Review
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World J Virol. Oct 12, 2012; 1(5): 142-149
Published online Oct 12, 2012. doi: 10.5501/wjv.v1.i5.142
Infectious laryngotracheitis virus in chickens
Shan-Chia Ou, Joseph J Giambrone
Shan-Chia Ou, Joseph J Giambrone, Department of Poultry Science, Auburn University, Auburn, AL 36849, United States
Author contributions: Ou SC did the research and Giambrone J J wrote the paper.
Correspondence to: Joseph J Giambrone, Professor, Department of Poultry Science, Auburn University, Auburn, AL 36849, United States. giambjj@auburn.edu
Telephone: +1-334-8442642 Fax: +1-334-8442641
Received: August 8, 2011
Revised: June 12, 2012
Accepted: September 7, 2012
Published online: October 12, 2012
Abstract

Infectious laryngotracheitis (ILT) is an important respiratory disease of chickens and annually causes significant economic losses in the poultry industry world-wide. ILT virus (ILTV) belongs to alphaherpesvirinae and the Gallid herpesvirus 1 species. The transmission of ILTV is via respiratory and ocular routes. Clinical and post-mortem signs of ILT can be separated into two forms according to its virulence. The characteristic of the severe form is bloody mucus in the trachea with high mortality. The mild form causes nasal discharge, conjunctivitis, and reduced weight gain and egg production. Conventional polymerase chain reaction (PCR), nested PCR, real-time PCR, and loop-mediated isothermal amplification were developed to detect ILTV samples from natural or experimentally infected birds. The PCR combined with restriction fragment length polymorphism (RFLP) can separate ILTVs into several genetic groups. These groups can separate vaccine from wild type field viruses. Vaccination is a common method to prevent ILT. However, field isolates and vaccine viruses can establish latent infected carriers. According to PCR-RFLP results, virulent field ILTVs can be derived from modified-live vaccines. Therefore, modified-live vaccine reversion provides a source for ILT outbreaks on chicken farms. Two recently licensed commercial recombinant ILT vaccines are also in use. Other recombinant and gene-deficient vaccine candidates are in the developmental stages. They offer additional hope for the control of this disease. However, in ILT endemic regions, improved biosecurity and management practices are critical for improved ILT control.

Keywords: Infectious laryngotracheitis virus; Infectious laryngotracheitis; Gallid herpesvirus-1; Polymerase chain reaction combined with restriction fragment length polymorphism; Recombinant laryngotracheitis vaccines