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Yan X, Li Q, Huang X, Li K, Wang Q, Liu H. Adsorption of gaseous arsenic enhanced by lattice oxygen activation in pyrolusite: Method and mechanism. ENVIRONMENTAL RESEARCH 2025; 271:121125. [PMID: 39954925 DOI: 10.1016/j.envres.2025.121125] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 12/03/2024] [Revised: 02/08/2025] [Accepted: 02/12/2025] [Indexed: 02/17/2025]
Abstract
Atmospheric arsenic pollution from industrial flue gas emissions poses severe environmental and health risks, and necessitates the development of more efficient removal strategies. This study presents a novel approach for enhancing the As2O3(g) adsorption capacity of pyrolusite (PY) by activating its lattice oxygen to generate oxygen vacancies through ball milling. The activated PY can achieve an adsorption capacity of 13.84 mg/g at 300 °C, which is 1.6 times higher than that of pristine PY. Both experimental results and density functional theory calculations reveal the significance of oxygen vacancies, demonstrating increased electronic PY activity and improved lattice oxygen mobility. Furthermore, the lattice oxygen-mediated oxidation-adsorption pathway is elucidated, highlighting the pivotal role of oxygen vacancies in enhancing adsorption performance. This study provides an innovative pathway for designing highly efficient adsorbents for As2O3(g) removal, offering new insights into lattice oxygen activation mechanisms.
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Affiliation(s)
- Xuelei Yan
- School of Metallurgy and Environment, Central South University, Changsha 410083, China; National Engineering Research Centre for Control and Treatment of Heavy Metal Pollution, Central South University, Changsha 410083, China
| | - Qingzhu Li
- School of Metallurgy and Environment, Central South University, Changsha 410083, China; National Engineering Research Centre for Control and Treatment of Heavy Metal Pollution, Central South University, Changsha 410083, China.
| | - Xiaowei Huang
- National Engineering Research Center for Rare Earth Materials, General Research Institute for Non-Ferrous Metals, Beijing 100088, China
| | - Kaizhong Li
- School of Metallurgy and Environment, Central South University, Changsha 410083, China; National Engineering Research Centre for Control and Treatment of Heavy Metal Pollution, Central South University, Changsha 410083, China
| | - Qingwei Wang
- School of Metallurgy and Environment, Central South University, Changsha 410083, China; National Engineering Research Centre for Control and Treatment of Heavy Metal Pollution, Central South University, Changsha 410083, China.
| | - Hui Liu
- School of Metallurgy and Environment, Central South University, Changsha 410083, China; National Engineering Research Centre for Control and Treatment of Heavy Metal Pollution, Central South University, Changsha 410083, China
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Bhattacharya RS, Singh R, Panghal A, Thakur A, Singh L, Verma RK, Singh C, Goyal M, Kumar J. Multi-Targeting Phytochemicals for Alzheimer's Disease. Phytother Res 2025; 39:1453-1483. [PMID: 39815655 DOI: 10.1002/ptr.8435] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 05/01/2024] [Revised: 11/23/2024] [Accepted: 12/28/2024] [Indexed: 01/18/2025]
Abstract
Alzheimer's disease (AD) is a type of neurodegenerative illness in which β-amyloid (Aβ) and tau protein accumulate in neurons in the form of tangles. The pathophysiological pathway of AD consists of Aβ-amyloid peptides, tau proteins, and oxidative stress in neurons and increased neuro-inflammatory response. Food and Drug Administration in the United States has authorized various drugs for the effective treatment of AD, which include galantamine, rivastigmine, donepezil, memantine, sodium oligomannate, lecanemab, and aducanumab. The major disadvantage of these drugs is that they only provide "symptomatic" relief. They are most effective in the early stages or for mild to moderate cases of the disease, but are not suitable for long-term use. Besides conventional therapies, phytochemicals have the potential to stop the progression of AD. According to research, the use of potential phytochemicals against AD has gained attention due to their potent anti-inflammatory, antioxidant, anti-hyperphosphorylation of the tau protein, metal chelation, and anti-amyloid properties. This study seeks to provide an up-to-date compilation of the most current and promising breakthroughs in AD therapy using phytochemicals. It could be concluded that phytochemicals light serve as an effective therapy for AD. However, more mechanistic investigations are needed to determine the clinical implications of phytochemicals in AD treatment.
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Affiliation(s)
- Radha Shree Bhattacharya
- Department of Pharmaceutical Sciences, Hemwati Nandan Bahuguna Garhwal University (A Central University), Srinagar, Uttarakhand, India
| | - Raghuraj Singh
- Institute of Nano Science and Technology (INST), Mohali, Punjab, India
- Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, Uttar Pradesh, India
| | - Archna Panghal
- Department of Pharmacology and Toxicology, Facility for Risk Assessment and Intervention Studies, National Institute of Pharmaceutical Education and Research (NIPER), S.A.S Nagar, Punjab, India
| | - Ashima Thakur
- Faculty of Pharmaceutical Sciences, Himachal Pradesh, India
| | - Lachhman Singh
- Faculty of Pharmacy, Government Pharmacy College, Seraj, V.P.O. Bagsaid, Mandi, Himachal Pradesh, India
| | - Rahul Kumar Verma
- Institute of Nano Science and Technology (INST), Mohali, Punjab, India
- Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, Uttar Pradesh, India
| | - Charan Singh
- Department of Pharmaceutical Sciences, Hemwati Nandan Bahuguna Garhwal University (A Central University), Srinagar, Uttarakhand, India
| | - Manoj Goyal
- Department of Pharmaceutical Sciences, Hemwati Nandan Bahuguna Garhwal University (A Central University), Srinagar, Uttarakhand, India
| | - Jayant Kumar
- Department of Pharmaceutical Sciences, Hemwati Nandan Bahuguna Garhwal University (A Central University), Srinagar, Uttarakhand, India
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Balali-Mood M, Eizadi-Mood N, Hassanian-Moghaddam H, Etemad L, Moshiri M, Vahabzadeh M, Sadeghi M. Recent advances in the clinical management of intoxication by five heavy metals: Mercury, lead, chromium, cadmium and arsenic. Heliyon 2025; 11:e42696. [PMID: 40040983 PMCID: PMC11876891 DOI: 10.1016/j.heliyon.2025.e42696] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/10/2024] [Revised: 12/15/2024] [Accepted: 02/12/2025] [Indexed: 03/06/2025] Open
Abstract
Metals have been used for many centuries, but their nutritional and toxic effects have been investigated since the last century. The common toxic heavy metals (THM) include mercury, lead, chromium cadmium, and arsenic. As human exposure to THM increasingly causes systemic and organ complications, it seems required to review the recent advances of treatment of the toxic metals. Despite the current knowledge of the hazards of heavy metals, there is still high incidents of their poisonings particularly in developing countries. In this review, after an introduction, we briefly describe the routes of exposure, clinical features and prognosis of each metal poisoning. Then, review the different treatments for each metal with particular attention to recent advances in the treatment of both acute and chronic poisonings. The main antidotes for all THM are still chelating agents, but new agents were developed over the past decades and have been used successfully for the THM poisonings. Dimercaptosuccinic acid (DMSA) known as succimer has been prescribed as a safe oral chelator in lead poisoning. Similarly, dimercapto-propanesulfonic acid (DMPS) has also revealed fewer side effects than the old chelating agents. The two are currently gaining increased acceptance among clinical toxicologists. However, there is no specific antidote for mercury poisoning. Dimercaprol is almost no longer used as an antidote of choice in the treatment of chronic THM poisoning. Comparison of clinical management of intoxication by the five heavy metals reveals similar treatment strategies. On the other hand, some of them require specific interventions to reduce the toxicity. Because of drawbacks in the application of commonly known chelating agents, treatment with bioactive compounds which have antioxidant and anti-inflammatory properties has been the subject of much interest in recent research. However, despite the promising results observed in experimental animals, clinical trials on their clinical therapeutic benefits have not been yet successful and need further studies to determine their efficacy and safety in humans. Development of less toxic chelating agents are still under investigations. Moreover, the development of orally administrable chelating agents for home health care would likely be of great interest for future research.
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Affiliation(s)
- Mahdi Balali-Mood
- Medical Toxicology and Drug Abuse Research Center, Birjand University of Medical Sciences, Birjand, Iran
| | - Nastaran Eizadi-Mood
- Department of Clinical Toxicology, School of Medicine, Isfahan Clinical Toxicology Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
| | - Hossein Hassanian-Moghaddam
- Social Determinants of Health Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
- Royal Perth Bentley Group, Next Step Drug and Alcohol Services, Perth, Australia
| | - Leila Etemad
- Medical Toxicology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
- Pharmaceutical Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran
- Department of Pharmaceutical and Food Control, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
| | - Mohammad Moshiri
- Medical Toxicology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
| | - Maryam Vahabzadeh
- Medical Toxicology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
| | - Mahmood Sadeghi
- Medical Toxicology and Drug Abuse Research Center, Birjand University of Medical Sciences, Birjand, Iran
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Adeogun AE, Ogunleye OD, Akhigbe TM, Oyedokun PA, Adegbola CA, Saka WA, Afolabi OA, Akhigbe RE. Impact of arsenic on male and female reproductive function: a review of the pathophysiology and potential therapeutic strategies. NAUNYN-SCHMIEDEBERG'S ARCHIVES OF PHARMACOLOGY 2025; 398:1283-1297. [PMID: 39287676 DOI: 10.1007/s00210-024-03452-6] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 08/03/2024] [Accepted: 09/10/2024] [Indexed: 09/19/2024]
Abstract
Arsenic is a ubiquitous metalloid and heavy metal that contributes to the global decline in human fertility. Humans are constantly exposed to arsenic through biotic and abiotic sources, especially ingestion of arsenic-contaminated food and water. Its exposure is associated with several adverse health challenges, including reproductive toxicity. In spite of its reported adverse effects, arsenic exposure remains a global challenge. Hence, this study provides a comprehensive review of the literature on the impact and mechanism of arsenic on male and female reproductive function. Additionally, a review of the potential therapeutic strategies is presented. Evidence from the literature reveals that arsenic upregulates reactive oxygen species (ROS) generation which mediates arsenic-induced suppression of the hypothalamic-pituitary-gonadal axis and inactivation of 3β-HSD and 17β-HSD activities, leading to reduced gonadal steroidogenesis. Through several oxidative stress-dependent signaling, arsenic induces the apoptosis of the germ cells, thus contributing to the development of infertility. At the moment, there is no specific treatment for arsenic-induced reproductive toxicity. However, increasing data form the scientific literature reveals the benefits of antioxidants in ameliorating arsenic-induced reproductive toxicity. These molecules suppress ROS generation and maintain optimal activities of the hypothalamic-pituitary-gonadal axis, leading to optimal steroidogenesis and gametogenesis as well as improved germ cells. Overall, this study revealed the impact and associated mechanism of arsenic-induced reproductive toxicity. It also provides evidence from the literature demonstrating potential therapeutic measures in managing arsenic-induced reproductive toxicity.
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Affiliation(s)
- A E Adeogun
- Department of Physiology, Babcock University, Ilishan Remo, Ogun State, Nigeria
- Reproductive Biology and Toxicology Research Laboratory, Oasis of Grace Hospital, Osogbo, Osun State, Nigeria
| | - O D Ogunleye
- Reproductive Biology and Toxicology Research Laboratory, Oasis of Grace Hospital, Osogbo, Osun State, Nigeria
- Department of Physiology, Ladoke Akintola University of Technology, Ogbomoso, Oyo State, Nigeria
| | - T M Akhigbe
- Reproductive Biology and Toxicology Research Laboratory, Oasis of Grace Hospital, Osogbo, Osun State, Nigeria
- Department of Agronomy, Osun State University, Ejigbo Campus, Osogbu, Osun State, Nigeria
| | - P A Oyedokun
- Reproductive Biology and Toxicology Research Laboratory, Oasis of Grace Hospital, Osogbo, Osun State, Nigeria
- Department of Physiology, Ladoke Akintola University of Technology, Ogbomoso, Oyo State, Nigeria
| | - C A Adegbola
- Reproductive Biology and Toxicology Research Laboratory, Oasis of Grace Hospital, Osogbo, Osun State, Nigeria
- Department of Physiology, Ladoke Akintola University of Technology, Ogbomoso, Oyo State, Nigeria
| | - W A Saka
- Department of Physiology, Ladoke Akintola University of Technology, Ogbomoso, Oyo State, Nigeria
| | - O A Afolabi
- Department of Physiology, Ladoke Akintola University of Technology, Ogbomoso, Oyo State, Nigeria
| | - R E Akhigbe
- Reproductive Biology and Toxicology Research Laboratory, Oasis of Grace Hospital, Osogbo, Osun State, Nigeria.
- Department of Physiology, Ladoke Akintola University of Technology, Ogbomoso, Oyo State, Nigeria.
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Waidande SS, Kshirsagar M, Thorat VM, Tiwari DD. Role of Antioxidant Supplementation in Enhancing Chelation Therapy for Lead-Induced Oxidative Stress in Rats. Cureus 2025; 17:e79699. [PMID: 40161109 PMCID: PMC11952131 DOI: 10.7759/cureus.79699] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 02/08/2025] [Accepted: 02/26/2025] [Indexed: 04/02/2025] Open
Abstract
Background and aim Lead poisoning is an increasingly critical issue in environmental and public health due to its ability to induce oxidative stress (OS), which contributes to various diseases. While chelation therapy is widely used to lower blood lead levels, it does not fully counteract the OS caused by lead exposure. Recent research suggests that combining chelation therapy with antioxidants may enhance its effectiveness by mitigating oxidative damage. This study evaluates whether supplementing chelation therapy with vitamin C can improve its efficacy in reducing OS in lead-exposed rats. Material and methods Forty male Wistar rats were divided into four groups: a control group, a lead-exposed group, a chelation therapy group, and a group that was given both chelation therapy and antioxidants. Serum malondialdehyde (MDA) levels and superoxide dismutase (SOD) activity, catalase activity, and glutathione (GSH) levels were determined to assess the efficiency of the treatment. Results The outcomes revealed that chelation therapy lowered some of the OS indicators, but this was much more pronounced when carrying out chelation therapy in combination with antioxidant intake. More particularly, the combination therapy group had an MDA level closer to control and significantly higher SOD and catalase activity, and nearly normal GSH levels. Conclusion The results of the present study indicate that antioxidant supplementation might provide synergistic support to chelation therapy for the management of lead-induced OS.
