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World J Transplant. Nov 28, 2020; 10(11): 330-344
Published online Nov 28, 2020. doi: 10.5500/wjt.v10.i11.330
Extracellular vesicles as mediators of alloimmunity and their therapeutic potential in liver transplantation
Sotiris Mastoridis, Marc Martinez-Llordella, Alberto Sanchez-Fueyo
Sotiris Mastoridis, Department ofSurgery, Oxford University Hospitals, Oxford OX37LE, United Kingdom
Marc Martinez-Llordella, Institute of Liver Studies, King's College Hospital, Medical Research Council (MRC) Centre for Transplantation, London SE59NU, United Kingdom
Alberto Sanchez-Fueyo, Department of Liver Sciences, King's College Hospital, Medical Research Council (MRC) Centre for Transplantation, London SE59NU, United Kingdom
Author contributions: Mastoridis S wrote the manuscript with support and consultation from Martinez-Llordella M and Sanchez-Fueyo A; all authors approve the final manuscript.
Conflict-of-interest statement: There are no conflicts of interest to be reported.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Sotiris Mastoridis, MBBS, PhD, Doctor, Department of Surgery, Oxford University Hospitals, Churchill Hospital, Old Road, Oxford, Oxford OX37LE, United Kingdom. sotiris.mastoridis@googlemail.com
Received: August 31, 2020
Peer-review started: August 31, 2020
First decision: October 23, 2020
Revised: November 6, 2020
Accepted: November 17, 2020
Article in press: November 17, 2020
Published online: November 28, 2020
Processing time: 82 Days and 2.8 Hours
Abstract

Extracellular vesicles (EVs) are a heterogenous group of nanosized, membrane-bound particles which are released by most cell types. They are known to play an essential role in cellular communication by way of their varied cargo which includes selectively enriched proteins, lipids, and nucleic acids. In the last two decades, wide-ranging evidence has established the involvement of EVs in the regulation of immunity, with EVs released by immune and non-immune cells shown to be capable of mediating immune stimulation or suppression and to drive inflammatory, autoimmune, and infectious disease pathology. More recently, studies have demonstrated the involvement of allograft-derived EVs in alloimmune responses following transplantation, with EVs shown to be capable of eliciting allograft rejection as well as promoting tolerance. These insights are necessitating the reassessment of standard paradigms of T cell alloimmunity. In this article, we explore the latest understanding of the impact of EVs on alloresponses following transplantation and we highlight the recent technological advances which have enabled the study of EVs in clinical transplantation. Furthermore, we discuss the rapid progress afoot in the development of EVs as novel therapeutic vehicles in clinical transplantation with particular focus on liver transplantation.

Keywords: Extracellular vesicle; Transplantation; Liver; Alloimmunity; Tolerance; Therapy

Core Tip: Extracellular vesicles (EVs) are key contributors to T cell alloimmunity through the transfer of major histocompatibility alloantigens to host antigen presenting cells (APCs) thereby initiating alloresponses and acute rejection. Strong circumstantial evidence suggests that under certain conditions EV-mediated cross-dressing of recipient APCs can also tolerance responses and allay allograft rejection–for instance in the context of liver transplantation. We anticipate improved mechanistic understanding of these processes will facilitate design of novel EV therapies in transplantation. A number of clinical trials assessing the safety and efficacy of EVs are underway. The substantial developments in engineered Good Manufacture Practices-grade EVs hold promise for novel EV-therapeutics in transplantation and beyond.