Reference Citation Analysis: Find an Article, Find a Category, Find a Journal, Find a Scholar

For: Chen L, Zhao J, Peng J, Li X, Deng X, Geng Z, Shen Z, Guo F, Zhang Q, Jin Y, Wang L, Wang S. Detection of SARS-CoV-2 in saliva and characterization of oral symptoms in COVID-19 patients. Cell Prolif 2020;53:e12923. [PMID: 33073910 PMCID: PMC7645955 DOI: 10.1111/cpr.12923] [Citation(s) in RCA: 105] [Impact Index Per Article: 21.0] [Reference Citation Analysis] [What about the content of this article? (0)] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 07/31/2020] [Revised: 09/16/2020] [Accepted: 09/20/2020] [Indexed: 01/08/2023] Open

For:	Chen L, Zhao J, Peng J, Li X, Deng X, Geng Z, Shen Z, Guo F, Zhang Q, Jin Y, Wang L, Wang S. Detection of SARS-CoV-2 in saliva and characterization of oral symptoms in COVID-19 patients. Cell Prolif 2020;53:e12923. [PMID: 33073910 PMCID: PMC7645955 DOI: 10.1111/cpr.12923] [Citation(s) in RCA: 105] [Impact Index Per Article: 21.0] [Reference Citation Analysis] [What about the content of this article? (0)] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 07/31/2020] [Revised: 09/16/2020] [Accepted: 09/20/2020] [Indexed: 01/08/2023] Open

Number

Cited by Other Article(s)

Bhanu P, Buchke S, Hemandhar-Kumar N, Varsha P, Kiran SKR, Vikneswaran G, Alva A, Basavaraj GS, Kumar J. Comparative metagenomic analysis of the oral microbiome in COVID-19 patients and healthy individuals. Sci Rep 2025;15:10303. [PMID: 40133298 PMCID: PMC11937335 DOI: 10.1038/s41598-024-81864-3] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/19/2024] [Accepted: 11/29/2024] [Indexed: 03/27/2025] Open

Sette-de-Souza PH, Fernandes Costa MJ, Dutra Borges BC. SARS-CoV-2 proteins show great binding affinity to resin composite monomers and polymerized chains. World J Exp Med 2025;15:94022. [PMID: 40115751 PMCID: PMC11718582 DOI: 10.5493/wjem.v15.i1.94022] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 03/10/2024] [Revised: 10/03/2024] [Accepted: 10/30/2024] [Indexed: 12/26/2024] Open

Jimenez-Campos AG, Maestas LI, Velappan N, Beck B, Ye C, Wernsing K, Mata-Solis Y, Bruno WJ, Bussmann SC, Bradfute S, Baca JT, Rininsland FH. A cell-based Papain-like Protease (PLpro) activity assay for rapid detection of active SARS-CoV-2 infections and antivirals. PLoS One 2024;19:e0309305. [PMID: 39724215 DOI: 10.1371/journal.pone.0309305] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 06/10/2024] [Accepted: 08/09/2024] [Indexed: 12/28/2024] Open

Abstract

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and its variants are a continuous threat to human life. An urgent need remains for simple and fast tests that reliably detect active infections with SARS-CoV-2 and its variants in the early stage of infection. Here we introduce a simple and rapid activity-based diagnostic (ABDx) test that identifies SARS-CoV-2 infections by measuring the activity of a viral enzyme, Papain-Like protease (PLpro). The test system consists of a peptide that fluoresces when cleaved by SARS PLpro that is active in crude, unprocessed lysates from human tongue scrapes and saliva. Test results are obtained in 30 minutes or less using widely available fluorescence plate readers, or a battery-operated portable instrument for on-site testing. Proof-of-concept was obtained in a study on clinical specimens collected from patients with COVID-19 like symptoms who tested positive (n = 10) or negative (n = 10) with LIAT RT-PCR using nasal mid turbinate swabs. When saliva from these patients was tested with in-house endpoint RT-PCR, 17 were positive and only 5 specimens were negative, of which 2 became positive when tested 5 days later. PLpro activity correlated in 17 of these cases (3 out of 3 negatives and 14 out of 16 positives, with one invalid specimen). Despite the small number of samples, the agreement was significant (p value = 0.01). Two false negatives were detected, one from a sample with a late Ct value of 35 in diagnostic RT-PCR, indicating that an active infection was no longer present. The PLpro assay is easily scalable and expected to detect all viable SARS-CoV-2 variants, making it attractive as a screening and surveillance tool. Additionally, we show feasibility of the platform as a new homogeneous phenotypic assay for rapid screening of SARS-CoV-2 antiviral drugs and neutralizing antibodies.

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Graves C, Ghaltakhchyan N, Ngo TQ, Liu C, Babikow E, Shoji A, Bocklage C, Sang Y, Phillips ST, Bowman N, Frazier-Bowers S, Freire M, Wallet S, Divaris K, Wu D, Jacox LA. A Cetylpyridinium Chloride Oral Rinse Reduces Salivary Viral Load in Randomized Controlled Trials. JDR Clin Trans Res 2024:23800844241296840. [PMID: 39651628 DOI: 10.1177/23800844241296840] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/11/2024] Open

Abstract

INTRODUCTION

Evaluating the antiviral potential of commercially available mouthrinses on SARS-CoV-2 holds potential for reducing transmission, particularly as novel variants emerge. Because SARS-CoV-2 is transmitted primarily through salivary and respiratory secretions and aerosols, strategies to reduce salivary viral burden in an antigen-agnostic manner are attractive for mitigating spread in dental, otolaryngology, and orofacial surgery clinics where patients may need to unmask.

METHODS

Patients (n = 128) with confirmed COVID-19-positive status within 10 days of symptom onset or positive test result were enrolled in a double-blind randomized controlled trial of Food and Drug Administration-approved mouthrinses containing active ingredients ethanol, hydrogen peroxide, povidone iodine, chlorhexidine gluconate, cetylpyridinium chloride (CPC), or saline. The CPC, ethanol, and sterile water rinses were followed in a second double-blind randomized controlled trial (n = 230). Participants provided a saliva sample before rinsing (baseline) and again at 30 and 60 min after rinse. Quantitative polymerase chain reaction was used to determine salivary SARS-CoV-2 viral load at all time points. An adjusted linear mixed-effect model was employed to compare viral load after rinsing relative to baseline.

RESULTS

The rinse containing CPC significantly reduced salivary SARS-CoV-2 viral load 30 min postrinse relative to baseline (P = .015), whereas no other rinse significantly affected viral load at 30 min after rinsing. At 60 min postrinsing, no group had a significant reduction in SARS-CoV-2 copy number relative to baseline, indicating a rebound in salivary viral load over a 1-hour window. Participants indicated a fair to good rinsing experience with the CPC product and high willingness to use oral rinses before and during dental and medical health care visits.

CONCLUSIONS

Our findings suggest that preprocedural oral rinsing could be implemented as a feasible, inexpensive approach to mitigate spread of SARS-CoV-2 and potentially other enveloped viruses for short periods, which is relevant to clinical procedures involving the nasal and oropharyngeal region.

KNOWLEDGE TRANSFER STATEMENT

Rinsing with a cetylpyridinium chloride-containing mouthrinse can significantly reduce salivary SARS-CoV-2 viral load for up to 30 min; patients are willing to use mouthrinses in medical and dental settings to limit transmission risk in clinics.

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Affiliation(s)

C Graves Department of Biomedical Sciences, Adams School of Dentistry, University of North Carolina, Chapel Hill, NC, USA
N Ghaltakhchyan Department of Biomedical Sciences, Adams School of Dentistry, University of North Carolina, Chapel Hill, NC, USA Department of Orthodontics, Adams School of Dentistry, University of North Carolina, Chapel Hill, NC, USA
T Q Ngo Department of Biomedical Sciences, Adams School of Dentistry, University of North Carolina, Chapel Hill, NC, USA Department of Orthodontics, Adams School of Dentistry, University of North Carolina, Chapel Hill, NC, USA
C Liu Department of Biostatistics, University of North Carolina, Chapel Hill, NC, USA
E Babikow Department of Orthodontics, Adams School of Dentistry, University of North Carolina, Chapel Hill, NC, USA
A Shoji Department of Biomedical Sciences, Adams School of Dentistry, University of North Carolina, Chapel Hill, NC, USA Department of Orthodontics, Adams School of Dentistry, University of North Carolina, Chapel Hill, NC, USA
C Bocklage Department of Orthodontics, Adams School of Dentistry, University of North Carolina, Chapel Hill, NC, USA
Y Sang Department of Biomedical Sciences, Adams School of Dentistry, University of North Carolina, Chapel Hill, NC, USA
S T Phillips Department of Biostatistics, University of North Carolina, Chapel Hill, NC, USA
N Bowman School of Medicine, University of North Carolina, Chapel Hill, NC, USA
S Frazier-Bowers Department of Orthodontics, Adams School of Dentistry, University of North Carolina, Chapel Hill, NC, USA
M Freire J. Craig Venter Institute, La Jolla, CA, USA
S Wallet Department of Oral Biology, College of Dentistry, University of Florida, Gainesville, FL, USA
K Divaris Department of Pediatric Dentistry and Dental Public Health, Adams School of Dentistry, University of North Carolina, Chapel Hill, NC, USA Department of Epidemiology, Gillings School of Global Public Health, University of North Carolina, Chapel Hill, CA, USA. Present address for E.B.: Parrott Orthodontics, Staunton, VA, USA. Present address for S.F.B.: School of Dentistry, Indiana University, Indianapolis, Indiana, USA
D Wu Department of Biostatistics, University of North Carolina, Chapel Hill, NC, USA
L A Jacox Department of Biomedical Sciences, Adams School of Dentistry, University of North Carolina, Chapel Hill, NC, USA Department of Orthodontics, Adams School of Dentistry, University of North Carolina, Chapel Hill, NC, USA

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Hickman HD, Moutsopoulos NM. Viral infection and antiviral immunity in the oral cavity. Nat Rev Immunol 2024:10.1038/s41577-024-01100-x. [PMID: 39533045 DOI: 10.1038/s41577-024-01100-x] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Accepted: 10/02/2024] [Indexed: 11/16/2024]

