID: 34760824 PMCID: PMC8558019 DOI: 10.13004/kjnt.2021.17.e33] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 02/23/2021] [Revised: 09/22/2021] [Accepted: 10/07/2021] [Indexed: 11/15/2022] Open
Abstract
Objective Postoperative inflammation and infections are common complications of spinal surgery and have similar symptoms. However, postoperative infection may lead to a poor outcome and must be differentiated from postoperative inflammation. The objective of this study is determine the changing pattern of postoperative ESR and WBC counts, and investigate the effects of different variables. Methods A total of 61 patients who underwent spinal surgery were enrolled in this prospective study. The erythrocyte sedimentation rate (ESR) and white blood cell (WBC) counts were measured the day before surgery and on 1st, 3rd, 5th, 7th, and 14th postoperative days. Results WBC counts increased on the 1st postoperative day in comparison with the preoperative day (p<0.001), and they gradually decreased until the preoperative value was reached on the 14th postoperative day (p=0.14). The ESR also increased postoperatively, reaching a peak on the 5th postoperative day in comparison with the preoperative day (p<0.001) and gradually decreased thereafter. However, on the 14th postoperative day, the ESR was significantly greater than the preoperative value (p<0.001). In addition, a significant positive correlation was observed between ESR and age, duration of surgery, intraoperative blood loss, and duration of anesthesia. Conclusion WBC count continued to rise and was the highest on the 1st postoperative day, after which it gradually decreased and attained normal values on the 14th postoperative day, while the ESR increased on the 1st postoperative day, reached the highest level in patients with and without simultaneous instrumentation on 7th and 5th postoperative days, respectively, and gradually decreased.
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Affiliation(s)
- Akram Zare
- Department of Neurosurgery, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
| | - Mohammadmahdi Sabahi
- Neurosurgery Research Group (NRG), Student Research Committee, Hamadan University of Medical Sciences, Hamadan, Iran
- Brain and Spinal Cord Injury Research Center, Neuroscience Institute, Tehran University of Medical Sciences, Tehran, Iran
| | - Hosein Safari
- Department of Neurosurgery, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
| | - Arash Kiani
- Department of Neurosurgery, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
| | - Meic H. Schmidt
- Department of Neurosurgery, University of New Mexico, Albuquerque, NM, USA
- Department of Neurosurgery, Clinical Neurosciences Center, University of Utah, Salt Lake City, UT, USA
| | - Mahdi Arjipour
- Brain and Spinal Cord Injury Research Center, Neuroscience Institute, Tehran University of Medical Sciences, Tehran, Iran
- Department of Neurosurgery, Hamadan University of Medical Sciences, Hamadan, Iran
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13
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Jarlborg M, Gabay C. Systemic effects of IL-6 blockade in rheumatoid arthritis beyond the joints. Cytokine 2021; 149:155742. [PMID: 34688020 DOI: 10.1016/j.cyto.2021.155742] [Citation(s) in RCA: 31] [Impact Index Per Article: 7.8] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/05/2021] [Revised: 09/13/2021] [Accepted: 10/05/2021] [Indexed: 12/15/2022]
Abstract
Interleukin (IL)-6 is produced locally in response to an inflammatory stimulus, and is able to induce systemic manifestations at distance from the site of inflammation. Its unique signaling mechanism, including classical and trans-signaling pathways, leads to a major expansion in the number of cell types responding to IL-6. This pleiotropic cytokine is a key factor in the pathogenesis of rheumatoid arthritis (RA) and is involved in many extra-articular manifestations that accompany the disease. Thus, IL-6 blockade is associated with various biological effects beyond the joints. In this review, the systemic effects of IL-6 in RA comorbidities and the consequences of its blockade will be discussed, including anemia of chronic disease, cardiovascular risks, bone and muscle functions, and neuro-psychological manifestations.
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Affiliation(s)
- Matthias Jarlborg
- Division of Rheumatology, University Hospital of Geneva, and Department of Pathology and Immunology, University of Geneva School of Medicine, Geneva, Switzerland; VIB-UGent Center for Inflammation Research and Ghent University, Ghent, Belgium
| | - Cem Gabay
- Division of Rheumatology, University Hospital of Geneva, and Department of Pathology and Immunology, University of Geneva School of Medicine, Geneva, Switzerland.
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14
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Castano-Jaramillo LM, Yamazaki-Nakashimada MA, O'Farrill-Romanillos PM, Muzquiz Zermeño D, Scheffler Mendoza SC, Venegas Montoya E, García Campos JA, Sánchez-Sánchez LM, Gámez González LB, Ramírez López JM, Bustamante Ogando JC, Vásquez-Echeverri E, Medina Torres EA, Lopez-Herrera G, Blancas Galicia L, Berrón Ruiz L, Staines-Boone AT, Espinosa-Padilla SE, Segura Mendez NH, Lugo Reyes SO. COVID-19 in the Context of Inborn Errors of Immunity: a Case Series of 31 Patients from Mexico. J Clin Immunol 2021; 41:1463-1478. [PMID: 34114122 PMCID: PMC8191444 DOI: 10.1007/s10875-021-01077-5] [Citation(s) in RCA: 36] [Impact Index Per Article: 9.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/16/2021] [Accepted: 05/25/2021] [Indexed: 12/15/2022]
Abstract
INTRODUCTION Patients with inborn errors of immunity (IEI) have a compromised or inappropriate immune response. Although they might be considered a high-risk group for severe SARS-CoV-2 infection, the reported impact of COVID-19 in these patients has been reassuring, while the differential susceptibility of distinct types of IEI remains unclear. OBJECTIVE We aimed to describe the findings and outcomes of our known patients with IEI who were diagnosed with COVID-19. METHODS In a retrospective study from March 2020 to February 2021, four centers in Mexico collected clinical, laboratory, and genetic data from pediatric and adult patients with known diagnoses of IEI who presented with COVID-19, based on compatible symptoms and positive SARS-CoV-2 testing or known household exposure. RESULTS We report 31 patients with known IEI from Mexico who presented with SARS-CoV-2 infection. Seventy-four percent were male, 52% were pediatric, and 81% survived. Their ages ranged from 5 months to 56 years, with a median of 17 years. Sixty-five percent had predominant antibody deficiencies, 48% were hospitalized, and 26% required ICU. Pediatric patients had a higher hospital admission rate than adults. Inpatient mortality was 40%, and ICU mortality rate was 63%. Forty-eight percent developed pneumonia, while 36% had evidence of hyperinflammation (4 adults and 7 children). Predominant laboratory features were lymphopenia and thrombocytopenia, seen in 70 and 44% of patients, respectively. The serum D-dimer median value was 2.6 (0.5-20.6) μg/mL, and the median highest ferritin value was 1015 (32-10,303) ng/mL. Intravenous immunoglobulin was used in 80% of patients. Other treatments included macrolides (39%) and corticosteroids (29%). Six patients died from secondary infection or uncontrolled systemic inflammation. DISCUSSION Although impaired immunity due to IEI may be a predisposing factor for severe COVID-19, most of our patients with IEI who acquired the SARS-CoV-2 infection developed a well-tolerated infection and survived, as have more than 80% of worldwide reported patients to date. An impaired immune or inflammatory response may be a predisposing factor for some and a protective factor for others. A systematic review of the literature could help identify those patients at risk of severe disease and complications. Healthcare-associated infections should be aggressively prevented.
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Affiliation(s)
| | | | - Patricia M O'Farrill-Romanillos
- Allergy and Immunology Department, Hospital de Especialidades, National Medical Center, Siglo XXI" IMSS, Mexico City, Mexico
| | | | | | - Edna Venegas Montoya
- Immunology Service, Hospital de Especialidades UMAE 25 IMSS, Monterrey, NL, Mexico
| | | | | | - Luisa B Gámez González
- Clinical Immunology Service, Hospital Infantil Especialidades de Chihuahua, Chihuahua, Mexico
| | | | - Juan Carlos Bustamante Ogando
- Immune Deficiencies Lab., National Institute of Pediatrics, Av Iman 1, Torre de Investigacion Piso 9, Insurgentes Cuicuilco, Coyoacan, 04530, Mexico City, Mexico
| | - Estefanía Vásquez-Echeverri
- Immune Deficiencies Lab., National Institute of Pediatrics, Av Iman 1, Torre de Investigacion Piso 9, Insurgentes Cuicuilco, Coyoacan, 04530, Mexico City, Mexico
| | - Edgar Alejandro Medina Torres
- Immune Deficiencies Lab., National Institute of Pediatrics, Av Iman 1, Torre de Investigacion Piso 9, Insurgentes Cuicuilco, Coyoacan, 04530, Mexico City, Mexico
| | - Gabriela Lopez-Herrera
- Immune Deficiencies Lab., National Institute of Pediatrics, Av Iman 1, Torre de Investigacion Piso 9, Insurgentes Cuicuilco, Coyoacan, 04530, Mexico City, Mexico
| | - Lizbeth Blancas Galicia
- Immune Deficiencies Lab., National Institute of Pediatrics, Av Iman 1, Torre de Investigacion Piso 9, Insurgentes Cuicuilco, Coyoacan, 04530, Mexico City, Mexico
| | - Laura Berrón Ruiz
- Immune Deficiencies Lab., National Institute of Pediatrics, Av Iman 1, Torre de Investigacion Piso 9, Insurgentes Cuicuilco, Coyoacan, 04530, Mexico City, Mexico
| | | | - Sara Elva Espinosa-Padilla
- Immune Deficiencies Lab., National Institute of Pediatrics, Av Iman 1, Torre de Investigacion Piso 9, Insurgentes Cuicuilco, Coyoacan, 04530, Mexico City, Mexico
| | - Nora Hilda Segura Mendez
- Allergy and Immunology Department, Hospital de Especialidades, National Medical Center, Siglo XXI" IMSS, Mexico City, Mexico
| | - Saul O Lugo Reyes
- Immune Deficiencies Lab., National Institute of Pediatrics, Av Iman 1, Torre de Investigacion Piso 9, Insurgentes Cuicuilco, Coyoacan, 04530, Mexico City, Mexico.
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15
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Maki R, Tabe Y, Yamamoto T, Takemura H, Sawada T, Esaki T, Higuchi M, Misawa S, Miyake K, Miida T, Nagai Y, Ohsaka A. Accuracy study of a novel alternate method measuring erythrocyte sedimentation rate for prototype hematology analyzer Celltac α. Int J Lab Hematol 2021; 43:588-596. [PMID: 33929779 DOI: 10.1111/ijlh.13554] [Citation(s) in RCA: 2] [Impact Index Per Article: 0.5] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/30/2020] [Revised: 04/03/2021] [Accepted: 04/07/2021] [Indexed: 11/26/2022]
Abstract
INTRODUCTION The erythrocyte sedimentation rate (ESR) is a nonspecific inflammation indicator. In laboratory testing, automated ESR analyzers may use the reference Westergren method (Reference WG), modified Westergren (Modified WG), or Alternate ESR method (Alternate ESR) based on photometric rheology. A prototype hematology analyzer Celltac α+ (Nihon Kohden Corporation) with built-in Novel ESR analysis technology (Novel ESR) was developed to improve the accuracy of Alternate ESR. Alternate ESR uses only the aggregation phase information of Reference WG. The Novel ESR adds sedimentation and packing phase information obtained by hematology analyzer measurands. High correlation with WG was ensured by predicting the ESR value using Hematocrit (Hct) and MCV values as correcting parameters. METHODS Novel ESR was compared with Modified WG (MONITOR-40, Joko Corporation) and Reference WG, according to internationally recognized guidelines: Precision, carryover, limit of quantification, comparability, linearity, accuracy, and fibrinogen sensitivity. Samples from healthy volunteers and clinical patients were used. The correction performance of Novel ESR and Modified WG was compared with Reference WG by regression analysis in three range categories for ESR and measurands affecting ESR correction (Hct, MCV, and MCH). RESULTS Novel ESR showed sufficient basic performance and comparability with Modified WG. In the accuracy study comparing with Reference WG, the regression equation was y = 1.026x + 0.5(r = .945,P < .001;n = 271). When evaluating the correction performance, the slopes were within 0.8-1.2, except for the high part of Hct. All intercepts were within 10 mm. CONCLUSION This study validated the correction performance to the initial estimated ESR value by aggregation phase information using information reflecting sedimentation and packing phase obtained from automated hematology analyzer. The Celltac α+ Novel ESR provided results equivalent to Reference WG.
