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Inchingolo F, Inchingolo AM, Latini G, de Ruvo E, Campanelli M, Palermo A, Fabbro MD, Blasio MD, Inchingolo AD, Dipalma G. Guided Bone Regeneration: CGF and PRF Combined With Various Types of Scaffolds-A Systematic Review. Int J Dent 2024; 2024:4990295. [PMID: 39669891 PMCID: PMC11637628 DOI: 10.1155/ijod/4990295] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 05/31/2024] [Accepted: 11/08/2024] [Indexed: 12/14/2024] Open
Abstract
Objective: Bone regeneration plays a pivotal role in modern oral surgery, particularly in facilitating successful implant-prosthetic rehabilitation. This systematic review explores the regenerative potential of growth factors, such as platelet-rich fibrin (PRF) and concentrated growth factors (CGFs), when combined with various types of scaffolds in bone augmentation procedures, including guided bone regeneration, split crest, sinus lift (SL), and alveolar ridge preservation. Method: A comprehensive search strategy yielded 18 relevant studies, which were analyzed for bone formation and stabilization outcomes. Results: Results indicate that PRF enhances bone regeneration and stabilization in SL and ridge augmentation procedures, while CGFs facilitate surgical techniques and augment bone. However, some studies did not report significant differences. Growth factors also demonstrate benefits in wound healing, reducing bone resorption, and enhancing socket preservation. Conclusion: Despite valuable insights, further research is needed to comprehensively understand the characteristics of growth factors in various surgical interventions, ensuring informed decision-making in bone regeneration surgery.
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Affiliation(s)
- Francesco Inchingolo
- Interdisciplinary Department of Medicine, Università degli Studi di Bari Aldo Moro, Bari 70124, Italy
| | - Angelo Michele Inchingolo
- Interdisciplinary Department of Medicine, Università degli Studi di Bari Aldo Moro, Bari 70124, Italy
| | - Giulia Latini
- Interdisciplinary Department of Medicine, Università degli Studi di Bari Aldo Moro, Bari 70124, Italy
| | - Elisabetta de Ruvo
- Interdisciplinary Department of Medicine, Università degli Studi di Bari Aldo Moro, Bari 70124, Italy
| | - Merigrazia Campanelli
- Interdisciplinary Department of Medicine, Università degli Studi di Bari Aldo Moro, Bari 70124, Italy
| | - Andrea Palermo
- College of Medicine and Dentistry, Birmingham B4 6BN, UK
| | - Massimo Del Fabbro
- Department of Biomedical Surgical and Dental Sciences, University of Milan, Milan, Italy
| | - Marco Di Blasio
- Department of Biomedical Surgical and Dental Sciences, University of Milan, Milan, Italy
| | - Alessio Danilo Inchingolo
- Interdisciplinary Department of Medicine, Università degli Studi di Bari Aldo Moro, Bari 70124, Italy
| | - Gianna Dipalma
- Interdisciplinary Department of Medicine, Università degli Studi di Bari Aldo Moro, Bari 70124, Italy
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Sreeparvathy R, Belludi SA, Prabhu A. Platelet Rich Fibrin Matrix (PRFM) and Peripheral Blood Mesenchymal Stem Cells (PBMSCs) in the management of intraosseous defects - A randomized clinical trial. J Appl Oral Sci 2024; 32:e20230442. [PMID: 39109750 PMCID: PMC11321798 DOI: 10.1590/1678-7757-2023-0442] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 12/10/2023] [Revised: 05/13/2024] [Accepted: 05/24/2024] [Indexed: 08/15/2024] Open
Abstract
OBJECTIVE A combination of peripheral blood mesenchymal stem cells (PBMSCs) and platelet rich fibrin matrix (PRFM) could be a probable periodontal regenerative material with the synergy of the added benefits of each material. This randomized controlled clinical trial aimed to evaluate the regenerative capacity of supercell (PRFM and PBMSCs) compared with that of PRFM alone in human periodontal mandibular intraosseous defects (IOD). METHODOLOGY This study included 17 patients of both sexes (12 men, 5 women) aged 30-55 years (mean age = 37.7±4.4 years) who fulfilled the inclusion criteria (radiographic and clinical evaluation for bilateral IOD with probing pocket depth (PPD ≥ 6 mm). A split-mouth design was used in each patient. A total of 34 sites in the mandibular arch randomly received PRFM alone + open flap debridement (OFD) [Control sites] or supercell (PRFM+PBMSCs) + OFD [Test sites]. The clinical parameters plaque index (PI), gingival index (GI), PPD, clinical attachment level (CAL), and in the radiographic parameters; defect depth (DD) and defect fill percentage (DFP) were recorded at baseline, 3 and 6 months postoperatively. Early wound healing index (EHI) was used at 1 week to assess wound healing ability. RESULTS At 6 months, radiographic parameters revealed significant reduction in DD (P<0.001) and significant DFP values in the test group compared with the control group. The supercell showed significant improvement in PPD and CAL at the end of 6 months (P<0.001). EHI scores at 1 week showed no statistically significant difference between the test and control groups. CONCLUSION Supercell can be considered a regenerative material in the treatment of periodontal IODs.
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Affiliation(s)
- R Sreeparvathy
- K.L.E Society's Institute of Dental Sciences, Department of Periodontics, Bengaluru, India
| | - Sphoorthi Anup Belludi
- K.L.E Society's Institute of Dental Sciences, Department of Periodontics, Bengaluru, India
| | - Ashwin Prabhu
- K.L.E Society's Institute of Dental Sciences, Department of Periodontics, Bengaluru, India
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Desai KN, Thakkar RR, Patel J, Gupta E, Trivedi N, Sampat D. Comparative Evaluation of DFDBA versus PRF with DFDBA in Treatment of Grade-II Furcation Defects - A Clinical Trial. JOURNAL OF PHARMACY AND BIOALLIED SCIENCES 2024; 16:S794-S796. [PMID: 38595476 PMCID: PMC11001046 DOI: 10.4103/jpbs.jpbs_1016_23] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 10/09/2023] [Revised: 10/18/2023] [Accepted: 10/20/2023] [Indexed: 04/11/2024] Open
Abstract
Background In order to determine whether a method is more successful for treating a grade-II furcation deficiency, this randomized trial will compare demineralized freeze-dried bone allograft (DFDBA) to platelet-rich fibrin with DFDBA. Materials and Methods Twenty systematically healthy patients between the ages of 30 and 60 with a grade-II furcation were evaluated pre and postoperatively for changes in the modified plaque index, probing depth, relative vertical and horizontal clinical attachment level, gingival marginal level, and radiographic bone defect. Results The test group significantly outperformed the control group on all clinical and radiological measures. Conclusion The experimental group improved at both clinical attachment levels and had a higher decrease in probing depth than the control group did.
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Affiliation(s)
- Kinjal N. Desai
- Department of Periodontology and Implantology, Siddhpur Dental College and Hospital, Siddhpur, Gujarat, India
| | - Rohit R. Thakkar
- Department of Periodontology and Implantology, Siddhpur Dental College and Hospital, Siddhpur, Gujarat, India
| | - Jalpa Patel
- Department of Periodontology and Implantology, Narsinhbhai Patel Dental College and Hospital, Sankalchand Patel University, Visnagar, Gujarat, India
| | - Ekta Gupta
- Department of Orthodontics and Dentofacial Orthopedics, Siddhpur Dental College and Hospital, Patan, Gujarat, India
| | - Namrata Trivedi
- Senior Lecturer, Department of Periodontology and Implantology, College of Dental Science and Hospital, Amargadh, Gujarat, India
| | - Dipali Sampat
- Clinical Practitioner, Department of Periodontology and Implantology, Bhavtarini Charitable Trust Gondal, Gujarat, India
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Wardani A, Tran B, Duterre M, Larabi I, Waskiewicz K, Louryan S, Evrard L. Healing of particulate allografts mixed with platelet concentrates in ridge preservation and sinus lift: a prospective histomorphometric study. Morphologie 2023:S1286-0115(23)00027-9. [PMID: 36997466 DOI: 10.1016/j.morpho.2023.03.001] [Citation(s) in RCA: 2] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 11/24/2022] [Revised: 03/01/2023] [Accepted: 03/14/2023] [Indexed: 03/29/2023]
Abstract
The objective of the present study was to evaluate the bone quality of sinus and alveolar grafts following filling with particulate allogenous bone (DFDBA 300-500μm) and platelet concentrate (platelet-rich fibrin, PRF). A prospective interventional clinical study was carried out. A total of 40 bone cores, 2mm in diameter, were taken from 21 patients: 22 from grafted alveoli, 7 from grafted sinus sites, and 11 from native bone used as a control. Fixed, paraffin-embedded samples were subjected to histological staining with hematoxylin-eosin and Masson's trichrome. Bone maturity of the samples was evaluated by two independent operators using histomorphometric analysis. There existed a greater proportion of lamellar neoformed bone than woven neoformed bone as the healing time increased. Moreover, there was also an increasing proportion of newly formed bone in the grafted sockets as a function of healing time (average: 41.22% ≤ 5 months, 55.89% ˃ 5 months). Resorption of DFDBA particles also appears to be correlated with healing time in the grafted socket (average: 15.43 ≤ 5 months, 13.72% ˃ 5 months). In conclusion, performing sinus lift and alveolar socket preservation techniques using DFDBA and PRF results in high quality, mature bone tissue according to histological criteria.
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Affiliation(s)
- A Wardani
- Department of dentistry, orthodontics, stomatology, oral and maxillofacial surgery, faculty of medicine, université libre de Bruxelles, hôpital Erasme, Bruxelles, Belgium.
| | - B Tran
- Department of dentistry, orthodontics, stomatology, oral and maxillofacial surgery, faculty of medicine, université libre de Bruxelles, hôpital Erasme, Bruxelles, Belgium.
| | - M Duterre
- Laboratory of anatomy, biomechanics and organogenesis, Bruxelles, Belgium.
| | - I Larabi
- Department of dentistry, orthodontics, stomatology, oral and maxillofacial surgery, faculty of medicine, université libre de Bruxelles, hôpital Erasme, Bruxelles, Belgium.
| | - K Waskiewicz
- Department of dentistry, orthodontics, stomatology, oral and maxillofacial surgery, faculty of medicine, université libre de Bruxelles, hôpital Erasme, Bruxelles, Belgium.
| | - S Louryan
- Laboratory of anatomy, biomechanics and organogenesis, Bruxelles, Belgium.
| | - L Evrard
- Department of dentistry, orthodontics, stomatology, oral and maxillofacial surgery, faculty of medicine, université libre de Bruxelles, hôpital Erasme, Bruxelles, Belgium.
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Keshavarzi F, Ahrari F, Seyedmajidi M, Ziaei M, Bijani A, Jenabian N. Clinical, histological, and histomorphometrical comparison of CenoBone ® with and without plasma rich in growth factor for edentulous ridge preservation in the dental sockets. Dent Res J (Isfahan) 2023; 20:26. [PMID: 36960023 PMCID: PMC10028575 DOI: 10.4103/1735-3327.369628] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 07/02/2021] [Revised: 07/08/2022] [Accepted: 12/20/2022] [Indexed: 03/25/2023] Open
Abstract
Background The aim of this study was to compare the clinical, histological, and histomorphometrical outcomes of CenoBone® allograft with and without plasma rich in growth factor (PRGF) for the preservation of edentulous ridge in the dental sockets. Materials and Methods This study is experimental clinical trial that 14 dental sockets were included the sockets required ridge preservation followed by implant placement in the premolar and molar of the mandible. After extraction of the teeth, the CenoBone® allograft and PRGF were used in the test group and CenoBone® allograft was used alone in the control group. During the first stage of surgery and 5 months later, in the second stage of surgery (implant placement), the vertical changes of the ridge were measured. Furthermore, using Core-Biopsy in the second stage of surgery, criteria of histologic and histomorphometric were determined. Data were analyzed with t-test, Mann-Whitney U-test, and Fisher's exact test at the level of significance of P < 0.05. Results The mean trabecular thickness in the test group (52.18 ± 5.53) was significantly higher than that in the control group (41.53 ± 10.40) (P = 0.344). However, there were no significant differences in the mean values of vertical bone absorption, bone percentage, remaining biomaterials, inflammation, and blood vessels between the two groups. There was no case of foreign body reaction and the bone was vital in all the cases and in direct contact with the biomaterial. Conclusion Although CenoBone® allograft with PRGF was effective in some histomorphometric factors such as trabecular thickness, it did not lead to significant clinical changes.