Machado L, Prudente R, Franco E, Gatto M, Mota G, Pagan L, Brizola L, dos Santos M, Cunha T, Sabino-Silva R, Goulart L, Martins M, Santos P, Maia L, Albuquerque A, Ferreira E, Baldi B, Okoshi M, Tanni S. Salivary Metabolomics in Patients with Long COVID-19 Infection. Metabolites 2024;14:598. [PMID: 39590834 PMCID: PMC11596941 DOI: 10.3390/metabo14110598] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 06/06/2024] [Revised: 08/24/2024] [Accepted: 10/17/2024] [Indexed: 11/28/2024] Open

Abstract

Background: Long COVID-19 has been characterized by the presence of symptoms lasting longer than 4 weeks after the acute infection. The pathophysiology of clinical manifestations still lacks knowledge. Objective: The objective of this paper was to evaluate metabolite abundance in the saliva of long COVID patients 60 days after hospital discharge. Methods: A convenience sample was composed of 30 post-discharge patients with long COVID and seven non-COVID-19 controls. All COVID-19 patients were evaluated by demographic characteristics, spirometry, 6 min walk test (6mWT), Saint George Respiratory Questionnaire (SGRQ), and body composition. Metabolomics was performed on saliva. Results: The long COVID-19 patients were 60.4 ± 14.3 years-old, and 66% male. Their lean body mass was 30.7 ± 7.3 kg and fat mass, 34.4 ± 13.7 kg. Spirometry evaluation showed forced vital capacity (FVC) of 3.84 ± 0.97 L with 96.0 ± 14.0% of the predicted value, and forced expiratory volume in the first second (FEV1) of 3.11 ± 0.83 L with 98.0 ± 16.0 of the predicted value. The long COVID-19 patients had reduced maximal inspiratory (90.1 ± 31.6 cmH2O) and maximal expiratory (97.3 ± 31.0 cmH2O) pressures. SGRQ showed domain symptoms of 32.3 ± 15.2, domain activities of 41.9 ± 25.6, and domain impact 13.7 ± 11.4, with a mean of 24.3 ± 14.9%. Physical capacity measured by distance covered in the 6mWT was 418.2 ± 130 m with a 73.3% (22.3-98.1) predictive value. The control group consisted of 44.1 ± 10.7-year-old men with a body mass index of 26.5 ± 1.66 Kg/m2. Metabolomics revealed 19 differentially expressed metabolites; expression was lower in 16 metabolites, and 2 metabolites were absent in the COVID-19 patients compared to controls. Calenduloside G methyl ester (p = 0.03), Gly Pro Lys (p = 0.0001), and creatine (p = 0.0001) expressions were lower in patients than controls. Conclusions: Long COVID-19 patients present less abundance of calenduloside G methyl ester, Gly Pro Lys, and creatine in saliva than healthy controls. Lower creatine abundance may be related to reduced physical capacity and fatigue.

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Affiliation(s)

Luiz Machado Botucatu Medical School, São Paulo State University, Botucatu 18618-970, Brazil; (R.P.); (E.F.); (M.G.); (G.M.); (L.P.); (L.B.); (M.d.S.); (M.O.); (S.T.)
Robson Prudente Botucatu Medical School, São Paulo State University, Botucatu 18618-970, Brazil; (R.P.); (E.F.); (M.G.); (G.M.); (L.P.); (L.B.); (M.d.S.); (M.O.); (S.T.)
Estefânia Franco Botucatu Medical School, São Paulo State University, Botucatu 18618-970, Brazil; (R.P.); (E.F.); (M.G.); (G.M.); (L.P.); (L.B.); (M.d.S.); (M.O.); (S.T.)
Mariana Gatto Botucatu Medical School, São Paulo State University, Botucatu 18618-970, Brazil; (R.P.); (E.F.); (M.G.); (G.M.); (L.P.); (L.B.); (M.d.S.); (M.O.); (S.T.)
Gustavo Mota Botucatu Medical School, São Paulo State University, Botucatu 18618-970, Brazil; (R.P.); (E.F.); (M.G.); (G.M.); (L.P.); (L.B.); (M.d.S.); (M.O.); (S.T.)
Luana Pagan Botucatu Medical School, São Paulo State University, Botucatu 18618-970, Brazil; (R.P.); (E.F.); (M.G.); (G.M.); (L.P.); (L.B.); (M.d.S.); (M.O.); (S.T.)
Luís Brizola Botucatu Medical School, São Paulo State University, Botucatu 18618-970, Brazil; (R.P.); (E.F.); (M.G.); (G.M.); (L.P.); (L.B.); (M.d.S.); (M.O.); (S.T.)
Maércio dos Santos Botucatu Medical School, São Paulo State University, Botucatu 18618-970, Brazil; (R.P.); (E.F.); (M.G.); (G.M.); (L.P.); (L.B.); (M.d.S.); (M.O.); (S.T.)
Thulio Cunha Faculty of Medicine of the Federal, University of Uberlandia, Uberlândia 38408-100, Brazil; (T.C.); (R.S.-S.); (M.M.); (P.S.); (L.M.)
Robinson Sabino-Silva Faculty of Medicine of the Federal, University of Uberlandia, Uberlândia 38408-100, Brazil; (T.C.); (R.S.-S.); (M.M.); (P.S.); (L.M.)
Luiz Goulart Faculty of Medicine of the Federal, University of Uberlandia, Uberlândia 38408-100, Brazil; (T.C.); (R.S.-S.); (M.M.); (P.S.); (L.M.)
Mario Martins Faculty of Medicine of the Federal, University of Uberlandia, Uberlândia 38408-100, Brazil; (T.C.); (R.S.-S.); (M.M.); (P.S.); (L.M.)
Paula Santos Faculty of Medicine of the Federal, University of Uberlandia, Uberlândia 38408-100, Brazil; (T.C.); (R.S.-S.); (M.M.); (P.S.); (L.M.)
Larissa Maia Faculty of Medicine of the Federal, University of Uberlandia, Uberlândia 38408-100, Brazil; (T.C.); (R.S.-S.); (M.M.); (P.S.); (L.M.)
André Albuquerque Department of Pneumology, University of São Paulo, São Paulo 05403-000, Brazil; (A.A.); (B.B.)
Eloara Ferreira Department of the Federal, University of São Paulo, São Paulo 01246-903, Brazil;
Bruno Baldi Department of Pneumology, University of São Paulo, São Paulo 05403-000, Brazil; (A.A.); (B.B.)
Marina Okoshi Botucatu Medical School, São Paulo State University, Botucatu 18618-970, Brazil; (R.P.); (E.F.); (M.G.); (G.M.); (L.P.); (L.B.); (M.d.S.); (M.O.); (S.T.)
Suzana Tanni Botucatu Medical School, São Paulo State University, Botucatu 18618-970, Brazil; (R.P.); (E.F.); (M.G.); (G.M.); (L.P.); (L.B.); (M.d.S.); (M.O.); (S.T.)

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Qu B, Liu H, Zhu W, Wei M, Yin W, Liu Q, Zhu Z. Oral complications during the two waves of COVID-19 pandemic in China: a prospective cross-section analysis of 510 cases using questionnaire. BMC Oral Health 2024;24:1265. [PMID: 39443934 PMCID: PMC11515644 DOI: 10.1186/s12903-024-05058-z] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 07/10/2024] [Accepted: 10/14/2024] [Indexed: 10/25/2024] Open

Affiliation(s)

Bojing Qu State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, National Clinical Research Center for Oral Diseases, Shaanxi Clinical Research Center for Oral Diseases, Department of Oral medicine, School of Stomatology, Xi'an, 710032, Shaanxi, China
Honglin Liu State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, National Clinical Research Center for Oral Diseases, Shaanxi Clinical Research Center for Oral Diseases, Department of Oral medicine, School of Stomatology, Xi'an, 710032, Shaanxi, China
Wanli Zhu State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, National Clinical Research Center for Oral Diseases, Shaanxi Clinical Research Center for Oral Diseases, Department of Oral medicine, School of Stomatology, Xi'an, 710032, Shaanxi, China
Minghui Wei State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, National Clinical Research Center for Oral Diseases, Shaanxi Clinical Research Center for Oral Diseases, Department of Oral medicine, School of Stomatology, Xi'an, 710032, Shaanxi, China
Wen Yin Department of Microbiology & Pathogen Biology, School of Basic Medical Sciences, Fourth Military Medical University, Xi'an, 710032, Shaanxi, China Department of Transfusion Medicine, Xijing Hospital, Fourth Military Medical University, Xi'an, 710032, Shaanxi, China
Qing Liu State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, National Clinical Research Center for Oral Diseases, Shaanxi Clinical Research Center for Oral Diseases, Department of Oral medicine, School of Stomatology, Xi'an, 710032, Shaanxi, China. Department of Oral medicine, School of Stomatology, the Fourth Military Medical University, Xi'an, 710032, Shaanxi Province, P. R. China.
Zhenlai Zhu State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, National Clinical Research Center for Oral Diseases, Shaanxi Clinical Research Center for Oral Diseases, Department of Oral medicine, School of Stomatology, Xi'an, 710032, Shaanxi, China. Department of Microbiology & Pathogen Biology, School of Basic Medical Sciences, Fourth Military Medical University, Xi'an, 710032, Shaanxi, China. Department of Transfusion Medicine, Xijing Hospital, Fourth Military Medical University, Xi'an, 710032, Shaanxi, China. Department of Oral medicine, School of Stomatology, the Fourth Military Medical University, Xi'an, 710032, Shaanxi Province, P. R. China.