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Affiliation(s)
- Ryosuke Maki
- Clinical Laboratory Medicine, Juntendo University Urayasu Hospital, Chiba, Japan
| | - Yoko Tabe
- Clinical Laboratory Medicine, Juntendo University Graduate School of Medicine, Tokyo, Japan.,Next Generation Hematology Laboratory Medicine, Juntendo University Graduate School of Medicine, Tokyo, Japan
| | | | | | - Tomohiro Sawada
- Clinical Laboratory Medicine, Juntendo University Urayasu Hospital, Chiba, Japan
| | | | - Makoto Higuchi
- IVD Business Operations, Nihon Kohden Corp., Tokyo, Japan
| | - Shigeki Misawa
- Clinical Laboratory, Juntendo University Hospital, Tokyo, Japan
| | - Kazunori Miyake
- Clinical Laboratory Medicine, Juntendo University Urayasu Hospital, Chiba, Japan
| | - Takashi Miida
- Clinical Laboratory Medicine, Juntendo University Graduate School of Medicine, Tokyo, Japan.,Clinical Laboratory, Juntendo University Hospital, Tokyo, Japan
| | - Yutaka Nagai
- IVD Business Operations, Nihon Kohden Corp., Tokyo, Japan
| | - Akimichi Ohsaka
- Transfusion Medicine and Stem Cell Regulation, Juntendo University Graduate School of Medicine, Tokyo, Japan
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16
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Ştefanescu S, Cocoş R, Turcu-Stiolica A, Mahler B, Meca AD, Giura AMC, Bogdan M, Shelby ES, Zamfirescu G, Pisoschi CG. Evaluation of prognostic significance of hematological profiles after the intensive phase treatment in pulmonary tuberculosis patients from Romania. PLoS One 2021; 16:e0249301. [PMID: 33793598 PMCID: PMC8016233 DOI: 10.1371/journal.pone.0249301] [Citation(s) in RCA: 18] [Impact Index Per Article: 4.5] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 10/29/2020] [Accepted: 03/15/2021] [Indexed: 12/12/2022] Open
Abstract
We evaluated in this cohort study the predictive ability of 23 peripheral blood parameters and ratios for treatment outcomes after the 2-month intensive phase in patients with PTB. In 63 patients out of 90 that turned culture negative, a significant decrease in white blood cell count, neutrophils, monocyte, hemoglobin, platelet, plateletcrit, erythrocyte sedimentation rate, MLR, NLR, PLR and SII values after anti-TB therapy compared to pretreatment was observed (p <0.001). Logistic regression analysis generated a model of predictors consisting of nine covariates. Spearman's correlation analysis revealed significant positive correlations between NLR with NEU (r = 0.79, p<0.01), SII with NEU (r = 0.846, p<0.01), PLT with SII (r = 0.831, p<0.01), PLT with PCT (r = 0.71, p<0.01) and MPV with P-LCR (r = 0,897, p<0.01) in 63 patients out of 90 that turned culture negative after 2 months of treatment. ROC curve analysis indicated that all areas under the curve (AUC) revealed no statistically significant results, except lymphocyte for culture conversion. In summary, here we observed a set of hematological parameters that declined significantly as the disease was treated in patients that turned culture negative. Despite some limitations, our findings are useful for further studies aiming to identify hematological profiles that could predict the treatment outcome.
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Affiliation(s)
- Simona Ştefanescu
- Clinical Analysis Laboratory, Clinical Emergency County Hospital Craiova, Craiova, Romania
| | - Relu Cocoş
- Department of Medical Genetics, University of Medicine and Pharmacy “Carol Davila”, Bucharest, Romania
- Institute of Pneumophtisiology “Marius Nasta”, Bucharest, Romania
| | - Adina Turcu-Stiolica
- Department of Pharmacoeconomics, University of Medicine and Pharmacy of Craiova, Craiova, Romania
| | - Beatrice Mahler
- Institute of Pneumophtisiology “Marius Nasta”, Bucharest, Romania
- Pneumology Department (II), University of Medicine and Pharmacy “Carol Davila”, Bucharest, Romania
| | - Andreea-Daniela Meca
- Department of Pharmacology, University of Medicine and Pharmacy of Craiova, Craiova, Romania
| | - Ana Maria Cristina Giura
- Department of Preventive Dentistry, University of Medicine and Pharmacy “Carol Davila”, Bucharest, Romania
| | - Maria Bogdan
- Department of Pharmacology, University of Medicine and Pharmacy of Craiova, Craiova, Romania
| | - Elena-Silvia Shelby
- Scientific Research Nucleus, Dr. Nicolae Robanescu National Clinical Centre for Children’s Neurorecovery, Bucharest, Romania
| | - Georgeta Zamfirescu
- Clinical Analysis Laboratory, Leamna Pneumophtisiology Hospital, Craiova, Romania
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17
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Chauffaille MDL, Takihi IY, Prieto WH, Russo PDST, Sandes AF, Perazzio AB, Silva MCA, Gonçalves MV. New reference values for the old erythrocyte sedimentation rate. Int J Lab Hematol 2021; 43:O214-O217. [PMID: 33794065 DOI: 10.1111/ijlh.13523] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.3] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/22/2020] [Revised: 03/03/2021] [Accepted: 03/08/2021] [Indexed: 11/27/2022]
Affiliation(s)
| | | | | | | | - Alex F Sandes
- Division of Hematology, Fleury Group, São Paulo, Brazil
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18
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Orkmez M, Orhan S, Bozdayi MA, Tarakcioglu M. Comparison of the StaRRsed Interliner device with Westergren method in erythrocyte sedimentation rate measurement. Int J Lab Hematol 2021; 43:616-622. [PMID: 33522096 DOI: 10.1111/ijlh.13444] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 05/12/2020] [Revised: 10/27/2020] [Accepted: 11/28/2020] [Indexed: 11/30/2022]
Abstract
INTRODUCTION With recent advances in technology, many manual tests are being replaced by automated devices due to a wide range of advantages. One of these tests is the erythrocyte sedimentation rate (ESR) test that is used to determine inflammatory activity. This study aimed to evaluate the agreement between the Starrsed Interliner sedimentation device and the gold standard method, that is the Westergren method, used in ESR measurement. METHODS One hundred fifty-one patients who presented to Gaziantep University Faculty of Medicine, Şahinbey Training and Research Hospital were included in this study. ESR values were measured simultaneously within 2 hours using the ESR analyzer Starrsed Interliner device and the gold standard method of measuring ESR, that is the Westergren method, from blood samples collected from the same patients in EDTA and citrate tubes. RESULTS Agreement between the results from the Starrsed Interliner device and the Westergren method was evaluated using the Intraclass Correlation method. Consequently, a poor correlation was observed at values <20 mm/h, a moderate correlation was observed at values 20 to 80 mm/h and >80 mm/h, and an excellent correlation was observed when all results were considered. Method comparison was conducted according to the Passing-Bablok regression analysis (y = -1.50 + 0.75x) (P < .0001). The mean difference between the two methods was 10.1 according to the Bland-Altman analysis. CONCLUSIONS Despite the advantages of the Starrsed Interliner device, such as lower laboratory workloads, lower costs and turnaround time, the difference between the two methods, as found in this study, may lead to different clinical interpretations for results in some patient.
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Affiliation(s)
- Mustafa Orkmez
- Department of Medical Biochemistry, Faculty of Medicine, Gaziantep University, Gaziantep, Turkey
| | - Seren Orhan
- Department of Medical Biochemistry, Faculty of Medicine, Gaziantep University, Gaziantep, Turkey
| | - Mehmet Akif Bozdayi
- Department of Medical Biochemistry, Faculty of Medicine, Gaziantep University, Gaziantep, Turkey
| | - Mehmet Tarakcioglu
- Department of Medical Biochemistry, Faculty of Medicine, Gaziantep University, Gaziantep, Turkey
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19
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Kumar S, Ram R, Sarkar A, DasGupta S, Chakraborty S. Rapid determination of erythrocyte sedimentation rate (ESR) by an electrically driven blood droplet biosensor. BIOMICROFLUIDICS 2020; 14:064108. [PMID: 33312329 PMCID: PMC7710385 DOI: 10.1063/5.0026332] [Citation(s) in RCA: 4] [Impact Index Per Article: 0.8] [Reference Citation Analysis] [Abstract] [Track Full Text] [Subscribe] [Scholar Register] [Received: 08/23/2020] [Accepted: 11/13/2020] [Indexed: 05/10/2023]
Abstract
In healthcare practice, the sedimentation rate of red blood cells (erythrocytes) is a widely used clinical parameter for screening of several ailments such as stroke, infectious diseases, and malignancy. In a traditional pathological setting, the total time taken for evaluating this parameter varies typically from 1 to 2 h. Furthermore, the volume of human blood to be drawn for each test, following a gold standard laboratory technique (alternatively known as the Westergren method), varies from 4 to 5 ml. Circumventing the above constraints, here we propose a rapid (∼1 min) and highly energy efficient method for the simultaneous determination of hematocrit and erythrocyte sedimentation rate (ESR) on a microfluidic chip, deploying electrically driven spreading of a tiny drop of blood sample (∼8 μl). Our unique approach estimates these parameters by correlating the same with the time taken by the droplet to spread over a given radius, reproducing the results from more elaborate laboratory settings to a satisfactory extent. Our novel methodology is equally applicable for determining higher ranges of ESR such as high concentration of bilirubin and samples corresponding to patients with anemia and patients with some severe inflammation. Furthermore, the minimal fabrication steps involved in the process, along with the rapidity and inexpensiveness of the test, render the suitability of the strategy in extreme point-of-care settings.
