Exercise promotes osteogenic differentiation by activating the long non-coding RNA H19/microRNA-149 axis

Table 1 Treadmill exercise program

Stages	Time	Speed	Duration
Acclimatization treadmill exercise	Day 1	6 m/min	30 min
	Day 2	9 m/min	40 min
	Day 3	12 m/min	50 min
Formal treadmill exercise	Days 4 to 7	15 m/min	60 min
	Weeks 2 to 8	15 m/min	60 min

Table 2 Primer sequences

Genes	Primer sequence (5'-3')
lncRNA H19	Forward: 5'- GCTCCACTGACCTTCTAAAC -3' Reverse: 5'- ACGATGTCTCCTTTGCTAAC -3'
miR-149	Forward: 5'- TGGCTCCGTATCTTCACTCC -3'
β-actin	Forward: 5'-CTGTCCCTGTATGCCTCTG-3' Reverse: 5'-ATGTCACGCACGATTTCC-3'
ALP	Forward: 5'-CCAACTCTTTTGTGCCAGAGA-3' Reverse: 5'-GGCTACATTGGTGTTGAGCTTTT-3'
Runx2	Forward: 5'-AGAGTCAGATTACAGATCCCAGG-3' Reverse: 5'-TGGCTCTTCTTACTGAGAGAGG-3'
BMP2	Forward: 5'- GTGCAGATCCTAGGTTTCTCTG-3' Reverse: 5’- CAGGATCTCATTCTCTTGGATC-3’
Osterix	Forward: 5'-TGGTACAAGGCAGGCATCCA-3' Reverse: 5'-GGAGCAAAGTCAGATGGGTAAGT-3'