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Bontempo A, Chirino A, Heidari A, Lugo A, Shindo S, Pastore MR, Madonia R, Antonson SA, Godoy C, Nichols FC, Potempa J, Davey ME, Kawai T, Cayabyab MJ. Inhibition of SARS-CoV-2 infection by Porphyromonas gingivalis and the oral microbiome. Microbiol Spectr 2024;12:e0059924. [PMID: 39162507 PMCID: PMC11448423 DOI: 10.1128/spectrum.00599-24] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/08/2024] [Accepted: 07/10/2024] [Indexed: 08/21/2024] Open

Abstract

The COVID-19 pandemic persists despite the availability of vaccines, and it is, therefore, crucial to develop new therapeutic and preventive approaches. In this study, we investigated the potential role of oral microbiome in SARS-CoV-2 infection. Using an in vitro SARS-CoV-2 pseudovirus infection assay, we found a potent inhibitory effect exerted by Porphyromonas gingivalis on SARS-CoV-2 infection mediated by known P. gingivalis compounds such as phosphoglycerol dihydroceramide (PGDHC) and gingipains as well as by unknown bacterial factors. We found that the gingipain-mediated inhibition of infection is likely due to cytotoxicity, whereas PGDHC inhibited virus infection by an unknown mechanism. Unidentified factors present in P. gingivalis supernatant inhibited SARS-CoV-2 likely via the fusion step of the virus life cycle. We addressed the role of other oral bacteria and found certain periodontal pathogens capable of inhibiting SARS-CoV-2 pseudovirus infection by inducing cytotoxicity on target cells. In the human oral cavity, we observed that the modulatory activity of oral microbial communities varied among individuals, in that some saliva-based cultures were capable of inhibiting while others were enhancing infection. These findings contribute to our understanding of the complex relationship between the oral microbiome and viral infections, offering potential avenues for innovative therapeutic strategies in combating COVID-19.

IMPORTANCE

The oral microbiome is important in health and disease, and in this study, we addressed the potential role of the oral microbiome in COVID-19 infection. Our in vitro studies suggest that certain bacteria of the oral microbiome such as P. gingivalis produce compounds that could potentially inhibit SARS-CoV-2 infection. These findings elucidating the interactions between the oral microbiome and SARS-CoV-2 infection will be important in our understanding of COVID-19 pathogenesis and the development of innovative therapeutic and preventive strategies against COVID-19 infection.

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Affiliation(s)

Alexander Bontempo Department of Oral Science and Translational Research, Health Professions Division, College of Dental Medicine, Nova Southeastern University, Fort Lauderdale, Florida, USA
Alexandra Chirino Department of Oral Science and Translational Research, Health Professions Division, College of Dental Medicine, Nova Southeastern University, Fort Lauderdale, Florida, USA
Alireza Heidari Department of Oral Science and Translational Research, Health Professions Division, College of Dental Medicine, Nova Southeastern University, Fort Lauderdale, Florida, USA
Alexandra Lugo Department of Oral Science and Translational Research, Health Professions Division, College of Dental Medicine, Nova Southeastern University, Fort Lauderdale, Florida, USA
Satoru Shindo Department of Oral Science and Translational Research, Health Professions Division, College of Dental Medicine, Nova Southeastern University, Fort Lauderdale, Florida, USA
Maria R. Pastore Department of Oral Science and Translational Research, Health Professions Division, College of Dental Medicine, Nova Southeastern University, Fort Lauderdale, Florida, USA
Riccardo Madonia Department of Oral Science and Translational Research, Health Professions Division, College of Dental Medicine, Nova Southeastern University, Fort Lauderdale, Florida, USA
Sibel A. Antonson Department of Prosthodontics, College of Dental Medicine, Nova Southeastern University, Fort Lauderdale, Florida, USA Department of Prosthodontics, Biruni University, Istanbul, Turkey
Cristina Godoy Department of Oral Science and Translational Research, Health Professions Division, College of Dental Medicine, Nova Southeastern University, Fort Lauderdale, Florida, USA
Frank C. Nichols Department of Periodontology, University of Connecticut School of Dental Medicine, Farmington, Connecticut, USA
Jan Potempa Department of Oral Immunology and Infectious Diseases, University of Louisville School of Dentistry, Louisville, Kentucky, USA Department of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland
Mary Ellen Davey Department of Microbiology, ADA Forsyth Institute, Cambridge, Massachusetts, USA
Toshihisa Kawai Department of Oral Science and Translational Research, Health Professions Division, College of Dental Medicine, Nova Southeastern University, Fort Lauderdale, Florida, USA
Mark J. Cayabyab Department of Oral Science and Translational Research, Health Professions Division, College of Dental Medicine, Nova Southeastern University, Fort Lauderdale, Florida, USA

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da Fonseca Orcina B, Bertin L, Izu Nakamura Pietro EC, Pescinelli Garcia Kuroda J, Marques da Costa Alves L, Vieira Vilhena F, da Silva Santos PS. Can the use of iron phthalocyanine-derivative mouthrinses in COVID-19 patients provide systemic benefits? Research into this potential should be considered. GMS HYGIENE AND INFECTION CONTROL 2024;19:Doc45. [PMID: 39553294 PMCID: PMC11565403 DOI: 10.3205/dgkh000500] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Figures] [Subscribe] [Scholar Register] [Indexed: 11/19/2024]

Porat Ben Amy D, Rohana H, Azrad M, V Joachim M, Bar O, Abu El-Naaj I, Peretz A. Exploring the relationship between cycle threshold values and oral manifestations in COVID-19: a comprehensive overview. Acta Odontol Scand 2024;83:516-521. [PMID: 39311869 PMCID: PMC11443029 DOI: 10.2340/aos.v83.41390] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 01/27/2024] [Accepted: 07/28/2024] [Indexed: 10/04/2024]

Rohana H, Marmur G, Azrad M, Peretz A. A new antigen test device for rapid influenza A and B detection. Heliyon 2024;10:e33979. [PMID: 39055805 PMCID: PMC11269840 DOI: 10.1016/j.heliyon.2024.e33979] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 11/19/2023] [Revised: 06/30/2024] [Accepted: 07/01/2024] [Indexed: 07/28/2024] Open

Peskersoy C, Oguzhan A, Akcay C, Dincturk BA, Can HS, Kamer EK, Haciyanli M. Evaluation of oral health status and immunological parameters of hospitalized COVID-19 patients during acute and recovery phases: A randomized clinical trial. J Dent Sci 2024;19:1515-1524. [PMID: 39035327 PMCID: PMC11259628 DOI: 10.1016/j.jds.2024.01.022] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Journal Information] [Subscribe] [Scholar Register] [Received: 01/13/2024] [Revised: 01/27/2024] [Indexed: 07/23/2024] Open

Abstract

Background/purpose

It is known that COVID-19 patients show many clinical oral symptoms due to the immunological mechanisms triggered by the virus. Aim of this study is to analyze the antibody response to SARS-CoV-2, and to evaluate the oral health status of hospitalized patients.

Materials and methods

160 patients with COVID-19 confirmed by SARS-CoV-2-specific RT-PCR testing and 160 healthy volunteers (HI) with similar age, gender and systemic status were included to compare the bio-chemical and oral manifestations. Oropharyngeal swab specimens were collected to evaluate the salivary interleukins (IL-1, IL-6, IL-10) and immunoglobulins (sIgA, sIgG, sIgM). Oral findings (DMFT, plaque index, salivary flow rate), socio-demographic information and systemic conditions were also recorded. Chi-square, Mann-Whitney U and Spearman's ratio tests were applied to determine the possible correlations between the factors (P = 0.05).

Results

The mean DMFT scores of COVID-19 patients (12.71 ± 7.3) were significantly higher than the HI (7.39 ± 2.8), whereas cases of total or partial edentulism were more common among COVID-19 patients (P < 0.05). While plaque index scores were similar for both groups (P > 0.05), salivary parameters were found statistically different (P < 0.05). Severe and moderate cases showed higher proinflammatory interleukin levels (IL-1 = 68.74 pg/ml, IL-6 = 53.31 pg/ml) amongst all (P < 0.05). While secretory immunoglobulins were almost depleted at baseline, (sIgA = 0.11 mg/ml, sIgG = 0.21 mg/ml, sIgM = 0.08 mg/ml) they reached to threshold levels after 4 weeks.

Conclusion

Higher proinflammatory interleukin levels indicated that traces of ongoing "Cytokine Storm" in COVID-19 patients which can also be observed in oral environment. Poor oral hygiene and malnutrition due to edentulism can pave the way for having a severe COVID-19 infection.

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Sulistyani LD, Julia V, Soeprapto A, Swari RP, Rosmanato F, Haryanto B, Cahyarini C, Panjaitan R, Maharani DA. The effects of mouth rinsing and gargling with mouthwash containing povidone-iodine and hydrogen peroxide on the cycle threshold value of Severe Acute Respiratory Syndrome Coronavirus 2: A randomized controlled trial of asymptomatic and mildly symptomatic patients. F1000Res 2024;11:1238. [PMID: 38988895 PMCID: PMC11234079 DOI: 10.12688/f1000research.110843.2] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Figures] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Accepted: 04/23/2024] [Indexed: 07/12/2024] Open

Alkharobi HE, Alzahrani MM, Bamashmous S, Alghamdi A. Oral symptoms potentially associated with mild-to-moderate COVID-19 in tobacco users. Tob Induc Dis 2024;22:TID-22-75. [PMID: 38742218 PMCID: PMC11089344 DOI: 10.18332/tid/186531] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 02/14/2024] [Revised: 03/24/2024] [Accepted: 03/27/2024] [Indexed: 05/16/2024] Open

Abstract

INTRODUCTION

Coronavirus disease (COVID-19) is a worldwide infection characterized by various symptoms. Few studies have examined its oral manifestations. However, there is insufficient information on the oral manifestations of patients with COVID-19 who use tobacco products. Therefore, this cross-sectional study investigated oral symptoms of tobacco-using patients with mild-to-moderate COVID-19.

METHODS

This study used a convenience sample of non-hospitalized patients (aged ≥18 years) with mild-to-moderate COVID-19 diagnosed by polymerized chain reaction (PCR). This study excluded pregnant or lactating women or patients with serious COVID-19 complications, including those who required hospitalization or were on specific medications (antiviral, corticosteroid, antimicrobial, or immunosuppressive). Oral examinations were performed, including labial, buccal, and gingival mucosa, tongue, floor of the mouth, and palate, for any newly developed lesions associated with the onset of COVID-19. The salivary flow was determined using the passive drool collection technique.

RESULTS

Lip dryness, gingivitis, tongue lesions, and taste loss were the most commonly reported oral symptoms in patients with mild-to-moderate COVID-19. The most common general symptoms were tiredness and headache (63.9%), followed by dry cough, myalgia, sore throat, and fever. This study found 139 occurrences of oral symptoms, of which 52 were dry lips (27 tobacco non-users, and 25 tobacco users), and 11 were gingivitis (five non-users, and six tobacco users), and 12 tongue changes (eight non-users, and four tobacco users). Ageusia, or loss of taste sensation, was most commonly reported with or without other oral COVID-19 symptoms (55 occurrences: 36 non-users and 19 tobacco users). No significant differences were found in oral symptoms between tobacco non-users and tobacco users.

CONCLUSIONS

There is a need to expand the routine examination protocol for patients during future respiratory pandemics, as monitoring oral health allows dentists to improve the management of oral sequelae during a pandemic.

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Campeanu AT, Rus M, Ispas S, Herlo A, Craciun A, Ionescu C, Banariu GM, Cambrea CS, Nicolae M, Nelson-Twakor A, Dumitru IM. Prevalence and association of oral manifestations with disease severity in patients diagnosed with COVID‑19: A cross‑sectional study. MEDICINE INTERNATIONAL 2024;4:30. [PMID: 38660127 PMCID: PMC11040283 DOI: 10.3892/mi.2024.154] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Figures] [Subscribe] [Scholar Register] [Received: 02/04/2024] [Accepted: 03/26/2024] [Indexed: 04/26/2024]

Abstract

Systemic disorders may exhibit early signs when conducting an oral examination. Since the onset of the COVID-19 pandemic, several studies have been published detailing the direct impact of the virus on the oral cavity. The present study aimed to determine whether indeed there are any significant disparities in oropharyngeal manifestations between individuals infected with severe acute respiratory syndrome coronavirus 2 and a control group, and whether the virus has the ability to invade and reproduce inside oral keratinocytes and fibroblasts, resulting in the development of oral ulcerations and superficial lesions. The present study provides an overview of the symptoms that occur at an early stage of the illness, and the most commonly affected regions of the oral cavity, including the tongue, lips, palate and oropharynx are examined. In the present retrospective study, 52 patients infected with COVID-19 were recruited between April, 2021 and October, 2022. In addition, 52 individuals who tested negative for the virus were recruited as the control group. The study was conducted through a thorough examination and questionnaire provided to all participants. The results revealed that among the cohort of patients from the COVID-19 group examined (n=52), a proportion (mean, 16.15) displayed oral manifestations. Specifically, 75% of the patients in the COVID-19 group described oral cavity pain, and 69% of these patients had changes in teeth color or dental caries. In summary, in relation to the control group, the prevalence of oropharyngeal symptoms was generally lower compared to the COVID-19 group, apart from oral cavity pain (30.8%), tonsillitis (17.3%), bleeding (34.6%), teeth color changes (36.5%), recurrence (15.4%) and abscesses (7.7%). Thus, on the whole, the patients without COVID-19 had fewer oral manifestations.

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He J, Mao N, Lyu W, Zhou S, Zhang Y, Liu Z, Xu Z. Association between oral microbiome and five types of respiratory infections: a two-sample Mendelian randomization study in east Asian population. Front Microbiol 2024;15:1392473. [PMID: 38659993 PMCID: PMC11039966 DOI: 10.3389/fmicb.2024.1392473] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 02/27/2024] [Accepted: 03/25/2024] [Indexed: 04/26/2024] Open

Prakash K, Bindu R, Sanoj NM. Prevalence of Oral Manifestations in COVID-19-Diagnosed Patients at a Tertiary Care Hospital in Kerala. J Maxillofac Oral Surg 2024;23:296-300. [PMID: 38601253 PMCID: PMC11001806 DOI: 10.1007/s12663-023-02049-5] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 06/28/2023] [Accepted: 10/21/2023] [Indexed: 04/12/2024] Open

Bernardo RA, Roque JV, de Oliveira Júnior CI, Lima NM, Machado LS, Duarte GRM, Costa NL, Sorgi CA, Soares FFL, Vaz BG, Chaves AR. Exploring salivary lipid profile changes in COVID-19 patients: Insights from mass spectrometry analysis. Talanta 2024;269:125522. [PMID: 38091738 DOI: 10.1016/j.talanta.2023.125522] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/27/2023] [Revised: 11/27/2023] [Accepted: 12/04/2023] [Indexed: 01/05/2024]

Heydari B, Sahebnasagh A, Omrani MA, Azimi S, Dehghani MH, Salehi-Abargouei A, Farman F, Saghafi F. Promises and Pitfalls of Calcineurin Inhibitors in COVID-19: A Systematic Review and Meta-analysis of Controlled Trials. Curr Med Chem 2024;31:4745-4755. [PMID: 38099537 DOI: 10.2174/0109298673264362231022150520] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 05/27/2023] [Revised: 06/24/2023] [Accepted: 07/31/2023] [Indexed: 01/23/2025]

Nawab A, Acosta A, Levine CG, Hoffer ME, Casiano R, Liu XZ. Chemosensory function recovery in COVID-19 patients: A cross-sectional study. Am J Otolaryngol 2024;45:104047. [PMID: 37738881 DOI: 10.1016/j.amjoto.2023.104047] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [MESH Headings] [Grants] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 06/27/2023] [Accepted: 09/12/2023] [Indexed: 09/24/2023]

Jung T, Choi BY, Jang M, Kim T, Seo E, Kim JK. Comparative Analysis of Olfactory and Gustatory Function of Patients With COVID-19 Olfactory Dysfunction and Non-COVID-19 Postinfectious Olfactory Dysfunction. J Korean Med Sci 2023;38:e352. [PMID: 37935167 PMCID: PMC10627727 DOI: 10.3346/jkms.2023.38.e352] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 03/20/2023] [Accepted: 07/18/2023] [Indexed: 11/09/2023] Open

Abstract

BACKGROUND

Coronavirus disease 2019 (COVID-19) is known to have a high incidence of loss of smell and taste. However, studies in the early stages of the COVID-19 pandemic have evaluated these symptoms using subjective surveys and simple olfactory tests only. Hence, we compared the olfactory and gustatory characteristics of patient groups with COVID-19 olfactory dysfunction (C19OD) and non-COVID-19 postinfectious olfactory dysfunction (PIOD) using an objective olfactory test and evaluated the significance of olfactory training in both patient groups.

METHODS

We retrospectively analyzed the medical records of 14 patients with a decreased sense of smell after having positive COVID-19 polymerase chain reaction results, and 56 patients with PIOD with no history of confirmed COVID-19. Participants were evaluated using the Korean version of the Sniffin' stick (KVSS) II, and chemical gustometry and olfactory training was assessed during their first visit. Olfactory training was then re-evaluated after an average of 8 (± 6) weeks.

RESULTS

The average age of participants in the C19OD group was lower than in those in the non-COVID-19 PIOD group. The proportion of men in the C19OD group was higher than in the non-COVID-19 PIOD group. At baseline assessment, the C19OD group had better olfactory and gustatory functions. After olfactory training, the non-COVID-19 PIOD patient group showed a significant increase in all KVSS II Total, T, D, and I scores, but there was a non-significant increase in all scores in the C19OD group.

CONCLUSION

The C19OD group had better olfactory and gustatory function than the non-COVID-19 PIOD group at the initial assessment. After olfactory training, there was an increase in olfactory function test scores in both groups. Olfactory training may be helpful in C19OD, as in non-COVID-19 PIOD.

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Gupta A, Shrivastav K, Agrawal A, Purohit A, Chanchlani R. Estimating the prevalence of oral manifestations in COVID-19 patients: a systematic review. Osong Public Health Res Perspect 2023;14:388-417. [PMID: 37920896 PMCID: PMC10626325 DOI: 10.24171/j.phrp.2023.0033] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 02/06/2023] [Revised: 06/09/2023] [Accepted: 07/10/2023] [Indexed: 11/04/2023] Open

Lin SY, Sun JS, Hung MC, Chang JZC. Effectiveness of mouth rinses against COVID-19: a systematic review and network meta-analysis. J Hosp Infect 2023;139:175-191. [PMID: 37419189 DOI: 10.1016/j.jhin.2023.06.022] [Citation(s) in RCA: 5] [Impact Index Per Article: 2.5] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/29/2023] [Revised: 06/21/2023] [Accepted: 06/25/2023] [Indexed: 07/09/2023]

Tsuchiya H. COVID-19 Oral Sequelae: Persistent Gustatory and Saliva Secretory Dysfunctions after Recovery from COVID-19. Med Princ Pract 2023;32:166-177. [PMID: 37271130 PMCID: PMC10601698 DOI: 10.1159/000531373] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 10/27/2022] [Accepted: 05/21/2023] [Indexed: 06/06/2023] Open

Hussein RR, Ahmed E, Abou-Bakr A, El-Gawish AA, Ras ABE, Ghalwash DM. Oral Changes in Hospitalized COVID-19 Patients: A Cross-Sectional Multicentric Study. Int J Dent 2023;2023:3002034. [PMID: 37223395 PMCID: PMC10202601 DOI: 10.1155/2023/3002034] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 04/05/2022] [Revised: 05/19/2022] [Accepted: 05/26/2022] [Indexed: 05/25/2023] Open

Hamed SA. Post-COVID-19 persistent olfactory, gustatory, and trigeminal chemosensory disorders: Definitions, mechanisms, and potential treatments. World J Otorhinolaryngol 2023;10:4-22. [DOI: 10.5319/wjo.v10.i2.4] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.5] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 01/20/2023] [Revised: 03/28/2023] [Accepted: 04/18/2023] [Indexed: 05/08/2023] Open

Agyekum BA, Tuglo LS. Effect of COVID-19 on dental care for children during the post-pandemic era in Ghana: a concern for policymaking. FRONTIERS IN DENTAL MEDICINE 2023;4:1120685. [PMID: 39916913 PMCID: PMC11797804 DOI: 10.3389/fdmed.2023.1120685] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.5] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 12/13/2022] [Accepted: 03/17/2023] [Indexed: 02/09/2025] Open

Beltrán EO, Castellanos JE, Corredor ZL, Morgado W, Zarta OL, Cortés A, Avila V, Martignon S. Tracing ΦX174 bacteriophage spreading during aerosol-generating procedures in a dental clinic. Clin Oral Investig 2023:10.1007/s00784-023-04937-z. [PMID: 36933045 PMCID: PMC10024015 DOI: 10.1007/s00784-023-04937-z] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Journal Information] [Subscribe] [Scholar Register] [Received: 01/14/2023] [Accepted: 02/28/2023] [Indexed: 03/19/2023]

Abstract

OBJECTIVE

The aim of this study was to test the plausibility of using the ΦX174 bacteriophage as a tracer of viral aerosols spreading in a dental aerosol-generating procedure (AGP) model.

METHODS

ΦX174 bacteriophage (~ 10⁸ plaque-forming units (PFU)/mL) was added into instrument irrigation reservoirs and aerosolized during class-IV cavity preparations followed by composite fillings on natural upper-anterior teeth (n = 3) in a phantom head. Droplets/aerosols were sampled through a passive approach that consisted of Escherichia coli strain C600 cultures immersed in a LB top agar layer in Petri dishes (PDs) in a double-layer technique. In addition, an active approach consisted of E coli C600 on PDs sets mounted in a six-stage cascade Andersen impactor (AI) (simulating human inhalation). The AI was located at 30 cm from the mannequin during AGP and afterwards at 1.5 m. After collection PDs were incubated overnight (18 h at 37 °C) and bacterial lysis was quantified.

RESULTS

The passive approach disclosed PFUs mainly concentrated over the dental practitioner, on the mannequin's chest and shoulder and up to 90 cm apart, facing the opposite side of the AGP's source (around the spittoon). The maximum aerosol spreading distance was 1.5 m in front of the mannequin's mouth. The active approach disclosed collection of PFUs corresponding to stages (and aerodynamic diameters) 5 (1.1-2.1 µm) and 6 (0.65-1.1 µm), mimicking access to the lower respiratory airways.

CONCLUSION

The ΦX174 bacteriophage can be used as a traceable viral surrogate in simulated studies contributing to understand dental bioaerosol's behavior, its spreading, and its potential threat for upper and lower respiratory tract.

CLINICAL RELEVANCE

The probability to find infectious virus during AGPs is high. This suggests the need to continue characterizing the spreading viral agents in different clinical settings through combination of passive and active approaches. In addition, subsequent identification and implementation of virus-related mitigation strategies is relevant to avoid occupational virus infections.

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Xu P, Chen J, Qian C, Yu W. Pre-enriched saline gargle samples for detection of SARS-CoV-2. Biosci Trends 2023;17:81-84. [PMID: 36653152 DOI: 10.5582/bst.2022.01530] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 01/18/2023]

Kim JG, Zhang A, Rauseo AM, Goss CW, Mudd PA, O'Halloran JA, Wang L. The salivary and nasopharyngeal microbiomes are associated with SARS-CoV-2 infection and disease severity. J Med Virol 2023;95:e28445. [PMID: 36583481 PMCID: PMC9880756 DOI: 10.1002/jmv.28445] [Citation(s) in RCA: 9] [Impact Index Per Article: 4.5] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 10/24/2022] [Revised: 12/15/2022] [Accepted: 12/26/2022] [Indexed: 12/31/2022]

Bontempo A, Chirino A, Heidari A, Boparai S, Arora S, Ruiz S, Antonson SA, Kawai T, Cayabyab MJ. Assessment of SARS-CoV-2 entry in gingival epithelial cells expressing CD147. Eur J Oral Sci 2023;131:e12906. [PMID: 36412995 DOI: 10.1111/eos.12906] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 05/14/2022] [Accepted: 10/31/2022] [Indexed: 11/23/2022]

Similar Sensitivity of SARS-CoV-2 Detection in Oropharyngeal/Nasopharyngeal and Saliva Samples on the Hologic Panther Platform. Diagnostics (Basel) 2023;13:diagnostics13030347. [PMID: 36766452 PMCID: PMC9914531 DOI: 10.3390/diagnostics13030347] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 12/07/2022] [Revised: 01/02/2023] [Accepted: 01/05/2023] [Indexed: 01/19/2023] Open

SARS-CoV-2 Detection via RT-PCR in Matched Saliva and Nasopharyngeal Samples Reveals High Concordance in Different Commercial Assays. Diagnostics (Basel) 2023;13:diagnostics13020329. [PMID: 36673140 PMCID: PMC9857954 DOI: 10.3390/diagnostics13020329] [Citation(s) in RCA: 3] [Impact Index Per Article: 1.5] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 10/28/2022] [Revised: 12/05/2022] [Accepted: 12/21/2022] [Indexed: 01/18/2023] Open

RT-LAMP Multicenter Study for SARS-CoV-2 Genome Molecular Detection in Brazilian Swab and Saliva Samples. Diagnostics (Basel) 2023;13:diagnostics13020210. [PMID: 36673025 PMCID: PMC9858473 DOI: 10.3390/diagnostics13020210] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 11/25/2022] [Revised: 12/27/2022] [Accepted: 01/03/2023] [Indexed: 01/11/2023] Open

Lin W, Gao F, Wang X, Qin N, Chen X, Tam KY, Zhang C, Zhang M, Sha O. The oral manifestations and related mechanisms of COVID-19 caused by SARS-CoV-2 infection. Front Cell Neurosci 2023;16:1006977. [PMID: 36687524 PMCID: PMC9846147 DOI: 10.3389/fncel.2022.1006977] [Citation(s) in RCA: 6] [Impact Index Per Article: 3.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 07/29/2022] [Accepted: 12/13/2022] [Indexed: 01/05/2023] Open

Pittman TW, Decsi DB, Punyadeera C, Henry CS. Saliva-based microfluidic point-of-care diagnostic. Theranostics 2023;13:1091-1108. [PMID: 36793864 PMCID: PMC9925318 DOI: 10.7150/thno.78872] [Citation(s) in RCA: 27] [Impact Index Per Article: 13.5] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/13/2022] [Accepted: 01/11/2023] [Indexed: 02/04/2023] Open

Hoseinzadeh M, Sa’adAbadi Z, Maleki Kambakhsh S, Babazadeh S. Dentists' lived experience of providing dental care during the COVID-19 pandemic: A qualitative study in Mashhad, Iran. FRONTIERS IN ORAL HEALTH 2023;4:1095240. [PMID: 36937504 PMCID: PMC10015384 DOI: 10.3389/froh.2023.1095240] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 11/15/2022] [Accepted: 01/31/2023] [Indexed: 03/05/2023] Open

Muacevic A, Adler JR, Poothakulath Krishnan R. Systematic Review and Meta-Analysis on Angiotensin Converting Enzyme 2 in Head and Neck Region. Cureus 2023;15:e33673. [PMID: 36819393 PMCID: PMC9928136 DOI: 10.7759/cureus.33673] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Accepted: 01/11/2023] [Indexed: 01/13/2023] Open

Pinzariu AC, Sova IA, Maranduca MA, Filip N, Drochioi IC, Vamesu CG, Clim A, Hurjui LL, Moscalu M, Soroceanu RP, Serban DN, Serban IL. Vitamin D Deficiency in Both Oral and Systemic Manifestations in SARS-CoV-2 Infection: Updated Review. Medicina (B Aires) 2022;59:medicina59010068. [PMID: 36676692 PMCID: PMC9866644 DOI: 10.3390/medicina59010068] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 11/22/2022] [Revised: 12/20/2022] [Accepted: 12/23/2022] [Indexed: 12/29/2022] Open

Affiliation(s)

Alin Constantin Pinzariu Department of Morpho-Functional Sciences II, Discipline of Physiology, “Grigore T. Popa” University of Medicine and Pharmacy, 700115 Iasi, Romania
Ivona Andreea Sova IOSUD Faculty of Medicine, “Grigore T. Popa” University of Medicine and Pharmacy, 700115 Iasi, Romania
Minela Aida Maranduca Department of Morpho-Functional Sciences II, Discipline of Physiology, “Grigore T. Popa” University of Medicine and Pharmacy, 700115 Iasi, Romania Internal Medicine Clinic, “St. Spiridon” County Clinical Emergency Hospital, 700115 Iasi, Romania
Nina Filip Department of Morpho-Functional Sciences II, Discipline of Biochemistry, “Grigore T. Popa” University of Medicine and Pharmacy, 700115 Iasi, Romania
Ilie Cristian Drochioi Department of Oral and Maxillofacial Surgery and Reconstructive, Faculty of Dental Medicine, “Grigore T. Popa” University of Medicine and Pharmacy, 700020 Iasi, Romania
Calin George Vamesu Department of Morpho-Functional Sciences II, Discipline of Physiology, “Grigore T. Popa” University of Medicine and Pharmacy, 700115 Iasi, Romania
Andreea Clim Department of Morpho-Functional Sciences II, Discipline of Physiology, “Grigore T. Popa” University of Medicine and Pharmacy, 700115 Iasi, Romania Correspondence: (A.C.); (M.M.)
Loredana Liliana Hurjui Department of Morpho-Functional Sciences II, Discipline of Physiology, “Grigore T. Popa” University of Medicine and Pharmacy, 700115 Iasi, Romania
Mihaela Moscalu Department of Preventive Medicine and Interdisciplinarity, “Grigore T. Popa” University of Medicine and Pharmacy, 700115 Iasi, Romania Correspondence: (A.C.); (M.M.)
Radu Petru Soroceanu Department of Surgery I, Discipline of Surgical Semiology, “Grigore T. Popa” University of Medicine and Pharmacy, 700115 Iasi, Romania
Dragomir Nicolae Serban Department of Morpho-Functional Sciences II, Discipline of Physiology, “Grigore T. Popa” University of Medicine and Pharmacy, 700115 Iasi, Romania
Ionela Lacramioara Serban Department of Morpho-Functional Sciences II, Discipline of Physiology, “Grigore T. Popa” University of Medicine and Pharmacy, 700115 Iasi, Romania

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Meller S, Al Khatri MSA, Alhammadi HK, Álvarez G, Alvergnat G, Alves LC, Callewaert C, Caraguel CGB, Carancci P, Chaber AL, Charalambous M, Desquilbet L, Ebbers H, Ebbers J, Grandjean D, Guest C, Guyot H, Hielm-Björkman A, Hopkins A, Kreienbrock L, Logan JG, Lorenzo H, Maia RDCC, Mancilla-Tapia JM, Mardones FO, Mutesa L, Nsanzimana S, Otto CM, Salgado-Caxito M, de los Santos F, da Silva JES, Schalke E, Schoneberg C, Soares AF, Twele F, Vidal-Martínez VM, Zapata A, Zimin-Veselkoff N, Volk HA. Expert considerations and consensus for using dogs to detect human SARS-CoV-2-infections. Front Med (Lausanne) 2022;9:1015620. [PMID: 36569156 PMCID: PMC9773891 DOI: 10.3389/fmed.2022.1015620] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Key Words] [Track Full Text] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/09/2022] [Accepted: 11/17/2022] [Indexed: 12/13/2022] Open

Affiliation(s)

Sebastian Meller Department of Small Animal Medicine & Surgery, University of Veterinary Medicine Hannover, Hanover, Germany
Maryam Sultan Ali Al Khatri Dubai Police K9, Dubai, United Arab Emirates
Hamad Khatir Alhammadi International Operations Department, Ministry of Interior of the United Arab Emirates, Abu Dhabi, United Arab Emirates
Guadalupe Álvarez Faculty of Veterinary Science, University of Buenos Aires, Buenos Aires, Argentina
Guillaume Alvergnat International Operations Department, Ministry of Interior of the United Arab Emirates, Abu Dhabi, United Arab Emirates
Lêucio Câmara Alves Department of Veterinary Medicine, Federal Rural University of Pernambuco, Recife, Brazil
Chris Callewaert Center for Microbial Ecology and Technology, Department of Biotechnology, Ghent University, Ghent, Belgium
Charles G. B. Caraguel School of Animal and Veterinary Sciences, The University of Adelaide, Roseworthy, SA, Australia
Paula Carancci Faculty of Veterinary Science, University of Buenos Aires, Buenos Aires, Argentina
Anne-Lise Chaber School of Animal and Veterinary Sciences, The University of Adelaide, Roseworthy, SA, Australia
Marios Charalambous Department of Small Animal Medicine & Surgery, University of Veterinary Medicine Hannover, Hanover, Germany
Loïc Desquilbet École Nationale Vétérinaire d’Alfort, IMRB, Université Paris Est, Maisons-Alfort, France
Hans Ebbers Kynoscience UG, Hörstel, Germany
Janek Ebbers Kynoscience UG, Hörstel, Germany
Dominique Grandjean École Nationale Vétérinaire d’Alfort, Université Paris-Est, Maisons-Alfort, France
Claire Guest Medical Detection Dogs, Milton Keynes, United Kingdom
Hugues Guyot Clinical Department of Production Animals, Fundamental and Applied Research for Animals & Health Research Unit, Faculty of Veterinary Medicine, University of Liège, Liège, Belgium
Anna Hielm-Björkman Department of Equine and Small Animal Medicine, University of Helsinki, Helsinki, Finland
Amy Hopkins Medical Detection Dogs, Milton Keynes, United Kingdom
Lothar Kreienbrock Department of Biometry, Epidemiology and Information Processing, University of Veterinary Medicine Hannover, Hanover, Germany
James G. Logan Department of Disease Control, London School of Hygiene and Tropical Medicine, London, United Kingdom Arctech Innovation, The Cube, Dagenham, United Kingdom
Hector Lorenzo Faculty of Veterinary Science, University of Buenos Aires, Buenos Aires, Argentina
Rita de Cassia Carvalho Maia Department of Veterinary Medicine, Federal Rural University of Pernambuco, Recife, Brazil
Juan M. Mancilla-Tapia Laboratory OBI K19 Biodetection Center, Hermosillo, Mexico
Fernando O. Mardones Escuela de Medicina Veterinaria, Facultad de Agronomía e Ingeniería Forestal and Facultad de Ciencias Biológicas y Facultad de Medicina, Pontificia Universidad Católica de Chile, Santiago, Chile
Leon Mutesa Center for Human Genetics, College of Medicine and Health Sciences, University of Rwanda, Kigali, Rwanda Rwanda National Joint Task Force COVID-19, Kigali, Rwanda
Sabin Nsanzimana University Teaching Hospital of Butare, Butare, Rwanda
Cynthia M. Otto Penn Vet Working Dog Center, Department of Clinical Sciences and Advanced Medicine, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA, United States
Marília Salgado-Caxito Escuela de Medicina Veterinaria, Facultad de Agronomía e Ingeniería Forestal and Facultad de Ciencias Biológicas y Facultad de Medicina, Pontificia Universidad Católica de Chile, Santiago, Chile
Florencia de los Santos Faculty of Veterinary Science, University of Buenos Aires, Buenos Aires, Argentina
Jeine Emanuele Santos da Silva Department of Animal Morphology and Physiology, Federal Rural University of Pernambuco, Recife, Brazil
Esther Schalke Bundeswehr Medical Service Headquarters, Koblenz, Germany
Clara Schoneberg Department of Biometry, Epidemiology and Information Processing, University of Veterinary Medicine Hannover, Hanover, Germany
Anísio Francisco Soares Department of Animal Morphology and Physiology, Federal Rural University of Pernambuco, Recife, Brazil
Friederike Twele Department of Small Animal Medicine & Surgery, University of Veterinary Medicine Hannover, Hanover, Germany
Victor Manuel Vidal-Martínez Laboratorio de Parasitología y Patología Acuática, Departamento de Recursos del Mar, Centro de Investigación y de Estudios Avanzados del IPN Unidad Mérida, Mérida, Yucatán, Mexico
Ariel Zapata Faculty of Veterinary Science, University of Buenos Aires, Buenos Aires, Argentina
Natalia Zimin-Veselkoff Escuela de Medicina Veterinaria, Facultad de Agronomía e Ingeniería Forestal and Facultad de Ciencias Biológicas y Facultad de Medicina, Pontificia Universidad Católica de Chile, Santiago, Chile
Holger A. Volk Department of Small Animal Medicine & Surgery, University of Veterinary Medicine Hannover, Hanover, Germany Center for Systems Neuroscience Hannover, Hanover, Germany

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Brahim Belhaouari D, Baudoin JP, Lagier JC, Monnet-Corti V, La Scola B, Antezack A. Microscopic observations of SARS-CoV-2 like particles in different oral samples. Eur J Oral Sci 2022;130:e12903. [PMID: 36404273 PMCID: PMC10099536 DOI: 10.1111/eos.12903] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 07/09/2022] [Accepted: 10/21/2022] [Indexed: 11/22/2022]

Development of Single-Cell Transcriptomics and Its Application in COVID-19. Viruses 2022;14:v14102271. [PMID: 36298825 PMCID: PMC9611071 DOI: 10.3390/v14102271] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/28/2022] [Revised: 09/30/2022] [Accepted: 10/11/2022] [Indexed: 11/07/2022] Open

Domínguez-Olmedo JL, Gragera-Martínez Á, Mata J, Pachón V. Age-Stratified Analysis of COVID-19 Outcome Using Machine Learning Predictive Models. Healthcare (Basel) 2022;10:2027. [PMID: 36292474 PMCID: PMC9601713 DOI: 10.3390/healthcare10102027] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/20/2022] [Revised: 10/06/2022] [Accepted: 10/11/2022] [Indexed: 11/04/2022] Open

Song C, Pan W, Brown B, Tang C, Huang Y, Chen H, Peng N, Wang Z, Weber D, Byrne-Steele M, Wu H, Liu H, Deng Y, He N, Li S. Immune repertoire analysis of normal Chinese donors at different ages. Cell Prolif 2022;55:e13311. [PMID: 35929064 DOI: 10.1111/cpr.13311] [Citation(s) in RCA: 4] [Impact Index Per Article: 1.3] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 04/25/2022] [Revised: 06/21/2022] [Accepted: 06/24/2022] [Indexed: 11/28/2022] Open

Oral dysbiosis and its linkage with SARS-CoV-2 infection. Microbiol Res 2022;261:127055. [PMID: 35597076 PMCID: PMC9065653 DOI: 10.1016/j.micres.2022.127055] [Citation(s) in RCA: 16] [Impact Index Per Article: 5.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 12/04/2021] [Revised: 04/21/2022] [Accepted: 04/26/2022] [Indexed: 01/21/2023]

Abstract

The human oral cavity harbours complex microbial communities with various commensal microorganisms that play pivotal roles in maintaining host health and immunity but can elicit local and systemic diseases. The role of commensal microorganisms in SARS-CoV-2 infection and disease susceptibility and enrichment of opportunistic pathobionts in the oral cavity is poorly understood. The present study aims to understand the altered landscape of the oral microbiome and mycobiome in SARS-CoV-2 infected patients (n = 30) and its correlation with risk factors compared to non-infected individuals (n = 24) using targeted amplicon sequencing. Diminution of species richness, an elevated abundance of opportunistic pathogens (Veillonella, Acinetobacter, Klebsiella, Prevotella, Gemella, and Streptococcus) and impaired metabolic pathways were observed in the COVID-19 patients. Similarly, altered oral mycobiome with enrichment of known respiratory disease causing pathogenic fungi were observed in the infected individuals. The data further suggested that reduction in immunomodulatory microorganisms lowers the protection of individuals from SARS-CoV-2. Linear discriminant analysis identified several differentially abundant taxa associated with risk factors (ageing and co-morbidities). We also observed distinct bacterial and fungal community structures of elderly infected patients compared to the younger age group members making them highly vulnerable to SARS-CoV-2 infection and disease severity. Furthermore, we also assessed the dynamics of the oral microbiome and mycobiome in symptomatic and asymptomatic patients, host types, co-morbidities, and viral load in the augmentation of specific pathobionts. Overall, the present study demonstrates the microbiome and mycobiome profiling of the COVID-19 infected individuals, the data further suggests that the SARS-CoV-2 infection triggers the prevalence of specific pathobiont.