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Affiliation(s)
- Sumit Kumar
- Advanced Technology Development Centre, Indian Institute of Technology Kharagpur, Kharagpur, West Bengal 721302, India
| | - Rishi Ram
- Department of Mechanical Engineering, Indian Institute of Technology (BHU), Varanasi, Uttar Pradesh 221005, India
| | | | | | - Suman Chakraborty
- Author to whom correspondence should be addressed:. Telephone: +913222282990. Fax: +913222282278
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20
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Edward SS, Akande JI, Obiajunwa PO. Rifampicin-Resistant Tuberculosis in a Toddler: A Report of a Rare Paediatric Case in Obafemi Awolowo University Teaching Hospitals Complex, Ile-Ife, Nigeria. Niger Med J 2020; 61:281-283. [PMID: 33487854 PMCID: PMC7808285 DOI: 10.4103/nmj.nmj_210_20] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 06/09/2020] [Revised: 06/16/2020] [Accepted: 07/20/2020] [Indexed: 11/04/2022] Open
Abstract
The emergence of resistant strains of mycobacterium tuberculosis (TB) to antituberculous drugs has compounded the management of the chronic infection. More than 90% of rifampicin (RIF)-resistant isolates are also isoniazid resistant; hence, rifampicin resistance (RR) is a surrogate marker for multidrug resistant TB (MDR-TB). Although there are limited reports of pediatric RR/MDR-TB in Nigeria, there had not been similar report in our hospital until now. A 2-year-old girl was admitted with 2-month history of fever, cough with dyspnea, and progressive weight loss. There was no known contact with adult who had chronic cough; the toddler and her parents have not been treated for TB in the past. Her chest X-ray showed nodular opacities, while gastric washout for GeneXpert MTB/RIF confirmed RIF-resistant TB. The parents declined screening for TB despite counseling. The patient was subsequently referred to a specialized center for the management of drug-resistant TB, but the parents failed to go for the treatment. Young children are at risk of developing TB disease and MDR/RR-TB, which is more complex to manage than drug-susceptible TB due to longer treatment duration, increased toxicity, as well as poor parental compliance to the demand of treatment.
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Affiliation(s)
- S S Edward
- Department of Paediatrics, Paediatric Gastroenterology/Nutrition Unit, Obafemi Awolowo University Teaching Hospitals Complex, Ile-Ife, Nigeria
| | - J I Akande
- Department of Paediatrics, Paediatric Gastroenterology/Nutrition Unit, Obafemi Awolowo University Teaching Hospitals Complex, Ile-Ife, Nigeria
| | - P O Obiajunwa
- Department of Paediatrics, Paediatric Pulmonology Unit, Obafemi Awolowo University Teaching Hospitals Complex, Ile-Ife, Nigeria
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21
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Balasundaram PK, Kanakamma LG, Jayageetha K, Selvarajan B. Epidemiological, clinical and laboratory features of leptospirosis compared to other acute febrile illnesses. J R Coll Physicians Edinb 2020; 50:118-123. [PMID: 32568280 DOI: 10.4997/jrcpe.2020.208] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.2] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 01/16/2023] Open
Abstract
BACKGROUND Background Leptospirosis is a common zoonotic disease. Many waterborne diseases and mosquito-borne diseases are common causes of acute febrile illnesses in the southern Indian state of Kerala posing diagnostic challenges. The objective of this study was to describe the epidemiological, clinical and laboratory features of leptospirosis diagnosed using the modified Faine's criteria (with amendment) compared to other common acute febrile illnesses. METHODS In this prospective study, all consecutive patients with acute febrile illness, headache and myalgia presenting to our tertiary care hospital's single unit from March 2013 to February 2015 were subjected to detailed history taking and thorough clinical examination. Leptospiral immunoglobulin M (IgM) serology was confirmed by RecombiLISA ELISA and modified Faine's criteria were used for diagnosis of leptospirosis. Other diagnoses for acute febrile illnesses were assigned based on clinical and laboratory investigations. Bivariate and regression analysis was carried out to analyse epidemiological, clinical and laboratory parameters of both groups. RESULTS A total of 389 patients were enrolled, out of which 110 patients had the presumptive diagnosis of leptospirosis. Among the 279 with non-leptospirosis acute febrile illness, dengue (39%) and other viral febrile illnesses (25%) were the most common diagnoses. Regression analysis identified several epidemiological (contact with contaminated animals or water and drinking unboiled water), clinical (conjunctival congestion and muscle tenderness) and laboratory investigations (leucocytosis, neutrophilia, elevated erythrocyte sedimentation rate [ESR] and aspartate aminotransferase) which were helpful in distinguishing leptospirosis from other acute febrile illnesses. CONCLUSION Our study suggests that certain epidemiological, clinical and laboratory features in patients with leptospirosis may allow an early diagnosis. Our study also underscores the usefulness of confirming the leptospiral serology by enzyme-linked immunosorbent assay (ELISA) in combination with relevant epidemiological and clinical features in diagnosing leptospirosis using the modified Faine's criteria.
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Affiliation(s)
| | | | - Kumari Jayageetha
- Department of Community Medicine, Government Medical College, Thiruvananthapuram, Kerala, India
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Felaco P, Felaco M, Franceschelli S, Ferrone A, Gatta DMP, Speranza L, Patruno A, De Lutiis MA, Ballerini P, Sirolli V, Grilli A, Bonomini M, Pesce M. Erythropoietin induces miRNA-210 by JAK2/STAT5 signaling in PBMCs of End-stage Renal Disease patients. FEBS J 2020; 287:5167-5182. [PMID: 32196922 DOI: 10.1111/febs.15302] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.2] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/17/2019] [Revised: 02/03/2020] [Accepted: 03/17/2020] [Indexed: 12/13/2022]
Abstract
Anemia of chronic kidney disease is associated with blunted response/resistance to erythropoietin-stimulating agents (ESAs) in hemodialysis (HD) patients. Several molecules have been successfully associated with ESA responsiveness; however, none of them is now considered a valid therapeutic biomarker of erythropoietin resistance in these patients. We performed an evaluation of the level of specific plasma circulating miRNAs in blood samples of HD patients, in relation to ESA treatment, with a follow-up of 1 year (T0-T3). We found significantly lower circulating levels of all miRNAs analyzed at baseline (T0) in HD patients vs. healthy control (HC). The plasmatic levels of miRNA-210 resulted significantly and negatively associated with Erythropoietin Resistance Index (ERI), and the variance of ΔmiRNA-210 (miRNA-210T3 minus miRNA-210T0 ) explained significant percentage of ΔERI (ERIT3 minus ERIT0 ) variance. The receiver operating characteristic analysis at T0 showed that the plasmatic level of miRNA-210 could distinguish HD patients with positive or negative trend in ERI at T3. In vitro, recombinant human erythropoietin (EPO) induced significant release of miRNA-210 from cultured peripheral blood mononuclear cells, through the activation of Janus kinase 2 (JAK2)/ signal transducer and activator of transcription 5 (STAT5) signaling, but not by the activation of the MAPK protein 38α and extracellular signal-regulated kinase ½. Accordingly, HD patients with negative ΔERI showed higher level of phosphor-Janus kinase 2 and nuclear translocation of phosphor-signal transducer and activator of transcription 5. vs. patients with positive ΔERI or HC. Our data highlighted that chronic HD significantly reduces the circulating level of the miRNAs evaluated; within the targets analyzed, the miRNA-210 could be considered as a prognostic indicator of ESA responsiveness and index for anemia management.
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Affiliation(s)
- Paolo Felaco
- Department of Medicine and Aging Sciences, Medicine and Health Science School, University G. d'Annunzio, Chieti, Italy
| | - Mario Felaco
- Department of Medicine and Aging Sciences, Medicine and Health Science School, University G. d'Annunzio, Chieti, Italy
| | - Sara Franceschelli
- Department of Psychological, Health and Territorial Sciences, Medicine and Health Science School, University G. d'Annunzio, Chieti, Italy
| | - Alessio Ferrone
- Department of Medicine and Aging Sciences, Medicine and Health Science School, University G. d'Annunzio, Chieti, Italy
| | - Daniela M P Gatta
- Department of Medicine and Aging Sciences, Medicine and Health Science School, University G. d'Annunzio, Chieti, Italy
| | - Lorenza Speranza
- Department of Medicine and Aging Sciences, Medicine and Health Science School, University G. d'Annunzio, Chieti, Italy
| | - Antonia Patruno
- Department of Medicine and Aging Sciences, Medicine and Health Science School, University G. d'Annunzio, Chieti, Italy
| | - Maria A De Lutiis
- Department of Medicine and Aging Sciences, Medicine and Health Science School, University G. d'Annunzio, Chieti, Italy
| | - Patrizia Ballerini
- Department Neurosciences, Imaging and Clinical Sciences, University G. d'Annunzio, Chieti, Italy
| | - Vittorio Sirolli
- Department of Medicine and Aging Sciences, Medicine and Health Science School, University G. d'Annunzio, Chieti, Italy
| | - Alfredo Grilli
- Department of Medical, Oral and Biotechnological Sciences, University G. d'Annunzio, Chieti, Italy
| | - Mario Bonomini
- Department of Medicine and Aging Sciences, Medicine and Health Science School, University G. d'Annunzio, Chieti, Italy
| | - Mirko Pesce
- Department of Medicine and Aging Sciences, Medicine and Health Science School, University G. d'Annunzio, Chieti, Italy
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Hysong AA, Posey SL, Blum DM, Benvenuti MA, Benvenuti TA, Johnson SR, An TJ, Devin JK, Obremskey WT, Martus JE, Moore-Lotridge SN, Schoenecker JG. Necrotizing Fasciitis: Pillaging the Acute Phase Response. J Bone Joint Surg Am 2020; 102:526-537. [PMID: 31977818 PMCID: PMC8590823 DOI: 10.2106/jbjs.19.00591] [Citation(s) in RCA: 20] [Impact Index Per Article: 4.0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Indexed: 02/01/2023]
Affiliation(s)
| | - Samuel L Posey
- Vanderbilt University School of Medicine, Nashville, Tennessee
| | - Deke M Blum
- Vanderbilt University School of Medicine, Nashville, Tennessee
| | - Michael A Benvenuti
- Department of Orthopaedics and Rehabilitation (M.A.B., T.A.B., S.R.J., T.J.A., W.T.O., J.E.M., S.N.M.-L., and J.G.S.), Division of Diabetes, Endocrinology, and Metabolism (J.K.D.), and Departments of Pediatrics (J.E.M and J.G.S.), Pathology, Microbiology, and Immunology (J.G.S.), and Pharmacology (J.G.S.), Vanderbilt University Medical Center, Nashville, Tennessee
| | - Teresa A Benvenuti
- Department of Orthopaedics and Rehabilitation (M.A.B., T.A.B., S.R.J., T.J.A., W.T.O., J.E.M., S.N.M.-L., and J.G.S.), Division of Diabetes, Endocrinology, and Metabolism (J.K.D.), and Departments of Pediatrics (J.E.M and J.G.S.), Pathology, Microbiology, and Immunology (J.G.S.), and Pharmacology (J.G.S.), Vanderbilt University Medical Center, Nashville, Tennessee
| | - Samuel R Johnson
- Department of Orthopaedics and Rehabilitation (M.A.B., T.A.B., S.R.J., T.J.A., W.T.O., J.E.M., S.N.M.-L., and J.G.S.), Division of Diabetes, Endocrinology, and Metabolism (J.K.D.), and Departments of Pediatrics (J.E.M and J.G.S.), Pathology, Microbiology, and Immunology (J.G.S.), and Pharmacology (J.G.S.), Vanderbilt University Medical Center, Nashville, Tennessee
| | - Thomas J An
- Department of Orthopaedics and Rehabilitation (M.A.B., T.A.B., S.R.J., T.J.A., W.T.O., J.E.M., S.N.M.-L., and J.G.S.), Division of Diabetes, Endocrinology, and Metabolism (J.K.D.), and Departments of Pediatrics (J.E.M and J.G.S.), Pathology, Microbiology, and Immunology (J.G.S.), and Pharmacology (J.G.S.), Vanderbilt University Medical Center, Nashville, Tennessee
| | - Jessica K Devin