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Affiliation(s)
- Farshad Keshavarzi
- Department of Periodontology, Reaserch Committte, Babol University of Medical Sciences, Babol, Iran
| | - Fateme Ahrari
- Department of Periodontology, Reaserch Committte, Babol University of Medical Sciences, Babol, Iran
| | - Maryam Seyedmajidi
- Dental Materials Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran
| | - Maryam Ziaei
- Department of Pedodontics, Faculty of Dentistry, Babol University of Medical Sciences, Babol, Iran
| | - Ali Bijani
- Social Determinants of Health Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran
| | - Niloofar Jenabian
- Oral Health Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran
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Wang J, Sun Y, Liu Y, Yu J, Sun X, Wang L, Zhou Y. Effects of platelet-rich fibrin on osteogenic differentiation of Schneiderian membrane derived mesenchymal stem cells and bone formation in maxillary sinus. Cell Commun Signal 2022; 20:88. [PMID: 35705970 PMCID: PMC9202141 DOI: 10.1186/s12964-022-00844-0] [Citation(s) in RCA: 16] [Impact Index Per Article: 5.3] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 10/13/2021] [Accepted: 02/11/2022] [Indexed: 01/24/2023] Open
Abstract
BACKGROUND The existence of mesenchymal stem cells (MSCs) in Schneiderian membrane has not been determined. The aim of this study is to investigate whether there are MSCs in Schneiderian membrane, and the effect of platelet-rich fibrin (PRF) on osteogenic differentiation of these cells and on new bone formation in maxillary sinus after maxillary sinus floor elevation. METHODS Schneiderian membrane derived mesenchymal stem cells (SM-MSCs) were isolated from rabbit maxillary sinus. Cells were identified by flow cytometry and multipotential differentiation. Real-time cell analysis assay, fluorescence staining, transwell assay, and wound healing assay were used to determine the effects of PRF stimulation on cell proliferation and migration. The osteogenic differentiation ability of cells stimulated by PRF or osteoinductive medium was evaluated by alkaline phosphatase staining, alizarin red staining, PCR and Western blot. Equivalent volume Bio-oss and the mixture of Bio-oss and PRF were used as bone graft materials for maxillary sinus floor elevation. Micro-CT, bone double-staining, HE staining, Masson staining, and toluidine blue staining were used to evaluate the osteogenic effect in 8 and 12 weeks after surgery. RESULTS The cell surface markers were positive for expression of CD90, CD105, and negative for expression of CD34, CD45. SM-MSCs had the ability of osteogenic, adipogenic and chondrogenic differentiation. PRF could stimulate proliferation, migration and osteogenic differentiation of SM-MSCs, which was achieved by up-regulating ERK 1/2 signaling pathway. PRF could accelerate the formation of new bone in maxillary sinus and increase the amount of new bone formation. CONCLUSIONS MSCs existed in Schneiderian membrane, and PRF stimulation could promote cell proliferation, migration and osteogenic differentiation. The application of PRF in maxillary sinus floor elevation could accelerate bone healing and increase the quantity and quality of new bone. PRF, as autologous graft materials, might offer a promising strategy for the clinical bone formation during MSFE procedure. Video Abstract.
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Affiliation(s)
- Jia Wang
- Department of Oral Implantology, Hospital of Stomatology, Jilin University, Changchun, 130021 China
- Stomatology Hospital, School of Stomatology, Zhejiang University School of Medicine, Zhejiang Provincial Clinical Research Center for Oral Diseases, Key Laboratory of Oral Biomedical Research of Zhejiang Province, Cancer Center of Zhejiang University, Hangzhou, 310006 China
| | - Yue Sun
- Department of Oral Implantology, Hospital of Stomatology, Jilin University, Changchun, 130021 China
| | - Yiping Liu
- Department of Oral Implantology, Hospital of Stomatology, Jilin University, Changchun, 130021 China
| | - Jize Yu
- Department of Oral Implantology, Hospital of Stomatology, Jilin University, Changchun, 130021 China
| | - Xiaolin Sun
- Department of Oral Implantology, Hospital of Stomatology, Jilin University, Changchun, 130021 China
| | - Lin Wang
- Department of Oral Implantology, Hospital of Stomatology, Jilin University, Changchun, 130021 China
| | - Yanmin Zhou
- Department of Oral Implantology, Hospital of Stomatology, Jilin University, Changchun, 130021 China
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Shahbaz Alam M, Dhiman A, Jain V, Bhutia O, Pruthi G. Vertical Bone Implant Contact Around Anterior Immediate Implants and Their Stability After Using Either Alloplast or L-PRF or Both in Peri-Implant Gap: A Prospective Randomized Trial. J Maxillofac Oral Surg 2022; 21:533-541. [PMID: 35712396 PMCID: PMC9192842 DOI: 10.1007/s12663-020-01426-8] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/20/2020] [Accepted: 07/22/2020] [Indexed: 10/23/2022] Open
Abstract
Purpose Various graft materials have been studied for filling peri-implant gap (PIG), but there was no similar randomized clinical trial to evaluate the effect of Platelet-rich fibrin or alloplast or their combination on vertical bone implant contact (BIC) around immediate implants and their stability over a period of 1 year. Methods Immediate implants were placed in maxillary anterior region of 30 subjects (n = 10). Either alloplast (group I) or L-PRF (group II) or both (group III) were used to fill the PIG following randomization chart. Vertical BIC was measured on peri-apical radiographs which were taken immediately after placement, after 3, 6 and 12 months using Image J software. Periotest was used to measure the implant stability at the time of implant placement, at 3, 6 and 12 months after implantation. Results The comparison of distance from implant shoulder to the first visible bone-to-implant contact (IS-BIC) in each group showed statistically significant bone formation on mesial and distal sides over a 1 year period (p < 0.05). There was no significant difference in IS-BIC distance among the three groups at 1 year (p > 0.05). Periotest values showed that there was significant improvement in implant stability in all groups in 1-year period. On intergroup comparison, the mean difference of periotest values was statistically non-significant among three groups (p > 0.05). Conclusions All the graft materials were effective in promoting osseointegration when used as PIG filling materials alone or in combination around immediate implants in maxillary anterior region. CTRI No REF/2015/06/009200.
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Affiliation(s)
- Md Shahbaz Alam
- Center for Dental Education and Research, All India Institute of Medical Sciences, New Delhi, 110029 India
| | - Arjun Dhiman
- Center for Dental Education and Research, All India Institute of Medical Sciences, New Delhi, 110029 India
| | - Veena Jain
- Department of Prosthodontics, CDER, AIIMS, New Delhi, 110029 India
| | - Ongkila Bhutia
- Department of Oral and Maxillofacial Surgery, CDER, AIIMS, New Delhi, 110029 India
| | - Gunjan Pruthi
- Department of Prosthodontics, Oral Health Sciences Center, PGIMER, Chandigarh, India
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Pascawinata A, Bakar A. Combination of Nanocrystalline Hydroxyapatite and Injectable Platelet-Rich Fibrin on Bone Graft Materials for Alveolar Bone Preservation. Open Access Maced J Med Sci 2022. [DOI: 10.3889/oamjms.2022.8762] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/05/2022] Open
Abstract
Alveolar bone resorption is one of post-extraction complications with a reduction in the dimensions and quality of the alveolar bone, which will make it challenging to install dental implants in the future. The resorption can be prevented by preserving the alveolar bone using bone grafts. Nanocrystalline hydroxyapatite (HA) is a widely developed material as a bone graft. However, there are still some limitations because it only has osteoconductive properties. The addition of injectable platelet-rich fibrin to HA can increase this material’s osteoinductive, antibacterial, and anti-inflammatory properties, making it suitable for use as bone graft material for the preservation of alveolar bone.
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Walia KD, Belludi SA, Pradhan N, Jain V, Shaik S. Evaluation of Platelet-Rich Fibrin Matrix as a Regenerative Material in the Surgical Management of Human Periodontal Intraosseous Defects - A Randomized Controlled Trial. Contemp Clin Dent 2022; 13:9-17. [PMID: 35466289 PMCID: PMC9030306 DOI: 10.4103/ccd.ccd_832_20] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/27/2020] [Revised: 12/26/2020] [Accepted: 04/03/2021] [Indexed: 11/07/2022] Open
Abstract
Background Platelet-rich fibrin matrix (PRFM) has not been extensively studied as other platelet concentrates such as Choukron's platelet-rich fibrin (PRF). This randomized controlled trial aimed to evaluate PRFM regenerative ability in human periodontal intrabony defects. Materials and Methods Fifteen patients (age: 30-55 years) having probing pocket depth (PPD) ≥6 mm, and radiographic evidence of bilateral vertical intrabony defects were recruited. A split-mouth design was used in each patient; one quadrant of the arch was treated with open flap debridement (OFD) alone (control group) and the other quadrant with OFD + PRFM (test group). The PRFM was prepared by dual-spin technology using a patented thixotropic separator gel. Outcome measures comprising plaque index, gingival index (GI), PPD, clinical attachment level (CAL), depth of the defect, defect fill (DF), and percentage of DF (PDF) were recorded at baseline, after 3 months and 6 months. The parameters were applicably analyzed using the Friedman test, Fisher's exact test, t-test, paired t-test, repeated measures ANOVA, and Post Hoc-Bonferroni correction. Results The GI, a net reduction in CAL, and PPD of the test group were significantly better than the control group at 3 months and 6 months (P < 0.05), while DF and PDF showed significant results at 6 months (P < 0.05). A consistent early wound healing index of 1 at 1 week was displayed in the test group (66.7%) in comparison to the control group (33.7%). Conclusion PRFM can be a clinically significant periodontal regenerative material in the treatment of vertical intraosseous defects.
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Affiliation(s)
| | - Sphoorthi Anup Belludi
- Department of Periodontics, K.L.E Society's Institute of Dental Sciences, Bengaluru, Karnataka, India
| | - Neha Pradhan
- Department of Periodontics, K.L.E Society's Institute of Dental Sciences, Bengaluru, Karnataka, India
| | - Vipin Jain
- Department of Public Health Dentistry, K.L.E Society's Institute of Dental Sciences, Bengaluru, Karnataka, India
| | - Sharaz Shaik
- Department of Prosthetic Dentistry, College of Dentistry, King Khalid University, Abha, Saudi Arabia
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Brahma Prasad Chary NO, Raju MS, Suresh Sajjan MC, Gottumukkala SN, Manyam R. Comparison of quality of bone and insertion torque values of early implants placed at 6 and 8 weeks in sockets preserved with advanced platelet-rich fibrin: A randomized controlled trial. J Indian Prosthodont Soc 2021; 21:366-374. [PMID: 34810364 PMCID: PMC8617450 DOI: 10.4103/jips.jips_331_21] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/04/2022] Open
Abstract
Aim Successful functional and esthetic rehabilitation of edentulous jaws with implants depends on the optimal timing of placement, surgical protocol, materials used, cost-effectiveness, and satisfying patient needs. Increasing demand for shorter treatment times necessitates the immediate placement protocol. However, researchers have demonstrateda higher failure rate. A-PRF (Advanced platelet-rich fibrin) has exhibited accelerated bone regeneration potential. Early implant placement with a limited healing period, along with A-PRF, can be beneficial over conventional and immediate implant placement. Settings and Design This prospective randomized clinical trial aims to assess the outcome of early implant placement in sockets preserved using A-PRF at six weeks and eight weeks of post-extraction. Two groups of 10 participantseach were formed. All patients underwent atraumatic extraction and socket preservation using A-PRF. Materials and Methods A Partial-thickness pedicle graft was raised, and the extraction socket wasclosed. Implants wereplaced in at six and eight weeks of post-extraction in group A group B, respectively. The histomorphometric analysisassessedthe bone quality present at the timeof surgery. The insertion torque values were recorded during implant placement. Statistical Analysis Used The obtained data were statistically analyzed using parametric tests, namely independent T-test for intergroup comparison. Results T-test for torque values indicated a significantly higher torque value at eight weeks. The meanhistomorphometric value showeda significantly higher percentage of bone formation at eight weeks than at six weeks (P = 0.03). Conclusion Within the study's limitations, early implant placement in extraction sockets preserved with A-PRF had significantly higher insertion torque values and predictable bone at eight weeks compared to six weeks.