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McPhillips L, MacSharry J. Saliva as an alternative specimen to nasopharyngeal swabs for COVID-19 diagnosis: Review. Access Microbiol 2022;4:acmi000366. [PMID: 36003360 PMCID: PMC9394527 DOI: 10.1099/acmi.0.000366] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 05/12/2021] [Accepted: 04/25/2022] [Indexed: 12/12/2022] Open

Tsukinoki K, Yamamoto T, Saito J, Sakaguchi W, Iguchi K, Inoue Y, Ishii S, Sato C, Yokoyama M, Shiraishi Y, Kato N, Shimada H, Makabe A, Saito A, Tanji M, Nagaoka I, Saruta J, Yamaguchi T, Kimoto S, Yamaguchi H. Prevalence of saliva immunoglobulin A antibodies reactive with severe acute respiratory syndrome coronavirus 2 among Japanese people unexposed to the virus. Microbiol Immunol 2022;66:403-410. [PMID: 35607844 PMCID: PMC9347685 DOI: 10.1111/1348-0421.13011] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/22/2022] [Revised: 05/11/2022] [Accepted: 05/21/2022] [Indexed: 11/29/2022]

Inchingolo AD, Malcangi G, Ceci S, Patano A, Corriero A, Vimercati L, Azzollini D, Marinelli G, Coloccia G, Piras F, Barile G, Settanni V, Mancini A, De Leonardis N, Garofoli G, Palmieri G, Isacco CG, Rapone B, Scardapane A, Curatoli L, Quaranta N, Ribezzi M, Massaro M, Jones M, Bordea IR, Tartaglia GM, Scarano A, Lorusso F, Macchia L, Larocca AMV, Aityan SK, Tafuri S, Stefanizzi P, Migliore G, Brienza N, Dipalma G, Favia G, Inchingolo F. Effectiveness of SARS-CoV-2 Vaccines for Short- and Long-Term Immunity: A General Overview for the Pandemic Contrast. Int J Mol Sci 2022;23:8485. [PMID: 35955621 PMCID: PMC9369331 DOI: 10.3390/ijms23158485] [Citation(s) in RCA: 6] [Impact Index Per Article: 2.0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 06/14/2022] [Revised: 07/22/2022] [Accepted: 07/27/2022] [Indexed: 11/22/2022] Open

Abstract

BACKGROUND

The recent COVID-19 pandemic produced a significant increase in cases and an emergency state was induced worldwide. The current knowledge about the COVID-19 disease concerning diagnoses, patient tracking, the treatment protocol, and vaccines provides a consistent contribution for the primary prevention of the viral infection and decreasing the severity of the SARS-CoV-2 disease. The aim of the present investigation was to produce a general overview about the current findings for the COVID-19 disease, SARS-CoV-2 interaction mechanisms with the host, therapies and vaccines' immunization findings.

METHODS

A literature overview was produced in order to evaluate the state-of-art in SARS-CoV-2 diagnoses, prognoses, therapies, and prevention.

RESULTS

Concerning to the interaction mechanisms with the host, the virus binds to target with its Spike proteins on its surface and uses it as an anchor. The Spike protein targets the ACE2 cell receptor and enters into the cells by using a special enzyme (TMPRSS2). Once the virion is quietly accommodated, it releases its RNA. Proteins and RNA are used in the Golgi apparatus to produce more viruses that are released. Concerning the therapies, different protocols have been developed in observance of the disease severity and comorbidity with a consistent reduction in the mortality rate. Currently, different vaccines are currently in phase IV but a remarkable difference in efficiency has been detected concerning the more recent SARS-CoV-2 variants.

CONCLUSIONS

Among the many questions in this pandemic state, the one that recurs most is knowing why some people become more seriously ill than others who instead contract the infection as if it was a trivial flu. More studies are necessary to investigate the efficiency of the treatment protocols and vaccines for the more recent detected SARS-CoV-2 variant.

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Affiliation(s)