- Department of Orthopaedics and Rehabilitation (M.A.B., T.A.B., S.R.J., T.J.A., W.T.O., J.E.M., S.N.M.-L., and J.G.S.), Division of Diabetes, Endocrinology, and Metabolism (J.K.D.), and Departments of Pediatrics (J.E.M and J.G.S.), Pathology, Microbiology, and Immunology (J.G.S.), and Pharmacology (J.G.S.), Vanderbilt University Medical Center, Nashville, Tennessee
| | - William T Obremskey
- Department of Orthopaedics and Rehabilitation (M.A.B., T.A.B., S.R.J., T.J.A., W.T.O., J.E.M., S.N.M.-L., and J.G.S.), Division of Diabetes, Endocrinology, and Metabolism (J.K.D.), and Departments of Pediatrics (J.E.M and J.G.S.), Pathology, Microbiology, and Immunology (J.G.S.), and Pharmacology (J.G.S.), Vanderbilt University Medical Center, Nashville, Tennessee
| | - Jeffrey E Martus
- Department of Orthopaedics and Rehabilitation (M.A.B., T.A.B., S.R.J., T.J.A., W.T.O., J.E.M., S.N.M.-L., and J.G.S.), Division of Diabetes, Endocrinology, and Metabolism (J.K.D.), and Departments of Pediatrics (J.E.M and J.G.S.), Pathology, Microbiology, and Immunology (J.G.S.), and Pharmacology (J.G.S.), Vanderbilt University Medical Center, Nashville, Tennessee
| | - Stephanie N Moore-Lotridge
- Department of Orthopaedics and Rehabilitation (M.A.B., T.A.B., S.R.J., T.J.A., W.T.O., J.E.M., S.N.M.-L., and J.G.S.), Division of Diabetes, Endocrinology, and Metabolism (J.K.D.), and Departments of Pediatrics (J.E.M and J.G.S.), Pathology, Microbiology, and Immunology (J.G.S.), and Pharmacology (J.G.S.), Vanderbilt University Medical Center, Nashville, Tennessee
| | - Jonathan G Schoenecker
- Department of Orthopaedics and Rehabilitation (M.A.B., T.A.B., S.R.J., T.J.A., W.T.O., J.E.M., S.N.M.-L., and J.G.S.), Division of Diabetes, Endocrinology, and Metabolism (J.K.D.), and Departments of Pediatrics (J.E.M and J.G.S.), Pathology, Microbiology, and Immunology (J.G.S.), and Pharmacology (J.G.S.), Vanderbilt University Medical Center, Nashville, Tennessee
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Ramadurai M, Rajendran G, Bama TS, Prabhu P, Kathiravan K. Biocompatible thiolate protected copper nanoclusters for an efficient imaging of lung cancer cells. JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY 2020; 205:111845. [PMID: 32172137 DOI: 10.1016/j.jphotobiol.2020.111845] [Citation(s) in RCA: 9] [Impact Index Per Article: 1.8] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Subscribe] [Scholar Register] [Received: 07/22/2019] [Revised: 02/14/2020] [Accepted: 03/02/2020] [Indexed: 12/18/2022]
Abstract
We report, the one-pot synthesis of water-soluble and biocompatible 3-mercaptopropylsulfonate (MPS) protected novel copper nanoclusters (CuNCs). Interestingly, the TEM image of MPS protected CuNCs exhibits an ultrasmall nanoclusters of particle size <2-nm, similar to its Au and Ag analogue. The hydrophilic and biocompability property of thiolate protected CuNCs. i.e., MPS stabilized CuNCs and its luminescent nature gave rise to maximum quantum yield of 1.5%. Further, as achieved CuNCs was investigated for haemocompatibility, cell viability and fluorescent microscopic analysis with A549 lung cancer cell line. Haemolytic study was examined using human RBCs in the concentration range of 4 to 22 μg/mL for which 7.5% of haemolysis was obtained for an optimum concentration of 22 μg/mL of CuNCs. The cell viability analysis was carried out by MTT assay using A549 lung cancer cells for the minimum (10 μg/mL) and maximum (45 μg/mL) concentration of CuNCs which reports 93.1% and 38.2% cell viability respectively. The inverted light microscopic images from the control and CuNCs treated (20 μg/mL) cells exhibited an excellent biocompatibility with a normal morphology. Upon increasing the concentration of CuNCs upto 45 μg/mL, the cell viability trends to decrease and the cell morphology also denature gradually. Further, the bio-imaging application of CuNCs was analyzed with A549 lung cancer cells. The efficient imaging with CuNCs treated (20 μg/mL) A549 cells resulted in a green colour emission using FITC filter (460- 490 nm). Thereby the obtained results confirm the applicability of CuNCs for the biomedical and cancer diagnosis applications.
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Affiliation(s)
- Murugan Ramadurai
- Department of Physical Chemistry, School of Chemical Sciences, University of Madras, Guindy Campus, Chennai, Tamilnadu 600 025, India
| | - Ganapathy Rajendran
- Department of Biotechnology, School of Life Sciences, University of Madras, Guindy Campus, Chennai, Tamilnadu 600 025, India
| | - Thangapandian Sathya Bama
- Department of Physical Chemistry, School of Chemical Sciences, University of Madras, Guindy Campus, Chennai, Tamilnadu 600 025, India
| | - Pandurangan Prabhu
- Department of Physical Chemistry, School of Chemical Sciences, University of Madras, Guindy Campus, Chennai, Tamilnadu 600 025, India.
| | - Krishnan Kathiravan
- Department of Biotechnology, School of Life Sciences, University of Madras, Guindy Campus, Chennai, Tamilnadu 600 025, India.
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25
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Qureshi A, Niazi JH. Biosensors for detecting viral and bacterial infections using host biomarkers: a review. Analyst 2020; 145:7825-7848. [DOI: 10.1039/d0an00896f] [Citation(s) in RCA: 15] [Impact Index Per Article: 3.0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 02/06/2023]
Abstract
A schematic diagram showing multiple modes of biosensing platforms for the diagnosis of bacterial or viral infections.
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Affiliation(s)
- Anjum Qureshi
- Sabanci University
- SUNUM Nanotechnology Research and Application Center
- Tuzla 34956
- Turkey
| | - Javed H. Niazi
- Sabanci University
- SUNUM Nanotechnology Research and Application Center
- Tuzla 34956
- Turkey
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Rajendran K, Rajendran G, Kasthuri J, Kathiravan K, Rajendiran N. Sweet Corn
(Zea mays L. var. rugosa)
Derived Fluorescent Carbon Quantum Dots for Selective Detection of Hydrogen Sulfide and Bioimaging Applications. ChemistrySelect 2019. [DOI: 10.1002/slct.201903385] [Citation(s) in RCA: 6] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/29/2022]
Affiliation(s)
- Kalimuthu Rajendran
- Department of Polymer ScienceUniversity of MadrasGuindy Campus, Chennai-25, Tamil Nadu India
| | - Ganapathy Rajendran
- Department of BiotechnologyUniversity of MadrasGuindy Campus, Chennai-25, Tamil Nadu India
| | - Jayapalan Kasthuri
- Department of ChemistryQuaid-E- Millath Govt. College for Women, Chennai-2 Tamil Nadu
| | - Krishnan Kathiravan
- Department of BiotechnologyUniversity of MadrasGuindy Campus, Chennai-25, Tamil Nadu India
| | - Nagappan Rajendiran
- Department of Polymer ScienceUniversity of MadrasGuindy Campus, Chennai-25, Tamil Nadu India
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Alende-Castro V, Alonso-Sampedro M, Vazquez-Temprano N, Tuñez C, Rey D, García-Iglesias C, Sopeña B, Gude F, Gonzalez-Quintela A. Factors influencing erythrocyte sedimentation rate in adults: New evidence for an old test. Medicine (Baltimore) 2019; 98:e16816. [PMID: 31441853 PMCID: PMC6716712 DOI: 10.1097/md.0000000000016816] [Citation(s) in RCA: 55] [Impact Index Per Article: 9.2] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Indexed: 12/18/2022] Open
Abstract
The erythrocyte sedimentation rate (ESR) is a routine test for inflammation. Few studies have investigated the potential influence of lifestyle factors and common metabolic abnormalities on the ESR. This study investigates the influence of demographic factors, alcohol consumption, smoking, physical activity, obesity, and metabolic syndrome on the ESR in adults.This cross-sectional study covered 1472 individuals (44.5% males; age range, 18-91 years) randomly selected from the population of a Spanish municipality. The ESR was measured using a standardized method. We assessed habitual alcohol consumption in standard drinking units, along with tobacco smoking, regular physical exercise (by questionnaire), body mass index, and variables defining metabolic syndrome. Multivariate analyses were performed, including mean corpuscular volume and hemoglobin concentration in the models.The ESR was higher in females than in males, and increased steadily with age. Median ESR of females was 2-fold higher than that of males, and median ESR of individuals aged >65 years was 2-fold higher than that of individuals in the youngest category (ages 18-35 years). Body mass index, presence of metabolic syndrome, and smoking were independently and positively associated with higher ESR values. Light alcohol drinkers and individuals with high regular physical activity displayed lower ESR values than did alcohol abstainers and individuals with low physical activity, respectively.ESR varies greatly with age and sex, and corresponding reference values are proposed. Lifestyle factors (physical activity, smoking, and alcohol consumption) and common metabolic abnormalities (obesity and related metabolic syndrome) may also influence ESR values.
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Affiliation(s)
- Vanessa Alende-Castro
- Department of Internal Medicine, Complejo Hospitalario Universitario, University of Santiago de Compostela
| | - Manuela Alonso-Sampedro
- Department of Internal Medicine, Complejo Hospitalario Universitario, University of Santiago de Compostela
- Department of Clinical Epidemiology, Complejo Hospitalario Universitario, Santiago de Compostela
| | - Nuria Vazquez-Temprano
- Department of Internal Medicine, Complejo Hospitalario Universitario, University of Santiago de Compostela
| | | | | | | | - Bernardo Sopeña
- Department of Internal Medicine, Complejo Hospitalario Universitario, University of Santiago de Compostela
| | - Francisco Gude
- Department of Clinical Epidemiology, Complejo Hospitalario Universitario, Santiago de Compostela
| | - Arturo Gonzalez-Quintela
- Department of Internal Medicine, Complejo Hospitalario Universitario, University of Santiago de Compostela
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Fernandes FS, da Silva GS, Hilel AS, Carvalho AC, Remor KVT, Schlindwein AD, Kanis LA, Martins DF, Kviecinski MR. Study of the potential adverse effects caused by the dermal application of Dillenia indica L. fruit extract standardized to betulinic acid in rodents. PLoS One 2019; 14:e0217718. [PMID: 31150479 PMCID: PMC6544281 DOI: 10.1371/journal.pone.0217718] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.2] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 02/18/2019] [Accepted: 05/16/2019] [Indexed: 12/17/2022] Open
Abstract
This study aimed to evaluate the potential adverse effects of the dermal administration of Dillenia indica Linnaeus (D. indica) fruit extract in healthy rodents; the extract was standardized to betulinic acid. In the initial phase, the acute effects were evaluated on the skin application site of a single extract dose. A skin irritation test was performed in male Wistar rats (n = 8/group) receiving the extract (50–150 mg/mL) with betulinic acid (0.5–1.5%, respectively). A photosensitivity test was performed in male BALB/c mice (n = 6/group) receiving the extract (150 mg/mL). Afterwards, other BALB/c mice (n = 20, male:female, 1:1) were used to assess the systemic alterations caused by 14 daily repeated doses (150 mg/mL) by monitoring the effects on mortality, body morphology, behavior, nutrition status, neuromotor reactions, organ morphology and weight, and blood tests. At this time, 0.5 mg/mL clobetasol was used as the positive control. The skin irritation index suggested that negligible skin irritation had occurred, even when the extract was applied to the rat skin at 150 mg/mL. However, the extract acted as a photosensitizer on mouse skin, showing a photosensitizing activity close to that of 10 mg/mL 5-methoxypsoralen. Repeated doses caused no mouse mortality, aggressiveness, piloerection, diarrhea, convulsions, neuromotor alterations or nutrition status changes. The mouse organ weights did not change, and the mice did not have alterations in their blood compositions. Clobetasol caused a reduction in the mononuclear leukocyte numbers. In general, the data suggest that the extract was safe in healthy rodents but indicate that caution should be taken with the photosensitizing activity; in addition, this activity should be further explored as it may be useful for phototherapeutic drug development.
