Many countries face an unprecedented challenge in aging demographics. This has led to an exponential growth in research on aging, which, coupled to a massive financial influx of funding in the private and public sectors, has resulted in seminal insights into the underpinnings of this biological process. However, critical validation in humans has been hampered by the limited translatability of results obtained in model organisms, additionally confined by the need for extremely time-consuming clinical studies in the ostensible absence of robust biomarkers that would allow monitoring in shorter time frames. In the future, molecular parameters might hold great promise in this regard. In contrast, biomarkers centered on function, resilience, and frailty are available at the present time, with proven predictive value for morbidity and mortality. In this review, the current knowledge of molecular and physiological aspects of human aging, potential antiaging strategies, and the basis, evidence, and potential application of physiological biomarkers in human aging are discussed.

Cardiopulmonary bypass (CPB) is widely used in cardiac operations. However, it remains unclear whether a CPB circuit, which is mainly made of plastics, can release micronanoplastics (MNPs) into the bloodstream. We conducted a prospective observational study involving children undergoing congenital heart disease repair with CPB support. Blood samples were collected before and after CPB and analyzed using pyrolysis-gas chromatography/mass spectrometry (Py-GC/MS) and laser direct infrared spectroscopy (LDIR) in combination with scanning electron microscopy. A total of 22 patients were involved in this study. The Py-GC/MS analysis revealed a significant increase in total MNPs after CPB support (p < 0.0001). Notably, CPB support significantly increased the levels of polystyrene (p = 0.046), polyethylene (p = 0.038), polypropylene (p < 0.0001), polyvinyl chloride (p < 0.0001), and polyamide 6 (p = 0.027). CPB time was positively correlated to MNP exposure (r = 0.43, p = 0.047). Increases in MNP exposure were positively correlated to an increase in white blood cells (r = 0.52, p = 0.013) and neutrophils (r = 0.46, p = 0.029). The LDIR analysis found that the post-CPB count of MNPs was significantly higher than the pre-CPB count (p = 0.015). In conclusion, CPB support significantly increases exposure to MNP in children undergoing cardiac operations. Further investigations are warranted to clarify the long-term health risks of MNP exposure caused by CPB support.

Human health depends on planetary health, and yet healthcare provision can have unintended consequences for the health of the planet. Emissions from the healthcare sector include greenhouse gases, air pollution and plastic pollution, alongside chemical contamination. Chemical pollution resulting in endocrine disruption has been associated with plastics, which are a source of concerning additives such as phthalates, bisphenols, perfluoroalkyl and polyfluoroalkyl substances, and flame retardants (all routinely found in healthcare products). Many endocrine-disrupting chemicals are persistent and ubiquitous in the environment (including water and food sources), with potential secondary harms for human health, including disrupting reproductive, metabolic and thyroid function. Here we review evidence-based strategies for mitigating environmental effects of healthcare delivery. We focus on what endocrinologists can do, including reducing demand for healthcare services through better preventative health, focusing on high-value care and improving sustainability of medical equipment and pharmaceuticals through adopting circular economy principles (including reduce, reuse and, as a last resort, recycle). The specific issue of endocrine-disrupting chemicals might be mitigated through responsible disposal and processing, alongside advocating for the use of alternative materials and replacing additive chemicals with those that have lower toxicity profiles, as well as tighter regulations. We must work to urgently transition to sustainable models of care provision, minimizing negative effects on human and planetary health.

Diabetes has become a global health crisis, affecting over 800 million people, with serious complications such as vascular and neurological damage. While diabetes management has been extensively studied, the impact of environmental pollutants, particularly microplastics (PS), on diabetic health remains poorly understood. PS, defined as plastic particles smaller than 5 mm, are pervasive and can enter the body through inhalation or ingestion, posing potential risks. However, the effects of PS exposure, particularly in diabetes, have not been adequately explored. Most studies focus on high-concentration, long-term exposure, which does not reflect typical human exposure levels. This study investigates the effects of short-term PS exposure on diabetic SD rats, using histological, apoptotic, and omics techniques, including metabolomics, lipidomics, and 16S rDNA sequencing. Our results show that short-term PS exposure exacerbates lung and intestinal damage in diabetic rats, with significant alterations in the gut microbiome. We also observed correlations between differential metabolites and microbiota changes. These findings provide novel evidence that short-term PS exposure, at concentrations reflecting daily contact, worsens metabolic dysfunction and intestinal dysbiosis in diabetes. This study emphasizes the need to consider environmental pollutants in diabetes management and highlights potential strategies for prevention and therapy.

This systematic review aims to further current knowledge on the effects of microplastics from orthodontic clear aligners, identifying potential implications for human health and providing a basis for further research and development of alternative materials. A literature search to find all peer-reviewed citations relevant to the review topic was conducted in the following databases: PubMed, Scopus, Web of Science, and Cochrane Library on 31 December 2024. A manual search of grey literature was also performed. There were 62 citations retrieved by the search query, and 11 were selected for inclusion in the review. Four selected studies were in vitro, while seven were in vitro following intraoral material aging studies. Ten studies evaluated the surface morphology of the material after aging, among the mechanical characteristics assessed, while only one article evaluated the chemical characteristics and size of the microplastic particles released from the aligners after simulated in vitro use. Discussion: From the evaluation of the studies included in this review, it is possible to state that there is a gradual increase over time in the surface roughness of the material, and modifications occurred in the morphology and surface topography of the aligners. Furthermore, it emerged that dispersion of microplastics occurs during the use of different types of aligners, with microplastic particle sizes ranging from 5 to 20 μm The findings suggest that clear aligners may cause microplastic dispersion in saliva during therapy, and this could cause a problem for the general health of patients, due to the absorption or ingestion of these released molecules. Further research is needed to fully understand the extent of microplastics released from aligners and to find alternative materials that can reduce this occurrence.

Micro- and nanoplastic particles occur ubiquitously in the environment and have been detected in various organs in animals and humans. We studied, how micro- and nanoplastic influence phagocytosis and intracellular killing of live bacteria in human monocytes.