Alessio Danilo Inchingolo Department of Interdisciplinary Medicine, Section of Dental Medicine, University of Bari “Aldo Moro”, 70124 Bari, Italy; (A.D.I.); (G.M.); (S.C.); (A.P.); (L.V.); (D.A.); (G.M.); (G.C.); (F.P.); (G.B.); (V.S.); (A.M.); (N.D.L.); (G.G.); (G.P.); (C.G.I.); (B.R.); (A.S.); (N.Q.); (M.J.); (G.D.); (G.F.)
Giuseppina Malcangi Department of Interdisciplinary Medicine, Section of Dental Medicine, University of Bari “Aldo Moro”, 70124 Bari, Italy; (A.D.I.); (G.M.); (S.C.); (A.P.); (L.V.); (D.A.); (G.M.); (G.C.); (F.P.); (G.B.); (V.S.); (A.M.); (N.D.L.); (G.G.); (G.P.); (C.G.I.); (B.R.); (A.S.); (N.Q.); (M.J.); (G.D.); (G.F.)
Sabino Ceci Department of Interdisciplinary Medicine, Section of Dental Medicine, University of Bari “Aldo Moro”, 70124 Bari, Italy; (A.D.I.); (G.M.); (S.C.); (A.P.); (L.V.); (D.A.); (G.M.); (G.C.); (F.P.); (G.B.); (V.S.); (A.M.); (N.D.L.); (G.G.); (G.P.); (C.G.I.); (B.R.); (A.S.); (N.Q.); (M.J.); (G.D.); (G.F.)
Assunta Patano Department of Interdisciplinary Medicine, Section of Dental Medicine, University of Bari “Aldo Moro”, 70124 Bari, Italy; (A.D.I.); (G.M.); (S.C.); (A.P.); (L.V.); (D.A.); (G.M.); (G.C.); (F.P.); (G.B.); (V.S.); (A.M.); (N.D.L.); (G.G.); (G.P.); (C.G.I.); (B.R.); (A.S.); (N.Q.); (M.J.); (G.D.); (G.F.)
Alberto Corriero Unit of Anesthesia and Resuscitation, Department of Emergencies and Organ Transplantations, Aldo Moro University, 70121 Bari, Italy; (A.C.); (M.R.); (N.B.)
Luigi Vimercati Department of Interdisciplinary Medicine, Section of Dental Medicine, University of Bari “Aldo Moro”, 70124 Bari, Italy; (A.D.I.); (G.M.); (S.C.); (A.P.); (L.V.); (D.A.); (G.M.); (G.C.); (F.P.); (G.B.); (V.S.); (A.M.); (N.D.L.); (G.G.); (G.P.); (C.G.I.); (B.R.); (A.S.); (N.Q.); (M.J.); (G.D.); (G.F.)
Daniela Azzollini Department of Interdisciplinary Medicine, Section of Dental Medicine, University of Bari “Aldo Moro”, 70124 Bari, Italy; (A.D.I.); (G.M.); (S.C.); (A.P.); (L.V.); (D.A.); (G.M.); (G.C.); (F.P.); (G.B.); (V.S.); (A.M.); (N.D.L.); (G.G.); (G.P.); (C.G.I.); (B.R.); (A.S.); (N.Q.); (M.J.); (G.D.); (G.F.)
Grazia Marinelli Department of Interdisciplinary Medicine, Section of Dental Medicine, University of Bari “Aldo Moro”, 70124 Bari, Italy; (A.D.I.); (G.M.); (S.C.); (A.P.); (L.V.); (D.A.); (G.M.); (G.C.); (F.P.); (G.B.); (V.S.); (A.M.); (N.D.L.); (G.G.); (G.P.); (C.G.I.); (B.R.); (A.S.); (N.Q.); (M.J.); (G.D.); (G.F.)
Giovanni Coloccia Department of Interdisciplinary Medicine, Section of Dental Medicine, University of Bari “Aldo Moro”, 70124 Bari, Italy; (A.D.I.); (G.M.); (S.C.); (A.P.); (L.V.); (D.A.); (G.M.); (G.C.); (F.P.); (G.B.); (V.S.); (A.M.); (N.D.L.); (G.G.); (G.P.); (C.G.I.); (B.R.); (A.S.); (N.Q.); (M.J.); (G.D.); (G.F.)
Fabio Piras Department of Interdisciplinary Medicine, Section of Dental Medicine, University of Bari “Aldo Moro”, 70124 Bari, Italy; (A.D.I.); (G.M.); (S.C.); (A.P.); (L.V.); (D.A.); (G.M.); (G.C.); (F.P.); (G.B.); (V.S.); (A.M.); (N.D.L.); (G.G.); (G.P.); (C.G.I.); (B.R.); (A.S.); (N.Q.); (M.J.); (G.D.); (G.F.)
Giuseppe Barile Department of Interdisciplinary Medicine, Section of Dental Medicine, University of Bari “Aldo Moro”, 70124 Bari, Italy; (A.D.I.); (G.M.); (S.C.); (A.P.); (L.V.); (D.A.); (G.M.); (G.C.); (F.P.); (G.B.); (V.S.); (A.M.); (N.D.L.); (G.G.); (G.P.); (C.G.I.); (B.R.); (A.S.); (N.Q.); (M.J.); (G.D.); (G.F.)
Vito Settanni Department of Interdisciplinary Medicine, Section of Dental Medicine, University of Bari “Aldo Moro”, 70124 Bari, Italy; (A.D.I.); (G.M.); (S.C.); (A.P.); (L.V.); (D.A.); (G.M.); (G.C.); (F.P.); (G.B.); (V.S.); (A.M.); (N.D.L.); (G.G.); (G.P.); (C.G.I.); (B.R.); (A.S.); (N.Q.); (M.J.); (G.D.); (G.F.)
Antonio Mancini Department of Interdisciplinary Medicine, Section of Dental Medicine, University of Bari “Aldo Moro”, 70124 Bari, Italy; (A.D.I.); (G.M.); (S.C.); (A.P.); (L.V.); (D.A.); (G.M.); (G.C.); (F.P.); (G.B.); (V.S.); (A.M.); (N.D.L.); (G.G.); (G.P.); (C.G.I.); (B.R.); (A.S.); (N.Q.); (M.J.); (G.D.); (G.F.)
Nicole De Leonardis Department of Interdisciplinary Medicine, Section of Dental Medicine, University of Bari “Aldo Moro”, 70124 Bari, Italy; (A.D.I.); (G.M.); (S.C.); (A.P.); (L.V.); (D.A.); (G.M.); (G.C.); (F.P.); (G.B.); (V.S.); (A.M.); (N.D.L.); (G.G.); (G.P.); (C.G.I.); (B.R.); (A.S.); (N.Q.); (M.J.); (G.D.); (G.F.)
Grazia Garofoli Department of Interdisciplinary Medicine, Section of Dental Medicine, University of Bari “Aldo Moro”, 70124 Bari, Italy; (A.D.I.); (G.M.); (S.C.); (A.P.); (L.V.); (D.A.); (G.M.); (G.C.); (F.P.); (G.B.); (V.S.); (A.M.); (N.D.L.); (G.G.); (G.P.); (C.G.I.); (B.R.); (A.S.); (N.Q.); (M.J.); (G.D.); (G.F.)
Giulia Palmieri Department of Interdisciplinary Medicine, Section of Dental Medicine, University of Bari “Aldo Moro”, 70124 Bari, Italy; (A.D.I.); (G.M.); (S.C.); (A.P.); (L.V.); (D.A.); (G.M.); (G.C.); (F.P.); (G.B.); (V.S.); (A.M.); (N.D.L.); (G.G.); (G.P.); (C.G.I.); (B.R.); (A.S.); (N.Q.); (M.J.); (G.D.); (G.F.)
Ciro Gargiulo Isacco Department of Interdisciplinary Medicine, Section of Dental Medicine, University of Bari “Aldo Moro”, 70124 Bari, Italy; (A.D.I.); (G.M.); (S.C.); (A.P.); (L.V.); (D.A.); (G.M.); (G.C.); (F.P.); (G.B.); (V.S.); (A.M.); (N.D.L.); (G.G.); (G.P.); (C.G.I.); (B.R.); (A.S.); (N.Q.); (M.J.); (G.D.); (G.F.)
Biagio Rapone Department of Interdisciplinary Medicine, Section of Dental Medicine, University of Bari “Aldo Moro”, 70124 Bari, Italy; (A.D.I.); (G.M.); (S.C.); (A.P.); (L.V.); (D.A.); (G.M.); (G.C.); (F.P.); (G.B.); (V.S.); (A.M.); (N.D.L.); (G.G.); (G.P.); (C.G.I.); (B.R.); (A.S.); (N.Q.); (M.J.); (G.D.); (G.F.)
Arnaldo Scardapane Department of Interdisciplinary Medicine, Section of Dental Medicine, University of Bari “Aldo Moro”, 70124 Bari, Italy; (A.D.I.); (G.M.); (S.C.); (A.P.); (L.V.); (D.A.); (G.M.); (G.C.); (F.P.); (G.B.); (V.S.); (A.M.); (N.D.L.); (G.G.); (G.P.); (C.G.I.); (B.R.); (A.S.); (N.Q.); (M.J.); (G.D.); (G.F.)
Luigi Curatoli Department Neurosciences & Sensory Organs & Musculoskeletal System, University of Bari “Aldo Moro”, 70124 Bari, Italy;
Nicola Quaranta Department of Interdisciplinary Medicine, Section of Dental Medicine, University of Bari “Aldo Moro”, 70124 Bari, Italy; (A.D.I.); (G.M.); (S.C.); (A.P.); (L.V.); (D.A.); (G.M.); (G.C.); (F.P.); (G.B.); (V.S.); (A.M.); (N.D.L.); (G.G.); (G.P.); (C.G.I.); (B.R.); (A.S.); (N.Q.); (M.J.); (G.D.); (G.F.) Department Neurosciences & Sensory Organs & Musculoskeletal System, University of Bari “Aldo Moro”, 70124 Bari, Italy;
Mario Ribezzi Unit of Anesthesia and Resuscitation, Department of Emergencies and Organ Transplantations, Aldo Moro University, 70121 Bari, Italy; (A.C.); (M.R.); (N.B.)
Maria Massaro Azienda Ospedaliero-Universitaria Consorziale Policlinico di Bari, 70124 Bari, Italy;
Megan Jones Department of Interdisciplinary Medicine, Section of Dental Medicine, University of Bari “Aldo Moro”, 70124 Bari, Italy; (A.D.I.); (G.M.); (S.C.); (A.P.); (L.V.); (D.A.); (G.M.); (G.C.); (F.P.); (G.B.); (V.S.); (A.M.); (N.D.L.); (G.G.); (G.P.); (C.G.I.); (B.R.); (A.S.); (N.Q.); (M.J.); (G.D.); (G.F.)
Ioana Roxana Bordea Department of Oral Rehabilitation, Faculty of Dentistry, Iuliu Hațieganu University of Medicine and Pharmacy, 400012 Cluj-Napoca, Romania;
Gianluca Martino Tartaglia UOC Maxillo-Facial Surgery and Dentistry, Department of Biomedical, Surgical and Dental Sciences, School of Dentistry, Fondazione IRCCS Ca Granda, Ospedale Maggiore Policlinico, University of Milan, 20100 Milan, Italy;
Antonio Scarano Department of Innovative Technologies in Medicine and Dentistry, University of Chieti-Pescara, 66100 Chieti, Italy;
Felice Lorusso Department of Innovative Technologies in Medicine and Dentistry, University of Chieti-Pescara, 66100 Chieti, Italy;
Luigi Macchia Department of Emergency and Organ Transplantation (D.E.T.O.), University of Bari Aldo Moro, 70124 Bari, Italy;
Angela Maria Vittoria Larocca Hygiene Complex Operating Unit, Azienda Ospedaliero-Universitaria Consorziale Policlinico di Bari, Place Giulio Cesare 11 BARI CAP, 70124 Bari, Italy;
Sergey Khachatur Aityan Director Multidisciplinary Research Center, Lincoln University, Oakland, CA 94612, USA;
Silvio Tafuri Department of Biomedical Science and Human Oncology, University of Bari, 70121 Bari, Italy;
Pasquale Stefanizzi Interdisciplinary Department of Medicine, University Hospital of Bari, 70100 Bari, Italy; (P.S.); (G.M.)
Giovanni Migliore Interdisciplinary Department of Medicine, University Hospital of Bari, 70100 Bari, Italy; (P.S.); (G.M.)
Nicola Brienza Unit of Anesthesia and Resuscitation, Department of Emergencies and Organ Transplantations, Aldo Moro University, 70121 Bari, Italy; (A.C.); (M.R.); (N.B.)
Gianna Dipalma Department of Interdisciplinary Medicine, Section of Dental Medicine, University of Bari “Aldo Moro”, 70124 Bari, Italy; (A.D.I.); (G.M.); (S.C.); (A.P.); (L.V.); (D.A.); (G.M.); (G.C.); (F.P.); (G.B.); (V.S.); (A.M.); (N.D.L.); (G.G.); (G.P.); (C.G.I.); (B.R.); (A.S.); (N.Q.); (M.J.); (G.D.); (G.F.)
Gianfranco Favia Department of Interdisciplinary Medicine, Section of Dental Medicine, University of Bari “Aldo Moro”, 70124 Bari, Italy; (A.D.I.); (G.M.); (S.C.); (A.P.); (L.V.); (D.A.); (G.M.); (G.C.); (F.P.); (G.B.); (V.S.); (A.M.); (N.D.L.); (G.G.); (G.P.); (C.G.I.); (B.R.); (A.S.); (N.Q.); (M.J.); (G.D.); (G.F.)
Francesco Inchingolo Department of Interdisciplinary Medicine, Section of Dental Medicine, University of Bari “Aldo Moro”, 70124 Bari, Italy; (A.D.I.); (G.M.); (S.C.); (A.P.); (L.V.); (D.A.); (G.M.); (G.C.); (F.P.); (G.B.); (V.S.); (A.M.); (N.D.L.); (G.G.); (G.P.); (C.G.I.); (B.R.); (A.S.); (N.Q.); (M.J.); (G.D.); (G.F.)

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Drozdzik A. Covid-19 and SARS-CoV-2 infection in periodontology: A narrative review. J Periodontal Res 2022;57:933-941. [PMID: 35839286 PMCID: PMC9350118 DOI: 10.1111/jre.13034] [Citation(s) in RCA: 3] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 01/08/2022] [Revised: 06/09/2022] [Accepted: 07/04/2022] [Indexed: 11/30/2022]

Abstract

The present review examined the available evidence on possible involvement of gingival tissues in SARS‐CoV‐2 infection. Gingival tissue possess SARS‐CoV‐2 entry and transmission factors, among them ACE2 (angiotensin‐converting enzyme 2) and TMPRSS2 (transmembrane protease serine 2), which are the principal mediators of the virus cell invasion. Clinical observations reveal SARS‐CoV‐2 RNA in periodontal tissues and crevicular fluid, suggesting that the periodontium may act as an entry point for the virus and/or as a dissemination site. The preliminary observations prove infection potential of gingival crevicular fluid and shed epithelial cells from the periodontium. There are also findings on potential associations between periodontitis and Covid‐19 (coronavirus disease 2019). PubMed and Scopus databases were used to search for suitable keywords such as: SARS‐CoV‐2, Covid‐19, oral virus infection, gingival crevicular fluid, oral mucosa, periodontium, gingiva, ACE2, TMPRSS2, Furin, diagnosis, topical treatment, vaccine and the related words for relevant publications. Data extraction and quality valuation of articles were performed by the author. The review addressed seven major domains: periodontal structures as SARS‐CoV‐2 infection site, the periodontal changes under SARS‐CoV‐2 infection, potential associations between periodontitis and Covid‐19, periodontal oral care in Covid‐19, crevicular fluid as potential transmission factor and preventive measures. The search process in PubMed and Scopus was updated up to 31 March 2022. Finally 68 articles were retrieved for the final analysis, from the initial database searches. According to the inclusion criteria articles in English language without any date restriction were included. The included studies were mostly original articles, and published in 2020 and 2021 with the aim to describe Covid‐19 and SARS‐CoV‐2 infection in periodontology. As a conclusion it can be stated that gingival tissues may play a role in SARS‐CoV‐2 infection.

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Hao M, Wang D, Xia Q, Kan S, Chang L, Liu H, Yang Z, Liu W. Pathogenic Mechanism and Multi-omics Analysis of Oral Manifestations in COVID-19. Front Immunol 2022;13:879792. [PMID: 35860279 PMCID: PMC9290522 DOI: 10.3389/fimmu.2022.879792] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [MESH Headings] [Grants] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 02/21/2022] [Accepted: 06/10/2022] [Indexed: 11/16/2022] Open