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Affiliation(s)
- Flávia S. Fernandes
- Universidade do Sul de Santa Catarina, Postgraduate Program in Health Sciences, Palhoça, Santa Catarina, Brazil
| | - Gustavo S. da Silva
- Universidade do Sul de Santa Catarina, Postgraduate Program in Health Sciences, Palhoça, Santa Catarina, Brazil
| | - Alexandre S. Hilel
- Universidade do Sul de Santa Catarina, Postgraduate Program in Health Sciences, Palhoça, Santa Catarina, Brazil
| | - Ana C. Carvalho
- Universidade do Sul de Santa Catarina, Postgraduate Program in Health Sciences, Palhoça, Santa Catarina, Brazil
| | - Karina V. T. Remor
- Universidade do Sul de Santa Catarina, Postgraduate Program in Health Sciences, Palhoça, Santa Catarina, Brazil
| | - Aline D. Schlindwein
- Universidade do Sul de Santa Catarina, Postgraduate Program in Health Sciences, Palhoça, Santa Catarina, Brazil
| | - Luiz A. Kanis
- Universidade do Sul de Santa Catarina, Postgraduate Program in Health Sciences, Palhoça, Santa Catarina, Brazil
| | - Daniel F. Martins
- Universidade do Sul de Santa Catarina, Postgraduate Program in Health Sciences, Palhoça, Santa Catarina, Brazil
| | - Maicon R. Kviecinski
- Universidade do Sul de Santa Catarina, Postgraduate Program in Health Sciences, Palhoça, Santa Catarina, Brazil
- * E-mail: ,
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Korkmaz Ö, Göksel S, Gül M, Başçil H, Yildir Y, Berkan Ö. Does the use of N-butyl-2 cyanoacrylate in the treatment of lower extremity superficial varicose veins cause acute systemic inflammation and allergic reactions? Cardiovasc J Afr 2019; 29:213-217. [PMID: 30204219 PMCID: PMC6421552 DOI: 10.5830/cvja-2018-012] [Citation(s) in RCA: 3] [Impact Index Per Article: 0.5] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Download PDF] [Journal Information] [Subscribe] [Scholar Register] [Received: 11/08/2016] [Accepted: 02/14/2018] [Indexed: 11/06/2022] Open
Abstract
INTRODUCTION In this study we used N-butyl-2 cyanoacrylate (NBCA), including dimethyl sulfoxide (DMSO), via the endovenous route, for mechanochemical ablation in the treatment of superficial venous insufficiency, in an attempt to establish whether an early systemic inflammatory response and an allergic reaction occurred in the patients. METHODS A total of 102 patients were treated with endovenous medical ablation in two centres between October 2015 and February 2016. This study was a two-centre, retrospective, non-randomised investigational study. Ablation treatment with endovenous NBCA was used in patients with C3 to C4b grade superficial venous insufficiency, according to the CEAP (clinical, aetiology, anatomy and pathophysiology) clinical classification, with sapheno-femoral junctional insufficiency and a reflux of 0.5 seconds and longer on duplex ultra-sonography. Pre-operative whole blood count, erythrocyte sedimentation rate (ESR), C-reactive protein (CRP) level and blood chemistry were studied in all patients on admission to the clinic, and repeated in the second hour post-intervention. RESULTS All patients were treated successfully. Pre-operative white blood cell count (WBC) was 6.82 ± 1.67 × 109 cells/µl, and post intervention it was 6.57 ± 1.49 × 109 cells/µl; the difference was not statistically significant (p = 0.68). The neutrophil count before the intervention was 4.09 ± 1.33 × 109 cells/µl, while afterwards, it was 4.09 ± 1.33 × 109 cells/µl, with no statistically significant difference (p = 0.833). Pre-intervention eosinophil count was 0.64 ± 1.51 × 109 cells/µl, while it was 0.76 ± 1.65 × 109 cells/µl after the intervention, and the difference was statistically significant. Pre-intervention ESR and CRP values were 18.92 ± 9.77 mm/h and 1.71 ± 1.54 mg/dl, respectively. Postoperative ESR and CRP values were 19.78 ± 15.90 mm/h and 1.73 ± 1.59 mg/dl, respectively, but the differences were not statistically significant. When the parameters were analysed by gender, the differences between pre- and postoperative WBC and eosinophil count, ESR and CRP in women were not statistically significant. On the other hand, although the change in WBC count and CRP value were not statistically significant in males, the differences in eosinophil count and ESR were statistically significant. CONCLUSIONS Cyanoacrylate has been used in the endovenous medical ablation of varicose veins and superficial venous insufficiency over the last few years without the use of thermal energy and tumescent anaesthesia, which represents the greatest advantage of this method. In addition, since it causes no systemic allergic or acute inflammatory reaction, it appears to be safe to use.
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Affiliation(s)
- Özge Korkmaz
- Department of Cardiovascular Surgery, Cumhuriyet University School of Medicine, Sivas, Turkey.
| | - Sabahattin Göksel
- Department of Cardiovascular Surgery, Cumhuriyet University School of Medicine, Sivas, Turkey
| | - Müslim Gül
- Sivas Numune State Hospital, Sivas, Turkey
| | | | - Yavuz Yildir
- Department of Medical Biology, Cumhuriyet University, Sivas, Turkey
| | - Öcal Berkan
- Department of Cardiovascular Surgery, Cumhuriyet University School of Medicine, Sivas, Turkey
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Fest J, Ruiter R, Mooijaart SP, Ikram MA, van Eijck CHJ, Stricker BH. Erythrocyte sedimentation rate as an independent prognostic marker for mortality: a prospective population-based cohort study. J Intern Med 2019; 285:341-348. [PMID: 30537394 DOI: 10.1111/joim.12853] [Citation(s) in RCA: 3] [Impact Index Per Article: 0.5] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Indexed: 11/28/2022]
Abstract
BACKGROUND A very high erythrocyte sedimentation rate (ESR) is usually an indication of underlying pathology. Additionally, a moderately elevated ESR may also be attributable to biological ageing. Whether the ESR is a prognostic factor for mortality, regardless of age, has been scarcely investigated. Therefore, the objective was to analyse the association between elevated ESR levels and the risk of mortality in a prospective cohort of the general population. METHODS We studied data from the Rotterdam Study (1990-2014). ESR levels were measured at baseline and individuals were followed until death or end of study. Associations between moderately (20-50 mm h-1 ) and markedly (>50 mm h-1 ) elevated ESR levels and all-cause mortality were assessed using multivariate Cox proportional hazard models. RESULTS In total, 5226 participants were included, and the mean age was 70.3 years. During a median follow-up time of 14.9 years, 3749 participants died (71.7%). After adjustment, both a moderately elevated ESR and a markedly elevated ESR were associated with a significantly higher risk of overall mortality [hazard ratio (HR) 1.23, 95% confidence interval (CI) 1.12-1.35 and HR 1.89, 95% CI 1.38-2.60, respectively]. Although the ESR becomes higher with age, in a group aged above 75 years, without any comorbidities, an ESR > 20 mm h-1 remained associated with a significantly increased risk of mortality (HR 1.29, 95%CI 1.01-1.64). CONCLUSION An elevated ESR is an independent prognostic factor for mortality. Despite the fact that ESR increases with age, it remains associated with an increased risk of mortality and warrants close follow-up.
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Affiliation(s)
- J Fest
- Department of Surgery, Erasmus MC University Medical Center, Rotterdam, The Netherlands.,Department of Epidemiology, Erasmus MC University Medical Center, Rotterdam, The Netherlands
| | - R Ruiter
- Department of Epidemiology, Erasmus MC University Medical Center, Rotterdam, The Netherlands.,Department of Internal Medicine, Leiden University Medical Center, Leiden, The Netherlands
| | - S P Mooijaart
- Department of Internal Medicine, Leiden University Medical Center, Leiden, The Netherlands.,Institute for Evidence-based Medicine in Old Age, Leiden, The Netherlands
| | - M A Ikram
- Department of Epidemiology, Erasmus MC University Medical Center, Rotterdam, The Netherlands
| | - C H J van Eijck
- Department of Surgery, Erasmus MC University Medical Center, Rotterdam, The Netherlands
| | - B H Stricker
- Department of Epidemiology, Erasmus MC University Medical Center, Rotterdam, The Netherlands
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Tuberculosis-associated anemia is linked to a distinct inflammatory profile that persists after initiation of antitubercular therapy. Sci Rep 2019; 9:1381. [PMID: 30718725 PMCID: PMC6361962 DOI: 10.1038/s41598-018-37860-5] [Citation(s) in RCA: 47] [Impact Index Per Article: 7.8] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 07/26/2018] [Accepted: 12/14/2018] [Indexed: 11/24/2022] Open
Abstract
Pulmonary tuberculosis (PTB) is associated with chronic inflammation and anemia. How anemia impacts systemic inflammation in PTB patients undergoing antitubercular therapy (ATT) is not fully understood. In the present study, data on several blood biochemical parameters were retrospectively analyzed from 118 PTB patients during the first 60 days of ATT. Multidimensional statistical analyses were employed to perform detailed inflammatory profiling of patients stratified by anemia status prior to treatment. Anemia was defined as hemoglobin levels <12.5 g/dL for female and <13.5 g/dL for male individuals. The findings revealed that most of anemia cases were likely caused by chronic inflammation. A distinct biosignature related to anemia was detected, defined by increased values of uric acid, C-reactive protein, and erythrocyte sedimentation rate. Importantly, anemic patients sustained increased levels of several biochemical markers at day 60 of therapy. Preliminary analysis failed to demonstrate association between persistent inflammation during ATT with frequency of positive sputum cultures at day 60. Thus, TB patients with anemia exhibit a distinct inflammatory profile, which is only partially reverted at day 60 of ATT.