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Affiliation(s)
| | - M Satyanarayana Raju
- Department of Prosthodontics and Implantology, Vishnu Dental College, Bhimavaram, Andhra Pradesh, India
| | - M C Suresh Sajjan
- Department of Prosthodontics and Implantology, Vishnu Dental College, Bhimavaram, Andhra Pradesh, India
| | - Sruthima Nvs Gottumukkala
- Department of Periodontics and Implantology, Vishnu Dental College, Bhimavaram, Andhra Pradesh, India
| | - Ravikanth Manyam
- Department of Oral and Maxillofacial Pathology, Vishnu Dental College, Bhimavaram, Andhra Pradesh, India
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Reisie BH, Farhad SZ, Sadeh SA. Evaluation and comparison number of gingival fibroblast and osteosarcoma cell (MG-63 cell line) adhesive to mocugraft, alloderm, and collagen membrane with or without advanced platelet-rich fibrin. Dent Res J (Isfahan) 2021; 18:82. [PMID: 34760073 PMCID: PMC8554468] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 04/06/2020] [Revised: 11/25/2020] [Accepted: 04/19/2021] [Indexed: 01/26/2023] Open
Abstract
BACKGROUND The tissue engineering has recently shown a significant progress in the fields of membranes and biosynthetic materials. Advanced platelet-rich fibrin (A-PRF) contains functional molecules that have newly shown great interest in regenerative therapies. The purpose of this study was to evaluate the effect of A-PRF on the adhesion of gingival fibroblast cells and osteosarcoma cells to different membranes. MATERIALS AND METHODS In this experimental in vitro study, three collagen, alloderm, and mucograft membranes were studied, which were cut into four 5 mm × 5 mm pieces and placed in the bottom of a 24-well culture medium. One milliliter of A-PRF was added to two wells from each group and the other two wells remained without A-PRF. The gingival fibroblasts and osteosarcoma cells were individually added to each well. The cell adhesion was studied using an electron microscope after 24 h. The data were analyzed by independent t-test, one-way analysis of variance, and least significant difference test. RESULTS In the presence of A-PRF, there was a significant higher osteoblast adhesion to collagen membrane compared to alloderm and mucograft membranes (P < 0.001). In the absence of A-PRF, adhesion of osteoblasts to collagen membrane was significantly higher than alloderm and mucograft (P = 0.019). Moreover, in the presence of A-PRF, fibroblast adhesion to collagen membrane was significantly higher than alloderm and mucograft membranes (P < 0.001). Furthermore, in the absence of A-PRF, no significant difference was found among the study groups (P = 0.830). CONCLUSION A-PRF was effective on fibroblast adhesion to the collagen membrane, which is similar to its absence. A-PRF was also found to be very effective on the adhesion of fibroblast cells to the collagen membrane, and in its absence, even less adhesion was observed compared to the other membranes. The presence or absence of A-PRF showed no significant differences in both cells' adhesion for alloderm and mucograft membranes.
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Affiliation(s)
- Bentol Hoda Reisie
- Department of Periodontics, Faculty of Dentistry, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran
| | - Shirin Zahra Farhad
- Department of Periodontics, Faculty of Dentistry, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran
| | - Shirin Amini Sadeh
- Department of Periodontics, Faculty of Dentistry, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran
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Banyatworakul P, Osathanon T, Kalpravidh C, Pavasant P, Pirarat N. Evaluation of the Use of Platelet-Rich Fibrin Xenologous Membranes Derived from Bubaline Blood in Canine Periodontal Defects. Vet Sci 2021; 8:vetsci8100210. [PMID: 34679040 PMCID: PMC8540583 DOI: 10.3390/vetsci8100210] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/14/2021] [Revised: 09/19/2021] [Accepted: 09/22/2021] [Indexed: 12/27/2022] Open
Abstract
Periodontal disease is the most common oral disease in dogs. Platelet-rich fibrin (PRF) is widely utilized to facilitate soft and hard tissue healing and has been proposed in periodontal healing in small animal treatment. However, the quality and amount of autologous PRF is compromised in animals with systemic diseases. The present study aimed to evaluate the efficacy of xenologous bubaline blood-derived PRF (bPRF) on periodontal tissue healing in canine periodontal defects. Split-mouth design was employed in twenty dogs diagnosed with periodontal disease. The defects were divided randomly into two groups: the open-flap debridement (OFD)-treated group and the OFD with bPRF (OFD+bPRF) application group. Results demonstrated that gingival index and periodontal probing depth decreased significantly in the OFD+bPRF group compared with those treated with OFD alone. Application of bPRF in periodontal defects also promoted fibrous tissue formation, as confirmed by the marked increase in fibrosis score. bPRF application significantly increased COL1A1 and PDGFB mRNA levels at day 14 compared with the baseline. Taking this evidence together, bPRF provided a favorable therapeutic modality in canine periodontal defects. bPRF could be an alternative biomaterial for the treatment of periodontal defects in dogs.
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Affiliation(s)
- Poranee Banyatworakul
- Dental Stem Cell Biology Research Unit, Faculty of Dentistry, Chulalongkorn University, Bangkok 10330, Thailand;
- Department of Pathology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand
| | - Thanaphum Osathanon
- Dental Stem Cell Biology Research Unit, Faculty of Dentistry, Chulalongkorn University, Bangkok 10330, Thailand;
- Department of Anatomy, Faculty of Dentistry, Chulalongkorn University, Bangkok 10330, Thailand;
- Correspondence: (T.O.); (N.P.)
| | - Chanin Kalpravidh
- Department of Surgery, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand;
| | - Prasit Pavasant
- Department of Anatomy, Faculty of Dentistry, Chulalongkorn University, Bangkok 10330, Thailand;
| | - Nopadon Pirarat
- Department of Pathology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand
- Wildlife Exotic and Aquatic Pathology-Research Unit, Department of Pathology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand
- Correspondence: (T.O.); (N.P.)
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El-dien AMS, Fathy S, El-din YA. Potential Bone Regenerative Effects of DFDBA, Simvastatin and Platelet Rich Fibrin, Radiographically and Histologically of Intra-Bony Periodontal Defects in White New Zealand Rabbits. Open Access Maced J Med Sci 2021. [DOI: 10.3889/oamjms.2021.5848] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/05/2022] Open
Abstract
AIM: This study aimed to evaluate and to compare the regenerative power of simvastatin, Demineralized Freeze-Dried Bone Allograft (DFDBA) allograft, platelets rich Fibrin (PRF), and a combination of these materials radiographically and histologically in the intra-bony periodontal defects in white New Zealand rabbits.
MATERIALS AND METHODS: This study was conducted on 54 defects in 27 adult male rabbits (n = 27) which were divided into three groups according to the follow-up preplanned scheduled for 1, 2, and 3 weeks. The selected materials were induced as following: A=DFDBA, B=Simvastatin, C= PRF, D=A+C, E=B+C, and F=negative (control group). The intra-bony periodontal defects were induced as the form of one osseous wall defect of 10 mm height, 4 mm depth between the first and the second molars. Then, samples were prepared for histological evaluation. Radiographic assessment was done using computed tomography radiography which was carried at different time intervals as the following baseline, 1, 2, and 3 weeks later. Statistical analysis was performed using ANOVA.
RESULTS: After evaluating the results, macroanatomy, radiographically, and histologically, it is thus confirmed that DFDBA allograft combined with PRF create the best bone regenerative results, followed by DFDBA, Simvastatin, simvastatin+ PRF, control group, and finally PRF.
CONCLUSION: All of the materials examined in this study showed different percentage in terms of bone density and bone regenerative effects. However, the best results for bone density of the DFDBA + PRF group were recorded after 3 weeks. Thus, the study concludes that a combination of DFDBA + PRF reflects the best properties of both materials in terms of bone density results of the defect. Such results are particularly significant for the selection of bone regeneration materials, and generally, for periodontal regeneration.
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Spagnoli DB, Niquette CC. Reconstruction of the Extraction Socket: Methods, Manipulations, and Management. Oral Maxillofac Surg Clin North Am 2020; 32:593-609. [PMID: 33004150 DOI: 10.1016/j.coms.2020.07.010] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 10/23/2022]
Abstract
Extensive reviews have concluded that grafting of the socket reduces bone loss regardless of product or method. However, nothing has been shown to reliably and completely maintain alveolar dimensions. We advocate a biologically driven and anatomically based approach for reconstruction of the socket. There are various socket manipulations that we have found to predictably prepare a site for dental implant. The combination of graft construct design and socket management maximizes graft success for any practitioner. Each socket should be treated individually, and products or methods used that are coincident with the complexity of the defect in question.
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Affiliation(s)
- Daniel B Spagnoli
- Private Practice, Brunswick Oral and Maxillofacial Surgery, 90 Medical Center Drive Southwest, Supply, NC 28462, USA
| | - Christopher C Niquette
- Brunswick Oral and Maxillofacial Surgery; Private Practice, Third Coast Oral and Maxillofacial Surgery, Grand Rapids, MI, USA.
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Effects of Autologous Platelet-Rich Fibrin in Post-Extraction Alveolar Sockets: A Randomized, Controlled Split-Mouth Trial in Dogs with Spontaneous Periodontal Disease. Animals (Basel) 2020; 10:ani10081343. [PMID: 32759693 PMCID: PMC7459700 DOI: 10.3390/ani10081343] [Citation(s) in RCA: 9] [Impact Index Per Article: 1.8] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 07/13/2020] [Revised: 07/29/2020] [Accepted: 07/31/2020] [Indexed: 12/17/2022] Open
Abstract
Simple Summary The effects of autologous platelet-rich fibrin were evaluated in dogs with spontaneous periodontal disease after tooth extraction. Both radiographic and histological findings attributed to the platelet-rich fibrin a potential ability to stimulate the natural process of tissue healing and regeneration of bone and soft tissues. Platelet-rich fibrin could, therefore, be considered as a simple and effective therapeutic aid in the management of post-extraction socket healing in dogs. Abstract Periodontal disease (PD) is a common inflammatory condition in dogs; in severe stages, dental extraction is frequently required. Platelet-rich fibrin (PRF) has been used in human oral surgical procedures and has been experimentally tested on post-extraction sockets in healthy dogs. This is the first split-mouth, randomized, controlled trial designed to compare post-extractive alveolar socket healing with and without topical application of PRF in canine spontaneous PD. Clinical evaluation, radiographic density, and histological scores for inflammation and regeneration were assessed at recruitment (T0) and after a three-week follow up (T1) on 12 dogs, for a total of 31 pairs of sockets. No complications or clinically evident differences between the treated sites and the control sites were observed. Comparing the radiographic densities of the extraction sites measured at T0 and T1, a significant enhancement was observed within the PRF group, but not within control group. The histological score decreased significantly from T0 to T1 within group PRF, but not within the control group; at T1, the PRF group showed a significantly lower histological score than the control group. These findings suggest that PRF could be able to stimulate the natural process of tissue healing and regeneration of post-extraction sites in dogs with spontaneous periodontal disease (PD).
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16
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Nisar N, Nilesh K, Parkar MI, Punde P. Extraction socket preservation using a collagen plug combined with platelet-rich plasma (PRP): A comparative clinico-radiographic study. J Dent Res Dent Clin Dent Prospects 2020; 14:139-145. [PMID: 32908656 PMCID: PMC7464229 DOI: 10.34172/joddd.2020.028] [Citation(s) in RCA: 6] [Impact Index Per Article: 1.2] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 05/12/2019] [Accepted: 04/16/2020] [Indexed: 11/09/2022] Open
Abstract
Background. Alveolar bone remodeling after tooth loss results in reduced ridge dimensions in horizontal and vertical planes. To prevent this, various authors have proposed different ridge preservation techniques. A collagen plug is a novel material that has shown promising results in preserving the alveolar bone. PRP has also yielded favorable outcomes in wound healing and promoted osteoinduction and osteoconduction Methods. Thirty patients of both sexes with an age range of 30-18 years requiring bilateral extraction of teeth with similar tooth root anatomy in the maxilla or mandible were included in the study. The extraction of teeth was carried out atraumatically. The patients' arches were randomly divided and labeled as the test or control sides. Bone width was measured on both sides. A collagen plug, with PRP, was placed, and the extraction socket was sutured on the test side. The control side was just sutured. A baseline RVG was taken to record the apico-coronal height. The patients were recalled after 10 days for suture removal and evaluation of wound healing. Parameters were re-evaluated at three and six months postoperatively. The data were subjected to t-test and one-way ANOVA. Results. The height of the crestal bone on the grafted side was more when compared to the non-grafted side three and six months after tooth extractions, and the difference was statically significant (P<0.001). No statistically significant difference was seen in the width of the alveolar bone three and six months after tooth extraction (P>0.05). Conclusion. Collagen and PRP provided reasonable socket preservation as simple and inexpensive options as compared to other materials.