Cells of the human reporter cell line THP1-Blue™ NFκB were pre-treated with different concentrations of micro- and nanoplastic (diameter 1 μm and 100 nm) and then incubated with Escherichia coli DH5α. Phagocytosis and intracellular killing was studied using an antibiotic protection assay. The activation of the NFκB promoter was quantified by measuring the production of alkaline phosphatase. Cytokines were measured by enzyme immunoassay. Cell viability was determined by trypan blue staining and lactate dehydrogenase measurement. Electron microscopic images were taken to localize micro- and nanoplastic.

Micro- and nanoplastic particles were rapidly internalized by monocytes. They reduced phagocytosis of E. coli in a concentration- and time-dependent manner. Exposure to micro- and nanoplastic also reduced the intracellular killing of bacteria in a concentration-dependent manner. Plain plastic particles did not induce NFκB synthesis and IL1β and IL6 release. At concentrations inhibiting phagocytosis, micro- and nanoplastic was not cytotoxic. Endotoxin stimulated phagocytosis of bacteria. High concentrations of plastic particles reduced the stimulatory effect of endotoxin on phagocytosis of bacteria, but not the effect on NFκB synthesis.

Exposure to micro- and nanoplastic reduced the ability of phagocytes to internalize and kill bacteria. High plastic concentrations decreased the endotoxin-stimulated phagocytosis of bacteria. Hence, exposure to plastic particles may reduce the host`s immune defence against bacterial pathogens.

Modern oral healthcare extensively uses polymer items and devices derived from various monomeric compounds. These materials are essential for personal protective equipment, infection barriers, packaging, and intraoral devices. The COVID-19 pandemic has led to an increased reliance on single-use polymer items, causing supply chain disruptions and higher costs. This systematic review explores the extent of polymer waste and pollution generated in oral healthcare clinics.

A systematic review protocol was registered with PROSPERO and was formatted according to PRISMA guidelines and SWiM recommendations. Eligibility criteria included studies that provided quantified estimates of polymer waste or pollution in air or wastewater from oral healthcare clinics. Comprehensive electronic searches were conducted across several bibliometric databases, followed by data extraction and risk of bias assessments performed by two independent reviewers.

A total of thirty studies were included in the review. Sixteen papers reported on waste audits that detailed polymer waste data, while eight studies focused on pollution caused by polymer nano- and microparticles in clinical settings. Additionally, six experimental studies investigated potential leakage of monomeric eluates or polymer particles from landfill waste. There was significant variation in the amount of polymer waste generated per patient, ranging from 81 to 384 g per operatory room per day. On-site sampling revealed the presence of polymer nano- and microparticles in the clinic air, which was influenced by dental procedures and the equipment used.

This review highlights critical knowledge gaps about polymer waste and pollution in oral healthcare clinics. The variability of study designs limited the feasibility of meta-analysis. Current evidence indicates substantial polymer waste generation, particularly from single-use items, as well as potential environmental impacts from monomeric eluates and polymer microparticles. Future research should focus on sustainable polymer waste management solutions to reduce environmental pollution in oral healthcare settings.

The problem of microplastics (MPs) pollution has caused many health risks to residents of Chinese cities. In this study, nine kinds of MPs or microrubbers (MRs) from dust fall (DF) in Xi'an, a megacity in northwestern China, were measured by pyrolysis-gas chromatography-mass spectroscopy, namely, polyethylene, polypropylene, nylon 88, polybutylene, polytetrafluorethylene, polyisoprene, polyvinyl chloride, natural rubber, and synthesis rubber. Here, 51.20% of MPs were extracted from the original DF (samples denoted DF-O). After the subtracting procedure, MPs and their residual (DF-S samples) were divided into two parts. Our results indicated that the DF-O and MPs samples exhibited higher cytotoxicity, inflammatory, and oxidative stress levels than the DF-S samples did. The DF-O and MPs samples suppressed autophagy by decreasing expression levels of microtubule-associated protein light chain 3 (LC3B), p-phosphatidylinositol 3-kinase (p-PI3K), phosphorylated AKT protein (p-Akt), and p-mammalian target of rapamycin (p-mTOR) while increasing the level of p62. Meanwhile, DF-O and MPs samples induced apoptosis through increasing levels of Bax/Bcl-2 and Cleaved Caspase-3/Caspase-3 in Raw264.7 cells. These trends could be reversed through removing half of the MPs in DF-O. Therefore, dust fall microplastics inhibited autophagy and induced apoptosis via activating the PI3K/Akt/mTOR pathway, increasing the Bax/Bcl-2 and Cleaved Caspase-3/Caspase-3 ratios. Here we provide a comprehensive perspective into the studies of atmospheric MPs pollution status and mechanisms of inhalation toxicity for health risk assessment of MPs in DF.

Environmental factors play a crucial role in modulating vascular inflammation, contributing significantly to the development of atherosclerosis and cardiovascular disease. This review synthesizes current evidence on how various environmental exposures influence vascular function and inflammation, with a focus on pollutants such as particulate matter and chemical toxins like bisphenols and per- and polyfluoroalkyl substances. These environmental stressors can trigger oxidative stress, chronic inflammation and vascular dysfunction, potentially accelerating the progression of atherosclerosis. We also explore the protective effects of natural compounds and exposure to green spaces in dampening inflammation and reducing cardiovascular risk. By examining the complex interplay between traditional risk factors and environmental exposures, this work highlights the need for comprehensive public health strategies that address both individual lifestyle factors and broader environmental determinants of cardiovascular health. We underscore the importance of further research to elucidate the precise cellular and molecular mechanisms by which environmental factors influence vascular function, with the aim of developing targeted interventions to mitigate their harmful effects and promote cardiovascular well-being.

Chemical recycling methods for post-consumer textile waste are effective for sustainable textile waste management. However, recycling synthetic and blended (cotton and synthetic) textiles can contribute to the release of microplastic fibers (MPFs) into the environment. This study investigated MPF release across different stages of two chemical recycling approaches, acid and alkaline hydrolysis, of polyester/cotton-blended textiles. Recycling involves various stages, including dye removal, treatment stage, and product. In the treatment stage, acid hydrolysis breaks down cotton into cellulose, leaving the polyester (PET) intact, whereas alkaline hydrolysis degrades PET, allowing cotton recovery. Across all stages, dye removal generated the highest MPF count, averaging nearly 10,055 MPFs g-1 of textile waste. Statistical analysis confirmed that the recycling approach significantly affected MPF release (p < 0.05), whereas the fabric type did not (p > 0.05). Alkaline hydrolysis reduced MPF release during the treatment stage by 87.55% compared to acid hydrolysis, indicating that recovering cotton and chemically degrading PET can significantly minimize MPF emissions during recycling. Ridge regression analysis identified the reaction conditions as key factors in MPF fragmentation, with blend ratios influencing the number of released MPFs. Surface characterization revealed treatment-induced fiber alterations, raising concerns regarding MPF emissions throughout the process. These findings highlight the textile recycling industries can be a source of MPF release into the environment, but recovering PET through degradation or dissolution can help minimize this impact of the treatment stage.

