Infectious laryngotracheitis virus in chickens

Table 1 Viral isolation from swabs in specific-pathogen-free embryonating eggs after sanitizer treatments

Sanitizer	Sample	1st d	7th d	14th d	21st d
Sodium hypochlorite	Trachea	-1	-	+2	+
	Drinker	-	-	+	+
	Biofilm	ND	ND	ND	+
Citric acid	Trachea	-	-	+	+
	Drinker	-	+	+	+
	Biofilm	ND	ND	ND	+
Sodium hydrogen sulfate	Trachea	-	-	-	-
	Drinker	-	-	-	-
	Biofilm	-	-	-	-
Hydrogen peroxide	Trachea	-	-	-	-
	Drinker	-	-	-	-
	Biofilm	-	-	-	-
Positive control3	Trachea	-	-	+	+
	Drinker	-	+	+	+
	Biofilm	+	+	+	+
Negative control4	Trachea	-	-	-	-
	Drinker	-	-	-	-
	Biofilm	-	-	-	-

¹Negative for virus isolation;

²Positive for virus isolation;

³Water lines received vaccine, but no sanitizer;

⁴Water lines received no vaccine or sanitizer. ND: Not done.

Table 2 Infectious laryngotracheitis virus isolation from beetles in specific-pathogen-free embryos

	Days after outbreak	Before H2O2 treated	After H2O2 treated
Farm 1	17	-1	-
	53	-	-
	103	-	-
Farm 2	13	2	+
	42	+	-
	90	-	-

Farm 1: Negative for virus isolation; Farm 2: Positive for virus isolation.