Basic Study
Copyright ©The Author(s) 2018. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Orthop. Oct 18, 2018; 9(10): 190-197
Published online Oct 18, 2018. doi: 10.5312/wjo.v9.i10.190
Dose of alendronate directly increases trabeculae expansivity without altering bone volume in rat femurs
Suyany G Weiss, Gabrielle O Kuchar, Jennifer T Gerber, Fernanda Tiboni, Carmen Lucia M Storrer, Thaís C Casagrande, Allan F Giovanini, Rafaela Scariot
Suyany G Weiss, Gabrielle O Kuchar, Jennifer T Gerber, Fernanda Tiboni, Carmen Lucia M Storrer, Allan F Giovanini, Rafaela Scariot, School of Health Science, Department of Dentistry, Positivo University, Curitiba, Paraná 81280-330, Brazil
Thaís C Casagrande, School of Health Science, Department of Biotecnology, Positivo University, Curitiba, Paraná 81280-330, Brazil
Author contributions: Weiss SG performed the majority of the experiments and analyzed the data; Kuchar GO, Gerber JT and Tiboni F participated in treatment of animals; Casagrande TC was involved in surgical procedures; Storrer CLM and Giovanini AF contributed to the writing of the paper; Scariot R designed the study, performed some surgical procedures and coordinated the research.
Institutional animal care and use committee statement: The Ethics Committee on the Use of Animals (ECUA 320) approved this research.
Conflict-of-interest statement: All authors have no conflicts of interest to report.
ARRIVE guidelines statement: The authors have read the ARRIVE guidelines, and the manuscript was prepared and revised according to the ARRIVE guidelines.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Correspondence to: Rafaela Scariot, PhD, Associate Professor, School of Health Science, Department of Dentistry, Positivo University, 5300 Professor Pedro Viriato Parigot de Souza Street, Campo Comprido, Curitiba, Paraná 81280-330, Brazil. rafaela.moraes@up.edu.br
Telephone: +55-41-33173454 Fax: +55-41-33173454
Received: May 7, 2018
Peer-review started: May 7, 2018
First decision: June 14, 2018
Revised: June 20, 2018
Accepted: June 28, 2018
Article in press: June 28, 2018
Published online: October 18, 2018
Processing time: 165 Days and 16.4 Hours
Abstract
AIM

To evaluate the effects of sodium alendronate on bone repair in fractures created in appendicular bones.

METHODS

Wistar rats (n = 36) were allocated into three distinct groups: group C (control), group B1 (received 1 mg/kg of alendronate), and group B2 (received 3 mg/kg of alendronate). The rats underwent femoral transversal linear fracture surgery using stable internal fixation with a 2.0 mm plate and screw system. Each animal randomly received intraperitoneal applications of sodium alendronate at a dose corresponding to group B1 or B2 three times a week, while the control group received a 0.9% saline solution. Drug administration was performed until euthanasia at 45 d. The femurs were removed and each surgical piece was sent for radiographic, tomographic and microtomographic analysis. Data were submitted to descriptive and inferential statistical analysis (95% confidence interval).

RESULTS

Quantitative evaluations of bone neoformation did not show differences among the groups in the radiographic (P = 0.341), microtomographic (P = 0.581) and tomographic evaluations (P = 0.171). In the qualitative microtomographic analysis, a smaller distance was observed between the internal bone trabeculae in the groups that used alendronate (P = 0.05). On the other hand, group B2 had a higher amount of bone trabeculae per unit length when compared to the other groups (P = 0.04).

CONCLUSION

It is likely that the use of alendronate did not have a direct influence on the amount of bone neoformation, however it did influence the bone quality in a dose-dependent manner, ultimately affecting the distance and quantity of the trabeculae.

Keywords: Alendronate; Bone regeneration; Fracture; Femur; Bisphosphonates

Core tip: Several studies have been carried out to determine both the influence of alendronate in bone repair and the appropriate dose of this drug to promote bone regeneration. In this research, 36 Wistar rats were allocated into three distinct groups that received applications of either alendronate at different doses or saline solution three times a week for 45 d. The rats underwent femoral fracture surgery with stable internal fixation. The imaginologic results suggested that the use of alendronate did not have a direct influence on the amount of bone neoformation, however it did influence bone quality in a dose-dependent manner.