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Affiliation(s)
| | | | - Vandana M Thorat
- Pharmacology and Therapeutics, Krishna Institute of Medical Sciences, Karad, IND
| | - Devkumar D Tiwari
- Pharmacology, Krishna Vishwa Vidyapeeth (Deemed to be University), Karad, IND
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Konečný L, Lomozová Z, Karabanovich G, Roh J, Vávrová K, Mladěnka P. Rapid method for screening of both calcium and magnesium chelation with comparison of 21 known metal chelators. J Biol Inorg Chem 2024; 29:785-800. [PMID: 39422739 PMCID: PMC11638374 DOI: 10.1007/s00775-024-02078-6] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/15/2024] [Accepted: 10/03/2024] [Indexed: 10/19/2024]
Abstract
Chelation is the rational treatment modality in metal overload conditions, but chelators are often non-selective and can, hence, cause an imbalance in the homeostasis of physiological metals including calcium and magnesium. The aim of this study was to develop an affordable, rapid but sensitive and precise method for determining the degree of chelation of calcium and magnesium ions and to employ this method for comparison on a panel of known metal chelators. Spectrophotometric method using o-cresolphthalein complexone (o-CC) was developed and its biological relevance was confirmed in human platelets by impedance aggregometry. The lowest detectable concentration of calcium and magnesium ions by o-CC was 2.5 μM and 2 μM, respectively. The indicator was stable for at least 110 days. Four and seven out of twenty-one chelators strongly chelated calcium and magnesium ions, respectively. Importantly, the chelation effect of clinically used chelators was not negligible. Structure-activity relationships for eight quinolin-8-ols showed improvements in chelation particularly in the cases of dihalogen substitution, and a negative linear relationship between pKa and magnesium chelation was observed. Calcium chelation led to inhibition of platelet aggregation in concentrations corresponding to the complex formation. A novel method for screening of efficacy and safety of calcium and magnesium ion chelation was developed and validated.
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Affiliation(s)
- Lukáš Konečný
- Department of Pharmacology and Toxicology, Faculty of Pharmacy in Hradec Králové, Charles University, 50003, Hradec Králové, Czechia
| | - Zuzana Lomozová
- Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmacy in Hradec Králové, Charles University, 50003, Hradec Králové, Czechia
| | - Galina Karabanovich
- Department of Organic and Bioorganic Chemistry, Faculty of Pharmacy in Hradec Králové, Charles University, 50003, Hradec Králové, Czechia
| | - Jaroslav Roh
- Department of Organic and Bioorganic Chemistry, Faculty of Pharmacy in Hradec Králové, Charles University, 50003, Hradec Králové, Czechia
| | - Kateřina Vávrová
- Department of Organic and Bioorganic Chemistry, Faculty of Pharmacy in Hradec Králové, Charles University, 50003, Hradec Králové, Czechia
| | - Přemysl Mladěnka
- Department of Pharmacology and Toxicology, Faculty of Pharmacy in Hradec Králové, Charles University, 50003, Hradec Králové, Czechia.
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Yan X, Chen X, Zhang X, Qureshi A, Wang Y, Tang X, Hu T, Zhuang H, Ran X, Ma G, Luo P, Shen L. Proteomic analysis of the effects of Dictyophora polysaccharide on arsenic-induced hepatotoxicity in rats. Exp Mol Pathol 2024; 138:104910. [PMID: 38876078 DOI: 10.1016/j.yexmp.2024.104910] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/20/2023] [Revised: 05/21/2024] [Accepted: 06/03/2024] [Indexed: 06/16/2024]
Abstract
Arsenic (As) is a highly toxic environmental toxicant and a known human carcinogen. Long-term exposure to As can cause liver injury. Dictyophora polysaccharide (DIP) is a biologically active natural compound found in the Dictyophora with excellent antioxidation, anti-inflammation, and immune protection properties. In this study, the Sprague-Dawley (SD) rat model of As toxicity was established using a feeding method, followed by DIP treatment in rats with As-induced liver injury. The molecular mechanisms of As toxicity to the rat liver and the protective effect of DIP were investigated by proteomic studies. The results showed that 172, 328 and 191 differentially expressed proteins (DEPs) were identified between the As-exposed rats versus control rats (As/Ctrl), DIP treated rats versus As-exposed rats (DIP+As/As), and DIP treated rats versus control rats (DIP+As /Ctrl), respectively. Among them, the expression of 90 DEPs in the As/Ctrl groups was reversed by DIP treatment. As exposure caused dysregulation of metabolic pathways, mitochondria, oxidative stress, and apoptosis-related proteins in the rat liver. However, DIP treatment changed or restored the levels of these proteins, which attenuated the damage to the livers of rats caused by As exposure. The results provide new insights into the mechanisms of liver injury induced by As exposure and the treatment of DIP in As poisoning.
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Affiliation(s)
- Xi Yan
- School of Public Health, The Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Guizhou Medical University, Guiyang 561113, PR China
| | - Xiaolu Chen
- School of Public Health, The Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Guizhou Medical University, Guiyang 561113, PR China
| | - Xinglai Zhang
- School of Public Health, The Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Guizhou Medical University, Guiyang 561113, PR China
| | - Ayesha Qureshi
- College of Life Science and Oceanography, Shenzhen University, Shenzhen 518060, PR China
| | - Yi Wang
- School of Public Health, The Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Guizhou Medical University, Guiyang 561113, PR China
| | - Xiaoxiao Tang
- College of Life Science and Oceanography, Shenzhen University, Shenzhen 518060, PR China
| | - Ting Hu
- School of Public Health, The Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Guizhou Medical University, Guiyang 561113, PR China
| | - Hongbin Zhuang
- College of Life Science and Oceanography, Shenzhen University, Shenzhen 518060, PR China
| | - Xiaoqian Ran
- School of Public Health, The Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Guizhou Medical University, Guiyang 561113, PR China
| | - Guanwei Ma
- School of Public Health, The Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Guizhou Medical University, Guiyang 561113, PR China
| | - Peng Luo
- School of Public Health, The Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Guizhou Medical University, Guiyang 561113, PR China.
| | - Liming Shen
- School of Public Health, The Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Guizhou Medical University, Guiyang 561113, PR China; College of Life Science and Oceanography, Shenzhen University, Shenzhen 518060, PR China.
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Saleh SM, El-Tawil OS, Mahmoud MB, Abd El-Rahman SS, El-Saied EM, Noshy PA. Do Nanoparticles of Calcium Disodium EDTA Minimize the Toxic Effects of Cadmium in Female Rats? Biol Trace Elem Res 2024; 202:2228-2240. [PMID: 37721680 PMCID: PMC10955038 DOI: 10.1007/s12011-023-03842-6] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 05/26/2023] [Accepted: 09/01/2023] [Indexed: 09/19/2023]
Abstract
The present study aims to investigate the ability of CaNa2EDTA (ethylenediaminetetraacetic acid) macroparticles and nanoparticles to treat cadmium-induced toxicity in female rats and to compare their efficacies. Forty rats were divided into 4 equal groups: control, cadmium, cadmium + CaNa2EDTA macroparticles and Cd + CaNa2EDTA nanoparticles. Cadmium was added to the drinking water in a concentration of 30 ppm for 10 weeks. CaNa2EDTA macroparticles and nanoparticles (50 mg/kg) were intraperitoneally injected during the last 4 weeks of the exposure period. Every two weeks, blood and urine samples were collected for determination of urea, creatinine, metallothionein and cadmium concentrations. At the end of the experiment, the skeleton of rats was examined by X-ray and tissue samples from the kidney and femur bone were collected and subjected to histopathological examination. Exposure to cadmium increased the concentrations of urea and creatinine in the serum and the concentrations of metallothionein and cadmium in serum and urine of rats. A decrease in bone mineralization by X-ray examination in addition to various histopathological alterations in the kidney and femur bone of Cd-intoxicated rats were also observed. Treatment with both CaNa2EDTA macroparticles and nanoparticles ameliorated the toxic effects induced by cadmium on the kidney and bone. However, CaNa2EDTA nanoparticles showed a superior efficacy compared to the macroparticles and therefore can be used as an effective chelating antidote for treatment of cadmium toxicity.
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Affiliation(s)
- Safa M Saleh
- Department of Toxicology, Forensic Medicine and Veterinary Regulations, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt
| | - Osama S El-Tawil
- Department of Toxicology, Forensic Medicine and Veterinary Regulations, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt
| | - Manal B Mahmoud
- Immune Section, Research Institute for Animal Reproduction, Giza, Egypt
| | - Sahar S Abd El-Rahman
- Department of Pathology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt
| | - Eiman M El-Saied
- Department of Toxicology, Forensic Medicine and Veterinary Regulations, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt
| | - Peter A Noshy
- Department of Toxicology, Forensic Medicine and Veterinary Regulations, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.
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9
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Kumar K, Singh D. Toxicity and bioremediation of the lead: a critical review. INTERNATIONAL JOURNAL OF ENVIRONMENTAL HEALTH RESEARCH 2024; 34:1879-1909. [PMID: 36617394 DOI: 10.1080/09603123.2023.2165047] [Citation(s) in RCA: 1] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 10/07/2022] [Accepted: 12/27/2022] [Indexed: 06/17/2023]
Abstract
Lead is a naturally occurring, bluish-gray metal that is found in small quantities in the earth's crust. The existing literature demonstrates that non-biodegradable character and continuous use results in accumulation of lead concentration in the environment and causes various ill effects such as neurotoxicity, change in psychological and behavioral development of different organisms. Nowadays the most effective technique in the revival of the environment is bioremediation and it is environmentally friendly and cost-effective. Bacterial strains such as Oceanobacillus profundus and Lactobacillus acidophilus ATCC4356 have the ability to reduce lead 97% and 73.9%, respectively. Similarly some species of algae and fungal strains also showed lead removal efficiency as 74% (spirulina), 97.1% (Chlorella kessleri), 95.5% (Penicillium janthinillum) and 86% (Aspergillus flavus). Biodegradation of lead by various microbes would be the most efficient and sustainable approach. This review focuses on toxicity, fate of lead in the environment and its microbial degradation.
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Affiliation(s)
- Khushhal Kumar
- Department of Zoology, Central University of Jammu, Rahya-Suchani, Samba, Jammu and Kashmir, India
| | - Devinder Singh
- Department of Zoology, Chandigarh University, Mohali, Punjab, India
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10
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Wei X, Nicoletto C, Sambo P, Liu J, Wang J, Petrini R, Renella G. Thallium uptake and risk in vegetables grown in pyrite past-mining contaminated soil amended with organic fertilizer (compost): A potential method for Tl contamination remediation. THE SCIENCE OF THE TOTAL ENVIRONMENT 2024; 908:168002. [PMID: 37875191 DOI: 10.1016/j.scitotenv.2023.168002] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 06/07/2023] [Revised: 10/04/2023] [Accepted: 10/19/2023] [Indexed: 10/26/2023]
Abstract
Thallium (Tl) is a highly toxic trace metal that can cause severe pollution and damage to the ecological system. In this study, a field trial was conducted in a Tl-rich pyrite-barite past-mining area to unveil the fate of Tl in agricultural practice. Tuscany kale and red chicory cultivated in soil impacted by the dismissed mine of Valdicastello Carducci (Northern Tuscany, Italy) displayed significantly different uptake behaviors of Tl. Hyper-accumulation of Tl was observed in kale leaves and its content reached up to 17.1 mg kg-1 whereas only <0.70 mg kg-1 of Tl was found in leaves of red chicory. Due to the regionally polymetallic pollution, Tuscany kale grown in this area possessed a great Tl intake risk for the residents. As for the fertilization treatment, Tl in Tuscany kale leaves fertilized with mineral fertilizer (NPK) and compost were 21.4 and 12.8 mg kg-1. The results suggested a potential remediation ability of compost in diminishing Tl in the vegetable leaves and thus may reduce its risk in the soil-crop system. Since Tl poisoning emergency may occur in agricultural fields near past-mining zones, it is critical to establish possible remediation measures to ensure food safety surrounding former mining areas likewise.
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Affiliation(s)
- Xudong Wei
- Department of Agronomy, Food, Natural resources, Animals and Environment (DAFNAE), University of Padova, Viale dell'Università, 16, 35020 Legnaro, PD, Italy; School of Environmental Science and Engineering, Guangzhou University, Guangzhou 510006, China
| | - Carlo Nicoletto
- Department of Agronomy, Food, Natural resources, Animals and Environment (DAFNAE), University of Padova, Viale dell'Università, 16, 35020 Legnaro, PD, Italy.
| | - Paolo Sambo
- Department of Agronomy, Food, Natural resources, Animals and Environment (DAFNAE), University of Padova, Viale dell'Università, 16, 35020 Legnaro, PD, Italy
| | - Juan Liu
- School of Environmental Science and Engineering, Guangzhou University, Guangzhou 510006, China.
| | - Jin Wang
- School of Environmental Science and Engineering, Guangzhou University, Guangzhou 510006, China
| | - Riccardo Petrini
- Department of Earth Sciences, University of Pisa, Via S. Maria 53, 56126 Pisa, Italy
| | - Giancarlo Renella
- Department of Agronomy, Food, Natural resources, Animals and Environment (DAFNAE), University of Padova, Viale dell'Università, 16, 35020 Legnaro, PD, Italy
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11
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Idrees M, Ali S, Rehman A, Zajif Hussain S, Abbas Bukhari D. Uptake of lead by bacteria isolated from industrial effluents and their potential use in bioremediation of wastewater. Saudi J Biol Sci 2023; 30:103740. [PMID: 37538349 PMCID: PMC10393803 DOI: 10.1016/j.sjbs.2023.103740] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 05/31/2023] [Revised: 07/04/2023] [Accepted: 07/08/2023] [Indexed: 08/05/2023] Open
Abstract
Due to rising populations and human activities, heavy metals (HM) toxicity has become a serious problem for all life forms. The present study deals with isolating and identifying lead-resistant bacteria from contaminated wastewater of tanneries effluents. Two isolated strains were identified as Bacillus cereus (ID1), and Bacillus sp. (ID3), and both strains resisted a 25 mM concentration of Lead nitrate (Pb (NO3)2). After four days of treatment, Bacillus cereus (ID1) showed 80% lead uptake, and Bacillus sp. (ID3) showed 88%. Lead uptake was confirmed by Energy dispersive X-Ray (EDX) analysis. Fourier transform infrared spectroscopy (FTIR) showed that structural alterations had occurred in functional groups of the treated samples compared to the controls. Our research indicates that these Bacillus strains may be useful in bioremediating heavy metals from polluted environments. Further investigation into the processes involved in the uptake and homeostasis of heavy metals by these strains is required, as is the identification of the genes and enzymes responsible for Pb-bioremediation.
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Affiliation(s)
- Muhammad Idrees
- Department of Zoology, Government College University, Lahore, Pakistan
| | - Shakir Ali
- Department of Zoology, Government College University, Lahore, Pakistan
| | - Abdul Rehman
- Institute of Microbiology and Molecular Genetics, University of the Punjab, Lahore, Pakistan
| | - Syed Zajif Hussain
- Department of Chemistry, SBA School of Science and Engineering (SBASSE), Lahore University of Management Sciences (LUMS), DHA, Lahore Cantt 54792, Pakistan
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Alam T, Shahid F, Abidi S, Parwez I, Khan F. Thymoquinone supplementation mitigates arsenic-induced cytotoxic and genotoxic alterations in rat liver. J Trace Elem Med Biol 2022; 74:127067. [PMID: 36155422 DOI: 10.1016/j.jtemb.2022.127067] [Citation(s) in RCA: 2] [Impact Index Per Article: 0.7] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 03/20/2022] [Revised: 08/22/2022] [Accepted: 09/08/2022] [Indexed: 12/01/2022]
Abstract
Arsenic, a widespread environmental toxin, produces multiple organ toxicity, including hepatotoxicity. Thymoquinone (TQ) is known to restore liver functions in several hepatic injury models. This study aims to assess the mitigative potential of TQ against sodium arsenate (NaAs)-induced cytotoxic and genotoxic alterations in the liver. Rats were randomly distributed to control, NaAs, TQ, and NaAs+TQ groups. NaAs+TQ and TQ group of rats were pre-treated with TQ (1.5 mg/kg bwt, orally) for 14 days, and the treatment was further continued for 30 days, with and without NaAs treatment (5 mg/kg bwt, orally), respectively. The deleterious histological alterations in the liver of arsenic intoxicated animals were accompanied by an upsurge in the activities of serum ALT and AST, the diagnostic indicators of liver injury. NaAs caused pronounced alterations in the activities of membrane marker and carbohydrate metabolic enzymes and the enzymatic and non-enzymatic components of hepatic antioxidant defense. Significant hepatocyte DNA damage and hepatic arsenic accumulation were also observed in arsenic-exposed rats. TQ supplementation alleviated these adverse alterations and improved the overall hepatic metabolic and antioxidant status in NaAs-administered rats. Prevention of oxidative injury could be the key mechanism of TQ-elicited protective effects. TQ may have an excellent scope as a dietary supplement in the management of arsenic-induced hepatic pathophysiology.
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Affiliation(s)
- Tauseef Alam
- Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, Aligarh 202002, Uttar Pradesh, India
| | - Faaiza Shahid
- Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, Aligarh 202002, Uttar Pradesh, India
| | - Subuhi Abidi
- Department of Zoology, Faculty of Life Sciences, Aligarh Muslim University, Aligarh 202002, Uttar Pradesh, India
| | - Iqbal Parwez
- Department of Zoology, Faculty of Life Sciences, Aligarh Muslim University, Aligarh 202002, Uttar Pradesh, India
| | - Farah Khan
- Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, Aligarh 202002, Uttar Pradesh, India.
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Linšak Ž, Gobin I, Linšak DT, Broznić D. Effects of Long-Term Lead Exposure on Antioxidant Enzyme Defense System in Organs of Brown Hare (Lepus europaeus Pallas) as a Bioindicator of Environmental Pollution in Croatia. Biol Trace Elem Res 2022; 200:5091-5103. [PMID: 34981425 DOI: 10.1007/s12011-021-03090-6] [Citation(s) in RCA: 2] [Impact Index Per Article: 0.7] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 11/01/2021] [Accepted: 12/27/2021] [Indexed: 11/25/2022]
Abstract
In Croatia, Podravina is a well-known lead-polluted region due to the intensive exploitation of natural gas, a highly developed agricultural industry, and a traffic hub with several heavily traveled roads. It represents a natural environment with a great variety of wildlife, especially hares (Lepus europaeus Pallas), which may serve as an indicator for environmental quality assessment. This study was conducted to estimate the bioaccumulation of lead in hare liver, kidney, muscle, and brain during long-term exposure and its impact on the oxidative status of the organism and to investigate a possible lead exchange ionic mechanism in the brain. In the organs of two hare groups (experimental from polluted area and control from the island of Krk), Ca, Fe, Mg, Na, lead concentrations, and antioxidant enzyme defense system were analyzed. The accumulation of lead was highest in the brain (3.7-fold higher compared to the control group) and lowest in the liver (1.6-fold higher compared to the control group). Kendall-Tau and multiple regression analysis showed that the increased lead content caused a stronger exchange of Ca and Na ions in the brain. We proposed that lead either mobilizes intracellular cation stores or causes competitive displacement of Ca from the binding site in intoxicated cells. A linear predictive model for cell intoxication by lead was proposed, where GPx and SOD were predominantly influenced by long-term lead exposure. The presented results showed that long-term lead exposure in hares negatively affected their oxidative status and caused the strongest toxicity in the brain and muscles, making their survival and/or population vulnerable.
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Affiliation(s)
- Željko Linšak
- Department for Health Ecology, Faculty of Medicine, University of Rijeka, Rijeka, Croatia
- Teaching Institute of Public Health of Primorje-Gorski Kotar County, Rijeka, Croatia
| | - Ivana Gobin
- Department for Microbiology, Faculty of Medicine, University of Rijeka, Rijeka, Croatia
| | - Dijana Tomić Linšak
- Department for Health Ecology, Faculty of Medicine, University of Rijeka, Rijeka, Croatia.
- Teaching Institute of Public Health of Primorje-Gorski Kotar County, Rijeka, Croatia.
| | - Dalibor Broznić
- Department for Medical Chemistry, Biochemistry and Clinical Chemistry, Faculty of Medicine, University of Rijeka, Rijeka, Croatia
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Mutagenic, Carcinogenic, and Teratogenic Effect of Heavy Metals. EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE 2022; 2022:8011953. [PMID: 36248437 PMCID: PMC9556253 DOI: 10.1155/2022/8011953] [Citation(s) in RCA: 10] [Impact Index Per Article: 3.3] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 05/13/2022] [Revised: 08/10/2022] [Accepted: 09/16/2022] [Indexed: 11/23/2022]
Abstract
Heavy metal (HM)-induced toxicity and its associated complications have become a major issue in the medical world. HMs are not biodegradable, enter into the food chain, and gets accumulated in the living systems. Increased concentrations and accumulation of HMs can cause severely damaging effects and severe complications in living organisms and can even lead to the death of the organism. In Ayurvedic medicine, ingredients of natural origin, including whole plants or certain portions of the plant, animal sources, and minerals, are used for therapeutic purposes as medicine, both alone and in combination. HM such as cadmium, copper, zinc, lead, chromium, nickel, and arsenic cause hazardous effects on animals, human health, and the environment. This review focuses on mutagenic, carcinogenic, and teratogenic effects of HM , mechanism, organ toxicity, available remedies in the market, and their side effects. Also, emphasis is given to alternative systems of medicine to treat HM toxicity.
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Flora SJS, Jain K, Panghal A, Patwa J. Chemistry, Pharmacology, and Toxicology of Monoisoamyl Dimercaptosuccinic Acid: A Chelating Agent for Chronic Metal Poisoning. Chem Res Toxicol 2022; 35:1701-1719. [PMID: 35972774 DOI: 10.1021/acs.chemrestox.2c00129] [Citation(s) in RCA: 7] [Impact Index Per Article: 2.3] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/16/2022]
Abstract
Arsenic, a metalloid, is known to cause deleterious effects in various body organs, particularly the liver, urinary bladder, and brain, and these effects are primarily mediated through oxidative stress. Chelation therapy has been considered one of the promising medical treatments for arsenic poisoning. Meso 2,3- dimercaptosuccinic acid (DMSA) has been recognized as one of the most effective chelating drugs to treat arsenic poisoning. However, the drug is compromised with a number of shortcomings, including the inability to treat chronic arsenic poisoning due to its extracellular distribution. Monoisoamyl 2,3-dimercaptosuccinic acid, one of the analogues of meso 2,3-dimeraptosuccinic acid (DMSA), is a lipophilic chelator and has shown promise to be considered as a potential future chelating agent/antidote not only for arsenic but also for a few other heavy metals like lead, mercury, cadmium, and gallium arsenide. The results from numerous studies carried out in the recent past, mainly from our group, strongly support the clinical application of MiADMSA. This review paper summarizes most of the scientific details including the chemistry, pharmacology, and safety profile of MiADMSA. The efficacy of MiADMSA mainly against arsenic toxicity but also a few other heavy metals was also discussed. We also reviewed a few other strategies in order to achieve the optimum effects of MiADMSA, like combination therapy using two chelating agents or coadministration of a natural and synthetic antioxidant (including phytomedicine) along with MiADMSA for treatment of metal/metalloid poisoning. We also briefly discussed the use of nanotechnology (nano form of MiADMSA i.e. nano-MiADMSA) and compared it with bulk MiADMSA. All these strategies have been shown to be beneficial in getting more pronounced therapeutic efficacy of MiADMSA, as an adjuvant or as a complementary agent, by significantly increasing the chelating efficacy of MiADMSA.
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Affiliation(s)
- Swaran J S Flora
- National Institute of Pharmaceutical Education and Research (NIPER), Raebareli, Bijnor-Sisendi Road, Near CRPF Base, Lucknow, Uttar Pradesh 226002, India.,National Institute of Pharmaceutical Education and Research (NIPER), Sector 67, Sahibzada Ajit Singh Nagar, Mohali, Punjab 160062, India
| | - Keerti Jain
- National Institute of Pharmaceutical Education and Research (NIPER), Raebareli, Bijnor-Sisendi Road, Near CRPF Base, Lucknow, Uttar Pradesh 226002, India
| | - Archna Panghal
- National Institute of Pharmaceutical Education and Research (NIPER), Sector 67, Sahibzada Ajit Singh Nagar, Mohali, Punjab 160062, India
| | - Jayant Patwa
- National Institute of Pharmaceutical Education and Research (NIPER), Raebareli, Bijnor-Sisendi Road, Near CRPF Base, Lucknow, Uttar Pradesh 226002, India
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16
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Rashno M, Sarkaki A, Ghaderi S, Khoshnam SE. Sesamin: Insights into its protective effects against lead-induced learning and memory deficits in rats. J Trace Elem Med Biol 2022; 72:126993. [PMID: 35550983 DOI: 10.1016/j.jtemb.2022.126993] [Citation(s) in RCA: 6] [Impact Index Per Article: 2.0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 01/22/2022] [Revised: 04/03/2022] [Accepted: 05/03/2022] [Indexed: 11/20/2022]
Abstract
BACKGROUND Lead (Pb) is one of the most hazardous pollutants that induce a wide spectrum of neurological changes such as learning and memory deficits. Sesamin, a phytonutrient of the lignan class, exhibits anti-inflammatory, anti-apoptotic, and neuroprotective properties. The present study was designed to investigate the effects of sesamin against Pb-induced learning and memory deficits, disruption of hippocampal theta and gamma rhythms, inflammatory response, inhibition of blood δ-aminolevulinic acid dehydratase (δ-ALA-D) activity, Pb accumulation, and neuronal loss in rats. METHODS Sesamin treatment (30 mg/kg/day; P.O.) was started simultaneously with Pb acetate exposure (500 ppm in standard drinking water) in rats, and they continued for eight consecutive weeks. RESULTS The results showed that chronic exposure to Pb disrupted the learning and memory functions in both passive-avoidance and water-maze tests, which was accompanied by increase in spectral theta power and theta/gamma ratio, and a decrease in spectral gamma power in the hippocampus. Additionally, Pb exposure resulted in an enhanced tumor necrosis factor-alpha (TNF-α) content, decreased interleukin-10 (IL-10) production, inhibited blood δ-ALA-D activity, increased Pb accumulation, and neuronal loss of rats. In contrast, sesamin treatment improved all the above-mentioned Pb-induced pathological changes. CONCLUSION This data suggests that sesamin could improve Pb-induced learning and memory deficits, possibly through amelioration of hippocampal theta and gamma rhythms, modulation of inflammatory status, restoration of the blood δ-ALA-D activity, reduction of Pb accumulation in the blood and the brain tissues, and prevention of neuronal loss.
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Affiliation(s)
| | - Alireza Sarkaki
- Persian Gulf Physiology Research Center, Medical Basic Sciences Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
| | - Shahab Ghaderi
- Department of Neuroscience, School of Science and Advanced Technologies in Medicine, Hamadan University of Medical Sciences, Hamadan, Iran; Neurophysiology Research Center, Hamadan University of Medical Sciences, Hamadan, Iran.
| | - Seyed Esmaeil Khoshnam
- Persian Gulf Physiology Research Center, Medical Basic Sciences Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
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Resveratrol and neuroprotection: an insight into prospective therapeutic approaches against Alzheimer's disease from bench to bedside. Mol Neurobiol 2022; 59:4384-4404. [PMID: 35545730 DOI: 10.1007/s12035-022-02859-7] [Citation(s) in RCA: 39] [Impact Index Per Article: 13.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 02/11/2022] [Accepted: 04/28/2022] [Indexed: 02/07/2023]
Abstract
Alzheimer's disease (AD) is the most common cause of dementia and cognitive impairment; yet, there is currently no treatment. A buildup of Aβ, tau protein phosphorylation, oxidative stress, and inflammation in AD is pathogenic. The accumulation of amyloid-beta (Aβ) peptides in these neurocognitive areas is a significant characteristic of the disease. Therefore, inhibiting Aβ peptide aggregation has been proposed as the critical therapeutic approach for AD treatment. Resveratrol has been demonstrated in multiple studies to have a neuroprotective, anti-inflammatory, and antioxidant characteristic and the ability to minimize Aβ peptides aggregation and toxicity in the hippocampus of Alzheimer's patients, stimulating neurogenesis and inhibiting hippocampal degeneration. Furthermore, resveratrol's antioxidant effect promotes neuronal development by activating the silent information regulator-1 (SIRT1), which can protect against the detrimental effects of oxidative stress. Resveratrol-induced SIRT1 activation is becoming more crucial in developing novel therapeutic options for AD and other diseases that have neurodegenerative characteristics. This review highlighted a better knowledge of resveratrol's mechanism of action and its promising therapeutic efficacy in treating AD. We also highlighted the therapeutic potential of resveratrol as an AD therapeutic agent, which is effective against neurodegenerative disorders.
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Abejón R. A Bibliometric Analysis of Research on Selenium in Drinking Water during the 1990-2021 Period: Treatment Options for Selenium Removal. INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH 2022; 19:5834. [PMID: 35627373 PMCID: PMC9140891 DOI: 10.3390/ijerph19105834] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.3] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 04/08/2022] [Revised: 05/01/2022] [Accepted: 05/04/2022] [Indexed: 02/01/2023]
Abstract
A bibliometric analysis based on the Scopus database was carried out to summarize the global research related to selenium in drinking water from 1990 to 2021 and identify the quantitative characteristics of the research in this period. The results from the analysis revealed that the number of accumulated publications followed a quadratic growth, which confirmed the relevance this research topic is gaining during the last years. High research efforts have been invested to define safe selenium content in drinking water, since the insufficient or excessive intake of selenium and the corresponding effects on human health are only separated by a narrow margin. Some important research features of the four main technologies most frequently used to remove selenium from drinking water (coagulation, flocculation and precipitation followed by filtration; adsorption and ion exchange; membrane-based processes and biological treatments) were compiled in this work. Although the search of technological options to remove selenium from drinking water is less intensive than the search of solutions to reduce and eliminate the presence of other pollutants, adsorption was the alternative that has received the most attention according to the research trends during the studied period, followed by membrane technologies, while biological methods require further research efforts to promote their implementation.
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Affiliation(s)
- Ricardo Abejón
- Departamento de Ingeniería Química, Universidad de Santiago de Chile (USACH), Av. Libertador Bernardo O'Higgins 3363, Estación Central, Santiago 9170019, Chile
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Sharma P, Nanda K, Yadav M, Shukla A, Srivastava SK, Kumar S, Singh SP. Remediation of noxious wastewater using nanohybrid adsorbent for preventing water pollution. CHEMOSPHERE 2022; 292:133380. [PMID: 34953871 DOI: 10.1016/j.chemosphere.2021.133380] [Citation(s) in RCA: 3] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 09/28/2021] [Revised: 12/13/2021] [Accepted: 12/18/2021] [Indexed: 06/14/2023]
Abstract
Removal of toxic elements from wastewater effluent has got a lot of attention because of their severe negative effects on human and environmental health. In the past few years, rapid urbanization and industrial activities in developing countries have exacerbated the destruction of the environment. Most of the wastewater effluents are discharged untreated or inadequately treated, which has become a major concern due to its impact on sustainability and the environment. This is imperative to implement, innovative and resourceful wastewater treatment technologies requiring low investment. Among the various treatment technologies, cutting-edge processes in nano-material sciences have recently piqued the interest of scientists. Nanohybrid absorbents have the potential in improving wastewater treatment and increase water supply by utilizing unconventional water resources. Carbon nanotubes, titanium oxide, manganese oxide, activated carbon (AC), magnesium oxide, graphene, ferric oxides, and zinc oxide are examples of nano-adsorbents that are used to eliminate pollutants. This also demonstrated the effective removal of contaminants along with the harmful effects of chemicals, colorants, and metals found in wastewater. The present manuscript examines potential advances in nanotechnology in wastewater treatment for the prevention of water and soil pollution. This systematic review aims to highlight the importance of nanohybrid absorbents treatment technology for wastewater treatment and to explain how nanohybrid absorbents have the potential to revolutionize industrial pollution. There are also other published review articles on this topic but the present review covers an in-depth information on nano-adsorbents and their targeted contaminants.
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Affiliation(s)
- Pooja Sharma
- CSIR-National Environmental Engineering Research Institute (CSIR-NEERI), Nagpur, 440 020, India.
| | - Kavita Nanda
- Plant Molecular Biology Laboratory, Department of Botany, Dayanand Anglo-Vedic (PG) College, Chhatrapati Shahu Ji Maharaj University, Kanpur, 208 001, India
| | - Mamta Yadav
- Plant Molecular Biology Laboratory, Department of Botany, Dayanand Anglo-Vedic (PG) College, Chhatrapati Shahu Ji Maharaj University, Kanpur, 208 001, India
| | - Ashutosh Shukla
- Plant Molecular Biology Laboratory, Department of Botany, Dayanand Anglo-Vedic (PG) College, Chhatrapati Shahu Ji Maharaj University, Kanpur, 208 001, India
| | - Sudhir Kumar Srivastava
- Chemical Research Laboratory, Department of Chemistry, Dayanand Anglo-Vedic (PG) College, Chhatrapati Shahu Ji Maharaj University, Kanpur, 208001, India
| | - Sunil Kumar
- CSIR-National Environmental Engineering Research Institute (CSIR-NEERI), Nagpur, 440 020, India.
| | - Surendra Pratap Singh
- Plant Molecular Biology Laboratory, Department of Botany, Dayanand Anglo-Vedic (PG) College, Chhatrapati Shahu Ji Maharaj University, Kanpur, 208 001, India.
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20
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Mani MS, Dsouza VL, Dsouza HS. Evaluation of divalent metal transporter 1 (DMT1) (rs224589) polymorphism on blood lead levels of occupationally exposed individuals. Toxicol Lett 2021; 353:13-19. [PMID: 34626817 DOI: 10.1016/j.toxlet.2021.10.002] [Citation(s) in RCA: 2] [Impact Index Per Article: 0.5] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/29/2021] [Revised: 08/15/2021] [Accepted: 10/04/2021] [Indexed: 01/28/2023]
Abstract
Lead (Pb) is an environmental and public health toxicant. It affects various organ systems of the body, thereby disrupting their normal functions. To date, several genes that are known to influence the mechanism of action of lead and toxicity have been studied. Among them, the iron transporter gene, SLC11A2 (Solute Carrier 11 group A member 2) which codes for the transmembrane protein, DMT1 (Divalent Metal Transporter 1) has shown to transport other metals including zinc, copper, and lead. We investigated the influence of DMT1 polymorphism (rs224589) on blood lead (Pb-B) levels. In the present study, we enrolled 113 lead-exposed workers and performed a comprehensive biochemical analysis and genetic composition. The frequency of DMT1 variants observed in the total subjects (n = 113) was 42 % for homozygous CC wild type, 54 % for heterozygous CA, and 4 % for homozygous AA mutant. The heterozygous CA carriers presented higher Pb-B levels compared to wild type CC and mutant AA carriers. Further, a negative association was observed between Pb-B levels and hemoglobin in heterozygous CA carriers. Hence, C allele may be the risk allele that contributes to increased susceptibility to high Pb-B retention, and genotyping of DMT1 in lead exposed subjects might be used as a prognostic marker to impede organ damage due to lead toxicity.
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Affiliation(s)
- Monica Shirley Mani
- Department of Radiation Biology and Toxicology, Manipal School of Life Sciences, Manipal Academy of Higher Education, Manipal, Karnataka, India.
| | - Venzil Lavie Dsouza
- Department of Radiation Biology and Toxicology, Manipal School of Life Sciences, Manipal Academy of Higher Education, Manipal, Karnataka, India.
| | - Herman Sunil Dsouza
- Department of Radiation Biology and Toxicology, Manipal School of Life Sciences, Manipal Academy of Higher Education, Manipal, Karnataka, India.
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Childebayeva A, Goodrich JM, Chesterman N, Leon-Velarde F, Rivera-Ch M, Kiyamu M, Brutsaert TD, Bigham AW, Dolinoy DC. Blood lead levels in Peruvian adults are associated with proximity to mining and DNA methylation. ENVIRONMENT INTERNATIONAL 2021; 155:106587. [PMID: 33940396 PMCID: PMC9903334 DOI: 10.1016/j.envint.2021.106587] [Citation(s) in RCA: 7] [Impact Index Per Article: 1.8] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Subscribe] [Scholar Register] [Received: 12/15/2020] [Revised: 04/12/2021] [Accepted: 04/13/2021] [Indexed: 06/05/2023]
Abstract
BACKGROUND Inorganic lead (Pb) is common in the environment, and is toxic to neurological, renal, and cardiovascular systems. Pb exposure influences the epigenome with documented effects on DNA methylation (DNAm). We assessed the impact of low levels of Pb exposure on DNAm among non-miner individuals from two locations in Peru: Lima, the capital, and Cerro de Pasco, a highland mining town, to study the effects of Pb exposure on physiological outcomes and DNAm. METHODS Pb levels were measured in whole blood (n = 305). Blood leukocyte DNAm was determined for 90 DNA samples using the Illumina MethylationEPIC chip. An epigenome-wide association study was performed to assess the relationship between Pb and DNAm. RESULTS Individuals from Cerro de Pasco had higher Pb than individuals from Lima (p-value = 2.00E-16). Males had higher Pb than females (p-value = 2.36E-04). Pb was positively associated with hemoglobin (p-value = 8.60E-04). In Cerro de Pasco, blood Pb decreased with the distance from the mine (p-value = 0.04), and association with soil Pb was approaching significance (p-value = 0.08). We identified differentially methylated positions (DMPs) associated with genes SOX18, ZMIZ1, and KDM1A linked to neurological function. We also found 45 differentially methylated regions (DMRs), seven of which were associated with genes involved in metal ion binding and nine to neurological function and development. CONCLUSIONS Our results demonstrate that even low levels of Pb can have a significant impact on the body including changes to DNAm. We report associations between Pb and hemoglobin, Pb and distance from mining, and between blood and soil Pb. We also report associations between loci- and region-specific DNAm and Pb.
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Affiliation(s)
- Ainash Childebayeva
- Department of Anthropology, University of Michigan, Ann Arbor, MI 48109, USA; Department of Environmental Health Sciences, School of Public Health, University of Michigan, Ann Arbor, MI 48109, USA; Department of Archaeogenetics, Max Planck Institute for the Science of Human History, Jena 07745, Germany.
| | - Jaclyn M Goodrich
- Department of Environmental Health Sciences, School of Public Health, University of Michigan, Ann Arbor, MI 48109, USA
| | - Nathan Chesterman
- School for Environment and Sustainability, University of Michigan, Ann Arbor, MI 48109, USA
| | - Fabiola Leon-Velarde
- Departamento de Ciencias Biológicas y Fisiológicas, Facultad de Ciencias y Filosofía, Universidad Peruana Cayetano Heredia, Lima, Peru
| | - Maria Rivera-Ch
- Departamento de Ciencias Biológicas y Fisiológicas, Facultad de Ciencias y Filosofía, Universidad Peruana Cayetano Heredia, Lima, Peru
| | - Melisa Kiyamu
- Departamento de Ciencias Biológicas y Fisiológicas, Facultad de Ciencias y Filosofía, Universidad Peruana Cayetano Heredia, Lima, Peru
| | - Tom D Brutsaert
- Department of Exercise Science, Syracuse University, Syracuse, NY 13244, USA
| | - Abigail W Bigham
- Department of Anthropology, University of California, Los Angeles, CA 90095, USA
| | - Dana C Dolinoy
- Department of Environmental Health Sciences, School of Public Health, University of Michigan, Ann Arbor, MI 48109, USA; Department of Nutritional Sciences, School of Public Health, University of Michigan, Ann Arbor, MI 48109, USA
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22
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Makena W, Otong ES, Dibal NI, Ishaku B, Bazabang SA. Aqueous fruit pulp extract of Adansonia digitata (L) protects against lead-acetate-induced hepato-renal damage in rat model. BENI-SUEF UNIVERSITY JOURNAL OF BASIC AND APPLIED SCIENCES 2021. [DOI: 10.1186/s43088-021-00151-6] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.3] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/10/2022] Open
Abstract
Abstract
Background
Adansonia digitata (L) fruit has a multi-purpose function one among many, is the antioxidant activities of the fruit by preventing oxidative stress. The effect of Adansonia digitata (L) fruit on lead-induced liver and kidney damage is not clear. Hence, the study was aimed to assessed the protective role of Adansonia digitata (L) fruits against lead acetate induced changes in the liver and kidney function test parameters and the histology of both organ in experimental rats. The rats were divided into five groups with five rats each. All the rats were administered with respective assigned treatment once daily for 6 weeks. Rats in groups I were administered with just distil water (2 ml/kg). Rats in groups II were administered with lead acetate (30 mg/kg) while rats in groups III–V were administered Adansonia digitatata (L) fruit extract (250 mg/kg and 500 mg/kg) and Succimer (5 mg/kg) respectively, then additionally challenged with lead acetate (30 mg/kg) immediately after. At the end of the administration, the blood serum from the experimental rats were used for biochemical analysis. Then, the the organs such as the liver and kidney collected for histological study.
Results
Rats administered with Lead acetate showed an increase in AST, ALP and ALT as well as increase in urea and creatinine level (p < 0.001), when compared with the control group (group I), where as Adansonia digitatata (L) fruit prevented the effect (upsurge of serum, Urea, Creatinine, AST, ALP and ALT) of lead acetate. Rats administer with only Lead acetate revealed marked liver steatosis and the degeneration of the kidney glomerulus. The Adansonia digitatata (L) fruit extract and Succimer prevented the histological liver steatosis, as well as the degeneration of the glomerulus of the kidney cytoarchitecture.
Conclusion
The findings in this study suggest that Adansonia digitata fruits extract has a protective potentials against lead acetate induced liver and kidney toxicity by preventing the upsurge of liver function enzymes and kidney function parameters. Hence, Adansonia digitata fruits can serve as a natural plant agent that can prevent hepato-renal toxicity. Therefore, Adansonia digitata holds future prospects in preclinical framework to ameliorate organs toxicity for oral therapeutic applications.
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Hussein ABA, Ahmed MH, Kamal MM, Ayesh NAEA, Fouad MM. Evaluation of the Role of KIM-1 in Detecting Early Nephrotoxicity in Lead-Exposed Workers. J Occup Environ Med 2021; 63:e605-e611. [PMID: 34491970 DOI: 10.1097/jom.0000000000002304] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/26/2022]
Abstract
OBJECTIVES Lead nephropathy usually starts silent. This study aimed to evaluate using kidney injury molecule 1 (KIM-1) as an early nephrotoxicity predictor of long-term low-level occupational lead exposure. METHODS History, examination, and laboratory investigations including: blood lead, urinary KIM-1, serum uric acid, creatinine, urea, sodium, potassium, serum albumin, and urine analysis were done on 35 lead-exposed workers and a matched control group. RESULTS Higher blood lead levels were found among the exposed group compared to the control one. No statistically significant difference was found regarding renal failure manifestations or standard renal functions (uric acid, blood urea, and creatinine). Urinary KIM-1 was statistically significantly increased among the exposed group. CONCLUSION Renal adverse effects were associated to lead fumes exposure. KIM-1 can be used as biomarker for detecting early renal affection among lead-exposed workers.
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Affiliation(s)
- Abo-Bakr Abbas Hussein
- Department of Occupational & Environmental Medicine, Faculty of Medicine, Cairo University, Egypt (Dr Hussein, Dr Ahmed, Dr Ayesh, and Dr Fouad); Department of Clinical & Chemical Pathology, Faculty of Medicine, Cairo University, Egypt (Dr Kamal)
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24
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Hassanen EI, Khalaf AAA, Zaki AR, Ibrahim MA, Galal MK, Farroh KY, Azouz RA. Ameliorative effect of ZnO-NPs against bioaggregation and systemic toxicity of lead oxide in some organs of albino rats. ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH INTERNATIONAL 2021; 28:37940-37952. [PMID: 33723775 DOI: 10.1007/s11356-021-13399-3] [Citation(s) in RCA: 4] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 11/08/2020] [Accepted: 03/08/2021] [Indexed: 06/12/2023]
Abstract
Lead is one of the major environmental pollutions worldwide, particularly in developing countries. Though, various occupational and public health measures have been undertaken to control lead exposure. The present study is designed to investigate the role of zinc oxide nanoparticles (ZnO-NPs) to reduce the bioaggregation of lead in the brain, liver, and kidneys and prevent these organ oxidative damage and apoptosis. Twenty male Wistar rats were grouped into 4 gatherings and exposed to the following materials daily on the skin for 2 weeks: 1-normal saline, 2-ZnO-NPs, 3-PbO, and 4-ZnO-NPs+ PbO. Topical application of PbO to rats increased lead contents in blood and different organs causing remarkable oxidative stress damage, apoptosis, and histopathological alterations in these organs. Moreover, PbO-receiving group showed strong positive caspase-3 protein expression with up-regulation of mRNA levels of BAX and COX-2. Co-treatment of ZnO-NPs with PbO could diminish the toxicologic parameters and the above-mentioned immune marker and gene expression levels. Our data suggest the role of ZnO-NPs cream to reduce the risk of lead dermal exposure via preventing absorption and accumulation of it in the internal organs so that it protects these organs from further damage.
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Affiliation(s)
- Eman I Hassanen
- Department of Pathology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.
| | - Abdel-Azem A Khalaf
- Department of Toxicology& Forensic Medicine, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt
| | - Amr R Zaki
- Department of Forensic Medicine and Clinical Toxicology, Faculty of Medicine, Beni-Suef University, Beni-Suef, Egypt
| | - Marwa A Ibrahim
- Department of Biochemistry, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt
| | - Mona K Galal
- Department of Biochemistry, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt
| | - Khaled Y Farroh
- Nanotechnology & Advanced Materials Central Lab, Agricultural Research Center, Giza, Egypt
| | - Rehab A Azouz
- Department of Toxicology& Forensic Medicine, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt
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Wang L, Li X, Zhang G, Zhao H. Transcriptomic analysis of lead-induced hepatoxicology in female Japanese quails (Coturnix japonica): Implications of triglyceride synthesis, degradation and transport disruption. Comp Biochem Physiol C Toxicol Pharmacol 2021; 244:109024. [PMID: 33631343 DOI: 10.1016/j.cbpc.2021.109024] [Citation(s) in RCA: 8] [Impact Index Per Article: 2.0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 11/11/2020] [Revised: 02/13/2021] [Accepted: 02/16/2021] [Indexed: 02/07/2023]
Abstract
Lead (Pb) pollution poses great threats to mammals including human and it is also hazardous to bird life. In this study, RNA sequencing analysis was employed to examine the molecular responses to lead exposure in the liver of a toxicological model species Japanese quails (Coturnix japonica). Female birds were exposed to 0, 50, 500 and 1000 ppm waterborne Pb for 49 days. The results showed that hepatic microstructure was damaged under lead exposure featured by sinusoids dilation and irregularity as well as cell necrosis. Moreover, ultrastructural injury in the liver including mitochondrial swelling and vacuolization as well as nuclear deformation was induced by lead exposure. Lead exposure also caused the decrease of lipid droplets in the liver by oil red O staining. In addition, liver transcriptomic analysis revealed that molecular signaling and functional pathways were disrupted by lead exposure. Meanwhile, the expression of genes involved with hepatic glycerophospholipids metabolism of triglyceride synthesis and lipid transport of triglyceride transfer was significantly down-regulated by lead exposure. Moreover, the up-regulation of genes associated with fatty acid oxidation and the down-regulation of genes related with fatty acid synthesis were caused by lead exposure. The present study implied that lead induced liver malfunction and bird health risks through histopathological damages, molecular signaling disruption, genetic expression alteration and triglyceride metabolism disturbance.
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Affiliation(s)
- Ling Wang
- College of Life Sciences, Shaanxi Normal University, Xi'an 710119 No. 620, West Chang'an Avenue, Chang'an District, Xi'an, Shaanxi 710119, China
| | - Xuan Li
- College of Life Sciences, Shaanxi Normal University, Xi'an 710119 No. 620, West Chang'an Avenue, Chang'an District, Xi'an, Shaanxi 710119, China
| | - Gaixia Zhang
- College of Life Sciences, Shaanxi Normal University, Xi'an 710119 No. 620, West Chang'an Avenue, Chang'an District, Xi'an, Shaanxi 710119, China
| | - Hongfeng Zhao
- College of Life Sciences, Shaanxi Normal University, Xi'an 710119 No. 620, West Chang'an Avenue, Chang'an District, Xi'an, Shaanxi 710119, China.
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El-Bahr SM, Elbakery AM, El-Gazzar N, Amin AA, Al-Sultan S, Alfattah MA, Shousha S, Alhojaily S, Shathele M, Sabeq II, Hamouda AF. Biosynthesized Iron Oxide Nanoparticles from Petroselinum crispum Leaf Extract Mitigate Lead-Acetate-Induced Anemia in Male Albino Rats: Hematological, Biochemical and Histopathological Features. TOXICS 2021; 9:toxics9060123. [PMID: 34072696 PMCID: PMC8227184 DOI: 10.3390/toxics9060123] [Citation(s) in RCA: 10] [Impact Index Per Article: 2.5] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 03/27/2021] [Revised: 05/05/2021] [Accepted: 05/07/2021] [Indexed: 12/27/2022]
Abstract
This study aimed to investigate the ameliorative effects of iron oxide nanoparticles (IONPs) prepared from leaf extract of Petroselinum crispum compared to those prepared using a chemical method in lead-acetate-induced anemic rats. Twenty rats were divided into four groups (five rats each). Throughout the experimental period (8 weeks), the rats in group 1 were not given any therapy. The rats in groups 2, 3 and 4 were given 400 ppm lead acetate orally for 2 weeks to make them anemic. Following that, these rats were either left untreated, given 27 ppm of chemical IONPs orally or given 27 ppm of natural IONPs orally for the remaining 6 weeks of the experiment. TEM analysis indicated that the chemically and naturally prepared IONPs had sizes of 6.22–9.7 and 64–68 nm, respectively. Serum ferritin and iron concentrations were reduced, whereas the total iron-binding capacity (TIBC), ALT, AST, urea and creatinine were significantly increased in the non-treated lead-acetate-induced anemic rats compared to those of the control. In addition, congestion, hemorrhage, necrosis, vacuolation and leukocytic infiltration in the kidneys, liver and spleen were observed in non-treated lead-acetate-induced anemic rats compared to the control. The effects of lead acetate were mitigated by IONPs, particularly the natural one. In conclusion, IONPs produced from Petroselinum crispum leaf extract can be used as an efficient and safe therapy in lead-acetate-induced anemic rats.
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Affiliation(s)
- Sabry M. El-Bahr
- Department of Biomedical Sciences, College of Veterinary Medicine, King Faisal University, Al-Ahsa 31982, Saudi Arabia; (S.S.); (S.A.)
- Department of Biochemistry, Faculty of Veterinary Medicine, Alexandria University, Alexandria 21523, Egypt
- Correspondence: or
| | - Amal M. Elbakery
- Department of Maize and Sugar Crop Disease Research, Plant Pathology Research Institute, ARC, Giza 12622, Egypt;
| | - Nashwa El-Gazzar
- Department of Botany and Microbiology, Faculty of Science, Zagazig University, Zagazig 44519, Egypt;
| | - Aziza A. Amin
- Department of Histopathology, Faculty of Veterinary Medicine, Benha University, Benha 13736, Egypt;
| | - Saad Al-Sultan
- Department of Public Health, College of Veterinary Medicine, King Faisal University, Al-Ahsa 31982, Saudi Arabia;
| | | | - Saad Shousha
- Department of Biomedical Sciences, College of Veterinary Medicine, King Faisal University, Al-Ahsa 31982, Saudi Arabia; (S.S.); (S.A.)
- Department of Physiology, Faculty of Veterinary Medicine, Benha University, Benha 13736, Egypt
| | - Sameer Alhojaily
- Department of Biomedical Sciences, College of Veterinary Medicine, King Faisal University, Al-Ahsa 31982, Saudi Arabia; (S.S.); (S.A.)
| | - Mohammad Shathele
- Department of Microbiology, College of Veterinary Medicine, King Faisal University, Al-Ahsa 31982, Saudi Arabia;
| | - Islam I. Sabeq
- Department of Food Control and Hygiene, Faculty of Veterinary Medicine, Benha University, Benha 13736, Egypt;
| | - Ahlam F. Hamouda
- Department of Forensic Medicine and Toxicology, Teaching Hospital, Faculty of Veterinary Medicine, Benha University, Benha 13736, Egypt;
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Pugliese M, Biondi V, Gugliandolo E, Licata P, Peritore AF, Crupi R, Passantino A. D-Penicillamine: The State of the Art in Humans and in Dogs from a Pharmacological and Regulatory Perspective. Antibiotics (Basel) 2021; 10:antibiotics10060648. [PMID: 34071639 PMCID: PMC8229433 DOI: 10.3390/antibiotics10060648] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/30/2021] [Revised: 05/18/2021] [Accepted: 05/26/2021] [Indexed: 11/16/2022] Open
Abstract
Chelant agents are the mainstay of treatment in copper-associated hepatitis in humans, where D-penicillamine is the chelant agent of first choice. In veterinary medicine, the use of D-penicillamine has increased with the recent recognition of copper-associated hepatopathies that occur in several breeds of dogs. Although the different regulatory authorities in the world (United States Food and Drugs Administration-U.S. FDA, European Medicines Agency-EMEA, etc.) do not approve D-penicillamine for use in dogs, it has been used to treat copper-associated hepatitis in dogs since the 1970s, and is prescribed legally by veterinarians as an extra-label drug to treat this disease and alleviate suffering. The present study aims to: (a) address the pharmacological features; (b) outline the clinical scenario underlying the increased interest in D-penicillamine by overviewing the evolution of its main therapeutic goals in humans and dogs; and finally, (c) provide a discussion on its use and prescription in veterinary medicine from a regulatory perspective.
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Affiliation(s)
- Michela Pugliese
- Department of Veterinary Science, University of Messina, 98166 Messina, Italy; (M.P.); (V.B.); (E.G.); (P.L.); (A.P.)
| | - Vito Biondi
- Department of Veterinary Science, University of Messina, 98166 Messina, Italy; (M.P.); (V.B.); (E.G.); (P.L.); (A.P.)
| | - Enrico Gugliandolo
- Department of Veterinary Science, University of Messina, 98166 Messina, Italy; (M.P.); (V.B.); (E.G.); (P.L.); (A.P.)
| | - Patrizia Licata
- Department of Veterinary Science, University of Messina, 98166 Messina, Italy; (M.P.); (V.B.); (E.G.); (P.L.); (A.P.)
| | - Alessio Filippo Peritore
- Department of Chemical, Biological, Pharmaceutical, and Environmental Science, University of Messina, 98168 Messina, Italy;
| | - Rosalia Crupi
- Department of Veterinary Science, University of Messina, 98166 Messina, Italy; (M.P.); (V.B.); (E.G.); (P.L.); (A.P.)
- Correspondence:
| | - Annamaria Passantino
- Department of Veterinary Science, University of Messina, 98166 Messina, Italy; (M.P.); (V.B.); (E.G.); (P.L.); (A.P.)
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Arsenate-Induced Changes in Bacterial Metabolite and Lipid Pools during Phosphate Stress. Appl Environ Microbiol 2021; 87:AEM.02261-20. [PMID: 33361371 DOI: 10.1128/aem.02261-20] [Citation(s) in RCA: 2] [Impact Index Per Article: 0.5] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/17/2020] [Accepted: 12/06/2020] [Indexed: 11/20/2022] Open
Abstract
Agrobacterium tumefaciens GW4 is a heterotrophic arsenite-oxidizing bacterium with a high resistance to arsenic toxicity. It is now a model organism for studying the processes of arsenic detoxification and utilization. Previously, we demonstrated that under low-phosphate conditions, arsenate [As(V)] could enhance bacterial growth and be incorporated into biomolecules, including lipids. While the basic microbial As(V) resistance mechanisms have been characterized, global metabolic responses under low phosphate remain largely unknown. In the present work, the impacts of As(V) and low phosphate on intracellular metabolite and lipid profiles of GW4 were quantified using liquid chromatography-mass spectroscopy (LC-MS) in combination with transcriptional assays and the analysis of intracellular ATP and NADH levels. Metabolite profiling revealed that oxidative stress response pathways were altered and suggested an increase in DNA repair. Changes in metabolite levels in the tricarboxylic acid (TCA) cycle along with increased ATP are consistent with As(V)-enhanced growth of A. tumefaciens GW4. Lipidomics analysis revealed that most glycerophospholipids decreased in abundance when As(V) was available. However, several glycerolipid classes increased, an outcome that is consistent with maximizing growth via a phosphate-sparing phenotype. Differentially regulated lipids included phosphotidylcholine and lysophospholipids, which have not been previously reported in A. tumefaciens The metabolites and lipids identified in this study deepen our understanding of the interplay between phosphate and arsenate on chemical and metabolic levels.IMPORTANCE Arsenic is widespread in the environment and is one of the most ubiquitous environmental pollutants. Parodoxically, the growth of certain bacteria is enhanced by arsenic when phosphate is limited. Arsenate and phosphate are chemically similar, and this behavior is believed to represent a phosphate-sparing phenotype in which arsenate is used in place of phosphate in certain biomolecules. The research presented here uses a global approach to track metabolic changes in an environmentally relevant bacterium during exposure to arsenate when phosphate is low. Our findings are relevant for understanding the environmental fate of arsenic as well as how human-associated microbiomes respond to this common toxin.
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Low-level Eexposure to lead dust in unusual work schedules and hematologic, renal, and hepatic parameters. Toxicol Appl Pharmacol 2021; 415:115448. [PMID: 33577916 DOI: 10.1016/j.taap.2021.115448] [Citation(s) in RCA: 2] [Impact Index Per Article: 0.5] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 12/12/2020] [Revised: 01/27/2021] [Accepted: 02/07/2021] [Indexed: 11/22/2022]
Abstract
BACKGROUND Many workers are exposed to lead dust in lead-‑zinc mines. Exposure to this heavy toxic metal and its compounds can cause irreversible adverse health effects. OBJECTIVE To assess possible hematotoxic, nephrotoxic, and hepatotoxic potentials of low levels of lead in a group of mine workers exposed to this heavy metal in an unusual work schedule. METHODS A total of 73 exposed and 70 non-exposed employees were interviewed. Demographic data, and occupational and medical history of the employees were obtained by questionnaires. Air monitoring was performed to determine the workers' time-weighted average (TWA) exposure to lead dust. The threshold limit value (TLV) for lead was adjusted for unusual work schedules according to the model developed by the University of Montreal and the Institute de Recherche en Sante et en Securite du Travail (IRSST). Blood samples were collected for complete blood count, liver and kidney function tests. Data were analyzed using version 21.0 of the SPSS software. RESULTS The TWA exposure of workers was 24 μg/m3. On average, the worker's exposure to lead dust did not exceed the 8-h OSHA and ACGIH TLV-TWA of 50 μg/m3. Significant associations were found only between exposure to lead and platelet count, red cell distribution width, total protein, and albumin. CONCLUSIONS Exposure to low levels of lead dust in unusual work schedules was not associated with overt hematotoxicity, hepatotoxicity or nephrotoxicity. However, mild, sub-clinical, pre-pathologic significant changes were noted in some blood parameters of the exposed employees as compared with their referent counterparts.
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Ahmed MF, Mokhtar MB, Alam L. Carcinogenic and non-carcinogenic health risk of arsenic ingestion via drinking water in Langat River Basin, Malaysia. ENVIRONMENTAL GEOCHEMISTRY AND HEALTH 2021; 43:897-914. [PMID: 32372251 DOI: 10.1007/s10653-020-00571-w] [Citation(s) in RCA: 14] [Impact Index Per Article: 3.5] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 09/09/2019] [Accepted: 04/10/2020] [Indexed: 05/25/2023]
Abstract
The prolonged persistence of toxic arsenic (As) in environment is due to its non-biodegradable characteristic. Meanwhile, several studies have reported higher concentrations of As in Langat River. However, it is the first study in Langat River Basin, Malaysia, that As concentrations in drinking water supply chain were determined simultaneously to predict the health risks of As ingestion. Water samples collected in 2015 from the four stages of drinking water supply chain were analysed for As concentration by inductively coupled plasma mass spectrometry. Determined As concentrations along with the time series data (2004-2015) were significantly within the maximum limit 0.01 mg/L of drinking water quality standard set by World Health Organization. The predicted As concentration by auto-regression moving average was 3.45E-03 mg/L in 2020 at 95% level based on time series data including climatic control variables. Long-term As ingestion via household filtration water at Langat Basin showed no potential lifetime cancer risk (LCR) 9.7E-06 (t = 6.68; p = 3.37E-08) as well as non-carcinogenic hazard quotient (HQ) 4.8E-02 (t = 6.68; p = 3.37E-08) risk at 95% level. However, the changing landscape, ex-mining ponds and extensive use of pesticides for palm oil plantation at Langat Basin are considered as the major sources of increased As concentration in Langat River. Therefore, a two-layer water filtration system at Langat Basin should be introduced to accelerate the achievement of sustainable development goal of getting safe drinking water supply.
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Affiliation(s)
- Minhaz Farid Ahmed
- Institute for Environment and Development (LESTARI), Universiti Kebangsaan Malaysia (UKM), 43600, Bangi, Selangor Darul Ehsan, Malaysia
| | - Mazlin Bin Mokhtar
- Institute for Environment and Development (LESTARI), Universiti Kebangsaan Malaysia (UKM), 43600, Bangi, Selangor Darul Ehsan, Malaysia.
| | - Lubna Alam
- Institute for Environment and Development (LESTARI), Universiti Kebangsaan Malaysia (UKM), 43600, Bangi, Selangor Darul Ehsan, Malaysia
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Oyem JC, Chris-Ozoko LE, Enaohwo MT, Otabor FO, Okudayo VA, Udi OA. Antioxidative properties of Ocimum gratissimum alters Lead acetate induced oxidative damage in lymphoid tissues and hematological parameters of adult Wistar rats. Toxicol Rep 2021; 8:215-222. [PMID: 33511038 PMCID: PMC7817492 DOI: 10.1016/j.toxrep.2021.01.003] [Citation(s) in RCA: 12] [Impact Index Per Article: 3.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 05/15/2020] [Revised: 12/11/2020] [Accepted: 01/04/2021] [Indexed: 11/18/2022] Open
Abstract
Chronic lead toxicity was induced in male rats by oral administration of lead acetate. Effect of Ocimum gratissimum in lead acetate toxicity on spleen, thymus, and blood. Oral lead acetate administration led to oxidative damage in spleen, thymus and blood. Ocimum gratissimum extract reversed oxidative stress and enhanced antioxidant enzymes. Ocimum gratissimum averts lead acetate-induced toxicity in the blood, thymus and spleen. Lead exposure is a well-known environmental hazard. Its accumulation in humans may pose a danger to health. The present study investigated the beneficial effect of Ocimum gratissimum extract (OG) in reducing lead acetate (LA) induced oxidative damage in the spleen, thymus, and hematological indices. We employed an in vivo model of LA induced Wistar rats and administered 125 mg/kg/bw and 250 mg/kg/bw of OG extracts respectively. Our control groups were divided into 2; the first group received normal saline, feed, and water while the second group was administered OG extracts only. We assessed the levels of malondialdehyde (MDA), glutathione (GSH), superoxide dismutase (SOD), and catalase (CAT) in the thymus and spleen and estimated percentages of blood cells. Our results showed that LA induces oxidative damage by significantly elevating MDA and diminishing GSH levels, SOD, and CAT activities. LA administration led to a significant decline in blood parameters. However, co-administration with OG compensated oxidative stress by significantly reducing MDA, increasing GSH, SOD, and CAT. Oral administration of OG to rats attenuated anemia, thrombocytopenia, leucocytosis, eosinophilia, monocytosis, and neutropenia induced by LA. The present study indicates that LA induced Spleen, thymus, and blood toxicity, which was reversed by oral OG administration.
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Key Words
- Antioxidation
- BW, Bodyweight
- CAT, Catalase
- GSH, Glutathione
- H2O2, Hydrogen peroxide
- Hb, Hemaglobin
- LA, Lead Acetate
- Lead acetate
- MDA, malondialdehyde
- OG, Ocimum gratissimum
- Ocimum gratissimum
- Oxidative stress
- RBC, Red Blood Cell
- ROS, Reactive Oxygen Species
- SOD, Superoxide Dismustase
- Spleen
- Thymus
- WBC, White Blood Cell
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Affiliation(s)
- John Chukwuma Oyem
- Department of Human Anatomy and Cell Biology, Faculty of Basic Medical Sciences, Delta State University, Abraka, Nigeria
| | - Lilian Ebite Chris-Ozoko
- Department of Human Anatomy and Cell Biology, Faculty of Basic Medical Sciences, Delta State University, Abraka, Nigeria
| | - Mamerhi Taniyohwo Enaohwo
- Department of Human Anatomy and Cell Biology, Faculty of Basic Medical Sciences, Delta State University, Abraka, Nigeria
| | - Francisca Osamahemwem Otabor
- Department of Human Anatomy and Cell Biology, Faculty of Basic Medical Sciences, Delta State University, Abraka, Nigeria
| | - Vera Anieze Okudayo
- Department of Human Anatomy and Cell Biology, Faculty of Basic Medical Sciences, Delta State University, Abraka, Nigeria
| | - Onoriode Andrew Udi
- Department of Basic Medical Sciences (Anatomy Unit), College of Natural and Applied Sciences, Achievers University Owo, Ondo State, Nigeria
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Hu Y, Xiao T, Zhang A. Associations between and risks of trace elements related to skin and liver damage induced by arsenic from coal burning. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 2021; 208:111719. [PMID: 33396050 DOI: 10.1016/j.ecoenv.2020.111719] [Citation(s) in RCA: 29] [Impact Index Per Article: 7.3] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 10/06/2020] [Revised: 11/21/2020] [Accepted: 11/23/2020] [Indexed: 05/10/2023]
Abstract
Long-term exposure to high levels of arsenic has been documented to induce skin and liver damage, affecting hundreds of millions of people. While arsenic-induced skin and liver damage and trace element alterations have been studied, their correlations and risks have not been explained. Based on the above premise, this study included a total of 172 subjects from a coal-burning arsenic poisoning area. The levels of 18 trace elements in hair and six liver function indices in serum were detected, and the associations between and risks of trace elements related to skin and liver damage were analyzed. Finally, the receiver operating characteristic (ROC) curve and areas under the curve (AUC) were used to analyze the diagnostic values of certain trace elements for arsenic-induced skin and liver damage. The results found that a decrease in Se was a risk factor for arsenic-induced skin and liver damage (OR = 8.33 and 1.92, respectively). Furthermore, increases in Al and V were risk factors for arsenic-induced skin damage (OR = 1.05) and liver damage (OR = 13.16), respectively. In addition, the results found that Se and Al possessed certain diagnostic values for arsenic-induced skin damage (AUC = 0.93, 0.80), that Se possessed a diagnostic value for liver damage (AUC = 0.93), and that the combination of Se and Al increased the diagnostic value for skin damage (AUC = 0.96). This study provides an important research basis for further understanding the reasons for arsenic-induced skin and liver damage, for screening and identifying candidate diagnostic biomarkers, and for improving prevention and control strategies for arsenism.
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Affiliation(s)
- Yong Hu
- Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Department of Toxicology, School of Public Health, Guizhou Medical University, Guiyang 550025, Guizhou, China
| | - Tingting Xiao
- Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Department of Toxicology, School of Public Health, Guizhou Medical University, Guiyang 550025, Guizhou, China
| | - Aihua Zhang
- Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Department of Toxicology, School of Public Health, Guizhou Medical University, Guiyang 550025, Guizhou, China.
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Wang Y, Zhao H, Mu M, Guo M, Xing M. Zinc offers splenic protection through suppressing PERK/IRE1-driven apoptosis pathway in common carp (Cyprinus carpio) under arsenic stress. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 2021; 208:111473. [PMID: 33068983 DOI: 10.1016/j.ecoenv.2020.111473] [Citation(s) in RCA: 41] [Impact Index Per Article: 10.3] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 08/03/2020] [Revised: 10/05/2020] [Accepted: 10/06/2020] [Indexed: 06/11/2023]
Abstract
Arsenic (As) occurs naturally and concentrations in water bodies can reach high levels, leading to accumulation in vital organs like the spleen. Being an important organ in immune response and blood development processes, toxic effects of As on the spleen could compromise immunity and cause associated disorders in affected individuals. Splenic detoxification is key to improving the chances of survival but relatively little is known about the mechanisms involved. Essential trace elements like zinc have shown immune-modulatory effects humans and livestock. This study aimed to investigate the mechanisms involved in As-induced splenic toxicity in the common carp (Cyprinus carpio), and the protective effects of zinc (Zn). Our findings suggest that environmental exposure to As caused severe histological injuries and Ca2+ accumulation in the spleen of common carp. Additionally, transcriptional and translational profiles of endoplasmic reticulum stress, apoptosis and autophagy-related genes of the spleen showed upward trends under As toxicity. Treatment with Zn appears to offer protection against As-induced splenic injury in common carp and the pathologic changes above were alleviated. Our results provide additional insight into the mechanism of As toxicity in common carp while elucidating the role of Zn, a natural immune-modulator, as a potential antidote against As poisoning.
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Affiliation(s)
- Yu Wang
- College of Wildlife and Protected Area, Northeast Forestry University, Harbin 150040, Heilongjiang, PR China
| | - Hongjing Zhao
- College of Wildlife and Protected Area, Northeast Forestry University, Harbin 150040, Heilongjiang, PR China
| | - Mengyao Mu
- College of Wildlife and Protected Area, Northeast Forestry University, Harbin 150040, Heilongjiang, PR China
| | - Menghao Guo
- College of Wildlife and Protected Area, Northeast Forestry University, Harbin 150040, Heilongjiang, PR China
| | - Mingwei Xing
- College of Wildlife and Protected Area, Northeast Forestry University, Harbin 150040, Heilongjiang, PR China.
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Finlay NC, Peacock CL, Hudson-Edwards KA, Johnson KL. Characteristics and mechanisms of Pb(II) sorption onto Fe-rich waste water treatment residue (WTR): A potential sustainable Pb immobilisation technology for soils. JOURNAL OF HAZARDOUS MATERIALS 2021; 402:123433. [PMID: 32683153 DOI: 10.1016/j.jhazmat.2020.123433] [Citation(s) in RCA: 3] [Impact Index Per Article: 0.8] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Subscribe] [Scholar Register] [Received: 03/30/2020] [Revised: 06/12/2020] [Accepted: 07/06/2020] [Indexed: 06/11/2023]
Abstract
Pb contamination of soils is a global problem. This paper discusses the ability of an Fe-rich waste, water treatment residual (WTR), to adsorb Pb(II). This was investigated using batch sorption experiments, X-ray diffraction, electron microprobe microanalysis, PHREEQC modeling and Extended X-ray Absorption Fine Structure (EXAFS) analysis. The WTR is composed of approximately 23 wt. % natural organic matter (NOM), 70 wt. % ferrihydrite and <10 wt. % silicate material. Pb(II) sorption to WTR was dependent on initial Pb(II) load, particle size, time and pH, but not on ionic strength. EXAFS analysis at the Pb LIII-edge confirmed that Pb(II) sorbed to WTR by co-existing bidentate edge-sharing and monodentate or corner-sharing complexes, with 2 O at ∼2.31-2.34 Å, 1 Fe at ∼3.32-3.34 Å, 2 Fe at ∼3.97-3.99 Å and 1 Pb at ∼3.82-3.85 Å. Linear combination showed that the Pb(II)-sorbed spectra were best fit with a ∼0.9 ± 0.1 and 0.1 ± 0.1 contribution from Pb(II)-sorbed ferrihydrite and Pb(II)-sorbed humic acid end members, respectively. Overall, we show that Pb(II) sorbs via strong inner-sphere complexation of Pb(II) to the ferrihydrite component of the WTR, which itself is stable over a wide pH range. Therefore, we suggest that Fe-rich WTR wastes could be used as effective adsorbents in Pb(II)-contaminated soils to help ensure sustainable terrestrial ecosystems.
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Affiliation(s)
- Nina C Finlay
- Department of Engineering, Durham University, Durham DH1 3LE, UK
| | | | - Karen A Hudson-Edwards
- Environment & Sustainability Institute and Camborne School of Mines, University of Exeter, Penryn, Cornwall, TR10 9FE, UK.
| | - Karen L Johnson
- Department of Engineering, Durham University, Durham DH1 3LE, UK
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Zhang Y, Zhang P, Shang X, Lu Y, Li Y. Exposure of lead on intestinal structural integrity and the diversity of gut microbiota of common carp. Comp Biochem Physiol C Toxicol Pharmacol 2021; 239:108877. [PMID: 32828913 DOI: 10.1016/j.cbpc.2020.108877] [Citation(s) in RCA: 35] [Impact Index Per Article: 8.8] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 07/04/2020] [Revised: 08/13/2020] [Accepted: 08/18/2020] [Indexed: 12/22/2022]
Abstract
Lead is an environmental toxicant that has toxicity effect to the health of aquatic organisms. Gut microbiota has been reported to be closely related to human health. The aim of this study was to investigate the effects of lead exposure on the composition of gut microbiota. The composition of gut microbiota alteration was detected by 16S rRNA gene sequencing, following a 42-day exposure of lead (1 mg/L). The results showed that compared with the normal control group, the carp of lead group showed severe intestinal tissues injury and decreased Zona Occludens 1 (ZO-1) and occludin expression. The production of LPS in serum was increased by the treatment of lead exposure. Our results showed gut bacterial diversity in lead-treated common carp was lower than the control group. At the phylum level, the abundance of Bacteroidetes (LPS producing bacteria) and Fusobacteria in lead-treated carp were much higher than the control carp. And the abundance of Actinobacteria decreased by lead exposure. At the genus level, we found the abundance of Bacteroides (LPS producing bacteria) and Plesiomonas (an important pathogenic bacteria), increased significantly by lead exposure. And the abundance of Akkermansia, a critical probiotics, was markedly inhibited by lead exposure. In conclusion, this study indicated exposure of carp to lead causes gut microbiota alterations and intestinal structural integrity destruction.
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Affiliation(s)
- Yue Zhang
- College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China; Ministry of Education Laboratory of Animal Production and Quality Security, Jilin Agricultural University, Changchun 130118, China
| | - Peijun Zhang
- Health Monitoring and Inspection Center of Jilin Province, Changchun 130062, China
| | - Xinchi Shang
- College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China; Ministry of Education Laboratory of Animal Production and Quality Security, Jilin Agricultural University, Changchun 130118, China
| | - Yuting Lu
- College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China; Ministry of Education Laboratory of Animal Production and Quality Security, Jilin Agricultural University, Changchun 130118, China
| | - Yuehong Li
- College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China; Ministry of Education Laboratory of Animal Production and Quality Security, Jilin Agricultural University, Changchun 130118, China.
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(Ascorb)ing Pb Neurotoxicity in the Developing Brain. Antioxidants (Basel) 2020; 9:antiox9121311. [PMID: 33371438 PMCID: PMC7767447 DOI: 10.3390/antiox9121311] [Citation(s) in RCA: 20] [Impact Index Per Article: 4.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 11/29/2020] [Revised: 12/16/2020] [Accepted: 12/17/2020] [Indexed: 12/21/2022] Open
Abstract
Lead (Pb) neurotoxicity is a major concern, particularly in children. Developmental exposure to Pb can alter neurodevelopmental trajectory and has permanent neuropathological consequences, including an increased vulnerability to further stressors. Ascorbic acid is among most researched antioxidant nutrients and has a special role in maintaining redox homeostasis in physiological and physio-pathological brain states. Furthermore, because of its capacity to chelate metal ions, ascorbic acid may particularly serve as a potent therapeutic agent in Pb poisoning. The present review first discusses the major consequences of Pb exposure in children and then proceeds to present evidence from human and animal studies for ascorbic acid as an efficient ameliorative supplemental nutrient in Pb poisoning, with a particular focus on developmental Pb neurotoxicity. In doing so, it is hoped that there is a revitalization for further research on understanding the brain functions of this essential, safe, and readily available vitamin in physiological states, as well to justify and establish it as an effective neuroprotective and modulatory factor in the pathologies of the nervous system, including developmental neuropathologies.
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Rajak C, Singh N, Parashar P. Metal toxicity and natural antidotes: prevention is better than cure. ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH INTERNATIONAL 2020; 27:43582-43598. [PMID: 32951168 DOI: 10.1007/s11356-020-10783-3] [Citation(s) in RCA: 9] [Impact Index Per Article: 1.8] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 04/18/2020] [Accepted: 09/09/2020] [Indexed: 06/11/2023]
Abstract
Toxicity due to heavy metals (HM), specifically mercury (Hg), arsenic (As), lead (Pb), and cadmium (Cd) remains a challenge to scientists till date. This review gives insights into natural antidotes for the management and prevention of HM toxicity. Various databases such as PubMed, Embase, and Science Direct were searched for available facts on natural antidotes and their commercial products against HM toxicity till date. Toxicity owing to such metals needs prevention rather than therapy. Natural antidotes, fruits and vegetables, rich in antioxidant are the answers to such toxicities. Synthetic chelators impart a major drawback of removing essential metals required for normal body function, along with the toxic one. Natural antioxidants are bestowed with scavenging and chelation properties and can be alternative for synthetic chelating agents. Natural compounds are abundantly available, economic, and have minimal side effects when compared with classical chelators. Prevention is better than cure and thus adding plentiful vegetables and fruits to our diet can combat HM toxicity-related illness. Graphical abstract.
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Affiliation(s)
- Chetan Rajak
- Department of Pharmaceutics, National Institute of Pharmaceutical Education and Research, Near CRPF Base Camp, Ahmadpur urf Kamlapur, Bijnor-Sisendi Road, Sarojini Nagar, Lucknow, Uttar Pradesh, 226002, India
| | - Neelu Singh
- Department of Pharmaceutical Sciences, Babasaheb Bhimrao Ambedkar University (A Central University), Vidya Vihar, Raebareli Road, Lucknow, UP, 226025, India
| | - Poonam Parashar
- Department of Pharmaceutics, National Institute of Pharmaceutical Education and Research, Near CRPF Base Camp, Ahmadpur urf Kamlapur, Bijnor-Sisendi Road, Sarojini Nagar, Lucknow, Uttar Pradesh, 226002, India.
- Department of Pharmaceutical Sciences, Babasaheb Bhimrao Ambedkar University (A Central University), Vidya Vihar, Raebareli Road, Lucknow, UP, 226025, India.
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Yadav AK, Sinha AK, Egnew N, Romano N, Kumar V. Potential amelioration of waterborne iron toxicity in channel catfish (Ictalurus punctatus) through dietary supplementation of vitamin C. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 2020; 205:111337. [PMID: 32979804 DOI: 10.1016/j.ecoenv.2020.111337] [Citation(s) in RCA: 7] [Impact Index Per Article: 1.4] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 07/22/2020] [Revised: 09/09/2020] [Accepted: 09/10/2020] [Indexed: 05/20/2023]
Abstract
Iron overload in water is a problem in many areas of the world, which could exert toxic effects on fish. To achieve maximum growth and overall fitness, iron induced toxicity must be alleviated. Therefore, this research was undertaken to investigate the potential mitigation of iron toxicity by dietary vitamin C supplementation in channel catfish (Ictalurus punctatus). Two doses of vitamin C (143 and 573 mg/kg diet) were tested against high environmental iron (HEI, 9.5 mg/L representing 25% of 96 h LC50). Fish were randomly divided into six groups with four replicated tanks. The groups were Control (vitamin C deficient feed), LVc (143 mg vitamin C supplemented per kg diet), HVc (573 mg vitamin C supplemented per kg diet), Con + Fe (control exposed to HEI), LVc + Fe (LVc exposed to HEI) and HVc + Fe (HVc exposed to HEI). Following an 8 week trial, there was a significant reduction in weight gain (WG%) in Con + Fe compared to the control, indicating a toxic effect of HEI on fish growth performance. Interestingly, WG% in both LVc + Fe and HVc + Fe groups were significantly higher than Cont + Fe, signifying that HEI inhibited growth, but this was alleviated by vitamin C. Both hemoglobin content and hematocrit were higher in LVc + Fe compared to the control and Con + Fe. In addition, exposure to HEI (Con + Fe) incited hepatic oxidative stress based on an over-accumulation of malondialdehyde (MDA) along with a significant inhibition in superoxide dismutase (SOD) and catalase (CAT) activities; whereas in LVc + Fe and HVc + Fe, the MDA content restored to basal level. A series of histopathological alterations were observed in the liver and gills, with the most severe lesions in Con + Fe, which was also complemented with a remarkable increase in hepatic iron accumulation. Vitamin C supplementations reduced the augmented concentrations of iron accumulation to that of the control. No effect, regardless of the treatments, was noted for fatty acid composition of muscle. Overall, our findings suggest that the vitamin C supplementation can be an effective therapeutic approach for boosting growth as well as alleviating iron toxicity in catfish.
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Affiliation(s)
- Amit K Yadav
- Aquaculture Research Institute, Department of Animal, Veterinary and Food Sciences, University of Idaho, Moscow, ID, 83844, USA
| | - Amit K Sinha
- Department of Aquaculture and Fisheries, University of Arkansas at Pine Bluff, 1200 North University Drive, Pine Bluff, 71601, AR, USA
| | - Nathan Egnew
- Department of Aquaculture and Fisheries, University of Arkansas at Pine Bluff, 1200 North University Drive, Pine Bluff, 71601, AR, USA
| | - Nicholas Romano
- Department of Aquaculture and Fisheries, University of Arkansas at Pine Bluff, 1200 North University Drive, Pine Bluff, 71601, AR, USA
| | - Vikas Kumar
- Aquaculture Research Institute, Department of Animal, Veterinary and Food Sciences, University of Idaho, Moscow, ID, 83844, USA; Hagerman Fish Culture Experiment Station, University of Idaho, 3059F National Fish Hatchery Road, Hagerman, ID, 83332, USA.
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Hu Y, Xiao T, Wang Q, Liang B, Zhang A. Effects of Essential Trace Elements and Oxidative Stress on Endemic Arsenism Caused by Coal Burning in PR China. Biol Trace Elem Res 2020; 198:25-36. [PMID: 31960276 DOI: 10.1007/s12011-020-02047-5] [Citation(s) in RCA: 16] [Impact Index Per Article: 3.2] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 11/06/2019] [Accepted: 01/15/2020] [Indexed: 12/12/2022]
Abstract
Few studies have investigated the association between essential trace elements and oxidative stress in environmental media and populations with endemic arsenism caused by coal burning. Element contents and oxidative stress indicators were measured. Moreover, the expression of genes related to the nuclear factor E2-related factor 2 (Nrf2)/Kelch-like ECH-associated protein 1 (Keap1)-antioxidant response element (ARE) signaling pathway and Nrf2-ARE binding ability is detected. The results show that the contents of arsenic, copper, iron, and chromium were increased in environmental media from the arsenism area compared with the control area; however, the selenium content decreased. The arsenic, iron, chromium, and copper contents and the copper/zinc ratio were also increased in the arsenic-exposed population; however, the selenium content decreased. The results also show that the concentrations of arsenic, iron, and chromium and the copper/zinc ratio increased gradually with the severity of arsenism. However, selenium concentrations decreased gradually with the severity of arsenism. The contents of malondialdehyde, 8-hydroxyldeoxyguanosine, and protein carbonyl in plasma increased, while the levels of sulfhydryl, thioredoxin reductase (TrxR), glutathione peroxidase (Gpx), and superoxide dismutase 1 (SOD1) decreased. The mRNA expression of Keap1 and TrxR1 decreased in the blood, while the mRNA expression of Nrf2, GPx1, and SOD1 increased. Moreover, the Nrf2 protein content and Nrf2-ARE binding ability increased, and the Keap1 protein content decreased. In conclusion, our data suggest that the increased arsenic content in environmental media and populations was accompanied by abnormal levels of essential trace elements. Insufficient selenium intake, copper, and chromium overload and a high copper/zinc ratio might be some of the causes of arsenism, which might be related to the Nrf2/Keap1-ARE signaling pathway.
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Affiliation(s)
- Yong Hu
- Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Department of Toxicology, School of Public Health, Guizhou Medical University, Guiyang, 550025, Guizhou, People's Republic of China
| | - Tingting Xiao
- Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Department of Toxicology, School of Public Health, Guizhou Medical University, Guiyang, 550025, Guizhou, People's Republic of China
| | - Qi Wang
- Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Department of Toxicology, School of Public Health, Guizhou Medical University, Guiyang, 550025, Guizhou, People's Republic of China
| | - Bing Liang
- Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Department of Toxicology, School of Public Health, Guizhou Medical University, Guiyang, 550025, Guizhou, People's Republic of China
| | - Aihua Zhang
- Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Department of Toxicology, School of Public Health, Guizhou Medical University, Guiyang, 550025, Guizhou, People's Republic of China.
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Patwa J, Flora SJS. Heavy Metal-Induced Cerebral Small Vessel Disease: Insights into Molecular Mechanisms and Possible Reversal Strategies. Int J Mol Sci 2020; 21:ijms21113862. [PMID: 32485831 PMCID: PMC7313017 DOI: 10.3390/ijms21113862] [Citation(s) in RCA: 21] [Impact Index Per Article: 4.2] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 04/28/2020] [Revised: 05/24/2020] [Accepted: 05/25/2020] [Indexed: 12/11/2022] Open
Abstract
Heavy metals are considered a continuous threat to humanity, as they cannot be eradicated. Prolonged exposure to heavy metals/metalloids in humans has been associated with several health risks, including neurodegeneration, vascular dysfunction, metabolic disorders, cancer, etc. Small blood vessels are highly vulnerable to heavy metals as they are directly exposed to the blood circulatory system, which has comparatively higher concentration of heavy metals than other organs. Cerebral small vessel disease (CSVD) is an umbrella term used to describe various pathological processes that affect the cerebral small blood vessels and is accepted as a primary contributor in associated disorders, such as dementia, cognitive disabilities, mood disorder, and ischemic, as well as a hemorrhagic stroke. In this review, we discuss the possible implication of heavy metals/metalloid exposure in CSVD and its associated disorders based on in-vitro, preclinical, and clinical evidences. We briefly discuss the CSVD, prevalence, epidemiology, and risk factors for development such as genetic, traditional, and environmental factors. Toxic effects of specific heavy metal/metalloid intoxication (As, Cd, Pb, Hg, and Cu) in the small vessel associated endothelium and vascular dysfunction too have been reviewed. An attempt has been made to highlight the possible molecular mechanism involved in the pathophysiology, such as oxidative stress, inflammatory pathway, matrix metalloproteinases (MMPs) expression, and amyloid angiopathy in the CSVD and related disorders. Finally, we discussed the role of cellular antioxidant defense enzymes to neutralize the toxic effect, and also highlighted the potential reversal strategies to combat heavy metal-induced vascular changes. In conclusion, heavy metals in small vessels are strongly associated with the development as well as the progression of CSVD. Chelation therapy may be an effective strategy to reduce the toxic metal load and the associated complications.
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Abstract
Arsenic (As) is widely used in the modern industry, especially in the production of pesticides, herbicides, wood preservatives, and semiconductors. The sources of As such as contaminated water, air, soil, but also food, can cause serious human diseases. The complex mechanism of As toxicity in the human body is associated with the generation of free radicals and the induction of oxidative damage in the cell. One effective strategy in reducing the toxic effects of As is the usage of chelating agents, which provide the formation of inert chelator–metal complexes with their further excretion from the body. This review discusses different aspects of the use of metal chelators, alone or in combination, in the treatment of As poisoning. Consideration is given to the therapeutic effect of thiol chelators such as meso-2,3-dimercaptosuccinic acid, sodium 2,3-dimercapto-1-propanesulfonate, 2,3-dimercaptopropanol, penicillamine, ethylenediaminetetraacetic acid, and other recent agents against As toxicity. The review also considers the possible role of flavonoids, trace elements, and herbal drugs as promising natural chelating and detoxifying agents.
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Moghaddam M, Mehdizadeh L, Sharifi Z. Macro- and microelement content and health risk assessment of heavy metals in various herbs of Iran. ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH INTERNATIONAL 2020; 27:12320-12331. [PMID: 31993898 DOI: 10.1007/s11356-020-07789-2] [Citation(s) in RCA: 9] [Impact Index Per Article: 1.8] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Subscribe] [Scholar Register] [Received: 07/04/2019] [Accepted: 01/21/2020] [Indexed: 06/10/2023]
Abstract
In this study, element content and health risk of the most popular herbs from Iran were evaluated. The samples of raw materials from 30 different herbs were purchased from the local markets of Iran. The concentration levels of some elements including macroelements (N, P, K, Ca, and Mg), micronutrients (Fe, Zn, Cu, Mn, and Na), and heavy metals (Cd, Ni, and Pb) of studied herbs were evaluated. The potential of health risks was calculated by Estimated Daily Intake (EDI), Target Hazard Quotient (THQ), and Hazard Index (HI). The analysis of variance (ANOVA) is used to test a hypothesis about differences between the mean values. The highest levels of Ca (20,000 ± 26.3 mg/kg), Mg (9600 ± 45.4 mg/kg), N (59,955 ± 11.55 mg/kg), P (6544 ± 20 mg/kg), and K (56,563.2 ± 18 mg/kg) were found in Zataria multiflora, Malva sylvestris, Acasia arbus, Cannabis sativa, and Amomum subulatum, respectively. In addition, the highest concentration levels of Fe (987 ± 75.27 mg/kg), Zn (1187.5 ± 10 mg/kg), Cu (64.2 ± 2 mg/kg), Mn (272.3 ± 66.62 mg/kg), and Na (2658.8 ± 20.3 mg/kg) were recorded in Bunium persicum, Peganum harmala, Papaver somniferum, Alpinia officinalis, and Cuminum cyminum, respectively. Acasia arbus, Anethum graveolens, and Malva sylvestris showed the highest concentration of Ni (6.07 ± 0.04 mg/kg), Cd (1.64 ± 0.16 mg/kg), and Pb (9.27 ± 0.25 mg/kg). Based on performed health risk assessment on the studied plants, EDI, THQ, and HI values of all of them were less than 1. This study indicated that there were several harmful elements in the herbs. The healthier plant species are those with the least concentration of Pb, Ni, and Cd, which include Vitex agnus-custus and Teucrium polium. On the other hand, the toxic plants with a higher concentration of Pb, Ni, and Cd included Malva sylvestris, Acasia arbus, and Anethum graveolens. In addition, evaluation of human risk assessment is an important factor for investigating the concentration of heavy metals harmful for human beings.
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Affiliation(s)
- Mohammad Moghaddam
- Department of Horticulture Science, Faculty of Agriculture, Ferdowsi University of Mashhad, P.O. Box 91775-1163, Mashhad, Iran.
| | - Leila Mehdizadeh
- Department of Horticulture Science, Faculty of Agriculture, Ferdowsi University of Mashhad, P.O. Box 91775-1163, Mashhad, Iran
| | - Zahra Sharifi
- Department of Soil Science, Faculty of Agriculture, Ferdowsi University of Mashhad, P.O. Box 91775-1163, Mashhad, Iran
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Eftekhar SP, Kazemi S, Moghadamnia AA. A brief review of the latest pharmacological treatments of COVID-19. CASPIAN JOURNAL OF INTERNAL MEDICINE 2020; 11:460-465. [PMID: 33425262 PMCID: PMC7780866 DOI: 10.22088/cjim.11.0.460] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Download PDF] [Subscribe] [Scholar Register] [Received: 04/15/2020] [Revised: 07/19/2020] [Accepted: 07/20/2020] [Indexed: 12/05/2022]
Abstract
New Coronavirus which is called 2019-nCoV (2019-Novel-Coronavirus) or SARS-Cov-2 (Severe Acute respiratory Syndrome-Coronavirus 2) causes deadly pneumonia that first appeared in December 2019 in Wuhan city in China. This virus spreads all over the world quickly and made several problems for the community and healthcare system. Several drugs have been tried to manage COVID-19; however, our knowledge of this virus is not complete. At any rate, effective treatment or vaccine for this disease has not been discovered yet. Furthermore, to achieve this goal, more studies are needed on the structure of the virus and its pathogenesis mechanism. In this article, we summarized several articles suggesting treatments of COVID-19.
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Affiliation(s)
| | - Sohrab Kazemi
- Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol Iran
| | - Ali Akbar Moghadamnia
- Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol Iran
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Risk Assessment of Contamination by Potentially Toxic Metals: A Case Study in the Vicinity of an Abandoned Pyrite Mine. MINERALS 2019. [DOI: 10.3390/min9120783] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.2] [Reference Citation Analysis] [Abstract] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 11/17/2022]
Abstract
Abandoned mining areas can display soil and water pollution and also a high incidence of endemic diseases. Here, based on preliminary results on mental retardation and high incidence rates of cancers, we investigate the contamination status and potential ecological risk at an abandoned pyrite mine located in Xianju village, Hubei province, central China. The study focused on the three potentially toxic metals As, Pb, and Zn and four other common elements, including Ca, Fe, S, and Se. Soil samples were collected from 12 sites and leachate seeping from five sites. Leachates were strongly acidic compared to the soil, with pH values <3.22. Soil As and Se concentrations exceeded the Chinese Grade II standards for soil environmental quality (GB 15618-2009) (30 and 3 mg·kg−1, respectively), ranging from 33.6 ± 0.7 to 78.2 ± 1.0 mg As·kg−1 dry matter (DM) and 5.3 ± 0.8 to 17.1 ± 1.4 mg Se·kg−1 DM. Arsenic, Fe, Se, and S in leachates all exceeded the Chinese Grade III standard for surface water environmental quality (GB 3838-2002) (0.1, 0.3, 0.03, and 1.0 mg·L−1). The potential ecological risks from Pb and Zn in soils were low, but As in soils and leachates represented a moderate or strong risk to children according to the Nemerow index and hazard quotient. Soil replacement combined with further remediation measures is required to remediate the contaminated area.
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Palanirajan SK, Gummadi SN. Heavy-Metals-Mediated Phospholipids Scrambling by Human Phospholipid Scramblase 3: A Probable Role in Mitochondrial Apoptosis. Chem Res Toxicol 2019; 33:553-564. [PMID: 31769662 DOI: 10.1021/acs.chemrestox.9b00406] [Citation(s) in RCA: 3] [Impact Index Per Article: 0.5] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/20/2022]
Abstract
Human phospholipid scramblases are a family of four homologous transmembrane proteins (hPLSCR1-4) mediating phospholipids (PLs) translocation in plasma membrane upon Ca2+ activation. hPLSCR3, the only homologue localized to mitochondria, plays a vital role in mitochondrial structure, function, maintenance, and apoptosis. Upon Ca2+ activation, hPLSCR3 mediates PL translocation at the mitochondrial membrane enhancing t-bid-induced cytochrome c release and apoptosis. Mitochondria are important target organelles for heavy-metals-induced apoptotic signaling cascade and are the central executioner of apoptosis to trigger. Pb2+ and Hg2+ toxicity mediates apoptosis by increased reactive oxygen species (ROS) and cytochrome c release from mitochondria. To discover the role of hPLSCR3 in heavy metal toxicity, hPLSCR3 was overexpressed as a recombinant protein in Escherichia coli Rosetta (DE3) and purified by affinity chromatography. The biochemical assay using synthetic proteoliposomes demonstrated that hPLSCR3 translocated aminophospholipids in the presence of micromolar concentrations of Pb2+ and Hg2+. A point mutation in the Ca2+-binding motif (F258V) led to a ∼60% loss in the functional activity and decreased binding affinities for Pb2+ and Hg2+ implying that the divalent heavy metal ions bind to the Ca2+-binding motif. This was further affirmed by the characteristic spectra observed with stains-all dye. The conformational changes upon heavy metal binding were monitored by circular dichroism, intrinsic tryptophan fluorescence, and light-scattering studies. Our results revealed that Pb2+ and Hg2+ bind to hPLSCR3 with higher affinity than Ca2+ thus mediating scramblase activity. To summarize, this is the first biochemical evidence for heavy metals binding to the mitochondrial membrane protein leading to bidirectional translocation of PLs specifically toward phosphatidylethanolamine.
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Affiliation(s)
- Santosh Kumar Palanirajan
- Applied and Industrial Microbiology Laboratory, Department of Biotechnology, Bhupat and Jyoti Mehta School of Biosciences , Indian Institute of Technology Madras , Chennai 600 036 , India
| | - Sathyanarayana N Gummadi
- Applied and Industrial Microbiology Laboratory, Department of Biotechnology, Bhupat and Jyoti Mehta School of Biosciences , Indian Institute of Technology Madras , Chennai 600 036 , India
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Zhao H, Wang Y, Guo M, Fei D, Mu M, Yu H, Xing M. Hepatoprotective effects of zinc (II) via cytochrome P-450/reactive oxygen species and canonical apoptosis pathways after arsenite waterborne exposure in common carp. CHEMOSPHERE 2019; 236:124869. [PMID: 31549675 DOI: 10.1016/j.chemosphere.2019.124869] [Citation(s) in RCA: 20] [Impact Index Per Article: 3.3] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 06/05/2019] [Revised: 08/26/2019] [Accepted: 09/13/2019] [Indexed: 06/10/2023]
Abstract
Chronic arsenicosis has threatened the survival of aquatic animals with molecular mechanisms yet clear. In the present study, liver damage was evident by fluctuated activities of transaminases and declined ATPases in common carp under arsenic (As) exposure for 30 days. Mechanically, As significantly decreased cytochrome P-1A (CYP1A) activity and increased reactive oxygen species (ROS) content, which corroborated mitochondrial dysfunction in the hepatocytes. This hypothesis was further suggested by Caspase-3-executed apoptosis by death receptor pathway (Fas, TNF-α and Caspase-8) and mitochondrial pathway (Bax, Bcl-2 and Caspase-9). The above results indicated that As-elicited oxidative damage lead to apoptotic hepatic injury in carp. On the contrary, zinc (Zn) exerted an ROS scavenger and an antidote to As in the present model evidenced by alleviated liver injury and restored liver function index. Moreover, Zn and As co-administration displayed partially recovered CYPs enzyme system and quenched apoptotic positive cells compared As treated alone. These outcomes could be applied to develop counter practices based on Zn preparations to decrease the biotoxicity of As.
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Affiliation(s)
- Hongjing Zhao
- College of Wildlife and Protected Area, Northeast Forestry University, Harbin, 150040, Heilongjiang, PR China.
| | - Yu Wang
- College of Wildlife and Protected Area, Northeast Forestry University, Harbin, 150040, Heilongjiang, PR China.
| | - Menghao Guo
- College of Wildlife and Protected Area, Northeast Forestry University, Harbin, 150040, Heilongjiang, PR China
| | - Dongxue Fei
- College of Wildlife and Protected Area, Northeast Forestry University, Harbin, 150040, Heilongjiang, PR China
| | - Mengyao Mu
- College of Wildlife and Protected Area, Northeast Forestry University, Harbin, 150040, Heilongjiang, PR China
| | - Hongxian Yu
- College of Wildlife and Protected Area, Northeast Forestry University, Harbin, 150040, Heilongjiang, PR China.
| | - Mingwei Xing
- College of Wildlife and Protected Area, Northeast Forestry University, Harbin, 150040, Heilongjiang, PR China.
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Zamani N, Hassanian-Moghaddam H, Bahrami-Motlagh H, Ahmadi S, Phillips S. Lead poisoning due to ingestion of lead-contaminated opium: A diagnostic study on patients' imaging findings. J Trace Elem Med Biol 2019; 55:26-32. [PMID: 31345361 DOI: 10.1016/j.jtemb.2019.04.016] [Citation(s) in RCA: 9] [Impact Index Per Article: 1.5] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 02/27/2019] [Revised: 04/18/2019] [Accepted: 04/24/2019] [Indexed: 11/18/2022]
Abstract
OBJECTIVES Our study attempts to determine if for patients following ingestion of lead-contaminated opium, radiographs [plain X-ray (KUB)] or unenhanced computed tomography (CT scan) of the abdomen may be predictive of lead poisoning. METHODS Our study is concerned with patients of >21 years with elevated lead concentrations, who had undergone KUB or CT. Patients with other toxicities who had undergone similar imaging profiles but who had low blood lead level (BLL) were enrolled as controls. RESULTS We evaluated a total of 79 cases and 79 controls with median [IQR] BLLs of 126 [97.4, 160] μg/dL and 8.7 [5.5, 15] μg/dL. All cases and eleven controls (13.9%) were addicted to oral opium, and of these cases, anemia (94.9%) and abdominal pain (92.4%) were the two most common clinical manifestations. Two radiologists reviewed the X-ray and non-contrast CTs. Fifty (63.3%) and 53 (67.1%) cases and controls underwent CT scanning with 34 (68%) vs. 6 (11.3%) positive CTs (P < 0.001) while 43 (54.4%) and 39 (43.3%) underwent X-rays with 21 (48.8%) vs. 4 (11.8%) positive X-rays, respectively (P < 0.001). Positive CT is associated with BLL between 10 and 45 μg/dL with a specificity of 96.9%, 88.7% and positive predictive value of 97.5% and 85% respectively. CONCLUSIONS In suspected cases of lead exposure due to ingested opium, and if BLL is not readily available, a positive imaging result may guide radiologists and physicians to consider lead poisoning.
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Affiliation(s)
- Nasim Zamani
- Social Determinants of Health Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran; Department of Clinical Toxicology, Loghman-Hakim Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran
| | - Hossein Hassanian-Moghaddam
- Social Determinants of Health Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran; Department of Clinical Toxicology, Loghman-Hakim Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
| | - Hooman Bahrami-Motlagh
- Department of Radiology, Loghman-Hakim Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran
| | - Sara Ahmadi
- Department of Radiology, Loghman-Hakim Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran
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Li X, Zhang B, Li N, Ji X, Liu K, Jin M. Zebrafish neurobehavioral phenomics applied as the behavioral warning methods for fingerprinting endocrine disrupting effect by lead exposure at environmentally relevant level. CHEMOSPHERE 2019; 231:315-325. [PMID: 31132538 DOI: 10.1016/j.chemosphere.2019.05.146] [Citation(s) in RCA: 20] [Impact Index Per Article: 3.3] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 04/05/2019] [Revised: 05/16/2019] [Accepted: 05/17/2019] [Indexed: 06/09/2023]
Abstract
Environmental lead (Pb) exposure is a great hazard to the public health. Although environmentally relevant Pb poisoning is preventable, insidious Pb contaminants are still a major threat to human health. Herein, we reported that exposure to Pb at environmentally relevant concentration level (1 μg/L, 10 μg/L and 100 μg/L), disturbed the courtship behavior of adult male zebrafish and further altered the transcriptional patterns of key genes involved in testicular steroidogenesis (igf3, amh, piwil1, lhcgr, fshr, cyp11c1, star, cyp19a1a, cyp19a1b) and apoptosis (bax, cytoC, caspase 9, caspase 3, puma). Both the behavioral and the transcriptional profiles share a similar biphasic dose response, with stimulatory effects after low-level exposure and inhibitory effects after high-level exposure. This results revealed the endocrine disrupting effects of Pb even at an environmentally relevant level within the concentration range of ambient water quality criteria (AWQC) and the reliability of locomotion fingerprint as the indicator for detecting the risk induced by Pb pollution. Current research, for the first time, employed the ZebraTower system as the biological early warning system (BEWS) to find that Pb exerted biphasic effects on the courtship behavior and endocrine regulation of male adult zebrafish. Methodologically, we firstly propose an efficient solution to monitor and assess the risk of Pb exposure by combining the (BEWS) and data analyzing methods such as zebrafish phenomics, which would make a contribution to the detection and prevention of environmentally relevant Pb poisoning.
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Affiliation(s)
- Xiang Li
- Department of Medicinal Chemistry, Key Laboratory of Chemical Biology of Natural Products (Ministry of Education), School of Pharmaceutical Sciences, Shandong University, No.44 West Culture Road, Ji'nan 250012, Shandong Province, PR China
| | - Baoyue Zhang
- Biology Institute, Qilu University of Technology (Shandong Academy of Sciences), 28789 East Jingshi Road, Ji'nan, 250103, Shandong Province, PR China; Key Laboratory for Drug Screening Technology of Shandong Academy of Sciences, 28789 East Jingshi Road, Ji'nan, 250103, Shandong Province, PR China
| | - Ning Li
- Biology Institute, Qilu University of Technology (Shandong Academy of Sciences), 28789 East Jingshi Road, Ji'nan, 250103, Shandong Province, PR China; Key Laboratory for Drug Screening Technology of Shandong Academy of Sciences, 28789 East Jingshi Road, Ji'nan, 250103, Shandong Province, PR China
| | - Xiuna Ji
- Biology Institute, Qilu University of Technology (Shandong Academy of Sciences), 28789 East Jingshi Road, Ji'nan, 250103, Shandong Province, PR China; Key Laboratory for Drug Screening Technology of Shandong Academy of Sciences, 28789 East Jingshi Road, Ji'nan, 250103, Shandong Province, PR China
| | - Kechun Liu
- Biology Institute, Qilu University of Technology (Shandong Academy of Sciences), 28789 East Jingshi Road, Ji'nan, 250103, Shandong Province, PR China; Key Laboratory for Drug Screening Technology of Shandong Academy of Sciences, 28789 East Jingshi Road, Ji'nan, 250103, Shandong Province, PR China.
| | - Meng Jin
- Biology Institute, Qilu University of Technology (Shandong Academy of Sciences), 28789 East Jingshi Road, Ji'nan, 250103, Shandong Province, PR China; Key Laboratory for Drug Screening Technology of Shandong Academy of Sciences, 28789 East Jingshi Road, Ji'nan, 250103, Shandong Province, PR China.
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Selective removal of lead (II) ions and estimation of Ca (II) ions using poly-o-toluidine–zirconium (IV) molybdophosphate. CHEMICAL PAPERS 2019. [DOI: 10.1007/s11696-019-00763-4] [Citation(s) in RCA: 2] [Impact Index Per Article: 0.3] [Reference Citation Analysis] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 10/27/2022]
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