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Affiliation(s)
- Numaan Nisar
- Department of Oral and Maxillofacial Surgery, School of Dental Sciences, Krishna Institute of Medical Sciences, Karad, India
| | - Kumar Nilesh
- Department of Oral and Maxillofacial Surgery, School of Dental Sciences, Krishna Institute of Medical Sciences, Karad, India
| | - Mushtaq Ishaq Parkar
- Department of Oral and Maxillofacial Surgery, School of Dental Sciences, Krishna Institute of Medical Sciences, Karad, India
| | - Prashant Punde
- Department of Oral and Maxillofacial Surgery, School of Dental Sciences, Krishna Institute of Medical Sciences, Karad, India
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Chou TM, Chang HP, Wang JC. Autologous platelet concentrates in maxillofacial regenerative therapy. Kaohsiung J Med Sci 2020; 36:305-310. [PMID: 32052598 DOI: 10.1002/kjm2.12192] [Citation(s) in RCA: 20] [Impact Index Per Article: 4.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 10/03/2019] [Accepted: 01/19/2020] [Indexed: 01/23/2023] Open
Abstract
Platelet concentrates have emerged as innovative autologous blood products that enhance tissue healing and regeneration in regenerative therapy. A common feature of these products is their higher than baseline platelet concentration, which improves wound healing and tissue repair. Four main categories of products can be easily defined, based on their leukocyte content and fibrin architecture: pure platelet-rich plasma, such as Cell Separator PRP or Anitua' PRGF; leukocyte- and platelet-rich plasma (L-PRP), such as PCCS or Ace PRP; pure platelet-rich fibrin (P-PRF), such as Fibrinet PRFM; and leukocyte- and platelet-rich fibrin (L-PRF), such as Choukroun's PRF. Two families contain significant concentrations of leukocytes: L-PRP and L-PRF. These four families of products have different biological signatures and mechanisms and obviously different clinical applications. An L-PRF membrane releases growth factors and matrix proteins over a period longer than 7 days, whereas a PRP gel matrix releases and disperses its growth factors in a relatively quick download. In the near future, simple and inexpensive products such as L-PRF are expected to have applications in oral-maxillofacial surgery, periodontal surgery, plastic surgery, orthopedic surgery, and sports medicine. Leukocytes substantially affect the intrinsic biology and properties of platelet concentrates, not only because they enhance immune function and antibacterial potential, but also because they have essential roles in the wound healing process. Unfortunately, their impact has been almost completely neglected in the literature. Improved understanding of the effects of leukocytes in wound healing is essential for development of new clinical applications of platelet concentrates.
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Affiliation(s)
- Tsau-Mau Chou
- School of Dentistry and Graduate Program of Dental Science, College of Dental Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan.,Department of Dentistry, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan
| | - Hong-Po Chang
- School of Dentistry and Graduate Program of Dental Science, College of Dental Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan.,Department of Dentistry, Kaohsiung Municipal Hsiao-Kang Hospital, Kaohsiung, Taiwan
| | - Jen-Chyan Wang
- School of Dentistry and Graduate Program of Dental Science, College of Dental Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan.,Department of Dentistry, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan
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18
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Chang YR, Liu C, Yin LH. [Research progress on platelet-rich fibrin derivatives]. HUA XI KOU QIANG YI XUE ZA ZHI = HUAXI KOUQIANG YIXUE ZAZHI = WEST CHINA JOURNAL OF STOMATOLOGY 2019; 37:660-665. [PMID: 31875447 DOI: 10.7518/hxkq.2019.06.016] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Subscribe] [Scholar Register] [Indexed: 11/21/2022]
Abstract
Platelet-rich plasma and platelet-rich fibrin (PRF) are widely used in the field of stomatology. Advance-ments in preparation techniques and basic research have enabled the use of PRF derivatives in oral clinical applications. The evolution, preparation techniques, biological properties, and medical research progress of PRF derivatives are reviewed in this paper.
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Affiliation(s)
- Yao-Ren Chang
- Dept. of Oral Implan-tation, Hospital of Stomatology, Lanzhou University, Lanzhou 730000, China
| | - Chun Liu
- Dept. of Oral Implan-tation, Hospital of Stomatology, Lanzhou University, Lanzhou 730000, China
| | - Li-Hua Yin
- Dept. of Oral Implan-tation, Hospital of Stomatology, Lanzhou University, Lanzhou 730000, China;Dept. of Dental Implantation, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University, Shanghai 200011, China
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19
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Feigin K, Shope B. Use of Platelet-Rich Plasma and Platelet-Rich Fibrin in Dentistry and Oral Surgery: Introduction and Review of the Literature. J Vet Dent 2019; 36:109-123. [DOI: 10.1177/0898756419876057] [Citation(s) in RCA: 22] [Impact Index Per Article: 3.7] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/22/2022]
Abstract
Platelet concentrates, mostly represented by platelet-rich plasma and platelet-rich fibrin, have gained significant interest in various medical and oral disciplines because of their potential to stimulate and boost regeneration of hard and soft tissues. Prepared from the patient’s own blood, they have been tested and used in various different surgical fields including oral and maxillofacial surgery. The effects of these biomaterials are described to be a result of the large concentration of platelets which contain a wide range of growth factors. The aim of this article is to introduce the principle and function of these platelet concentrates, to review their preparation, and to provide a comprehensive examination of the published oral and maxillofacial literature on this subject.
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20
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Chatterjee A, Debnath K. Comparative evaluation of growth factors from platelet concentrates: An in vitro study. J Indian Soc Periodontol 2019; 23:322-328. [PMID: 31367128 PMCID: PMC6628779 DOI: 10.4103/jisp.jisp_678_18] [Citation(s) in RCA: 16] [Impact Index Per Article: 2.7] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/04/2022] Open
Abstract
Aim The aim of the study was to compare and evaluate the various growth factors released for a period of 23 days from platelet rich fibrin (PRF) and platelet rich fibrin matrix (PRFM). Materials and Methods A total of 15 systemically healthy controls were recruited and 10 ml of blood sample was withdrawn from the individual. Following the standard centrifugation protocol, PRF and PRFM were prepared. The platelet-derived growth factor (PDGF), vascular endothelial growth factor (VEGF), epidermal growth factor (EGF), fibroblast growth factor (FGF), transforming growth factor (TGF), and insulin growth factor (IGF) were evaluated for 23 days. Results The PDGF released from PRFM was statistically significant from PRF till the 15th day of release. The VEGF too had an increased release up till the 15th day from PRFM group as compared to PRF, but no statistically significant difference could be obtained. EGF from the 15th day to 23rd day had a greater release from PRFM group as compared to PRF group. FGF from 7th day to 23rd day had statistically significant difference in the PRFM group as compared to PRF group. TGF and IGF had statistically significant difference in PRFM group as compared to PRF group from 11th day to 23rd day and 1st to 17th day, respectively. Conclusion The initial and robust release of GFs was seen in PRFM group at earlier days, whereas a steady and constant release of six GFs could be appreciated from PRF group upto 23rd day. Therefore, for a rapid and early healing and regeneration, both the platelet concentrates can be utilized in periodontal therapy.
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Affiliation(s)
- Anirban Chatterjee
- Department of Periodontology, The Oxford Dental College, Bengaluru, Karnataka, India
| | - Koel Debnath
- Department of Periodontology, The Oxford Dental College, Bengaluru, Karnataka, India
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21
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Quoc JB, Vang A, Evrard L. Peri-Implant Bone Loss at Implants Placed in Preserved Alveolar Bone Versus Implants Placed in Native Bone: A Retrospective Radiographic Study. Open Dent J 2018; 12:529-545. [PMID: 30197692 PMCID: PMC6110069 DOI: 10.2174/1874210601812010529] [Citation(s) in RCA: 4] [Impact Index Per Article: 0.6] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 04/12/2018] [Revised: 06/20/2018] [Accepted: 07/06/2018] [Indexed: 11/22/2022] Open
Abstract
Objectives The aim of our study was to compare peri-implant bone loss at implants placed in alveolar sockets filled with a particulate allogenous bone graft (DFDBA 300-500 µm) and platelet concentrates versus at implants placed in the native bone. Materials and Methods A retrospective clinical study was performed. A total of 84 patients were included with 247 implants for the restoration of mono and pluri-radicular teeth: 169 implants in native bone and 78 in socket-grafted bone. The peri-implant bone loss was measured by 2 independent operators at 6 and 12 months. Results The overall mesial and distal peri-implant bone losses were 0.9 ± 0.7 mm and 0.9 ± 0.8 mm at 6 months, respectively, and 1 ± 0.65 mm and 1.2 ± 0.9 mm at 12 months, respectively. In the tested group, the bone loss was 0.8 ± 0.8 mm at 6 months and 1.2 ± 0.9 mm at 12 months. In the control group, the bone loss was 1.0 ± 0.7 mm at 6 months and 0.95 ± 0.6 mm at 12 months. There were no statistically significant differences in bone loss between the two groups. Taking both groups together, there were no statistically significant difference in bone loss between patients with or without histories of periodontitis, but there was a statistically significant difference in bone loss between the mandible and maxilla as well as between unitary and total edentations and between partially and total edentulous patients. Conclusion At 6 and 12 months, the peri-implant bone loss in sockets preserved with DFDBA and platelet concentrates was similar to the peri-implant bone loss in native bone.
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Affiliation(s)
- Johann Bui Quoc
- Department of Dentistry, Oral and Maxillofacial Surgery, Orthodontics, Stomatology, Erasme Hospital, Université libre de Bruxelles, Brussels, Belgium
| | - Aurélie Vang
- Department of Dentistry, Oral and Maxillofacial Surgery, Orthodontics, Stomatology, Erasme Hospital, Université libre de Bruxelles, Brussels, Belgium
| | - Laurence Evrard
- Department of Dentistry, Oral and Maxillofacial Surgery, Orthodontics, Stomatology, Erasme Hospital, Université libre de Bruxelles, Brussels, Belgium
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Debnath K, Chatterjee A. Treatment of horizontal defect with and without platelet-rich fibrin matrix: A randomized comparative clinical study. J Indian Soc Periodontol 2018; 22:406-413. [PMID: 30210189 PMCID: PMC6128136 DOI: 10.4103/jisp.jisp_129_18] [Citation(s) in RCA: 5] [Impact Index Per Article: 0.7] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 02/19/2018] [Accepted: 05/14/2018] [Indexed: 11/24/2022] Open
Abstract
AIM The aim of the present study was to assess the clinical and radiographical parameters in horizontal bone defects in patients with chronic periodontitis. MATERIALS AND METHODS In this randomized, controlled clinical trial study, nine individuals with 94 sites having moderate to deep periodontitis were selected and distributed to Group A - open flap debridement (OFD), Group B - open flap debridement and intra marrow penetration (OFD + IMP) and Group C - Open flap debridement + Intramarrow penetration + platelet rich fibrin matrix (OFD + IMP + PRFM). Plaque index (PI) and gingival index (GI) were evaluated at baseline, 6 months, and 9 months after surgery. Probing pocket depth (PPD) and clinical attachment level (CAL) were recorded at baseline and 9 months after surgery. Radiographic assessment was carried out to measure the periodontal defect depth and defect fill percentage (DF%) at baseline and 9 months after the surgery using radiovisiography. RESULTS The statistical evaluation obtained after 9 months showed no significant difference between PI and GI at 9 months interval. Intergroup comparison of PPD and CAL has shown significant difference in Group C as compared to Group A and B with P < 0.05. The defect depth was statistically significant at 9 months in all the groups, and DF percentage (DF%) has shown statistically significant results in Group C as compared to A and B with P = of 0.001. CONCLUSION The addition of PRFM to horizontal type of periodontal defects has shown promising results over a 9-month follow-up period.