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Juskewitch JE, Norgan AP, Johnson RD, Trivedi VA, Hanson CA, Block DR. Impact of an electronic decision support rule on ESR/CRP co-ordering rates in a community health system and projected impact in the tertiary care setting and a commercially insured population. Clin Biochem 2019; 66:13-20. [PMID: 30711389 DOI: 10.1016/j.clinbiochem.2019.01.009] [Citation(s) in RCA: 2] [Impact Index Per Article: 0.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 10/23/2018] [Revised: 01/18/2019] [Accepted: 01/29/2019] [Indexed: 11/25/2022]
Abstract
INTRODUCTION Erythrocyte sedimentation rate (ESR) and C-reactive protein (CRP) are common laboratory assays used as markers of inflammation. ESR suffers from higher false positive and false negative rates than CRP. To that end, the American Board of Internal Medicine's (ABIM's) Choosing Wisely campaign has recommended against ESR testing for those with undiagnosed conditions in favor of CRP testing. This study describes the impact of a computerized provider order entry (CPOE) decision support rule against ESR/CRP co-ordering within a community health system that predates the ABIM's Choosing Wisely national guidance. To demonstrate the potential impact of such a CPOE rule within other healthcare settings, ESR/CRP ordering data from a multi-site tertiary care practice and from the commercially insured population in the OptumLabs® Data Warehouse (OLDW) were analyzed and the relative reduction in ESR/CRP co-ordering achieved within the community health system was projected onto these populations. MATERIALS AND METHODS ESR and/or CRP orders from a community health system were assessed from 2012 to 2016. Co-ordering and test concordance rates between ESR and CRP were compared before and after CPOE decision support rule launch. Similarly, ESR/CRP co-ordering across three tertiary care sites from 2015 to 2016 and the OLDW from 2009 to 2013 were assessed and the co-ordering rate reduction achieved in the community health system was mathematically projected onto these populations. Estimated payer savings from the rule's effect were calculated within each population using Medicare reimbursement rates. RESULTS The CPOE decision support rule realized an unadjusted 42% relative rate reduction in ESR/CRP co-ordering within the community health system yielding an annual payer savings of $15,000 with a modest increase in ESR/CRP concordance rates. Projecting a 40% relative reduction in ESR/CRP co-ordering rates from a similarly effective CPOE rule, annual payer cost reductions exceeding $100,000 within a multi-site tertiary care setting and $1,000,000 within the OLDW would be expected. CONCLUSION ESR/CRP co-ordering represents an opportunity to eliminate testing waste and reduce payer costs. A CPOE decision support rule stably reduces ESR/CRP co-ordering rates. Similar results may occur as one component of new commercially available decision support platforms.
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Affiliation(s)
- Justin E Juskewitch
- Department of Laboratory Medicine & Pathology, Mayo Clinic, Rochester 55905, MN, USA
| | - Andrew P Norgan
- Department of Laboratory Medicine & Pathology, Mayo Clinic, Rochester 55905, MN, USA
| | - Ryan D Johnson
- Robert D. and Patricia E. Kern Center for the Science of Health Care Delivery, Mayo Clinic, Rochester 55905, MN, USA; OptumLabs, Cambridge 02142, MA, USA; Population Health Innovation Institute, Department of Care Delivery, MetroHealth System, Cleveland 44109, OH, USA
| | - Vipul A Trivedi
- Department of Laboratory Medicine & Pathology, Mayo Clinic Health System, Eau Claire 54703, WI, USA
| | - Curtis A Hanson
- Department of Laboratory Medicine & Pathology, Mayo Clinic, Rochester 55905, MN, USA
| | - Darci R Block
- Department of Laboratory Medicine & Pathology, Mayo Clinic, Rochester 55905, MN, USA.
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Kornum JB, Farkas DK, Sværke C, Severinsen MT, Thomsen RW, Sørensen HT. Cancer Risk and Prognosis after a Hospital Contact for an Elevated Erythrocyte Sedimentation Rate. Cancer Epidemiol Biomarkers Prev 2018; 28:225-232. [PMID: 30352816 DOI: 10.1158/1055-9965.epi-18-0376] [Citation(s) in RCA: 5] [Impact Index Per Article: 0.7] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 04/05/2018] [Revised: 06/15/2018] [Accepted: 10/16/2018] [Indexed: 11/16/2022] Open
Abstract
BACKGROUND An elevated erythrocyte sedimentation rate (ESR) may be a marker of occult cancer. METHODS We linked Danish medical databases to examine cancer incidence in patients with a first-time hospital contact for elevated ESR during 1980 to 2013. We calculated standardized incidence ratios (SIR) of cancer compared with the general population, and comorbidity-adjusted HRs (aHR) versus matched population comparisons without elevated ESR. We also compared survival among patients with cancer with elevated ESR with that among patients with cancer without elevated ESR. RESULTS During median follow-up of 4.9 years, we observed 3,926 cancers among 18,540 patients with a first-time hospital contact for elevated ESR. The risk for any cancer diagnosed during the first year following the contact for elevated ESR was 8.5% [95% confidence interval (CI), 8.1%-8.9%]. The overall 1-year cancer incidence was markedly elevated [SIR 5.3 (95% CI, 5.1-5.6); aHR 5.8 (95% CI, 5.4-6.3)] and was more than 3-fold elevated for most hematologic cancers and for cancers of the peritoneum and connective tissue in the abdominal wall, kidney, and adrenal glands. After the first year, patients were at increased risk of developing especially hematologic cancers. Patients diagnosed with cancer within 1 year after a contact for elevated ESR had poorer survival compared with matched cancer comparisons [adjusted mortality rate ratio 1.2 (95% CI, 1.1-1.3)]. CONCLUSIONS Elevated ESR is a strong marker of undiagnosed cancer and is associated with poorer survival. IMPACT Our findings may help clinicians in assessing absolute risk, common sites, and prognosis of cancers discovered after hospital contact with elevated ERS.
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Affiliation(s)
- Jette B Kornum
- Department of Clinical Epidemiology, Aarhus University Hospital, Aarhus, Denmark. .,Department of Clinical Microbiology, Aalborg University Hospital, Aalborg, Denmark
| | - Dóra K Farkas
- Department of Clinical Epidemiology, Aarhus University Hospital, Aarhus, Denmark
| | - Claus Sværke
- Department of Clinical Epidemiology, Aarhus University Hospital, Aarhus, Denmark
| | | | - Reimar W Thomsen
- Department of Clinical Epidemiology, Aarhus University Hospital, Aarhus, Denmark
| | - Henrik T Sørensen
- Department of Clinical Epidemiology, Aarhus University Hospital, Aarhus, Denmark
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Jung SM, Kwok SK, Ju JH, Lee SW, Song JJ, Yoon CH, Park YB, Park SH. Risk factors associated with inadequate control of disease activity in elderly patients with rheumatoid arthritis: Results from a nationwide KOrean College of Rheumatology BIOlogics (KOBIO) registry. PLoS One 2018; 13:e0205651. [PMID: 30325962 PMCID: PMC6191131 DOI: 10.1371/journal.pone.0205651] [Citation(s) in RCA: 9] [Impact Index Per Article: 1.3] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/29/2017] [Accepted: 09/29/2018] [Indexed: 02/04/2023] Open
Abstract
OBJECTIVE The proportion of elderly patients with rheumatoid arthritis (RA) is continuously growing as a result of the increasing aging population. We compared disease activity between different age groups, and evaluated the clinical factors associated with high disease activity. METHODS This cross-sectional study analyzed the data of RA patients enrolled in the Korean College of Rheumatology Biologics registry (KOBIO-RA) between 2012 and 2014. Disease activity between elderly (age ≥ 65 years) and non-elderly patients (age < 65 years) was compared, and the association of clinical factors with high disease activity was assessed using a multivariate logistic regression model. RESULTS Of 1,227 patients in KOBIO-RA, 244 patients with RA were aged 65 years or over. In elderly patients, the proportion of men was higher (P = 0.012), and the duration of disease was longer (P < 0.001) compared with non-elderly patients. The elderly group showed a higher incidence of comorbidity (P < 0.001), and less use of methotrexate (P = 0.004). Assessment of disease activity using various composite measures showed a higher proportion of high disease activity in elderly patients than non-elderly patients. Longer disease duration, presence of comorbidity, and non-use of methotrexate were independently associated with high disease activity (P = 0.002, P < 0.001, and P = 0.029, respectively). CONCLUSIONS At enrollment of KOBIO-RA, elderly patients showed higher disease activity compared with non-elderly patients. Disease duration, use of methotrexate, and comorbidity are associated with disease activity control in Korean patients with RA.
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Affiliation(s)
- Seung Min Jung
- Division of Rheumatology, Department of Internal Medicine, Yonsei University College of Medicine, Seoul, Korea
| | - Seung-Ki Kwok
- Division of Rheumatology, Department of Internal Medicine, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea
| | - Ji Hyeon Ju
- Division of Rheumatology, Department of Internal Medicine, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea
| | - Sang-Won Lee
- Division of Rheumatology, Department of Internal Medicine, Yonsei University College of Medicine, Seoul, Korea
| | - Jason Jungsik Song
- Division of Rheumatology, Department of Internal Medicine, Yonsei University College of Medicine, Seoul, Korea
| | - Chong-Hyeon Yoon
- Division of Rheumatology, Department of Internal Medicine, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea
| | - Yong-Beom Park
- Division of Rheumatology, Department of Internal Medicine, Yonsei University College of Medicine, Seoul, Korea
| | - Sung-Hwan Park
- Division of Rheumatology, Department of Internal Medicine, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea
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Muthukumarasamyvel T, Rajendran G, Santhana Panneer D, Kasthuri J, Kathiravan K, Rajendiran N. Role of Surface Hydrophobicity of Dicationic Amphiphile-Stabilized Gold Nanoparticles on A549 Lung Cancer Cells. ACS OMEGA 2017; 2:3527-3538. [PMID: 30023697 PMCID: PMC6044882 DOI: 10.1021/acsomega.7b00353] [Citation(s) in RCA: 20] [Impact Index Per Article: 2.5] [Reference Citation Analysis] [Abstract] [Track Full Text] [Subscribe] [Scholar Register] [Received: 03/24/2017] [Accepted: 06/12/2017] [Indexed: 05/11/2023]
Abstract
Herein, we report the surface functionality of dicationic cysteamine conjugated cholic acid (DCaC), dicationic cysteamine conjugated deoxycholic acid (DCaDC), and dicationic cysteamine conjugated lithocholic acid (DCaLC) templated gold nanoparticles (AuNPs) on mammalian cells. The haemocompatibility of the synthesized NPs was evaluated by in vitro hemolysis and erythrocyte sedimentation rate using human red blood cells (RBCs). In all of the systems, no toxicity was observed on human erythrocytes (RBCs) up to the concentration of 120 μg/mL. The anticancer activity of these dicationic amphiphile-stabilized AuNPs on A549 lung cancer cells was demonstrated by in vitro cell viability assay, intracellular reactive oxygen species estimation by DCFH-DA, apoptosis analysis using AO-EtBr fluorescence staining, DNA fragmentation analysis by agarose gel electrophoresis, and western blot analysis of caspase-3 expression. These results suggest that the cytotoxicity of AuNPs to A549 cells increase with the dose and hydrophobicity of amphiphiles and were found to be in the order: DCaLC-AuNPs > DCaDC-AuNPs > DCaC-AuNPs.
