Basic Study
Copyright ©The Author(s) 2025.
World J Exp Med. Jun 20, 2025; 15(2): 100443
Published online Jun 20, 2025. doi: 10.5493/wjem.v15.i2.100443
Table 1 Short tandem repeat analysis
Microsatellite (chromosome number)
OCUG-2 cells
D3S1358 (3)16
TH01 (11)7, 9
D21S11 (21)30
D18S51 (18)13
Penta E (15)12
D5S818 (5)12
D13S317 (13)12
D7S820 (7)9, 12
D16S539 (16)9, 10
CSF1PO (5)12
Penta D (21)11
Amelogenin (X Y)X
vWA (12)16
D8S1179 (8)13, 15
TPOX (2)8, 12
FGA (4)20, 21
Table 2 Correlation between clinicopathologic features and insulin-like growth factor 1 receptor expression in gallbladder cancer
Feature
IGF1R-positive (n = 17)
IGF1R-negative (n = 17)
P value
Sex
    Male610
    Female1170.17
Chemotherapy
    Yes1110
    No670.72
T stage
    T1/T2810
    T3/T4970.49
N stage
    Negative812
    Positive950.16
M stage
    Negative1416
    Positive310.28
Clinical stage
    I/II610
    III/IV1170.16
Table 3 Univariate and multivariate regression analyses of overall survival
FeatureUnivariate analysis
Multivariate analysis
Hazard ratio (95%CI)
P value
Hazard ratio (95%CI)
P value
Sex0.658 (0.259-1.672)NS
Chemotherapy3.039 (1.077-8.578)0.031.969 (0.594-6.517)NS
T1.771 (1.165-2.692)0.005
N2.833 (1.2-6.687)0.01
M4.905 (1.516-15.88)0.003
Stage1.767 (1.146-2.723)0.0061.595 (0.950-2.679)0.077
IGF1R expression2.654 (1.102-6.913)0.043.302 (1.208-9.023)0.02
Table 4 Candidate gene fusions detected in RNA sequencing data of OCUG-2 cells
Gene fusion
Breakpoint1
Breakpoint2
Confidence
Reading frame
LEPROT: LEPRchr1:65425378chr1:65565546HighOut-of-frame
LEPROT: LEPRchr1:65430048chr1:65565546MediumOut-of-frame
TSPAN14: AL392185.1chr10:80504778chr9:95602159HighOut-of-frame
TSPAN14: AL392185.1chr10:80489314chr9:95602159HighOut-of-frame
TSPAN14: AL392185.1chr10:80506862chr9:95602874Medium
TSPAN14: AL392185.1chr10:80504778chr9:95600165MediumOut-of-frame
RNF115: ITGA10chr1:145823772chr1:145907465HighOut-of-frame
PCDHA3: PCDHAC2chr5:140803591chr5:140978949HighIn-frame
AC092807.3: DDAH1chr1:85496166chr1:85358847High
AC092807.3: DDAH1chr1:85494729chr1:85358847Medium
AC092807.3: DDAH1chr1:85577984chr1:85358847Medium
ERC1: PDE3Achr12:1141787chr12:20556660HighIn-frame
TVP23C: CDRT4chr17:15540433chr17:15440285HighOut-of-frame
PRR16: AC114284.1chr5:120464645chr5:120789954HighOut-of-frame
PCDHGB2: PCDHGA8chr5:141362556chr5:141494807MediumIn-frame
MYO9A: PKMchr15:72117680chr15:72219110Medium
RASSF8: SSPNchr12:26055446chr12:26224293MediumOut-of-frame
RASSF8: SSPNchr12:26055446chr12:26179996MediumOut-of-frame
AC104123.1: CASTchr5:96379652chr5:96675539Medium
MGST1: AC007529.2chr12:16383604chr12:16661766Medium
LDLRAD3: PRR5Lchr11:36098461chr11:36296290MediumOut-of-frame