Effectiveness of repeated transplantations of hematopoietic stem cells in spinal cord injury

Table 1 Sex, age, level of injury distribution of patients with traumatic disease of spinal cord (main group)

No. of patients	202 (1008 case histories)
Age	From 19 to 51 yr
Gender	Males - 156, females - 46
Years post injury	Less than 1 yr - 11
	From 1 to 5 yr - 144
	Over 5 yr - 47
Level of spinal cord injury	Cervical level - 93
	Thoracic level - 98
	Lumbar level - 11
Type of injury	Complete - 43
	Incomplete - 159
No. of transplantations	No less than 20 HSCs and HPs transplantations
Average number of transplanted cells	5.8 × 106

HSCs: Hematopoietic stem cells; HPs: Hematopoietic precursors.

Table 2 Sex, age, level of injury distribution of patients with traumatic disease of spinal cord (control group)

No. of patients	20 (62 case histories)
Age	From 18 to 44 yr
Gender	Males - 13, females - 7
Years post injury	< 1 yr - 6
	From 1 to 5 yr - 10
	Over 5 yr - 4
Level of spinal cord injury	Cervical level - 14
	Thoracic level - 4
	Lumbar level - 2
Type	Complete - 12
	Incomplete - 8

Table 3 The characteristics and basic differences of the hematopoietic stem cells and hematopoietic precursors preparation from the preparation of hematopoietic stem cells used for bone marrow transplantation

Technique	G-CSF dose	Period of administration (d)	Stimulation regimen	Cell markers	Cryopreservant
Administration of HSCs in blood	10-20 μg/kg	6-7	1 in 24 h	CD34+, CD45+	10%-20% DMSO
				HLA DR+, CD38+
				Gp130±
Administration of HSC and HPs in CSF	5 μg/kg; double dose at day 4	5	2 in 24 h	CD34+, CD45-	5%-10% DMSO +
				HLA DR±, CD38±	polyglucin
				Gp130+

HSCs: Hematopoietic stem cells; HPs: Hematopoietic precursors; G-CSF: Granulocyte-colony stimulating factor.

Table 4 Clinical symptoms of the complications and side effects in spinal cord injury patients

Symptoms	Stages of research			Control group patients
	1 stage	2 stage	3 stage
Increased spasticity	46%	49.9%	54.5%	0
Fever	15%	19%	18.8%	0
Post-puncture headache	11%	14.9%	12.2%	14.9%
High blood pressure	10%	8.1%	14.5%	0
Coordination disturbance	2.3%	1.3%	0	0
Dizziness	2%	3.4%	0	4.2%
Sleepiness	2.1%	1.7%	0	0
Emotional lability	1.6%	1.7%	0	0
Disordered consciousness	1.2%	0.8%	0.8%	0
Meningism	3.7%	2.95%	0.8%	0
Low blood pressure	1.68%	2.95%	5.9%	0
General % of the patients with complications	63.5%	72.9%	75%	19.1%