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Affiliation(s)
- Thangavel Muthukumarasamyvel
- Department
of Polymer Science and Department of Biotechnology, University of Madras, Guindy Campus, Chennai 600025, Tamil Nadu, India
| | - Ganapathy Rajendran
- Department
of Polymer Science and Department of Biotechnology, University of Madras, Guindy Campus, Chennai 600025, Tamil Nadu, India
| | - Devendrapandi Santhana Panneer
- Department
of Polymer Science and Department of Biotechnology, University of Madras, Guindy Campus, Chennai 600025, Tamil Nadu, India
| | - Jayapalan Kasthuri
- Department
of Chemistry, Quaid-E-Millath Government
College for Women (Autonomous), Chennai 600002, Tamil Nadu, India
| | - Krishnan Kathiravan
- Department
of Polymer Science and Department of Biotechnology, University of Madras, Guindy Campus, Chennai 600025, Tamil Nadu, India
| | - Nagappan Rajendiran
- Department
of Polymer Science and Department of Biotechnology, University of Madras, Guindy Campus, Chennai 600025, Tamil Nadu, India
- E-mail:
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Pesce M, Tatangelo R, La Fratta I, Rizzuto A, Campagna G, Turli C, Ferrone A, Franceschelli S, Speranza L, Verrocchio MC, De Lutiis MA, Felaco M, Grilli A. Memory Training Program Decreases the Circulating Level of Cortisol and Pro-inflammatory Cytokines in Healthy Older Adults. Front Mol Neurosci 2017; 10:233. [PMID: 28790890 PMCID: PMC5522887 DOI: 10.3389/fnmol.2017.00233] [Citation(s) in RCA: 6] [Impact Index Per Article: 0.8] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 02/07/2017] [Accepted: 07/06/2017] [Indexed: 12/31/2022] Open
Abstract
Aging cognitive decline has been associated to impairment of the Hypothalamus Pituitary Adrenals (HPA) axis activity and a higher level of the systemic inflammation. However, little is known about the molecules driving this process at peripheral level. In addition, the cognitive function is to some extent modifiable with Memory Training (MT) programs, even among older adults and beyond. The study aims to evaluate whether MT could contribute to ameliorate cognitive performance and modulate the HPA axis activity as well the low level inflammation in the aging phenotype. Whether the phosphatase WIP-1, a negative regulator for inflammation, is involved in this process was also investigated. We recruited 31 young adults (19-28, years of age) and 62 older adults aged over 60. Thirty-two older adults were submitted to 6-months of MT program (EG), and 28 older adults were no treated and used as Control Group (CG). Global cognitive functioning (MMSE score), verbal and visual memory, and attention were assessed at baseline (T0) and after 6-months (T1). At the same time, plasmatic level of Cortisol (C), IL-1β, IL-18, IL-6, and the expression of WIP-1 mRNA and protein in ex vivo Peripheral Blood Mononuclear Cells were analyzed in young adults at T0, as well in older adults at T0 and T1. Together, the results suggest that MT improves the global cognitive functionality, verbal and visual memory, as well as the level of attention. At the same time we observed a decrease of the plasmatic level of C, of the cytokines, and an increase of the expression of mRNA and protein of WIP-1. The analysis of correlations highlighted that the level of the mRNA of WIP-1 was positively associated to the MMSE score, and negatively to the C and cytokine levels. In conclusion, we purpose the MT as tool that could help support successful aging through the improving of memory, attention and global cognitive function performance. Furthermore, this approach could participate to maintain lower the peripheral levels of the C and pro-inflammatory cytokines. The WIP-1 as a potential new target of the pathophysiology of aging is theorized.
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Affiliation(s)
| | - Raffaella Tatangelo
- School of Medicine and Health Science, University G. D’AnnunzioChieti, Italy
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Nishisako H, Kunishima H, Shimizu G, Teruya Y, Yokokawa M, Hirose M, Sakai T, Yamasaki Y, Tsuchida T, Naitou Y, Takashi O, Torikai K, Nakagawa T, Matsuda T. Investigation on prediction formulae for calculating erythrocyte sedimentation rate. J Gen Fam Med 2017; 18:146-147. [PMID: 29264012 PMCID: PMC5689405 DOI: 10.1002/jgf2.1] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.1] [Reference Citation Analysis] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 11/05/2014] [Accepted: 10/19/2015] [Indexed: 11/16/2022] Open
Affiliation(s)
- Hisashi Nishisako
- Division of General Internal Medicine; Department of Internal Medicine; St. Marianna University School of Medicine; Kanagawa Japan
- Division of General Internal Medicine; Department of Internal Medicine; Kawasaki Tama Municipal Hospital; Kanagawa Japan
| | - Hiroyuki Kunishima
- Division of General Internal Medicine; Department of Internal Medicine; St. Marianna University School of Medicine; Kanagawa Japan
- Division of General Internal Medicine; Department of Internal Medicine; Kawasaki Tama Municipal Hospital; Kanagawa Japan
| | - Gohji Shimizu
- Division of General Internal Medicine; Department of Internal Medicine; St. Marianna University School of Medicine; Kanagawa Japan
| | - Yoko Teruya
- Division of General Internal Medicine; Department of Internal Medicine; St. Marianna University School of Medicine; Kanagawa Japan
- Division of General Internal Medicine; Department of Internal Medicine; Kawasaki Tama Municipal Hospital; Kanagawa Japan
| | - Masatoshi Yokokawa
- Division of General Internal Medicine; Department of Internal Medicine; St. Marianna University School of Medicine; Kanagawa Japan
- Division of General Internal Medicine; Department of Internal Medicine; Kawasaki Tama Municipal Hospital; Kanagawa Japan
| | - Masanori Hirose
- Division of General Internal Medicine; Department of Internal Medicine; St. Marianna University School of Medicine; Kanagawa Japan
- Division of General Internal Medicine; Department of Internal Medicine; Kawasaki Tama Municipal Hospital; Kanagawa Japan
| | - Tsubasa Sakai
- Division of General Internal Medicine; Department of Internal Medicine; St. Marianna University School of Medicine; Kanagawa Japan
- Division of General Internal Medicine; Department of Internal Medicine; Kawasaki Tama Municipal Hospital; Kanagawa Japan
| | - Yukitaka Yamasaki
- Division of General Internal Medicine; Department of Internal Medicine; St. Marianna University School of Medicine; Kanagawa Japan
- Division of General Internal Medicine; Department of Internal Medicine; Kawasaki Tama Municipal Hospital; Kanagawa Japan
| | - Tomoya Tsuchida
- Division of General Internal Medicine; Department of Internal Medicine; St. Marianna University School of Medicine; Kanagawa Japan
- Division of General Internal Medicine; Department of Internal Medicine; Kawasaki Tama Municipal Hospital; Kanagawa Japan
| | - Yoshiyuki Naitou
- Division of General Internal Medicine; Department of Internal Medicine; St. Marianna University School of Medicine; Kanagawa Japan
| | - Ogihara Takashi
- Division of General Internal Medicine; Department of Internal Medicine; St. Marianna University School of Medicine; Kanagawa Japan
| | - Keito Torikai
- Division of General Internal Medicine; Department of Internal Medicine; St. Marianna University School of Medicine; Kanagawa Japan
| | - Teisuke Nakagawa
- Division of General Internal Medicine; Department of Internal Medicine; St. Marianna University School of Medicine; Kanagawa Japan
| | - Takahide Matsuda
- Division of General Internal Medicine; Department of Internal Medicine; St. Marianna University School of Medicine; Kanagawa Japan
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Hsiao CC, Weng CH, Li YJ, Wu HH, Chen YC, Chen YM, Hsu HH, Tian YC. Comparison of the clinical features and outcomes of infective endocarditis between hemodialysis and non-hemodialysis patients. Ther Clin Risk Manag 2017; 13:663-668. [PMID: 28579790 PMCID: PMC5449118 DOI: 10.2147/tcrm.s135262] [Citation(s) in RCA: 6] [Impact Index Per Article: 0.8] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/23/2022] Open
Abstract
Hemodialysis (HD) patients are more susceptible to infective endocarditis (IE) due to the increased risk of bacterial invasion through intravascular access. However, it remains unclear whether the causative organisms and outcomes of IE in HD patients differ from those in non-HD patients. This study clarified the differences in clinical presentation and outcomes between HD and non-HD patients. At our hospital, we performed a retrospective study of 39 HD and 51 non-HD patients with echocardiography-confirmed IE between June 2000 and February 2007. No differences in sex, intravenous drug use, previous diagnosis of congestive heart failure, and previous valvular surgery were observed between these two groups. The number of patients with diabetic mellitus in these two groups was significantly different (28.2% HD vs 5.9% non-HD patients). The C-reactive protein levels in the two groups were not significantly different. By contrast, the erythrocyte sedimentation rate was significantly higher in the HD patients (HD vs non-HD: 87.2±33.32 vs 52.96±28.19). The incidence of IE involving the mitral valve (MV; 45.1%) or the aortic valve (AV; 43.1%) was similar among the non-HD patients, whereas a preference of IE involving the MV (79.5%) over the AV (15.4%) was noted among the HD patients. The HD patients had a significantly higher Staphylococcus aureus infection rate (HD: 46.2%; non-HD: 27.5%). The proportion of methicillin-resistant S. aureus (MRSA; 83.8%) infection accounting for S. aureus IE in the HD group was higher than that (28.6%) in the non-HD group. The in-hospital mortality rate did not differ between the two groups. In conclusion, compared with non-HD patients, a propensity of IE involving the MV and a higher MRSA infection rate were observed in HD patients. The in-hospital mortality rate of echocardiography-confirmed IE did not differ between the two groups.
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Affiliation(s)
- Ching-Chung Hsiao
- Kidney Research Center and Department of Nephrology, Chang Gung Memorial Hospital, Taipei
| | - Cheng-Hao Weng
- Kidney Research Center and Department of Nephrology, Chang Gung Memorial Hospital, Taipei.,Graduate Institute of Clinical Medical Sciences, Chang Gung University, Taoyuan, Taiwan
| | - Yi-Jung Li
- Kidney Research Center and Department of Nephrology, Chang Gung Memorial Hospital, Taipei
| | - Hsin-Hsu Wu
- Kidney Research Center and Department of Nephrology, Chang Gung Memorial Hospital, Taipei.,Graduate Institute of Clinical Medical Sciences, Chang Gung University, Taoyuan, Taiwan
| | - Yung-Chang Chen
- Kidney Research Center and Department of Nephrology, Chang Gung Memorial Hospital, Taipei
| | - Yu-Ming Chen
- Kidney Research Center and Department of Nephrology, Chang Gung Memorial Hospital, Taipei
| | - Hsiang-Hao Hsu
- Kidney Research Center and Department of Nephrology, Chang Gung Memorial Hospital, Taipei
| | - Ya-Chung Tian
- Kidney Research Center and Department of Nephrology, Chang Gung Memorial Hospital, Taipei
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Benvenuti M, An T, Amaro E, Lovejoy S, Mencio G, Martus J, Mignemi M, Schoenecker JG. Double-Edged Sword: Musculoskeletal Infection Provoked Acute Phase Response in Children. Orthop Clin North Am 2017; 48:181-197. [PMID: 28336041 DOI: 10.1016/j.ocl.2016.12.009] [Citation(s) in RCA: 20] [Impact Index Per Article: 2.5] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 02/02/2023]
Abstract
The acute phase response has a crucial role in mounting the body's response to tissue injury. Excessive activation of the acute phase response is responsible for many complications that occur in orthopedic patients. Given that infection may be considered continuous tissue injury that persistently activates the acute phase response, children with musculoskeletal infections are at markedly increased risk for serious complications. Future strategies that modulate the acute phase response have the potential to improve treatment and prevent complications associated with musculoskeletal infection.
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Affiliation(s)
| | - Thomas An
- Vanderbilt University School of Medicine, Nashville, TN, USA
| | - Emilie Amaro
- Vanderbilt University School of Medicine, Nashville, TN, USA
| | - Steven Lovejoy
- Department of Orthopaedics, Vanderbilt University Medical Center, Nashville, TN, USA
| | - Gregory Mencio
- Department of Orthopaedics, Vanderbilt University Medical Center, Nashville, TN, USA
| | - Jeffrey Martus
- Department of Orthopaedics, Vanderbilt University Medical Center, Nashville, TN, USA
| | - Megan Mignemi
- Department of Orthopaedics, Vanderbilt University Medical Center, Nashville, TN, USA
| | - Jonathan G Schoenecker
- Department of Orthopaedics, Vanderbilt University Medical Center, Nashville, TN, USA; Departments of Orthopaedics, Pharmacology, and Pathology, Microbiology and Immunology, Vanderbilt University Medical Center, Nashville, Tennessee.