Humans are widely exposed to synthetic chemicals, especially via food. The types of chemical contaminants in food (including food contact chemicals) are diverse, and many of these are known to be hazardous, with mounting evidence that some contribute to noncommunicable diseases. The increasing consumption of ultra-processed foods, which contain synthetic chemicals, also contributes to adverse health. If the chemical contamination of foods were better characterized, then this issue would likely receive more attention as an important opportunity for disease prevention. In this Review, we discuss types and sources of synthetic food contaminants, focusing on food contact chemicals and their presence in ultra-processed foods. We outline future research needs and highlight possible responses at different food system levels. A sustainable transition of the food system must address the health impacts of synthetic chemicals in food; we discuss existing solutions that do justice to the complexity of the issue while avoiding regrettable substitutions and rebound effects.

In the contemporary era, named plasticene, the extensive presence of micro- and nanoplastics (MPs/NPs) in all environmental matrices constitutes a global challenge that impacts on living beings, including humans. Regardless of the route of exposure, the internalized MPs/NPs may reach the central nervous system and cause cytotoxicity. The effects of nano- and micro- polystyrene particles (n/mPS; 100 μg/mL), both in virgin (v) and home oxidized (ox) form, were assessed on the human neuroblastoma cells SH-SY5Y, treated for 24 h, using toxicological endpoints and 1H NMR-based metabolomics. A pro-oxidant effect was shown by reactive oxygen species (ROS) overproduction, present in virgin and oxidized particles, albeit 27.6 % and 29.5 % higher in ox-nPS and ox-mPS. DNA damage, mitochondrial impairment, and lipid peroxidation were found to be directly related to particle size and oxidation state (v-nPS < ox-nPS < v-mPS < ox-mPS). The metabolic changes induced by v- and ox- n/mPS in neuroblastoma cells involved the amino acid and energy metabolism, osmoregulation, oxidative stress, and neurotransmission. Interestingly, it was highlighted the ability of SH-SY5Y cells exposed to ox-nPS to counteract more effectively oxidative damage by reshaping metabolic pathways. Overall, the combination of toxicological assays and metabolic profiling confirmed the harmful effects induced by n/mPS to SH-SY5Y cells, always enhanced by the in home-oxidized counterpart, that led to cytotoxic effects and changes in cell metabolism. Despite a variable capacity for cellular homeostasis, the results shed light on the potential risks that these ubiquitous xenobiotics pose to human health, acting also as "triggers" for neurodegenerative diseases.

Obesity, diabetes, and their cardiovascular and hepatic comorbidities are alarming public health issues of the twenty-first century, which share mitochondrial dysfunction, oxidative stress, and chronic inflammation as common pathophysiological mechanisms. An increasing body of evidence links the combined exposure to multiple environmental toxicants with the occurrence and severity of metabolic diseases. Endocrine disruptors (EDs) are ubiquitous chemicals or mixtures with persistent deleterious effects on the living organisms beyond the endocrine system impairment; in particular, those known as metabolism-disrupting chemicals (MDCs), increase the risk of the metabolic pathologies in adult organism or its progeny. Being largely lipophilic, MDCs mainly target the adipose tissue and elicit mitochondrial dysfunction by interfering with mitochondrial bioenergetics, biogenesis, dynamics and/or other functions. Plastics, when broken down into micro- and nano-plastics (MNPs), have been detected in several human tissues, including the liver. The harmful interplay between inflammatory and redox processes, which mutually interact in a positive feed-back loop, hence the term oxidative inflammation ("OxInflammation"), occurs both at systemic and organ level. In both liver and adipose tissue, oxinflammation contributes to the progression of the metabolic dysfunction-associated steatotic liver disease (MASLD). Moreover, it has been reported that individuals with MASLD may be more susceptible to the harmful effects of toxicants (mainly, those related to mitochondria) and that chronic exposure to EDs/MDCs or MNPs may play a role in the development of the disease. While liver has been systematically investigated as major target organ for ambient chemicals, surprisingly, less information is available in the literature with respect to the adipose tissue. In this narrative review, we delve into the current literature on the most studied environmental toxicants (bisphenols, polychlorinated biphenyls, phthalates, tolylfluanid and tributyltin, per-fluoroalkyl and polyfluoroalkyl substances, heavy metals and MNPs), summarize their deleterious effects on adipose tissue, and address the role of dysregulated mitochondria and oxinflammation, particularly in the setting of MASLD.

Nano-microplastics (NMPs), as environmental pollutants, are widely present in nature and pose potential threats to biological health. This study aims to investigate the mechanisms by which NMPs inhibit mitophagy through the suppression of dickkopf-related protein 3 (DKK3) expression, leading to NOD-like receptor family, pyrin domain containing 3 (NLRP3) inflammasome-mediated cardiomyocyte pyroptosis and promoting myocardial fibrosis. Healthy adult male C57BL/6 mice were administered NMP solution via gavage, and their cardiac function was monitored. The results showed that NMP exposure significantly reduced left ventricular ejection fraction (LVEF) and left ventricular fractional shortening (LVFS) and increased the extent of myocardial fibrosis. Transcriptome sequencing identified 14 differentially expressed genes (DEGs), including MYL7. Using the random forest algorithm and functional enrichment analysis, DKK3 was identified as a key gene. In Vitro experiments further confirmed that NMPs downregulate DKK3 expression, thereby inhibiting mitophagy and promoting cardiomyocyte pyroptosis. This study elucidates the molecular mechanisms by which NMPs induce myocardial fibrosis and provides new theoretical bases and molecular targets for the diagnosis and treatment of heart diseases.

Recent studies and OECD (Organization for Economic Cooperation and Development) reports provide roadmaps to reduce dispersal of mismanaged plastic waste to aquatic environments. Here, we use a coupled land-ocean-atmosphere model to simulate global plastic and microplastic dispersal for different OECD policy scenarios toward 2060. We establish a global plastic budget for the year 2015, with revised estimates of the total marine plastic pool of 263 teragrams (Tg, million tons), and land to sea plastic transport of 14 Tg per year, implying four to nine times larger leakage than OECD estimates. Model simulation of two ambitious policy scenarios show a peak in land to sea transport of total plastics of 23 Tg per year around 2045 and a decrease thereafter. Environmental concentrations of small microplastics remain high after 2060 due to continuous fragmentation of legacy mismanaged waste on land and indicate the need for remediation of legacy terrestrial plastic waste in policy instruments.

Climate change is like cancer, delayed action leads to more suffering for patients.

Intersectionality describes interdependent systems of inequality related to sex, gender, race, age, class and other socio-political dimensions. By focusing on the compounded effects of social categories, intersectional analysis can enhance the accuracy and experimental efficiency of science. Here we extend intersectional approaches that were predominantly developed in the humanities, social sciences and public health to the fields of natural science and technology, where this type of analysis is less established. Informed by diverse global and disciplinary examples-from enhancing facial recognition for diverse user bases to mitigating the disproportionate impact of climate change on marginalized populations-we extract methods to demonstrate how quantitative intersectional analysis functions throughout the research process, from strategic considerations for establishing research priorities to formulating research questions, collecting and analysing data and interpreting results. Our goal is to offer a set of guidelines for researchers, peer-reviewed journals and funding agencies that facilitate systematic integration of intersectional analysis into relevant domains of science and technology. Precision in research best guides effective social and environmental policy aimed at achieving global equity and sustainability.

Cardiovascular disease is the leading cause of morbidity and mortality worldwide, with a substantial amount of health-care resources targeted towards its diagnosis and management. Environmental sustainability in cardiovascular care can have an important role in reducing greenhouse gas emissions and pollution and could be beneficial for improving health metrics and societal well-being and minimizing the cost of health care. In this Review, we discuss the motivations and frameworks for sustainable cardiovascular care with an emphasis on the reduction of the climate-related and environmental effects of cardiovascular care. We also provide an overview of greenhouse gas emissions related to the provision of health care, including their measurement and quantification, carbon accounting, carbon disclosures and climate effects. The principles of life-cycle assessment, waste prevention and circular economics in health care are discussed, and the emissions associated with various sectors of cardiovascular care as well as the rationale for prevention as a powerful approach to reduce these emissions are presented. Finally, we highlight the challenges in environmental sustainability and future directions as applicable to cardiovascular practice.

Rising global concentrations of environmental microplastics and nanoplastics (MNPs) drive concerns for human exposure and health outcomes. Complementary methods for the robust detection of tissue MNPs, including pyrolysis gas chromatography-mass spectrometry, attenuated total reflectance-Fourier transform infrared spectroscopy and electron microscopy with energy-dispersive spectroscopy, confirm the presence of MNPs in human kidney, liver and brain. MNPs in these organs primarily consist of polyethylene, with lesser but significant concentrations of other polymers. Brain tissues harbor higher proportions of polyethylene compared to the composition of the plastics in liver or kidney, and electron microscopy verified the nature of the isolated brain MNPs, which present largely as nanoscale shard-like fragments. Plastic concentrations in these decedent tissues were not influenced by age, sex, race/ethnicity or cause of death; the time of death (2016 versus 2024) was a significant factor, with increasing MNP concentrations over time in both liver and brain samples (P = 0.01). Finally, even greater accumulation of MNPs was observed in a cohort of decedent brains with documented dementia diagnosis, with notable deposition in cerebrovascular walls and immune cells. These results highlight a critical need to better understand the routes of exposure, uptake and clearance pathways and potential health consequences of plastics in human tissues, particularly in the brain.

As an emerging environmental pollutant, microplastics have attracted increasing attention to their potential health hazards. However, the current understanding about the toxicity and health implications, especially about developmental toxicity with exposure to microplastics is quite limited. In the current study, we aimed to scrutinize the deleterious effects of polystyrene microplastics (PSMPs) with different sizes (0.1 and 5 μm) on the placenta that plays crucial role in fetal development, following oral exposure during gestational stages. The results showed that two sizes of PSMPs could distribute in mouse placental tissues, and nanosized PSMPs (0.1 μm) exhibited greater capability to penetrate the placenta and deposit in the liver and brain of fetuses than microsized PSMPs (5 μm). Importantly, only 0.1 μm PSMPs induced a decrease in the junctional area, a reduction in the labyrinthine vascularization and an increase in cell apoptosis in the placenta, accompanied by fetal developmental impairments. The results of metabolome and transcriptome uncovered that 0.1 μm PSMP exposure caused changes in metabolic and gene profiles of placental tissues, across multiple pathways such as vascular supply, nutrient absorption and transportation and amino acid metabolism. Overall, our results confirmed that maternal PSMP exposure led to placental damages associated with metabolic and gene expression disorders. This study would provide new insights into the developmental impacts of microplastic consumption during gestation.

Elderly individuals diagnosed with neuroblastoma and peripheral nervous system tumours often have a poor prognosis. However, there is currently a lack of comprehensive analysis on these conditions in older adults. This study aims to determine the global epidemiological trends of neuroblastoma and peripheral nervous system tumours (in individuals aged 60 and above).

We obtained cross-sectional data from the 2021 Global Burden of Disease, Injuries, and Risk Factors Study (GBD) ( https://vizhub.healthdata.org/gbd-results/ ). We assessed the burden of neuroblastoma and peripheral nervous system tumours in the elderly from 1990 to 2021 using indicators such as prevalence and incidence. These indicators were classified by global, national, and regional levels, further stratified by Socio-Demographic Index (SDI), age, and gender. The results are organized by SDI, age, and gender categories.

From 1990 to 2021, the global age-standardised prevalence and incidence rates of neuroblastoma and peripheral nervous system tumours among the elderly increased from 0.06 (95% UI 0.05, 0.08) and 0.12 (95% UI 0.09, 0.15) per 100,000 to 0.11 (95% UI 0.09, 0.13) and 0.22 (95% UI 0.17, 0.26) per 100,000, respectively. Age-standardised mortality and DALY rates also rose. Central Europe had the highest age-standardised prevalence and incidence rates in 2021, while Eastern Europe had the highest DALY rate. East Asia reported the highest number of total cases and experienced the fastest growth, with significant increases in prevalence, incidence, mortality, and DALY rates. Gender disparities were evident, with elderly men showing higher rates than women, and greater EAPC values indicating a higher increase in disease burden over time. The highest age-specific rates were found in the 90-94 age group, while the 70-74 age group had the highest DALY burden.

The continuous rise in the incidence of neuroblastoma and peripheral nervous system tumours among the elderly highlights a pressing the necessity for focused public health measures and improved treatment approaches. Addressing the regional, gender, and age-related disparities requires a comprehensive approach that integrates medical advancements, social support, and public health policies. Future research should explore potential risk factors and innovative therapies to mitigate this growing global health challenge.

The leakage and accumulation of plastic in the environment is a significant and growing problem with numerous detrimental impacts and has led to a push toward the design and development of more environmentally benign materials. To this end, we have developed a quantum chemistry-based model for predicting the mobility of polymer materials from molecular structure. Hydrophobicity is used as a surrogate for mobility given that hydrophobic interactions drive much of the partitioning of contaminants in and out of various environmentally relevant compartments. To model polymer hydrophobicity, we adjusted a previously developed Quantum Chemically Calculated Abraham Parameter model to calculate Abraham parameters of small molecules from molecular structure information. The resulting model predicted the octanol-water partition coefficient (KOW) of polymer repeating units with a root mean square error (RMSE) of 0.48 (log scale). Additionally, the hydrophobicity of high molecular weight polymer materials was captured through solubility parameters and Nile red staining experiments from the literature and predicted with RMSEs of 1.21 (J/cc)0.5 and 3.42 nm, respectively. Finally, to test the environmental applicability of the model, the relative adsorption capacity of three polymers was predicted and used to unify sorption isotherms across multiple sorbates and polymer sorbents.

To assess the impact of microplastics/nanoplastics (MiP/NP) on human health.

The authors conducted a narrative review of articles published in English, Portuguese, French and Spanish in the last decade in the following databases: PubMed, Google Scholar, EMBASE and SciELO. The keywords used in this search were: microplastics OR nanoplastics OR marine litter OR toxicology OR additives AND human health OR children OR adults.

MiP is a group of emerging contaminants that have attracted increasing scientific interest and attention from society in the last decade due to their ubiquitous detection in all environments. Humans can be mainly exposed to MiP and NP orally, by inhalation, by dermal contact, as well as through systemic routes and cannot be neglected, especially in young children. The possible toxic effects in different systems are due to plastic particles, often combined with leachable additives and adsorbed contaminants.

Unless the plastics value chain is transformed in the next two decades, the risks to species, marine ecosystems, climate, health, economies and communities will become unmanageable. However, alongside these risks lie unique opportunities to lead the transition to a more sustainable world.

Background: Microplastics (MPs) are small plastic fragments with diameters less than 5 mm in size and are prevalent in everyday essentials and consumables. Large global plastic production has now led to a flooding of MPs in our natural environment. Due to their detrimental impacts on the planet's ecosystems and potentially our health, MPs have emerged as a significant public health concern. In this pilot study, we hypothesize that MPs exposure will negatively affect gut microbiota composition and function, in which metabolic reprogramming plays an important role. Methods: Using in vitro experiments, three bacterial strains (Escherichia coli MG1655, Nissle 1917, and Lactobacillus rhamnosus) were selected to investigate the impacts of MPs exposure. The bacterial strains were individually cultured in an anaerobic chamber and exposed to 1 µm polystyrene MPs at various concentrations (0, 10, 20, 50, 100, and 500 µg/mL) in the culture medium. Results: MPs exposure reduced the growth of all three bacterial strains in a dose-dependent manner. Liquid chromatography mass spectrometry (LC-MS)-based untargeted metabolomics revealed significant differences in multiple metabolic pathways, such as sulfur metabolism and amino sugar and nucleotide sugar metabolism. In addition, we extracted gut microbiota from C57BL/6 mice, and 16S rRNA sequencing results showed a significant upregulation of Lactobacillales and a significant reduction in Erysipelotrichales due to MPs exposure. Furthermore, targeted and untargeted metabolomics corroborated the in vitro results and revealed alterations in microbial tryptophan metabolism and energy producing pathways, such as glycolysis/gluconeogenesis and the pentose phosphate pathway. Conclusions: These findings provide evidence that MPs exposure causes comprehensive changes to healthy gut microbiota, which may also provide insights into the mechanistic effects of MPs exposure in humans.

The Eastern Mediterranean Sea, rich in environmental and cultural heritage, faces increasing threats from emerging contaminants like toxic metals and phthalates. This study evaluates their occurrence across 40 Lebanese Mediterranean coastal hotspots using advanced techniques such as Inductively Coupled Plasma Mass Spectrometry (ICP-MS), X-ray Fluorescence Spectroscopy (XRF) and Gas Chromatography-Mass Spectrometry (GC-MS). Widespread contamination was detected in both water and sediments near landfills, sewage discharges, and industrial areas. Metal concentrations varied, with chromium (Cr) reaching 20.3 µg/L, arsenic (As) 12.1 µg/L, and lead (Pb) 30.6 µg/L, indicating pollution from urban and industrial activities. Manganese (Mn) and zinc (Zn) were within safe limits, while selenium (Se) and arsenic posed ecological risks. Among 13 phthalates, diethylhexyl phthalate (DEHP) was the most prevalent, ranging from 15.57 to 72.88 µg/L. Sediments showed elevated calcium, strontium, and barium levels, exceeding safety thresholds. Statistical analysis revealed correlations between contaminants and spatial variability driven by industrial, agricultural, and urban activities. These findings highlight the need for proper regulations and routine monitoring to protect marine ecosystems and public health.

Concerns persist about the potential impact of prenatal exposure to bisphenols (BP) and their replacement analogues on childhood asthma and allergies. Previous studies on single and small cohorts had limited statistical power, few investigated analogues BPF and BPS, and even fewer examined atopic outcomes. Our objective was to assess whether prenatal exposures to individual environmental bisphenols (BPA, BPF, BPS) influence risk of childhood asthma, allergic rhinitis, and atopic dermatitis. Data from the U.S. Environmental Influences on Child Health Outcomes (ECHO) consortium were harmonized on measures of prenatal urinary BPA, BPF and BPS and asthma and allergic rhinitis (ages 5-9 years) and atopic dermatitis (up to age 3 years) from 1905 mother-child pairs that were collected between 1998 and 2017. Across the 2012 federal ban of BPA from certain infant products, median BPA levels decreased from 1.11 ng/ml to 0.86 ng/ml; median BPF levels decreased from 0.51 ng/ml to 0.39 ng/ml; and median BPS levels increased from 0.23 ng/ml to 0.31 ng/ml (dilution adjusted; p < 0.001 for all three median comparisons). Prenatal measures of BPA, BPF, and BPS were unrelated to the risk of childhood asthma, allergic rhinitis, or atopic dermatitis in the total population. Modest sex-dependent effects were observed: only among girls, second tertile levels of BPF was associated with a reduced odds of asthma (odds ratio (OR) 0.27, 95% confidence interval (CI) 0.08, 0.93); a continuous index of prenatal BPS was associated with reduced odds of atopic dermatitis (OR 0.64, 95% CI 0.44, 0.93). The ongoing and changing patterns of exposure to bisphenols in the U.S. population require further study with additional attention to time windows of exposure and co-occurring social determinants of health, to continue to inform current policies and evaluate the importance of limiting exposure to BPA and its analogues on childhood asthma, allergic rhinitis, and atopic dermatitis.

The male reproductive system has been the subject of considerable attention in recent years due to the adverse effects of Di (2-ethylhexyl) phthalate (DEHP). Although previous research has suggested that DEHP exposure hinders the early meiotic progression of male germ cells, the underlying mechanisms are still not well understood. The transcriptomic changes in testicular cells of postnatal male rodents following DEHP exposure were meticulously analyzed using 10X Genomics single-cell RNA sequencing in this study. For downstream analysis, we acquired 42,000 cells and generated 3172,754,990 reads. DEHP exposure at concentrations of 40 μg/kg/day (DEHP40) and 80 μg/kg/day (DEHP80) substantially decreased the proportion of pachytene and diplotene spermatocytes, indicating a shared inhibitory effect on early meiosis, as demonstrated by our findings. In addition, DEHP exposure disrupted the cellular communication between Sertoli cells and germ cells, which had a significant impact on the p38-MAPK signaling pathway. The expression of key ligand genes Tgfb1 and Tgfb3 in Sertoli cells was significantly reduced. DEHP exposure resulted in a substantial decrease in the expression of the Trp53 gene, which in turn down-regulated three critical downstream genes (Stmn1, Tubb5, and Ccnb1) that are implicated in spindle organization from a mechanistic perspective. This study offers the first comprehensive evidence that DEHP inhibits early meiotic progression in male germ cells through the Trp53-mediated p38-MAPK pathway, providing crucial insights into the molecular mechanisms underlying DEHP-induced male reproductive toxicity. Our results emphasize the enduring negative effects of DEHP exposure on male fertility, which have substantial ramifications for the comprehension and mitigation of the influence of environmental estrogens on reproductive health.

Individual attitudes and knowledge can predict pro-environmental behaviors. Public surveys, therefore, can provide precious information, which can guide sensitization interventions. In this systematic review, we searched Medline and Embase, with no language or date restrictions, for surveys designed to measure in the general population the level of knowledge about different types of plastics, the risks associated with plastic pollution, and awareness of actions to reduce them. Survey tools were analyzed following the guide of Burns and Kho, and study methodological quality was assessed via the Appraisal Tool for Cross-Sectional Studies. We included 17 articles published from 2019 to 2024, mostly concerning European populations. The tools comprised a median of 13 items (range 7-50), and very differently formulated questions. Overall, 13/17 (76.5%) study questionnaires received less than 50% (<3.5) of the maximum possible score. The remaining four questionnaires obtained intermediate scores (between 3.5 and 5.3) indicating moderate quality. Most studies did not employ the appropriate cross-sectional survey methodology, only two studies statistically justified sample sizes, only three reported a sampling frame, and only two described a selection process that appears to be representative. In most cases, the instruments were not validated, and the statistical significance of key variables was not provided. The many shortcomings highlighted in this review emphasize the urgent need for methodological rigor when conducting survey studies, which are essential tools for public health.

Hazards to human and planetary health are present at every stage of the plastic life cycle, yet plastic production is projected to triple by 2060. This review focuses on three key areas: the life cycle of plastic, the impact of microplastics and their associated chemicals, along with recommendations to reduce plastic use. In dermatology, micro- and nanoplastics are especially problematic as they are present in over 90% of personal care products. They have been detected in utero, absorbed through the skin and found in the lungs and gastrointestinal tract. Numerous in vitro and animal studies have substantiated the negative impact of micro- and nanoplastics but gaps remain with regards to their effect on human health. In spite of this limitation, we review the evidence to date and offer evidence-based recommendations which can reduce plastic production, enhance health outcomes and promote environmental justice.

In May 2021, the M/V X-Press Pearl ship fire disaster led to the largest maritime spill of resin pellets (nurdles) and burnt plastic (pyroplastic). Field samples collected from beaches in Sri Lanka nearest to the ship comprised nurdles and pieces of pyroplastic. Three years later, the toxicity of the spilled material remains unresolved. To begin understanding its potential toxicity, solvent extracts of the nurdles and pyroplastic were screened for their bioactivity by several Attagene FACTORIAL bioassays (TF, NR, and AquaTox), which measured the activity of a combined 70 human transcription factor response elements and nuclear receptors and 6 to 7 nuclear receptors for each of three phylogenetically distinct fish species. Extracts of the pyroplastics robustly activated end points for the human aryl hydrocarbon receptor (AhR), estrogen receptor (ER), pregnane X receptor (PXR), peroxisome proliferator-activated receptor (PPAR), retinoid X receptor (RXR), and oxidative stress (NRF2) and had the potential for activation of several others. The bioactivity profile of the pyroplastics was most similar (similarity score = 0.96) to that of probable human carcinogens benzo[b]fluoranthene and benzo[k]fluoranthene despite the extracts being a complex mixture of thousands of compounds. The activity diminished only slightly for extracts of pyroplastic collected eight months after the spill. The AquaTox FACTORIAL bioassay measured the activation of ERα, ERβ, androgen receptor (AR), PPARα, PPARγ, and RXRβ for human, zebrafish (Danio rerio), Japanese medaka (Oryzias latipes), and rainbow trout (Oncorhynchus mykiss), revealing species-specific sensitivities to the chemicals associated with the pyroplastics. These findings provide needed information to guide long-term monitoring efforts, make hazard assessments of the spilled material, and direct further research on pyroplastic, an emerging global contaminant.

