Development of a multiplex polymerase chain reaction assay for detection of hepatitis C virus, hepatitis B virus, and human immunodeficiency virus 1

doi:10.5501/wjv.v13.i1.88164

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World Journal of Virology

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2220-3249

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Virol. Mar 25, 2024; 13(1): 88164
Published online Mar 25, 2024. doi: 10.5501/wjv.v13.i1.88164

Development of a multiplex polymerase chain reaction assay for detection of hepatitis C virus, hepatitis B virus, and human immunodeficiency virus 1

Waleed Abdelgaber Nemr, Radwan K Nashwa

Waleed Abdelgaber Nemr, Department of Radiation Microbiology, National Center for Radiation Research and Technology, Egyptian Atomic Energy Authority, Cairo 11371, Egypt

Radwan K Nashwa, Department of Health Radiation Research, National Center for Radiation Research and Technology, Egyptian Atomic Energy Authority, Cairo 11371, Egypt

Author contributions: Both of the listed authors contributed to the present work equally; both authors read and approved the final manuscript.

Institutional review board statement: The current study has not subjected human or animals to any experiment.

Conflict-of-interest statement: All the authors report no relevant conflicts of interest for this article.

Data sharing statement: No additional data are available.

Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/

Corresponding author: Waleed Abdelgaber Nemr, PhD, Doctor, Department of Radiation Microbiology, National Center for Radiation Research and Technology, Egyptian Atomic Energy Authority, 3^th Ahmed Alzomor Street, Cairo 11371, Egypt. wnemr@hotmail.com

Received: September 16, 2023
Peer-review started: September 16, 2023
First decision: November 9, 2023
Revised: December 7, 2023
Accepted: January 10, 2024
Article in press: January 10, 2024
Published online: March 25, 2024
Processing time: 177 Days and 1.1 Hours

Core Tip

Core Tip: The current study approaches a cost-effective diagnostic assay to detect the most common blood-borne viruses (hepatitis C virus, hepatitis B virus, and human immunodeficiency virus 1) in a single test of multiplex polymerase chain reaction (PCR). This article includes the procedures in computational biology to achieve the PCR design and the practical examination of the given assay for detecting the targeted viruses with the interpretation of the results.