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Birtane M, Yavuz S, Taştekin N. Laboratory evaluation in rheumatic diseases. World J Methodol 2017; 7:1-8. [PMID: 28396844 PMCID: PMC5366934 DOI: 10.5662/wjm.v7.i1.1] [Citation(s) in RCA: 11] [Impact Index Per Article: 1.4] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 08/26/2016] [Revised: 12/21/2016] [Accepted: 01/18/2017] [Indexed: 02/06/2023] Open
Abstract
Autoantibodies can help clinicians to allow early detection of autoimmune diseases and their clinical manifestations, to determine effective monitoring of prognosis and the treatment response. From this point, they have a high impact in rheumatic disease management. When used carefully they allow rapid diagnosis and appropriate treatment. However, as they may be present in healthy population they may cause confusion for interpreting the situation. False positive test results may lead to wrong treatment and unnecessary anxiety for patients. Autoantibody positivity alone does not make a diagnosis. Similarly, the absence of autoantibodies alone does not exclude diagnosis. The success of the test is closely related to sensitivity, specificity and likelihood ratios. So, interpretation of these is very important for a proper laboratory evaluation. In conclusion, in spite of the remarkable advances in science and technology, a deeply investigated anamnesis and comprehensive physical examination still continue to be the best diagnostic method. The most correct approach is that clinicians apply laboratory tests to confirm or exclude preliminary diagnosis based on anamnesis and physical examination. This review will discuss these issues.
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Bahmani L, Neysari M, Maleki M. The study of drying and pattern formation of whole human blood drops and the effect of thalassaemia and neonatal jaundice on the patterns. Colloids Surf A Physicochem Eng Asp 2017. [DOI: 10.1016/j.colsurfa.2016.10.065] [Citation(s) in RCA: 36] [Impact Index Per Article: 4.5] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/30/2022]
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Osagie I, Ofunre E, Kenneth A. Assessment of some biomarkers of inflammation in breast cancer patients in Benin City, Nigeria. ACTA ACUST UNITED AC 2017. [DOI: 10.4103/njss.njss_2_14] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.1] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/04/2022]
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Isiksacan Z, Erel O, Elbuken C. A portable microfluidic system for rapid measurement of the erythrocyte sedimentation rate. LAB ON A CHIP 2016; 16:4682-4690. [PMID: 27858026 DOI: 10.1039/c6lc01036a] [Citation(s) in RCA: 31] [Impact Index Per Article: 3.4] [Reference Citation Analysis] [Abstract] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 05/25/2023]
Abstract
The erythrocyte sedimentation rate (ESR) is a frequently used 30 min or 60 min clinical test for screening of several inflammatory conditions, infections, trauma, and malignant diseases, as well as non-inflammatory conditions including prostate cancer and stroke. Erythrocyte aggregation (EA) is a physiological process where erythrocytes form face-to-face linear structures, called rouleaux, at stasis or low shear rates. In this work, we proposed a method for ESR measurement from EA. We developed a microfluidic opto-electro-mechanical system, using which we experimentally showed a significant correlation (R2 = 0.86) between ESR and EA. The microfluidic system was shown to measure ESR from EA using fingerprick blood in 2 min. 40 μl of whole blood is filled in a disposable polycarbonate cartridge which is illuminated with a near infrared emitting diode. Erythrocytes were disaggregated under the effect of a mechanical shear force using a solenoid pinch valve. Following complete disaggregation, transmitted light through the cartridge was measured using a photodetector for 1.5 min. The intensity level is at its lowest at complete disaggregation and highest at complete aggregation. We calculated ESR from the transmitted signal profile. We also developed another microfluidic cartridge specifically for monitoring the EA process in real-time during ESR measurement. The presented system is suitable for ultrafast, low-cost, and low-sample volume measurement of ESR at the point-of-care.
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Affiliation(s)
- Ziya Isiksacan
- Institute of Materials Science and Nanotechnology, National Nanotechnology Research Center (UNAM), Bilkent University, Ankara, 06800, Turkey.
| | - Ozcan Erel
- Yıldırım Beyazit University Faculty of Medicine, Ankara, Turkey
| | - Caglar Elbuken
- Institute of Materials Science and Nanotechnology, National Nanotechnology Research Center (UNAM), Bilkent University, Ankara, 06800, Turkey.
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Abstract
Pediatricians and other pediatric health care providers in primary care settings are often faced with the challenge of evaluating patients with suspected autoimmune disorders. The first and most important step in evaluating children with suspected autoimmune disorders is taking a detailed history and performing a thorough physical examination. This step helps narrow the diagnosis, and thus helps determine appropriate laboratory evaluations. The results of laboratory tests need to be interpreted within the clinical context. It is also important to recognize that the laboratory tests have different diagnostic values in children as compared to adults. [Pediatr Ann. 2016;45(7):e265-e271.].
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Inflammatory glycoproteins in cardiometabolic disorders, autoimmune diseases and cancer. Clin Chim Acta 2016; 459:177-186. [PMID: 27312321 DOI: 10.1016/j.cca.2016.06.012] [Citation(s) in RCA: 54] [Impact Index Per Article: 6.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 04/07/2016] [Revised: 06/08/2016] [Accepted: 06/11/2016] [Indexed: 12/25/2022]
Abstract
The physiological function initially attributed to the oligosaccharide moieties or glycans on inflammatory glycoproteins was to improve protein stability. However, it is now clear that glycans play a prominent role in glycoprotein structure and function and in some cases contribute to disease states. In fact, glycan processing contributes to pathogenicity not only in autoimmune disorders but also in atherosclerotic cardiovascular disease, diabetes and malignancy. While most clinical laboratory tests measure circulating levels of inflammatory proteins, newly developed diagnostic and prognostic tests are harvesting the information that can be gleaned by measuring the amount or structure of the attached glycans, which may be unique to individuals as well as various diseases. As such, these newer glycan-based tests may provide future means for more personalized approaches to patient stratification and improved patient care. Here we will discuss recent progress in high-throughput laboratory methods for glycomics (i.e. the study of glycan structures) and glycoprotein quantification by methods such as mass spectrometry and nuclear magnetic resonance spectroscopy. We will also review the clinical utility of glycoprotein and glycan measurements in the prediction of common low-grade inflammatory disorders including cardiovascular disease, diabetes and cancer, as well as for monitoring autoimmune disease activity.
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Dima A, Opris D, Jurcut C, Baicus C. Is there still a place for erythrocyte sedimentation rate and C-reactive protein in systemic lupus erythematosus? Lupus 2016; 25:1173-9. [PMID: 27256317 DOI: 10.1177/0961203316651742] [Citation(s) in RCA: 44] [Impact Index Per Article: 4.9] [Reference Citation Analysis] [Abstract] [Key Words] [Journal Information] [Subscribe] [Scholar Register] [Received: 12/20/2015] [Accepted: 04/20/2016] [Indexed: 11/15/2022]
Abstract
The inflammatory response during systemic lupus erythematosus (SLE) flares is known to be atypical, characterized by a disproportionately lower C-reactive protein (CRP) elevation when compared with erythrocyte sedimentation rate (ESR). Thus, in these patients, the analysis of inflammatory markers might be challenging in daily clinical practice. Clinicians need frequently to distinguish lupus reactivations and infectious conditions, and the significance of ESR and CRP seems to be different. Even though a non-specific marker of inflammation, ESR utility in SLE should not be neglected and it appears to be a useful biomarker for SLE activity assessment. Describing a specific cut-off for ESR in SLE is important for patients' follow-up, and levels up to 25-30 mm/h have been proposed as an upper limit of the normal range. Regarding CRP, even though higher baseline levels are described in SLE when compared with controls, including in remission periods, its response during flares seems to be incomplete and not always correlated with disease activity; while CRP values greater than 10 mg/l could be indicative for severe flares, when there is no serositis or arthritis, higher CRP levels above 50-60 mg/l may be associated with infection.
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Affiliation(s)
- A Dima
- Colentina Clinical Hospital, Colentina Research Center, Bucharest, Romania Carol Davila University of Medicine and Pharmacy, Bucharest, Romania
| | - D Opris
- Carol Davila University of Medicine and Pharmacy, Bucharest, Romania Sfanta Maria Clinical Hospital, Bucharest, Romania
| | - C Jurcut
- Carol Davila Central University Emergency Military Hospital, Bucharest, Romania
| | - C Baicus
- Colentina Clinical Hospital, Colentina Research Center, Bucharest, Romania Carol Davila University of Medicine and Pharmacy, Bucharest, Romania
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Al Musawi MS, Jaafar MS, Al-Gailani B, Ahmed NM, Suhaimi FM, Bakhsh M. Erythrocyte sedimentation rate of human blood exposed to low-level laser. Lasers Med Sci 2016; 31:1195-201. [PMID: 27250712 DOI: 10.1007/s10103-016-1972-1] [Citation(s) in RCA: 13] [Impact Index Per Article: 1.4] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 01/27/2016] [Accepted: 05/24/2016] [Indexed: 11/24/2022]
Abstract
This study is designed to investigate in vitro low-level laser (LLL) effects on rheological parameter, erythrocyte sedimentation rate (ESR), of human blood. The interaction mechanism between LLL radiation and blood is unclear. Therefore, research addresses the effects of LLL irradiation on human blood and this is essential to understanding how laser radiation interacts with biological cells and tissues. The blood samples were collected through venipuncture into EDTA-containing tubes as an anticoagulant. Each sample was divided into two equal aliquots to be used as a non-irradiated sample (control) and an irradiated sample. The aliquot was subjected to doses of 36, 54, 72 and 90 J/cm(2) with wavelengths of 405, 589 and 780 nm, with a radiation source at a fixed power density of 30 mW/cm(2). The ESR and red blood cell count and volume are measured after laser irradiation and compared with the non-irradiated samples. The maximum reduction in ESR is observed with radiation dose 72 J/cm(2) delivered with a 405-nm wavelength laser beam. Moreover, no hemolysis is observed under these irradiation conditions. In a separate protocol, ESR of separated RBCs re-suspended in irradiated plasma (7.6 ± 2.3 mm/h) is found to be significantly lower (by 51 %) than their counterpart re-suspended in non-irradiated plasma (15.0 ± 3.7 mm/h). These results indicate that ESR reduction is mainly due to the effects of LLL on the plasma composition that ultimately affect whole blood ESR.
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Affiliation(s)
- Mustafa S Al Musawi
- School of Physics, Universiti Sains Malaysia, 11800, Pulau Pinang, Malaysia. .,Department of Physiology, College of Medicine, Al-Mustansiriya University, Baghdad, Iraq.
| | - M S Jaafar
- School of Physics, Universiti Sains Malaysia, 11800, Pulau Pinang, Malaysia
| | - B Al-Gailani
- Department of Physiology, College of Medicine, Al-Mustansiriya University, Baghdad, Iraq
| | - Naser M Ahmed
- School of Physics, Universiti Sains Malaysia, 11800, Pulau Pinang, Malaysia
| | - Fatanah M Suhaimi
- Advanced Medical and Dental Institute, Universiti Sains Malaysia, Bertam, 13200, Kepala Batas P. Pinang, Malaysia
| | - Muhammad Bakhsh
- School of Physics, Universiti Sains Malaysia, 11800, Pulau Pinang, Malaysia
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