Scalera I, Franzin R, Stasi A, Castellaneta A, Fischetti E, Morelli G, Raele M, Panetta E, Kurevija A, Pulga W, Atti M, Gesualdo L. Haemoadsorption cartridge connected to the machine perfusion for donation after circulatory death porcine liver marginal grafts. World J Transplant 2025; 15(2): 99287 [DOI: 10.5500/wjt.v15.i2.99287]
Corresponding Author of This Article
Irene Scalera, MD, PhD, Doctor, Surgeon, Hepatobiliary and Liver Transplant Unit, University Hospital Policlinic of Bari, Piazza G Cesare 11, Bari 70124, Puglia, Italy. irenescalera@gmail.com
Research Domain of This Article
Transplantation
Article-Type of This Article
Basic Study
Open-Access Policy of This Article
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Irene Scalera, Enrico Fischetti, Giulia Morelli, Margherita Raele, Hepatobiliary and Liver Transplant Unit, University Hospital Policlinic of Bari, Bari 70124, Puglia, Italy
Rossana Franzin, Alessandra Stasi, Loreto Gesualdo, Division of Nephrology, Department of Dialysis and Transplant, University Hospital Policlinic of Bari, Bari 70124, Puglia, Italy
Antonino Castellaneta, Gastroenterology and Digestive Endoscopy Unit, University Hospital Policlinic of Bari, Bari 70124, Puglia, Italy
Emilio Panetta, Andjela Kurevija, William Pulga, Mauro Atti, Department of Aferetica, Aferetica Srl, Bologna 40138, Emilia-Romagna, Italy
Author contributions: Scalera I designed the research study and wrote the manuscript; Fischetti E, Morelli G, and Raele M did the surgical part of the research; Stasi A and Frazin R did the research, analyzed the specimens and the data and wrote the manuscript; Castellaneta A and Gesualdo L reviewed the manuscript; Panetta E, Kureveija A, Pulga W and Atti M collaborated for the logistics; all authors read and approved the final version of the manuscript to be published.
Institutional review board statement: As the study used organs after the slaughter, it didn't require any particular board approbation.
Institutional animal care and use committee statement: All necessary standard measures were taken to preserve the animal welfare, as required by European legislation (EC 1099/2009).
Conflict-of-interest statement: All authors declare no conflict of interest in publishing the manuscript.
ARRIVE guidelines statement: The authors have read the ARRIVE guidelines, and the manuscript was prepared and revised according to the ARRIVE guidelines.
Data sharing statement: Technical aspect for retrieving the grafts after the slaughter are available from the corresponding author at irenescalera@gmail.com.
Open Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Irene Scalera, MD, PhD, Doctor, Surgeon, Hepatobiliary and Liver Transplant Unit, University Hospital Policlinic of Bari, Piazza G Cesare 11, Bari 70124, Puglia, Italy. irenescalera@gmail.com
Received: July 18, 2024 Revised: November 7, 2024 Accepted: January 11, 2025 Published online: June 18, 2025 Processing time: 217 Days and 13.3 Hours
Core Tip
Core Tip: Marginal donation after circulatory death liver grafts are carefully used to fight the constant shortage of donors. Clinically, the worst outcomes are mainly related to severe ischemia-reperfusion-injury and the dangerous effect of various inflammatory cytokines (CK). The machine perfusion (MP) is a promising device to rescue these grafts. Recently, a new ex-situ MP device incorporating a filter cartridge to the machine has been considered. It was tested on pigs, and it appears to reduce the CK during the treatment of standard liver grafts. The purpose of the study was the analysis of the role of the CK cartridge connected to the MP and used to preserve extremely marginal pig grafts during processing in the MP. The results were analyzed biochemically and histologically.