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Affiliation(s)
- Koel Debnath
- Department of Periodontology, The Oxford Dental College, Bengaluru, Karnataka, India
| | - Anirban Chatterjee
- Department of Periodontology, The Oxford Dental College, Bengaluru, Karnataka, India
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Kapse S, Surana S, Satish M, Hussain SE, Vyas S, Thakur D. Autologous platelet-rich fibrin: can it secure a better healing? Oral Surg Oral Med Oral Pathol Oral Radiol 2018; 127:8-18. [PMID: 30287202 DOI: 10.1016/j.oooo.2018.08.010] [Citation(s) in RCA: 30] [Impact Index Per Article: 4.3] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 05/23/2018] [Revised: 06/22/2018] [Accepted: 08/22/2018] [Indexed: 02/02/2023]
Abstract
OBJECTIVE The aim of this study was to evaluate the efficacy of platelet-rich fibrin (PRF) in the healing of impacted mandibular third molar (M3) extraction sockets. STUDY DESIGN This study included 30 patients with bilaterally symmetric impacted M3 (N = 60) requiring transalveolar extraction. All patients were assigned numbers randomly; left-sided M3 patients with odd numbers and right-sided patients with even numbers were categorized into group A (test group), and the other side of the mouth was classified as "group B" (control group). Group A M3 extraction sockets received PRF, whereas group B sockets were closed without PRF. Patients were evaluated for pain and swelling on postoperative days 1, 3, 7, and 14. Bone healing was compared on postoperative weeks 8 and 16. Analysis of variance (ANOVA) and Tukey's multiple comparison tests were applied for statistical analysis. RESULTS A total of 30 patients, ages 18 to 40 years, participated in this study. The overall postoperative pain score (visual analogue scale [VAS]) and facial swelling percentages were lower for group A compared with group B (P < .05). Early bone healing was also evident on postoperative radiographs obtained at weeks 8 and 16 in group A (P < .001). CONCLUSIONS The use of autologous PRF aids in earlier and better wound healing in a controlled manner.
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Affiliation(s)
- Sheetal Kapse
- Fellow, Maxillofacial Trauma, Association of Oral and Maxillofacial Surgeons of India, Bangalore, India); Private practitioner, Raipur, Chhattisgarh, India.
| | - Sanidhya Surana
- Private practitioner at Swasthya Sanchay Dental Clinic, Balod, Durg, Chhattisgarh, India
| | - M Satish
- Professor and Head of Department, Department of Oral and Maxillofacial Surgery at Anil Neerukonda Institute of Dental Sciences, Visakhapatnam, Andhra Pradesh, India
| | | | - Sunil Vyas
- Private practitioner, Raipur, Chhattisgarh, India
| | - Deepak Thakur
- Professor, Rungta College of Dental Sciences and Research, Bhilai, Chhattisgarh, India
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Ikono R, Mardliyati E, Agustin IT, Ulfi MMF, Andrianto D, Hasanah U, Bachtiar BM, Mardianingsih N, Bachtiar EW, Maulana NN, Rochman NT, Xianqi L, Kagami H, Nagamura-Inoue T, Tojo A. Chitosan—PRP nanosphere as a growth factors slow releasing device with superior antibacterial capability. Biomed Phys Eng Express 2018. [DOI: 10.1088/2057-1976/aac9f8] [Citation(s) in RCA: 4] [Impact Index Per Article: 0.6] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/20/2022]
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Debnath K, Chatterjee A. Clinical and histological evaluation on application of platelet concentrates on depigmented gingival epithelium. J Indian Soc Periodontol 2018; 22:150-157. [PMID: 29769770 PMCID: PMC5939023 DOI: 10.4103/jisp.jisp_342_17] [Citation(s) in RCA: 9] [Impact Index Per Article: 1.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/04/2022] Open
Abstract
Background The platelet concentrate is a windfall in the field of regenerative therapy in periodontology. It accelerates wound healing by excellent neovascularization and promoting fast cicatricle tissue remodeling. Aim This study aims to evaluate clinically and histologically accelerated effect of platelet-rich fibrin (PRF) membrane and PRF matrix (PRFM) following depigmentation procedure. Materials and Methods Eleven individuals were divided into three groups after depigmentation procedure. PRF membrane and PRFM gel were prepared as per standard protocol. Group A and B received PRF membrane and PRFM gel followed by periodontal dressing, respectively, and the only periodontal dressing was placed in Group C. The individuals were evaluated for visual analog scale (VAS) and healing index (HI) on 3rd and 5th day. Epithelization test and histologic analysis from punch biopsy were done on the 5th day. At 3rd month, reevaluation was performed. Results The intergroup statistical analysis in respect to VAS, HI, epithelization test, and histologic analysis showed a statistically significant results with P < 0.001 in Groups A and B compared to Group C. Clinical evaluation of epithelization test and histologic analysis revealed better-wound healing and moderate to no inflammatory cell infiltrate in Groups A and B, respectively, as compared to Group C, which appeared more erythematous with dense inflammatory cells. Conclusion Thus, the application of PRF membrane and PRFM gel has shown a successful approach to protect the raw wound area of depigmented sites with better patient comfort and faster healing.
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Affiliation(s)
- Koel Debnath
- Department of Periodontology, The Oxford Dental College, Bengaluru, Karnataka, India
| | - Anirban Chatterjee
- Department of Periodontology, The Oxford Dental College, Bengaluru, Karnataka, India
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S. Medikeri R, Meharwade V, M. Wate P, V. Lele S. Effect of PRF and Allograft Use on Immediate Implants at Extraction Sockets with Periapical Infection —Clinical and Cone Beam CT Findings—. THE BULLETIN OF TOKYO DENTAL COLLEGE 2018; 59:97-109. [DOI: 10.2209/tdcpublication.2017-0021] [Citation(s) in RCA: 11] [Impact Index Per Article: 1.6] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/06/2022]
Affiliation(s)
| | - Vinayak Meharwade
- Department of Periodontology, Sinhgad Dental College and Hospital, STE’s Campus
| | - Parikshit M. Wate
- Department of Periodontology, Sinhgad Dental College and Hospital, STE’s Campus
| | - Suresh V. Lele
- Department of Periodontology, Sinhgad Dental College and Hospital
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Adjunctive Effect of Autologus Platelet-Rich Fibrin to Barrier Membrane in the Treatment of Periodontal Intrabony Defects. J Craniofac Surg 2017; 27:691-6. [PMID: 27046472 DOI: 10.1097/scs.0000000000002524] [Citation(s) in RCA: 29] [Impact Index Per Article: 3.6] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 02/01/2023] Open
Abstract
BACKGROUND AND AIM Autologous platelet-rich fibrin (PRF) and barrier membranes in the treatment of intrabony defects in chronic periodontitis patients have shown significant clinical benefits. This study evaluates the additive effect of autologous PRF in combination with a barrier membrane versus the use of barrier membrane alone for the treatment of intrabony defects in chronic periodontitis patients. METHODS A randomized split-mouth design was used. Sixteen patients with 32 paired intrabony defects were included. In each patient 1 defect was treated using a resorbable collagen membrane along with PRF (test group) and the other defect by guided tissue regeneration alone (control group). The following clinical parameters were measured at baseline and after 9 months: plaque index, modified sulcus bleeding index, probing pocket depth, clinical attachment level, and gingival marginal level. The radiographic defect depth was also assessed at baseline and after 9 months. RESULTS Test group showed a statistically significant improvement for probing depth (P = 0.002), clinical attachment level (P = 0.001), and radiographic defect depth (P < 0.001) after 9 months as compared with the control sites. Radiographic defect depth reduction was 58.19 ± 13.24% in the test group as compared with 24.86 ± 9.94% reduction in the control group. CONCLUSIONS The adjunctive use of PRF in combination with barrier membrane is more effective in the treatment of intrabony defects in chronic periodontitis as compared with barrier membrane alone.
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Baniasadi B, Evrard L. Alveolar Ridge Preservation After Tooth Extraction with DFDBA and Platelet Concentrates: A Radiographic Retrospective Study. Open Dent J 2017; 11:99-108. [PMID: 28357003 PMCID: PMC5345330 DOI: 10.2174/1874210601711010099] [Citation(s) in RCA: 10] [Impact Index Per Article: 1.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 11/12/2016] [Revised: 01/06/2017] [Accepted: 01/11/2017] [Indexed: 11/22/2022] Open
Abstract
Objectives: The purpose of this study was to evaluate vertical alveolar bone loss 3 months after tooth extraction when a technique of ridge preservation was applied using a particulate demineralized freeze-dried bone allograft 300 - 500 µm associated with platelet concentrates (platelet-rich-fibrin) in the form of gel and membranes. Material and Methods: A retrospective radiological clinical study was conducted on 56 patients for whom 95 extractions had been performed immediately followed by alveolar filling. Among the patients, 17 were smokers and 16 were provided with an immediate removable temporary prosthesis after extractions. Vertical bone loss was measured radiologically by panoramic X-ray before extractions and by a computed tomography scan 3 months after, at the level of mid-buccal bone wall, by two independent observers. For statistical analysis, Student’s t-test was performed to compare the mean bone loss between mono- and pluri-radicular teeth and to compare the mean bone loss between tobacco users versus non users and finally to compare the mean bone loss between individuals that had provisional removable prosthesis and those that had not. Results: Three months after tooth extraction, the mean of vertical loss of the mid-buccal bone wall was 0.72 (SD 0.71) mm (5.53% SD 5.19). No significant difference between bone loss at mono-radicular and pluri-radicular teeth (P = 0.982) was observed. There was no significant correlation between tobacco habits and bone loss (P = 0.2), nor between provisional removable prosthesis and bone loss (P = 0.786). Conclusion: These results indicate a good potential for the technique using Demineralized Freeze-Dried Bone Allograft 300 - 500 µm and platelet concentrates in alveolar bone preservation.
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Affiliation(s)
- Behrang Baniasadi
- Department of Maxillofacial Surgery and Dentistry, Erasme Hospital, Université libre de Bruxelles, Brussels, Belgium
| | - Laurence Evrard
- Department of Maxillofacial Surgery and Dentistry, Erasme Hospital, Université libre de Bruxelles, Brussels, Belgium
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Das S, Jhingran R, Bains VK, Madan R, Srivastava R, Rizvi I. Socket preservation by beta-tri-calcium phosphate with collagen compared to platelet-rich fibrin: A clinico-radiographic study. Eur J Dent 2016; 10:264-276. [PMID: 27095909 PMCID: PMC4813448 DOI: 10.4103/1305-7456.178298] [Citation(s) in RCA: 29] [Impact Index Per Article: 3.2] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/16/2022] Open
Abstract
Objectives: This study was primarily designed to determine the clinico-radiographic efficacy of platelet-rich fibrin (PRF) and beta-tri-calcium phosphate with collagen (β-TCP-Cl) in preserving extraction sockets. Materials and Methods: For Group I (PRF), residual sockets (n = 15) were filled with autologous PRF obtained from patients' blood; and for Group II (β-TCP-Cl), residual sockets (n = 15) were filled with β-TCP-Cl. For the sockets randomly selected for Group II (β-TCP-Cl), the reshaped Resorbable Tissue Replacement cone was inserted into the socket. Results: Clinically, there was a significantly greater decrease in relative socket depth, but apposition in midcrestal height in Group II (β-TCP-Cl) as compared to Group I (PRF), whereas more decrease in buccolingual width of Group I (PRF) than Group II (β-TCP-Cl) after 6 months. Radiographically, the mean difference in socket height, residual ridge, and width (coronal, middle, and apical third of socket) after 6 months was higher in Group I (PRF) as compared to Group II (β-TCP-Cl). The mean density (in Hounsfield Units) at coronal, middle, and apical third of socket was higher in Group I (PRF) as compared to Group II (β-TCP-Cl). There were statistically significant apposition and resorption for Group I (PRF) whereas nonsignificant resorption and significant apposition for Group II (β-TCP-Cl) in buccal and lingual/palatal cortical plate, respectively, at 6 months on computerized tomography scan. Conclusion: The use of either autologous PRF or β-TCP-Cl was effective in socket preservation. Results obtained from PRF were almost similar to β-TCP-Cl; therefore being autologous, nonimmune, cost-effective, easily procurable regenerative biomaterial, PRF proves to be an insight into the future biofuel for regeneration.
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Affiliation(s)
- Swati Das
- Department of Periodontology, Saraswati Dental College, Lucknow, Uttar Pradesh, India
| | - Rajesh Jhingran
- Department of Periodontology, Saraswati Dental College, Lucknow, Uttar Pradesh, India
| | - Vivek Kumar Bains
- Department of Periodontology, Saraswati Dental College, Lucknow, Uttar Pradesh, India
| | - Rohit Madan
- Department of Periodontology, Saraswati Dental College, Lucknow, Uttar Pradesh, India
| | - Ruchi Srivastava
- Department of Periodontology, Saraswati Dental College, Lucknow, Uttar Pradesh, India
| | - Iram Rizvi
- Department of Periodontology, Saraswati Dental College, Lucknow, Uttar Pradesh, India
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Del Torto M, Enea D, Panfoli N, Filardo G, Pace N, Chiusaroli M. Hamstrings anterior cruciate ligament reconstruction with and without platelet rich fibrin matrix. Knee Surg Sports Traumatol Arthrosc 2015; 23:3614-22. [PMID: 25173508 DOI: 10.1007/s00167-014-3260-6] [Citation(s) in RCA: 23] [Impact Index Per Article: 2.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 08/29/2013] [Accepted: 08/20/2014] [Indexed: 12/14/2022]
Abstract
PURPOSE Anterior cruciate ligament (ACL) rupture is the most common complete ligamentous injury in the knee. Many studies explored ACL graft integration and maturation, but only a few assessed the application of platelet rich fibrin matrix (PRFM) as augmentation for ACL reconstruction. The main aim of this study was to test the PRFM augmentation in terms of graft-bone integration and knee stability. The secondary aim was to investigate patient-reported functional status. METHODS Prospective evaluation has been done in two consecutive series of patients who underwent ACL reconstruction with semitendinosus and gracilis (STG) grafts: 14 patients were operated with PRFM augmentation and 14 patients without PRFM augmentation. Objective clinical evaluation (Rolimeter) and MRI evaluation were performed at 1 year from surgery. Subjective evaluation (IKDC) was performed pre-operatively and at 6 months, 1 and 2 years from surgery. RESULTS A statistically significant difference was not detected between the two groups in terms of MRI and objective clinical evaluation, although PRFM-augmented patients showed a statistically significant higher clinical improvement. CONCLUSIONS The procedure described for PRFM augmentation in ACL STG reconstruction does not improve radiologic graft integration and knee stability after 1 year and should not be used by clinicians to this purpose. However, it may result in a short-term improvement of patient-reported knee function, and future research should focus on further developing PRP treatment to optimize ACL clinical outcome. LEVEL OF EVIDENCE III.
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Affiliation(s)
- M Del Torto
- Department of Orthopaedics and Traumatology, Jesi Civil Hospital, Viale Della Vittoria, 76, Jesi, AN, Italy.
| | - D Enea
- Department of Orthopaedics, Polytechnic University of Marche, Ancona, Italy
| | - N Panfoli
- Department of Orthopaedics and Traumatology, Jesi Civil Hospital, Viale Della Vittoria, 76, Jesi, AN, Italy
| | - G Filardo
- II Clinic - Biomechanics Laboratory, Rizzoli Orthopaedic Institute, Bologna, Italy
| | - N Pace
- Department of Orthopaedics and Traumatology, Jesi Civil Hospital, Viale Della Vittoria, 76, Jesi, AN, Italy
| | - M Chiusaroli
- Department of Orthopaedics and Traumatology, Jesi Civil Hospital, Viale Della Vittoria, 76, Jesi, AN, Italy
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Reddy PK, Bolla V, Koppolu P, Srujan P. Long palatal connective tissue rolled pedicle graft with demineralized freeze-dried bone allograft plus platelet-rich fibrin combination: A novel technique for ridge augmentation - Three case reports. J Indian Soc Periodontol 2015; 19:227-31. [PMID: 26015679 PMCID: PMC4439638 DOI: 10.4103/0972-124x.149932] [Citation(s) in RCA: 3] [Impact Index Per Article: 0.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 11/27/2013] [Accepted: 10/08/2014] [Indexed: 11/30/2022] Open
Abstract
Replacement of missing maxillary anterior tooth with localized residual alveolar ridge defect is challenging, considering the high esthetic demand. Various soft and hard tissue procedures were proposed to correct alveolar ridge deformities. Novel techniques have evolved in treating these ridge defects to improve function and esthetics. In the present case reports, a novel technique using long palatal connective tissue rolled pedicle graft with demineralized freeze-dried bone allografts (DFDBAs) plus Platelet-rich fibrin (PRF) combination was proposed to correct the Class III localized anterior maxillary anterior alveolar ridge defect. The present technique resulted in predictable ridge augmentation, which can be attributed to the soft and hard tissue augmentation with a connective tissue pedicle and DFDBA plus PRF combination. This technique suggests a variation in roll technique with DFDBA plus PRF and appears to promise in gaining predictable volume in the residual ridge defect and can be considered for the treatment of moderate to severe maxillary anterior ridge defects.
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Affiliation(s)
| | - Vijayalakshmi Bolla
- Department of Periodontics, Sri Sai College of Dental Surgery, Vikarabad, Telangana, India
| | - Pradeep Koppolu
- Department of Periodontics, Sri Sai College of Dental Surgery, Vikarabad, Telangana, India
| | - Peruka Srujan
- Department of Periodontics, Sri Sai College of Dental Surgery, Vikarabad, Telangana, India
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Rodella LF, Bonazza V. Platelet preparations in dentistry: How? Why? Where? When? World J Stomatol 2015; 4:39-55. [DOI: 10.5321/wjs.v4.i2.39] [Citation(s) in RCA: 2] [Impact Index Per Article: 0.2] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 02/13/2015] [Revised: 04/08/2015] [Accepted: 04/30/2015] [Indexed: 02/06/2023] Open
Abstract
The aim of this article is to review the outcomes of platelet preparations in dentistry. A structured electronic search discovered 348 articles, which described the use of autologous platelet concentrates with a relevance to clinical dentistry. Among these articles, 220 articles investigated platelet rich plasma, 99 investigated platelet rich fibrin, 22 investigated plasma rich in growth factors and 7 investigated the use of concentrated growth factors. Several studies reported beneficial treament outcomes in terms of enhanced bone and soft tissue regeneration.
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Agarwal A, Gupta ND, Jain A. Platelet rich fibrin combined with decalcified freeze-dried bone allograft for the treatment of human intrabony periodontal defects: a randomized split mouth clinical trail. Acta Odontol Scand 2015; 74:36-43. [PMID: 25972081 DOI: 10.3109/00016357.2015.1035672] [Citation(s) in RCA: 69] [Impact Index Per Article: 6.9] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/13/2022]
Abstract
OBJECTIVE Polypeptide growth factors of platelet rich fibrin (PRF) have the potential to regenerate periodontal tissues. Osteoinductive property of demineralized freeze-dried bone allograft (DFDBA) has been successfully utilized in periodontal regeneration. The aim of the present randomized, split mouth, clinical trial was to determine the additive effects of PRF with a DFDBA in the treatment of human intrabony periodontal defects. MATERIALS AND METHODS Sixty interproximal infrabony defects in 30 healthy, non-smoker patients diagnosed with chronic periodontitis were randomly assigned to PRF/DFDBA group or the DFDBA/saline. Clinical [pocket depth (PD), clinical attachment level (CAL) and gingival recession (REC)] and radiographic (bone fill, defect resolution and alveolar crest resorption) measurements were made at baseline and at a 12-month evaluation. RESULTS Compared with baseline, 12-month results indicated that both treatment modalities resulted in significant changes in all clinical and radiographic parameters. However, the PRP/DFDBA group exhibited statistically significantly greater changes compared with the DFDBA/saline group in PD (4.15 ± 0.84 vs 3.60 ± 0.51 mm), CAL (3.73 ± 0.74 vs 2.61 ± 0.68 mm), REC (0.47 ± 0.56 vs 1.00 ± 0.61 mm), bone fill (3.50 ± 0.67 vs 2.49 ± 0.64 mm) and defect resolution (3.73 ± 0.63 vs 2.75 ± 0.57 mm). CONCLUSION Observations indicate that a combination of PRF and DFDBA is more effective than DFDBA with saline for the treatment of infrabony periodontal defects.
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Affiliation(s)
- Ashish Agarwal
- a 1 Deparment of Periodontics, Institute of Dental Sciences , Bareilly, India
| | - Narinder Dev Gupta
- b 2 Department of Periodontics, Dr. Z. A. Dental College , Aligarh, India
| | - Avikal Jain
- a 1 Deparment of Periodontics, Institute of Dental Sciences , Bareilly, India
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Uraz A, Sezgin Y, Yalim M, Taner IL, Cetiner D. Comparative evaluation of platelet-rich fibrin membrane and connective tissue graft in the treatment of multiple adjacent recession defects: A clinical study. J Dent Sci 2015. [DOI: 10.1016/j.jds.2012.10.010] [Citation(s) in RCA: 15] [Impact Index Per Article: 1.5] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/29/2022] Open
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Davis VL, Abukabda AB, Radio NM, Witt-Enderby PA, Clafshenkel WP, Cairone JV, Rutkowski JL. Platelet-Rich Preparations to Improve Healing. Part I: Workable Options for Every Size Practice. J ORAL IMPLANTOL 2014; 40:500-10. [DOI: 10.1563/aaid-joi-d-12-00104] [Citation(s) in RCA: 51] [Impact Index Per Article: 4.6] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/22/2022]
Abstract
Numerous studies have demonstrated that platelet-rich preparations applied to surgical sites, injuries, or wounds are a safe and effective way to promote soft tissue healing and bone growth. Various protocols have been developed for preparing platelet-rich preparations, with subtle but important differences between them. Unfortunately, only a minority of clinicians use platelet-rich preparations, such as platelet-rich plasma and platelet-rich fibrin, in their practice, possibly due to confusion about the different methods and their advantages and disadvantages. Therefore, the different types of preparations are described to help guide the selection of the best method for any size practice. Classic methods generally require large volumes of blood and can be expensive, complicated, and time-intensive. Simpler protocols have been developed recently, which require relatively inexpensive equipment and small blood volumes and, thus, may be more applicable for small clinical practices. Platelet-rich preparations accelerate healing at earlier time points to reduce discomfort and the potential for adverse outcomes, including infection, poor wound closure, and delays in forming strong bone for subsequent procedures (such as implants). However, platelet-rich preparations may also improve long-term outcomes in patients expected to have impaired healing, such as with lifestyle choices (eg, smoking), medications (eg, steroids), diseases (eg, diabetes, osteoporosis, atherosclerosis), and aging, by supplementing the deficient wound environment to restore proper healing. Therefore, both large and small clinical practices would benefit from utilizing platelet-rich preparations to enhance healing in their patients.
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Affiliation(s)
- Vicki L Davis
- Center for Applied Research & Intellectual Property Development, Clarion University, Clarion, Pa
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Ranganathan AT, Chandran CR. Platelet-rich fibrin in the treatment of periodontal bone defects. J Contemp Dent Pract 2014; 15:372-375. [PMID: 25307824 DOI: 10.5005/jp-journals-10024-1546] [Citation(s) in RCA: 5] [Impact Index Per Article: 0.5] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 06/04/2023]
Abstract
BACKGROUND Periodontitis is characterized by the formation of true pockets, bone loss and attachment loss. Various techniques have been attempted in the past to truly regenerate the lost periodontal structures, albeit with variable outcome. In this evolution, the technique being tried out widely is the use of platelet rich concentrates, namely platelet-rich fibrin (PRF). CASE DESCRIPTION In this report, we present a case of surgical treatment of osseous bone defects namely two walled crater and dehiscence treated in posterior teeth with autologously prepared platelet rich fibrin mixed with hydroxy apatite bone graft and PRF in the form of a membrane. CONCLUSION Our results showed clinical improvements in all the clinical parameters postoperatively namely the pocket depth reduction and gain in attachment level and hence, PRF can be used alone or in combination with the bone graft to yield successful clinical results in treating periodontal osseous defects. CLINICAL SIGNIFICANCE Platelet-rich fibrin is an effective alternative to platelet-rich plasma (PRP) in reconstructing bone defects.
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Affiliation(s)
| | - Chitraa R Chandran
- Professor and Head, Department of Periodontics, Tagore Dental College, Chennai Tamil Nadu, India
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Gawai KT, Sobhana CR. Clinical evaluation of use of platelet rich plasma in bone healing. J Maxillofac Oral Surg 2014; 14:67-80. [PMID: 25729230 DOI: 10.1007/s12663-013-0605-5] [Citation(s) in RCA: 12] [Impact Index Per Article: 1.1] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/20/2013] [Accepted: 11/27/2013] [Indexed: 11/25/2022] Open
Abstract
BACKGROUND Tooth extraction is performed for a wide variety of reasons as we know. Several techniques aiming at enhancing the regeneration process in the extraction socket have been adopted such as filling the socket with autogenous bone grafts or bone substitutes. We know platelets play a central role in hemostasis and healing processes but relative contradictory effect of platelet in bone regenerating capacity have been published in different in vitro and in vivo studies. METHOD To explore this we used platelet-rich plasma (PRP) (autogenous) alone in empty extraction socket of bilateral impacted mandibular third molars. For that we selected five patients having bilateral impacted teeth. Out of two sockets one was used as intervention by filling with PRP and the other was allowed to heal without PRP. All patients were followed for clinical and radiological evaluation by using digital OPG view after 1 week, 1, 2 and 4 months period. RESULT AND CONCLUSION PRP enhanced the osteogenic response in initial bone healing at 1 month duration but there was no added benefit in late bone healing at 4 months period compared in both intervention and control groups. However PRP significantly improved the soft tissue healing in PRP treated sites compared to control group.
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Affiliation(s)
- Kailas T Gawai
- Department of Oral and Maxillofacial Surgery, Medical College Hospital, Thrissur, Kerala India
| | - C R Sobhana
- Department of Oral and Maxillofacial Surgery, Government Dental College, Thiruvananthapuram, Kerala India
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Geeta IB, Galagali G, Kulkarni S, Suran P, Noushin F. A natural meliorate: revolutionary tissue engineering in endodontics. J Clin Diagn Res 2014; 7:2644-6. [PMID: 24392430 DOI: 10.7860/jcdr/2013/6915.3638] [Citation(s) in RCA: 4] [Impact Index Per Article: 0.4] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 07/10/2013] [Accepted: 10/12/2013] [Indexed: 11/24/2022]
Abstract
Platelet-Rich Fibrin (PRF) was first described by Choukroun et al., (2001). It has been referred to as a second-generation platelet concentrate, which has been shown to have several advantages over traditionally prepared platelet-rich plasma. PRF has a physiologic architecture that is very favourable to the healing process, obtained due to the slow polymerization process. The development of platelet concentrate as bioactive surgical additives that are applied locally to promote wound healing stems from the use of fibrin adhesive. Developments in the field of tissue engineering have made the generation of artificial substitutes in several areas of medicine. Various clinical applications in endodontics include Periapical surgeries, Revascularisationprocedures, Regenerative pulpotomy, Perforation repair. This article aims to discuss the various applications of PRF in the field of Endodontics with few case reports.
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Affiliation(s)
- I B Geeta
- Professor and HOD, Department of Conservative Dentistry and Endodontics, Rajarajeswari Dental College , Bangalore, Karnataka, India
| | - Girish Galagali
- Professor and HOD, Department of Prosthodonics Including Crown and Bridge, Navodaya Dental College , Raichur, Karnataka, India
| | - Sangeeta Kulkarni
- Senior Lecturer, Department of Conservative Dentistry and Endodontics, Al Badad Dental College , Gulbarga, Karnataka, India
| | - Pushpa Suran
- Reader, Department of Conservative Dentistry and Endodontics, Government Dental College , Bellary, Karnataka, India
| | - Faizal Noushin
- Post Graduate, Department of Conservative Dentistry And Endodontics, Rajarajeswari Dental College , Bangalore, Karnataka, India
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Bolukbasi N, Ersanlı S, Keklikoglu N, Basegmez C, Ozdemir T. Sinus Augmentation With Platelet-Rich Fibrin in Combination With Bovine Bone Graft Versus Bovine Bone Graft in Combination With Collagen Membrane. J ORAL IMPLANTOL 2013; 41:586-95. [PMID: 24041418 DOI: 10.1563/aaid-joi-d-13-00129] [Citation(s) in RCA: 35] [Impact Index Per Article: 2.9] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/22/2022]
Abstract
The purpose of this study was to compare the efficacy between the use of bovine bone graft material and platelet-rich fibrin (PRF) mixture (test group) and bovine bone graft material and collagen membrane combination (control group) in 2-stage maxillary sinus augmentation. According to specific inclusion/exclusion criteria, patients treated between 2008 and 2012 were selected. Panoramic radiographs were used for radiologic assessments. To evaluate the relationship between sinus-graft height and each implant, the bone level (BL) was divided by implant length (IL). To evaluate the change in the height of grafted sinus, the grafted sinus floor above the lowest part of the original sinus height (GSH) was divided by the original sinus height (OSH). Samples taken during implant surgery were used for histologic and histomorphometric analyses. Twenty-five patients, 32 augmentation surgeries, and 66 one-stage implants were included in the study. No implant loss or complication was observed in either group. There were no statistical differences according to new bone formation (P = .61) and biomaterial remnant (P = .87). During the evaluation period, the test group showed statistically less change in the BL/IL ratio (P = .022). The difference of GSH/OSH ratio was found to be insignificant between groups (P = .093). It was observed that the grafted sinus covering the implant apex and sinus floor was above the original sinus height in both groups. It may be concluded from this study that both combinations can be successfully used for sinus augmentation. Further studies evaluating different graft materials and PRF combinations in the early phases of healing would be beneficial.
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Affiliation(s)
- Nilufer Bolukbasi
- 1 Department of Oral Implantology, Faculty of Dentistry, Istanbul University, Istanbul, Turkey
| | - Selim Ersanlı
- 1 Department of Oral Implantology, Faculty of Dentistry, Istanbul University, Istanbul, Turkey
| | - Nurullah Keklikoglu
- 2 Department of Histology and Embryology, Istanbul University, Istanbul, Turkey
| | - Cansu Basegmez
- 1 Department of Oral Implantology, Faculty of Dentistry, Istanbul University, Istanbul, Turkey
| | - Tayfun Ozdemir
- 1 Department of Oral Implantology, Faculty of Dentistry, Istanbul University, Istanbul, Turkey
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Naik B, Karunakar P, Jayadev M, Marshal VR. Role of Platelet rich fibrin in wound healing: A critical review. J Conserv Dent 2013; 16:284-93. [PMID: 23956527 PMCID: PMC3740636 DOI: 10.4103/0972-0707.114344] [Citation(s) in RCA: 140] [Impact Index Per Article: 11.7] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 12/03/2012] [Revised: 03/21/2013] [Accepted: 04/22/2013] [Indexed: 02/02/2023] Open
Abstract
AIM The aim is to review and discuss the strategies available for use of platelet rich fibrin as healing aid in dentistry. BACKGROUND Platelet rich fibrin (PRF) is a fibrin matrix in which platelet cytokines, growth factors, and cells are trapped and may be released after a certain time and that can serve as a resorbable membrane. Choukroun and his associates were amongst the pioneers for using PRF protocol in oral and maxillofacial surgery to improve bone healing in implant dentistry. Autologous PRF is considered to be a healing biomaterial, and presently, studies have shown its application in various disciplines of dentistry. MATERIALS AND METHODS By using specific keywords, electronic search of scientific papers was carried out on the entire PubMed database with custom range of 5 years. The electronic search yielded 302 papers; based on inclusion and exclusion criteria which were specifically predetermined, 72 papers were identified as suitable to the inclusion criteria and the remaining 230 papers were excluded. After adding three more selected papers through hand search, full text of all the articles retrieved and review was done. By pooling the extracted data from selected papers, the reviewed data was synthesized. CONCLUSION Recently by showing good promising results with use of the PRF, it has proved to have a good prospect for its use as healing aid in various aspects of the dentistry.
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Affiliation(s)
- Balaram Naik
- Department of Conservative Dentistry and Endodontics, Sri Dharmasthala Manjunatheshwara College of Dental Sciences, Dharwad, Karnataka, India
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Krasny M, Krasny K, Kamiński A, Zadurska M, Piekarczyk P, Fiedor P. Evaluation of safety and efficacy of radiation-sterilized bone allografts in reconstructive oral surgery. Cell Tissue Bank 2012; 14:367-74. [PMID: 23239267 PMCID: PMC3732757 DOI: 10.1007/s10561-012-9348-7] [Citation(s) in RCA: 11] [Impact Index Per Article: 0.8] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 06/15/2012] [Accepted: 11/03/2012] [Indexed: 02/07/2023]
Abstract
Bone grafting allows reconstruction of the atrophied or destroyed alveolar process. In orthopaedics and traumatology allogeneic grafting has been used to restore defects of osseous tissue for over 60 years. In order to improve safety of the graft recipient, sterilized allogeneic grafts have been use. The aim of the study was to assess the direct and long-term outcomes following augmentation of atrophied alveolar processes with the use of radiation-sterilized allogeneic bone grafts. Sixty-eight patients were surgically treated between 2004 and 2011: 29 underwent open sinus floor elevation, post-extraction alveoli augmentation was performed in 16 subjects and 23 underwent reconstruction of the atrophied alveolar process. Augmentation of bone defects used bone granulate in 63 patients and bone blocks stabilized with titanium screws in 5 patients. PRF membranes collected from the patient’s blood were also used in all the procedures. In each of the cases optimal dimensions of the alveolar process were obtained allowing embedment of BIOMET 3I dental implant/-s. In all the patients the defects were successfully restored with implant-supported prostheses. Radiation-sterilized allogeneic bone grafts proved to be safe and effective for the patients and manageable for the surgeon constituting a good alternative to autogeneic material.
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Affiliation(s)
- Marta Krasny
- Department of Orthodontics, Warsaw Medical University, ul. Nowogrodzka 59, 02-006, Warsaw, Poland.
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Abstract
PURPOSE The aim of this study is to demonstrate the clinical applicability and efficacy of an allograft for maxillary sinus augmentations in patients requiring placement of dental implants. PATIENTS AND METHODS Sixty consecutive patients underwent a total of 90 sinus augmentations. Twenty-nine were women and 31 men, with a mean age of 54 years. Twenty-six patients received a bilateral procedure and 34 unilateral. All cases were treated with the lateral wall technique. Allograft consisted of demineralized freeze-dried blocks in 6 cases, particulate in 82 cases, and a combination of both in 2 cases. In 30 patients, it was combined with platelet-rich plasma. A total of 84 implants were inserted. Bone samples of grafted areas were obtained in two patients for histological examination. RESULTS Seventy-three implants were clinically successful at the reentry time. Eleven implants in seven patients were removed between 15 days and 6 months after their placement. Seven of these implants were replaced and received prostheses as well, for an overall postloading success rate of 95.2%. Follow-up for all patients after final restoration was between 12 and 96 months. Specimen's histological evaluation revealed bone formation and evidence of inflammatory infiltrate. CONCLUSIONS Based on the findings of this study, it can be suggested that the use of the demineralized freeze-dried bone allograft from the Banco de Huesos y Tejidos Fundación Cosme y Damian for sinus augmentation is effective and constitutes a feasible therapeutic alternative for implant placement.
