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©The Author(s) 2015. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Exp Med. May 20, 2015; 5(2): 140-153
Published online May 20, 2015. doi: 10.5493/wjem.v5.i2.140
Published online May 20, 2015. doi: 10.5493/wjem.v5.i2.140
Barriers in contribution of human mesenchymal stem cells to murine muscle regeneration
Anabel S de la Garza-Rodea, Children’s Hospital Oakland Research Institute, Oakland, CA 94609, United States
Hester Boersma, Dirk W van Bekkum, Shoshan Knaän-Shanzer, Virus and Stem Cell Biology Laboratory, Department of Molecular Cell Biology, Leiden University Medical Center, Leiden 2333 ZC, the Netherlands
Cheryl Dambrot, Yale School of Medicine, New Haven, CT 06510, United States
Antoine AF de Vries, Department of Cardiology, Leiden University Medical Center, Leiden 2333 ZC, the Netherlands
Author contributions: de la Garza-Rodea AS, Boersma H and Dambrot C performed experiments; de la Garza-Rodea AS, Boersma H, de Vries AAF and Knaän-Shanzer S analyzed the data; de la Garza-Rodea AS, van Bekkum DW and Knaän-Shanzer S designed the study and wrote the manuscript.
Supported by A scholarship to AS de la Garza-Rodea from the Universidad Autonoma de Nuevo Leon, Monterrey, Mexico.
Ethics approval: Approval from ethical committee obtained.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Correspondence to: Dr. Shoshan Knaän-Shanzer, Virus and Stem Cell Biology Laboratory, Department of Molecular Cell Biology, Leiden University Medical Center, Einthovenweg 20, Leiden 2333 ZC, the Netherlands. shoshanknaan@gmail.com
Telephone: +31-71-5269246 Fax: +31-62-4486785
Received: August 25, 2014
Peer-review started: August 26, 2014
First decision: October 14, 2014
Revised: December 27, 2014
Accepted: February 4, 2015
Article in press: February 9, 2015
Published online: May 20, 2015
Processing time: 269 Days and 3.8 Hours
Peer-review started: August 26, 2014
First decision: October 14, 2014
Revised: December 27, 2014
Accepted: February 4, 2015
Article in press: February 9, 2015
Published online: May 20, 2015
Processing time: 269 Days and 3.8 Hours
Core Tip
Core tip: The translational relevance of animal models for tissue repair is often ambiguous. We describe here a murine model for the comparison of the regeneration of damaged human and murine skeletal muscle implants and the contribution of human and mouse mesenchymal stem cells (MSCs) to this process. Our findings suggest that murine muscle tissue provides a suboptimal environment for maintenance of human MSC, and that in mouse models their capacity to engage in myoregeneration is underestimated. The added value of the present model is that it permits the dissection of species-specific factors in the microenvironment.