Zhan D, Thumtecho S, Tanavalee A, Yuktanandana P, Anomasiri W, Honsawek S. Association of adiponectin gene polymorphisms with knee osteoarthritis. World J Orthop 2017; 8(9): 719-725 [PMID: 28979856 DOI: 10.5312/wjo.v8.i9.719]
Corresponding Author of This Article
Sittisak Honsawek, Professor, Department of Biochemistry, Faculty of Medicine, Chulalongkorn University, King Chulalongkorn Memorial Hospital, Thai Red Cross Society, 1873 Rama IV Rd, Patumwan, Bangkok 10330, Thailand. sittisak.h@chula.ac.th
Research Domain of This Article
Orthopedics
Article-Type of This Article
Prospective Study
Open-Access Policy of This Article
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Dong Zhan, Wilai Anomasiri, Sittisak Honsawek, Department of Biochemistry, Faculty of Medicine, Chulalongkorn University, King Chulalongkorn Memorial Hospital, Thai Red Cross Society, Bangkok 10330, Thailand
Suthimon Thumtecho, Faculty of Medicine, Chulalongkorn University, King Chulalongkorn Memorial Hospital, Thai Red Cross Society, Bangkok 10330, Thailand
Aree Tanavalee, