Observational Study
Copyright ©The Author(s) 2016. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Nephrol. Sep 6, 2016; 5(5): 461-470
Published online Sep 6, 2016. doi: 10.5527/wjn.v5.i5.461
Immunofluorescence on paraffin embedded renal biopsies: Experience of a tertiary care center with review of literature
Geetika Singh, Lavleen Singh, Ranajoy Ghosh, Devajit Nath, Amit Kumar Dinda
Geetika Singh, Lavleen Singh, Ranajoy Ghosh, Devajit Nath, Amit Kumar Dinda, Department of Pathology, All India Institute of Medical Sciences, New Delhi 110029, India
Author contributions: Singh G conceptualized the study, standardized the technique, wrote and critically reviewed the manuscript; Singh L compiled data and wrote the manuscript under guidance of Dr. Geetika Singh; Ghosh R and Nath D helped in standardization of technique; Dinda AK critically reviewed and edited the manuscript.
Institutional review board statement: This study was not required to provide institutional review board statement because the material used were fresh frozen tissue.
Informed consent statement: This study was not required to provide informed consent statement because the material used were fresh frozen tissue.
Conflict-of-interest statement: None.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Correspondence to: Geetika Singh, MD, Assistant Professor of Pathology, Dr BRAIRCH, Department of Pathology, All India Institute of Medical Sciences, Room No. 1084, First Floor, Teaching Block, New Delhi 110029, India. ggmed22@gmail.com
Telephone: +91-99-71911459 Fax: +91-011-26588663
Received: January 17, 2016
Peer-review started: January 18, 2016
First decision: February 29, 2016
Revised: July 16, 2016
Accepted: August 17, 2016
Article in press: August 19, 2016
Published online: September 6, 2016
Processing time: 227 Days and 17.7 Hours
Core Tip

Core tip: Immunofluorescence on formalin fixed paraffin embedded tissue is a useful “salvage” technique for renal diagnostic pathology, in case of non-availability of representative fresh frozen tissue. This article describes the technique of immunofluorescence on paraffin embedded tissue sections, discusses the potential pitfalls with an in depth review of literature.