Basic Study
Copyright ©The Author(s) 2025. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Exp Med. Jun 20, 2025; 15(2): 100443
Published online Jun 20, 2025. doi: 10.5493/wjem.v15.i2.100443
Establishment and characterization of a new human gallbladder cancer cell line, OCUG-2
Qiang Wang, Canfeng Fan, Gen Tsujio, Takashi Sakuma, Koji Maruo, Yurie Yamamoto, Daiki Imanishi, Kyoka Kawabata, Hinano Nishikubo, Saki Kanei, Rika Aoyama, Syuhei Kushiyama, Masaichi Ohira, Masakazu Yashiro
Qiang Wang, Canfeng Fan, Gen Tsujio, Takashi Sakuma, Koji Maruo, Yurie Yamamoto, Daiki Imanishi, Kyoka Kawabata, Hinano Nishikubo, Saki Kanei, Rika Aoyama, Syuhei Kushiyama, Masaichi Ohira, Masakazu Yashiro, Department of Molecular Oncology and Therapeutics, Osaka Metropolitan University Graduate School of Medicine, Osaka 545-0051, Japan
Qiang Wang, Canfeng Fan, Gen Tsujio, Takashi Sakuma, Koji Maruo, Yurie Yamamoto, Kyoka Kawabata, Hinano Nishikubo, Saki Kanei, Rika Aoyama, Syuhei Kushiyama, Masaichi Ohira, Masakazu Yashiro, Cancer Center for Translational Research, Osaka Metropolitan University Graduate School of Medicine, Osaka 545-0051, Japan
Gen Tsujio, Takashi Sakuma, Koji Maruo, Daiki Imanishi, Syuhei Kushiyama, Masaichi Ohira, Masakazu Yashiro, Department of Gastroenterological Surgery, Osaka Metropolitan University Graduate School of Medicine, Osaka 545-0051, Japan
Author contributions: Wang Q and Yashiro M designed and performed the experiments; Fan C, Tsujio G, Sakuma T, Maruo K, and Yamamoto Y performed the sample preparation; Imanishi D, Kawabata K, Nishikubo H, Kanei S, Aoyama R, and Kushiyama S conducted the material sampling; Ohira M performed the data collection; Yashiro M performed the manuscript review. All authors read and approved the final manuscript.
Institutional review board statement: The patient provided written informed consent in accordance with our IRB-approved protocol by Osaka Metropolitan University Graduate School of Medicine (approval number # 0924 and # 4391).
Institutional animal care and use committee statement: The principles of laboratory animal care and animal use were observed for the present study with the approval of the Ethical Committee for Animal Research of Osaka Metropolitan University Graduate School of Medicine (Protocol # 19001).
Conflict-of-interest statement: The authors declare that there is no conflict of interest regarding the publication of this paper.
ARRIVE guidelines statement: The authors have read the ARRIVE guidelines, and the manuscript was prepared and revised according to the ARRIVE guidelines.
Data sharing statement: sharing statement: All relevant data are within the manuscript and its Supporting Information file.
Open Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Masakazu Yashiro, MD, PhD, Associate Professor, Department of Molecular Oncology and Therapeutics, Osaka Metropolitan University Graduate School of Medicine, 1-4-3 Asahi-machi, Abeno-ku, Osaka 545-0051, Japan. i21496f@omu.ac.jp
Received: August 20, 2024
Revised: November 19, 2024
Accepted: January 7, 2025
Published online: June 20, 2025
Processing time: 242 Days and 18.9 Hours
Core Tip

Core Tip: We established a new gallbladder cancer (GBC) cell line derived from a metastatic peritoneal implant of GBC. These cells, named OCUG-2, showed adhesive growth with a dendritic morphology. Doubling time of OCUG-2 cells was 30 hours. OCUG-2 cells were positive for insulin-like growth factor 1 receptor (IGF1R), and an IGF1R inhibitor reduced the proliferation of OCUG-2 cells. Immunohistochemistry found IGF1R positivity in 18 of 34 cases of GBC, and IGF1R expression was associated with poor prognosis. These findings suggest that IGF1R may be a promising target for GBC and OCUG-2 might be useful for analysis of GBC.