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Bettega G, Schir E. [Contribution of platelet concentrates to oral and maxillo-facial surgery]. ACTA ACUST UNITED AC 2012; 113:205-11. [PMID: 22818655 DOI: 10.1016/j.stomax.2012.05.007] [Citation(s) in RCA: 5] [Impact Index Per Article: 0.4] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 05/02/2012] [Accepted: 05/25/2012] [Indexed: 01/30/2023]
Abstract
INTRODUCTION We evaluated the clinical contribution of platelet concentrates to oral and maxillo-facial surgery. MATERIAL AND METHOD This bibliographic research was made using the PubMed MeSH database with the following keywords: "platelet rich fibrin" (PRF), "platelet rich plasma" (PRP), "bone", "facial bone", "dental implant", and "blood platelet". The research was made without any date or language limitation since English summaries were available. All summaries were read to evaluate the relevance of the article. Only original articles and case reports were considered. The articles were classified as "in vitro studies", "animal experiments", or "clinical studies". The research was stopped on March 22, 2012. RESULTS One hundred and sixty-nine articles were validated after excluding irrelevant articles, reviews, technical notes, and articles without English or French summaries. Seventeen were in vitro studies, 61 animal experiments, and 91 clinical studies. One hundred and ten complete articles were read to complete summary data. The data of in vitro studies univocally supports of using platelet concentrates. The data from animal experiment studies was less consensual and the validity of animal models was contested. The disparity of clinical study designs and the lack of rigorous methodology did not allow clearly determining platelet concentrate benefits for oral and maxillo-facial surgery. DISCUSSION PRF or PRP clinical benefit has not been clearly demonstrated yet. French regulations relative to their use should be clarified.
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Affiliation(s)
- G Bettega
- Service de chirurgie plastique et maxillo-faciale, hôpital A.-Michallon, BP 217, 38043 Grenoble cedex, France.
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Dozza B, Gobbi G, Lucarelli E, Pierini M, Di Bella C, Frisoni T, Tazzari PL, Ricci F, Mirandola P, Carubbi C, Giannini S, Donati D, Vitale M. A rapid method for obtaining mesenchymal stem cells and platelets from bone marrow aspirate. J Tissue Eng Regen Med 2012; 8:483-92. [PMID: 22715158 DOI: 10.1002/term.1551] [Citation(s) in RCA: 5] [Impact Index Per Article: 0.4] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 07/08/2011] [Revised: 02/13/2012] [Accepted: 05/16/2012] [Indexed: 01/06/2023]
Abstract
Mesenchymal stem cells (MSCs) and platelet-rich plasma (PRP) are currently used alone or in combination for therapeutic applications especially for bone repair. We tested whether MSCs can be isolated from bone marrow (BM) aspirate using a commercially available kit commonly used to obtain PRP from peripheral blood (PB). Results revealed that mononuclear cells and platelets from both PB and BM could be efficiently isolated by obtaining a mononuclear and platelet rich fraction (PB-MPRF and BM-MPRF, respectively). Starting with comparable volumes, the number of platelets increased 1.5-fold in BM-MPRF compared to PB-MPRF. The number of clonogenic cells in BM-MPRF samples was significantly higher than whole BM samples as revealed by CFU-F assay (54.92 ± 8.55 CFU-F/1.5 x 10(5) nucleated cells and 32.50 ± 12.43 CFU-F/1.5 x 10(5) nucleated cells, respectively). Cells isolated from BM-MPRF after in vitro expansion fulfilled the definition of MSCs by phenotypic criteria, and differentiated along osteogenic, adipogenic and chondrogenic lineages following induction. Results showed that the kit isolated MSCs and platelets from BM aspirate. Isolated MSCs were further expanded in a laboratory and BM-MPRF was used clinically following BM withdrawal for rapid intra-operative cell therapy for the treatment of bone defects.
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Affiliation(s)
- Barbara Dozza
- Bone Regeneration Laboratory, 2nd Clinic of Orthopaedics and Traumatology, Rizzoli Orthopaedic Institute, Bologna, Italy
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Girish Rao S, Bhat P, Nagesh KS, Rao GHR, Mirle B, Kharbhari L, Gangaprasad B. Bone regeneration in extraction sockets with autologous platelet rich fibrin gel. J Maxillofac Oral Surg 2012; 12:11-6. [PMID: 24431807 DOI: 10.1007/s12663-012-0370-x] [Citation(s) in RCA: 33] [Impact Index Per Article: 2.5] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 01/13/2012] [Accepted: 03/05/2012] [Indexed: 11/29/2022] Open
Abstract
AIM To evaluate the effects of autologous platelet rich fibrin gel (PRF gel) on bone regeneration following extraction. MATERIALS AND METHODS The study design was approved by the Institutional Ethical Committee. Study sample consisting of a total of 22 patients requiring bilateral transalveolar third molar extractions were included after written informed consent. One side was randomly chosen as case and the other side was the control. Autologous PRF gel was prepared from Fresh blood obtained from the patient. The PRF gel was placed in the extraction site and primary closure was obtained. The patient was called for a follow up on the first post op day, 1st week, one month, three month and six months post op. Regeneration of bone was measured using serial radiographs (RVG) at immediate post op, one, three and six months. This was then compared with the bone regeneration seen in the control group, with the radiographs taken at same intervals, to estimate the difference in bone regeneration if any. RVGs were assessed for amount of radiologic bone filling by the method described by Matteo Chiapasco et al. RESULTS AND CONCLUSION Higher mean pixels was recorded in cases compared to controls at all the time intervals viz., immediate post op, 1 month post op, 3 months post op and 6 months post op. However, the difference in the mean pixels recorded between the two groups was not statistically significant (P > 0.05). For complete analysis, further follow up of the present patients and a larger sample size is required to obtain a conclusive result of the Bone Regeneration in extraction sockets with PRF gel.
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Affiliation(s)
- S Girish Rao
- Department of Oral and Maxillofacial Surgery, DAPMRV Dental College & Hospital, Bangalore, 560078 India
| | - Preethi Bhat
- Department of Oral and Maxillofacial Surgery, DAPMRV Dental College & Hospital, Bangalore, 560078 India
| | - K S Nagesh
- Department of Oral Medicine and Radiology, DAPMRV Dental College & Hospital, Bangalore, 560078 India
| | - Gundu H R Rao
- Department of Biomedical Engineering Institute, University of Minnesota, Minneapolis, MN USA
| | - Bharthi Mirle
- Department of Pathology, DAPMRV Dental College & Hospital, Bangalore, 560078 India
| | - Lubna Kharbhari
- Department of Oral and Maxillofacial Surgery, DAPMRV Dental College & Hospital, Bangalore, 560078 India
| | - B Gangaprasad
- Department of Oral and Maxillofacial Surgery, DAPMRV Dental College & Hospital, Bangalore, 560078 India
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Postextraction osteotome sinus floor elevation technique using plasma-rich growth factors. IMPLANT DENT 2012; 20:418-24. [PMID: 21986454 DOI: 10.1097/id.0b013e3182354063] [Citation(s) in RCA: 13] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/26/2022]
Abstract
PURPOSE To evaluate a modified osteotome sinus floor elevation technique with immediate postextraction implant placement and the adjunct of a platelet-derived fibrin plug for the rehabilitation of maxillary premolar sites. MATERIAL AND METHODS Fifteen patients with nonrestorable fractured endodontically treated maxillary premolars were consecutively enrolled in the study. After extraction, the membrane was gradually elevated using osteotomes, with the interposition of a fibrin clot. Subsequently, an implant embedded with plasma very rich in growth factors was inserted. RESULTS No implant failed after a mean follow-up of 35 months. The average membrane lift was 2.9 ± 0.8 mm. After 1 year of loading, marginal bone loss averaged 0.36 ± 0.19 mm. No postoperative symptoms were reported. All patients reported full satisfaction for mastication function, phonetics, and aesthetics. CONCLUSIONS The present technique represents a viable option for the rehabilitation of fresh postextraction maxillary premolar sockets.
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Roy S, Driggs J, Elgharably H, Biswas S, Findley M, Khanna S, Gnyawali U, Bergdall VK, Sen CK. Platelet-rich fibrin matrix improves wound angiogenesis via inducing endothelial cell proliferation. Wound Repair Regen 2012; 19:753-66. [PMID: 22092846 DOI: 10.1111/j.1524-475x.2011.00740.x] [Citation(s) in RCA: 112] [Impact Index Per Article: 8.6] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/18/2022]
Abstract
The economic, social, and public health burden of chronic ulcers and other compromised wounds is enormous and rapidly increasing with the aging population. The growth factors derived from platelets play an important role in tissue remodeling including neovascularization. Platelet-rich plasma (PRP) has been utilized and studied for the last four decades. Platelet gel and fibrin sealant, derived from PRP mixed with thrombin and calcium chloride, have been exogenously applied to tissues to promote wound healing, bone growth, hemostasis, and tissue sealing. In this study, we first characterized recovery and viability of as well as growth factor release from platelets in a novel preparation of platelet gel and fibrin matrix, namely platelet-rich fibrin matrix (PRFM). Next, the effect of PRFM application in a delayed model of ischemic wound angiogenesis was investigated. The study, for the first time, shows the kinetics of the viability of platelet-embedded fibrin matrix. A slow and steady release of growth factors from PRFM was observed. The vascular endothelial growth factor released from PRFM was primarily responsible for endothelial mitogenic response via extracellular signal-regulated protein kinase activation pathway. Finally, this preparation of PRFM effectively induced endothelial cell proliferation and improved wound angiogenesis in chronic wounds, providing evidence of probable mechanisms of action of PRFM in healing of chronic ulcers.
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Affiliation(s)
- Sashwati Roy
- Comprehensive Wound Center, Department of Surgery, Davis Heart and Lung Research Institute, The Ohio State University Medical Center, Columbus, Ohio 43210, USA
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Agnihotri A, Agnihotri D. Maxillary Sinus Lift Up: An Indirect Approach for Implant Placement in Posterior Maxilla. ACTA ACUST UNITED AC 2012. [DOI: 10.5005/jp-journals-10012-1075] [Citation(s) in RCA: 2] [Impact Index Per Article: 0.2] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 01/01/2023]
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Jain V, TarunKumar AB. Alveolar Ridge Preservation with β-TCP Graft and Platelet-Rich Fibrin. ACTA ACUST UNITED AC 2012. [DOI: 10.5005/jp-journals-10012-1074] [Citation(s) in RCA: 4] [Impact Index Per Article: 0.3] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/23/2022]
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Moghaddas H, Amjadi MR, Naghsh N. Clinical and biometrical evaluation of socket preservation using demineralized freeze-dried bone allograft with and without the palatal connective tissue as a biologic membrane. Dent Res J (Isfahan) 2012; 9:758-63. [PMID: 23559955 PMCID: PMC3612227] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 10/26/2022] Open
Abstract
BACKGROUND Alveolar ridge preservation following tooth extraction has the ability to maintain the ridge dimensions and allow the implant placement in an ideal position fulfilling both functional and aesthetic results. The aim of this study was to evaluate the efficacy of the palatal connective tissue as a biological membrane for socket preservation with demineralized freeze-dried bone allograft (DFDBA). MATERIALS AND METHODS Twelve extraction sites were treated with DFDBA with (case group) and without (control group) using autogenous palatal connective tissue membrane before placement of implants. Alveolar width and height, amount of keratinized tissue, and gingival level were measured at pre-determined points using a surgical stent at two times, the time of socket preservation surgery. RESULTS In both groups a decrease in all socket dimensions was found. The average decrease in socket width, height, keratinized tissue, and gingival level in case group was 1.16, 0.72, 3.58, and 1.27 mm, and in control group was 2.08, 0.86, 4.52, and 1.58 mm respectively. Statistical analysis showed that decrease in socket width (P = 0.012), keratinized tissue (P ≤ 0.001), and gingival level (P = 0.031) in case group was significantly lower than that of the control group. Results showed no meaningful difference in socket height changes when compared with case and control groups (P = 0.148). CONCLUSION Under the limits of this study, connective tissue membrane could preserve socket width, amount of keratinized tissue, and the gingival level more effectively than DFDBA alone.
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Affiliation(s)
- Hamid Moghaddas
- Department of Periodontology, Faculty of Dentistry, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran
| | - Mohammad Reza Amjadi
- Torabinejad Dental Research Center and Department of Periodontology, School of Dentistry, Isfahan University of Medical Sciences, Isfahan, Iran,Address for correspondence: Dr. Mohammad Reza Amjadi, Torabinejad Dental Research Center and Department of Periodontology, School of Dentistry, Isfahan University of Medical Sciences, Isfahan, Iran. E-mail:
| | - Narges Naghsh
- Dental Implant Research Center and Department of Periodontology, School of Dentistry, Isfahan University of Medical Sciences, Isfahan, Iran
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