The Mediterranean Sea is an intercontinental marine environment renowned for its biodiversity and ecological significance. However, it is also one of the most polluted seas globally with significant levels of microplastics and heavy metals among other emerging contaminants. In Lebanon, inadequate waste management infrastructure and unregulated industrial discharges have exacerbated water quality deterioration by introducing these complex contaminants into surface and seawater. The Palm Islands Natural Reserve in Lebanon is a UNESCO-designated marine protected area and home to endangered species. However, the reserve faces significant threats from pollution, including heavy metals and microplastics, exacerbated by nearby Tripoli's escalating contamination. Plasticisers, particularly phthalates, are recognized for their hormone-disrupting effects, and heavy metals like cadmium, lead, and arsenic pose severe eco-toxicological risks. This study investigates the levels of heavy metals and phthalates in water and sediments from the Palm Islands. Samples were collected from different locations within the reserve, and heavy metals and phthalates were detected, including chromium (13.58 to 19.28 μg L-1), arsenic (2.05 to 5.04 μg L-1), cadmium (1.27 to 3.04 μg L-1), and lead (0.92 to 2.88 μg L-1). Cadmium levels exceeded the permissible limits set by environmental regulatory bodies, highlighting an urgent pollution problem. Phthalates, including DEP and DEHP, were also detected in concentrations of 7.12-10.25 μg L-1 for DEP and 38.47-56.12 μg L-1 for DEHP raising concerns over their potential eco-toxicological impact on marine species. Our research underscores the need for comprehensive environmental monitoring, better waste management infrastructure, and stricter regulatory measures to address pollution in Lebanon's coastal ecosystems.

Objectives: The objective of this narrative literature review was to highlight all dental procedures attributable to sectoral waste and to consider possible alternatives in line with the concept of sustainable development. Methods: An extensive search of electronic databases, including the Cochrane Oral Health Group Specialized Register, Cochrane Central Register of Controlled Trials (CENTRAL), Web of Science, PubMed, EMBASE, and Google Scholar. Search words included 'Green Dentistry', 'Dental Pollution', 'Pollutants and Dentistry', 'Disinfectants and Dentistry', and 'High-tech Dentistry'. All of them allowed an assessment of the impact of dental practice on the external environment, and new frontiers currently applied or possibly applicable for green dentistry were included in the study. Non-full-text papers, animal studies, studies in languages other than English, and studies not related to the topic under consideration were excluded. Results: According to the inclusion criteria, 76 papers were selected for the study. The topics analyzed were the impact of dental practice on the outdoor environment, currently applied and potentially applicable principles of green dentistry, and the 'Four Rs' model (Rethink, Reduce, Reuse, and Recycle). Conclusions: With the limitations of the present study, the concept of green dentistry could be applicable provided that the measures already taken to reduce indoor and outdoor risk factors are continued and improved.

Microplastics (MP) contamination in food and water poses significant health risks. While microbes that form biofilm show potential for removing MP from the environment, no methods currently exist to eliminate these non-degradable MP from the human body. In this study, we propose using probiotics to adsorb and remove ingested MP within the gut. We conducted a comprehensive evaluation of 784 bacterial strains to assess their ability to adsorb 0.1 μm polystyrene particles using a high-throughput screening method. Among the tested strains, Lacticaseibacillus paracasei DT66 and Lactiplantibacillus plantarum DT88 exhibited optimal adsorption in vitro and were effective across various MP types. In an animal model, mice treated with these probiotics demonstrated a 34% increase in PS excretion rates and a 67% reduction in residual polystyrene (PS) particles within the intestine. Additionally, administration of Lactiplantibacillus plantarum DT88 mitigated PS-induced intestinal inflammation. Together, our findings demonstrate a novel probiotic strategy for addressing MP-associated health risks, emphasizing the potential of strain-specific probiotics to remove MP from the gut environment.

Epidemiological evidence from the past 20 years indicates that environmental chemicals brought into the air by the vaporization of volatile organic compounds and other anthropogenic pollutants might be involved, at least in part, in the development or progression of psychiatric disorders. This evidence comes primarily from occupational work studies in humans, with indoor occupations being the most important sources of airborne pollutants affecting neural circuits implicated in mood disorders (e.g., major depressive disorder and bipolar disorder). The current mini review brings together recent findings of indoor airborne pollution from different fields of research, including genetics, neuropathology, and neuroimaging, for gauging underlying physiological mechanisms leading to emotional disturbances that impact nearly all aspects of human behavior. A better understanding of how indoor airborne pollutants affect brain neurons to augment clinical symptoms associated with psychiatric disorders will undoubtedly be useful in the subsequent treatment of patients with major depressive and/or bipolar disorders. This article is part of the themed issue, "Understanding the Link Between Environmental Pollutants, Brain & Behavior."

Are bisphenols released from disposable devices used in assisted reproductive technology (ART) procedures, and do they accumulate when several disposable devices are used sequentially under routine conditions?

A comprehensive assessment of 19 individual disposable devices (31 assessments) and nine combinations of disposable devices replicating the main steps in an ART procedure was undertaken. The extraction of bisphenols followed routine-use conditions (temperature and duration). The concentrations of 10 bisphenols were determined using online solid-phase extraction/liquid chromatography/mass spectrometry methodology.

Bisphenol S (BPS) was quantified consistently from 100-mm culture dishes (32 ± 20 pg) and from high security sperm straws (3 ± 1 pg). Also, BPS and bisphenol A (BPA) were quantified consistently from spermicide-free condoms (95 ± 78 and 83 ± 49 pg, respectively). No other bisphenols were detected in disposable devices when tested individually. When disposable devices were used in combination, both BPA and BPS were detected consistently in combinations of 13 disposable devices mimicking sperm collection in a condom and its preparation (46 ± 16 and 43 ± 32 pg, respectively). BPS was quantified consistently in combinations of 14 disposable devices mimicking sperm collection, its preparation and freezing (10 ± 4 pg), and in combinations of 17 disposable devices mimicking oocyte retrieval (37 ± 22 pg).

