Basic Study
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World J Orthop. Jan 18, 2025; 16(1): 101424
Published online Jan 18, 2025. doi: 10.5312/wjo.v16.i1.101424
Comparative study of a rabbit model of spinal tuberculosis using different concentrations of Mycobacterium tuberculosis
Yong-Jie Qiao, Xiao-Yang Song, Lv-Dan Zhang, Feng Li, Hao-Qiang Zhang, Sheng-Hu Zhou
Yong-Jie Qiao, Department of Joint Surgery, The Second Hospital & Clinical Medical School, Lanzhou University, Lanzhou 730050, Gansu Province, China
Yong-Jie Qiao, Xiao-Yang Song, Hao-Qiang Zhang, Sheng-Hu Zhou, Department of Orthopedics, The 940th Hospital of Joint Logistic Support Force of Chinese People’s Liberation Army, Lanzhou 730000, Gansu Province, China
Lv-Dan Zhang, Department of Respiratory Medicine, The 940th Hospital of Joint Logistic Support Force of Chinese People’s Liberation Army, Lanzhou 730050, Gansu Province, China
Feng Li, Department of Orthopaedics, The 943rd Hospital of Joint Logistic Support Force of Chinese People’s Liberation Army, Wuwei 733099, Gansu Province, China
Co-first authors: Yong-Jie Qiao and Xiao-Yang Song.
Co-corresponding authors: Sheng-Hu Zhou and Hao-Qiang Zhang.
Author contributions: Qiao YJ and Song XY contribute equally to this study as co-first authors; Zhou SH and Zhang HQ contribute equally to this study as co-corresponding authors; Qiao YJ, Song XY and Zhang LD participated in writing the manuscript; Liu P and Li F performed the data collection; Qiao YJ and Zhang LD performed the radiologic imaging analysis; Zhang HQ performed the pathological features analysis; Zhou SH participated in following postoperative effect, and helped examine and correct the manuscript; all authors have read and approved the final manuscript.
Supported by Lanzhou City Science and Technology Development Guiding Plan Project, No. 2023-ZD-170; Lanzhou Science and Technology Plan Project, No. 2023-2-11; and High-Level Talent Training Project At the 940th Hospital of the Joint Logistics Force, No. 2024-G3-5.
Institutional animal care and use committee statement: Ethical approval was obtained from the Ethics Committee of the 940th Hospital of Joint Logistic Support Force of Chinese People’s Liberation Army (ID: 2020KYLL138). Informed consent was sought from all the members before the beginning of the study.
Conflict-of-interest statement: All the authors report no relevant conflicts of interest for this article.
Data sharing statement: Data sharing statement: Participants gave informed consent for data sharing.
ARRIVE guidelines statement: The authors have read the ARRIVE guidelines, and the manuscript was prepared and revised according to the ARRIVE guidelines.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Sheng-Hu Zhou, PhD, Associate Chief Physician, Department of Orthopedics, the 940th Hospital of Joint Logistic Support Force of Chinese People’s Liberation Army, No. 333 South Riverside Road, Lanzhou 730000, Gansu Province, China. zhoushenghu120@163.com
Received: September 13, 2024
Revised: November 3, 2024
Accepted: December 19, 2024
Published online: January 18, 2025
Processing time: 121 Days and 17 Hours
Core Tip

Core Tip: One study reported having established osteoarticular tuberculosis using non-sensitized animals; however, the result of the experiment was unsatisfactory because of the high mortality rate. In recent studies, Liu and Geng successfully established a spinal tuberculosis model in New Zealand rabbits after sensitizing the rabbits with complete Freund’s adjuvant. However, they established the model without making a comparative study of the effect of using different bacterial concentrations. In this study, we established a model using three concentrations of bacteria and carried out a comprehensive evaluation of the model by imaging, general observations, and histopathological and bacteriological studies.