Takata M, Tanaka Y, Saito D, Yoshida S, Matsumoto I. Hyperacute experimental model of rat lung transplantation using a coronary shunt cannula. World J Transplant 2024; 14(2): 92137 [DOI: 10.5500/wjt.v14.i2.92137]
Corresponding Author of This Article
Isao Matsumoto, MD, PhD, Professor, Department of Thoracic Surgery, Kanazawa University, 13-1 Takara-machi, Kanazawa 920-8641, Ishikawa, Japan. isa-mat@med.kanazawa-u.ac.jp
Research Domain of This Article
Transplantation
Article-Type of This Article
Basic Study
Open-Access Policy of This Article
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Munehisa Takata, Yusuke Tanaka, Daisuke Saito, Shuhei Yoshida, Isao Matsumoto, Department of Thoracic Surgery, Kanazawa University, Kanazawa 920-8641, Ishikawa, Japan
Co-first authors: Munehisa Takata and Yusuke Tanaka.
Author contributions: Takata M and Tanaka Y contributed equally to this work in every aspect of the study design, performed experiments, data collection and analysis, and manuscript preparation; Matsumoto I contributed to the study design and writing; all authors interpreted the data and approved the final version of the article.
Institutional animal care and use committee statement: Experimental animals were used following the Guidelines for the Care and Use of Laboratory Animals of Kanazawa University (approval no.: AP-163743) and the Guide for the Care and Use of Laboratory Animals (8th edition) published by the National Research Council in 2011.
Conflict-of-interest statement: All the authors have no conflicts of interest to declare.
Data sharing statement: No additional data are available.
ARRIVE guidelines statement: The authors have read the ARRIVE guidelines, and the manuscript was prepared and revised according to the ARRIVE guidelines.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Isao Matsumoto, MD, PhD, Professor, Department of Thoracic Surgery, Kanazawa University, 13-1 Takara-machi, Kanazawa 920-8641, Ishikawa, Japan. isa-mat@med.kanazawa-u.ac.jp
Received: January 16, 2024 Revised: February 21, 2024 Accepted: April 8, 2024 Published online: June 18, 2024 Processing time: 149 Days and 22.4 Hours
Abstract
BACKGROUND
Lung transplantation is a well-established treatment of end-stage lung disease. A rodent model is an inexpensive way to collect biological data from a living model after lung transplantation. However, mastering the surgical technique takes time owing to the small organ size.
AIM
To conduct rat lung transplantation using a shunt cannula (SC) or modified cannula (MC) and assess their efficacy.
METHODS
Rat lung transplantation was performed in 11 animals in the SC group and 12 in the MC group. We devised a method of rat lung transplantation using a coronary SC for coronary artery bypass surgery as an anastomosis of pulmonary arteriovenous vessels and bronchioles. The same surgeon performed all surgical procedures in the donor and recipient rats without using a magnifying glass. The success rate of lung transplantation, operating time, and PaO2 values were compared after 2-h reperfusion after transplantation.
RESULTS
Ten and 12 lungs were successfully transplanted in the SC and MC groups, respectively. In the SC group, one animal had cardiac arrest within 1 h after reperfusion owing to bleeding during pulmonary vein anastomosis. The operating time for the removal of the heart-lung block from the donor and preparation of the left lung graft was 26.8 ± 2.3 and 25.7 ± 1.3 min in the SC and MC groups, respectively (P = 0.21). The time required for left lung transplantation in the recipients was 37.5 ± 2.8 min and 35.9 ± 1.4 min in the SC and MC groups, respectively (P = 0.12). PaO2 values at 2 h after reperfusion were 456.2 ± 25.5 and 461.2 ± 21.5 mmHg in the SC and MC groups, respectively (P = 0.63), without difference between the groups.
CONCLUSION
A hyperacute rat lung transplantation model using a coronary SC was created using a simple technique. The MC was inexpensive, easy to prepare, and simple to operate.
Core Tip: We developed a rat lung transplantation technique using a coronary shunt cannula (SC) for the pulmonary arteriovenous system and bronchial tube anastomosis. This method is simple and can be performed by a surgeon. This study evaluated the usefulness of this method by using a modified cannula (MC), which we developed by modifying the SC and improving its shortcomings. The MC is inexpensive and easy to prepare and operate. Presently, a hyperacute lung transplantation model is feasible, and future improvements, such as a smaller cannula and reduced foreign body reaction, will be made to create a chronic rat lung transplantation model.