BPA and BPS are released in small quantities from some disposable devices used in routine conditions during ART procedures, but do not appear to accumulate when these disposable devices are used in combination.

Commercial plastics are potentially hazardous and can be carcinogenic due to the incorporation of chemical additives along with other additional components utilized as brominated flame retardants and phthalate plasticizers during production that excessively produce large numbers of gases, litter, and toxic components resulting in environmental pollution.

Biodegradable plastic derived from natural renewable resources is the novel, alternative, and innovative approach considered to be potentially safe as a substitute for traditional synthetic plastic as they decompose easily without causing any harm to the ecosystem and natural habitat. The utilization of undervalued compounds, such as by-products of fruits and vegetables in the production of biodegradable packaging films, is currently a matter of interest because of their accessibility, affordability, ample supply, nontoxicity, physiochemical and nutritional properties. Industrial food waste was processed under controlled conditions with appropriate plasticizers to extract polymeric materials. Biodegradability, solubility, and air test analysis were performed to examine the physical properties of polymers prior to the characterization of the biofilm by Fourier-transformed infrared spectroscopy (FTIR) for the determination of polymeric characteristics.

The loss of mass examined in each bioplastic film was in the range of 0.01g to 0.20g. The dimension of each bioplastic was recorded in the range of 4.6 mm to 28.7 mm. The existence of -OH, C=C, C=O stretching, and other crucial functional groups that aid in the creation of a solid polymeric material are confirmed by FTIR analysis. This study provides an alternative approach for sustainable and commercially value-added production of polymeric-based biomaterials from agro-industrial waste as they are rich in starch, cellulose, and pectin for the development of bio-plastics.

The rationale of this project is to achieve a straightforward, economical, and durable method for the production of bio-plastics through effective utilization of industrial and commercial fruit waste, ultimately aiding in revenue generation.

This study aims to enhance prognostic accuracy in severe traumatic brain injury (STBI) by developing a novel nomogram that integrates clinical and paraclinical data.

Data from 263 STBI patients were analyzed, focusing on critical variables such as age, Glasgow Coma Scale scores, pupil responsiveness, CT findings, and blood markers. A rigorous regression analysis was conducted to identify significant predictors. The nomogram underwent internal and external validation, and its predictive performance was compared with existing models through a meta-analysis.

The novel nomogram demonstrated superior predictive accuracy for STBI outcomes compared to traditional models. Key predictors, including age, Glasgow Coma Scale scores, pupil responsiveness, CT findings, and specific blood markers, were harmonized to provide a more precise prognostic tool. Validation processes confirmed the robustness and reliability of the nomogram.

The developed nomogram represents a significant advancement in STBI prognosis, offering clinicians a powerful tool to improve patient care strategies. By integrating CT imaging and blood parameters, the nomogram enhances the precision of outcome predictions, facilitating better-informed clinical decisions.

More than 16,000 chemicals are incorporated into plastics to impart properties such as color, flexibility, and durability. These chemicals may leach from plastics, resulting in widespread human exposure during everyday use. Two plastic-associated chemicals-bisphenol A (BPA) and di(2-ethylhexyl) phthalate (DEHP)-and a class of chemicals-brominated flame retardants [polybrominated diphenyl ethers (PBDEs)]-are credibly linked to adverse health and cognitive impacts. BPA exposures are associated with ischemic heart disease (IHD) and stroke, DEHP exposure with increased all-cause mortality among persons 55 to 64 y old, and prenatal PBDE exposures in mothers with IQ losses in their children. We estimate BPA, DEHP, and PBDE exposures in 38 countries containing one-third of the world's population. We find that in 2015, 5.4 million cases of IHD and 346,000 cases of stroke were associated with BPA exposure; that DEHP exposures were linked to approximately 164,000 deaths among 55-to-64 y olds; and that 11.7 million IQ points were lost due to maternal PBDE exposure. We estimate the costs of these health impacts to be $1.5 trillion 2015 purchasing power parity dollars. If exposures to BPA and DEHP in the United States had been at 2015 levels since 2003, 515,000 fewer deaths would have been attributed to BPA and DEHP between 2003 and 2015. If PBDE levels in mothers had been at 2015 levels since 2005, over 42 million IQ points would have been saved between 2005 and 2015.

In this study, we have investigated microbial communities structure and function using high throughput amplicon sequencing and whole metagenomic sequencing of DNA extracted from different depths of a plastic-laden landfill site. With diverse taxonomic groups inhabiting the plastic-rich soil, our study demonstrates the remarkable adaptability of microbes to use this new substrate as a carbon source. FTIR spectroscopic analysis of soil indicated degradation of plastic as perceived from the carbonyl index of 0.16, 0.72, and 0.44 at 0.6, 0.9 and 1.2 m depth, respectively. Similarly, water contact angles of 108.7 degree, 99.7 degree, 62.7 degree, and 77.8 degree of plastic pieces collected at 0.3, 0.6, 0.9, and 1.2 m depths respectively showed increased wettability and hydrophilicity of the plastic. Amplicon analysis of 16S and 18 S rRNA revealed a high abundance of several plastic-degrading bacterial groups, including Pseudomonas, Rhizobiales, Micrococcaceae, Chaetomium, Methylocaldum, Micromonosporaceae, Rhodothermaceae and fungi, including Trichoderma, Aspergillus, Candida at 0.9 m. The co-existence of specific microbial groups at different depths of landfill site indicates importance of bacterial and fungal interactions for plastic. Whole metagenome analysis of soil sample at 0.9 m depth revealed a high abundance of genes encoding enzymes that participate in the biodegradation of PVC, polyethylene, PET, and polyurethane. Curation of the pathways related to the degradation of these materials provided a blueprint for plastic biodegradation in this ecosystem. Altogether, our study has highlighted the importance of microbial cooperation for the biodegradation of pollutants. Our metagenome-based investigation supports the current perception that consortia of fungi-bacteria are preferable to axenic cultures for effective bioremediation of the environment.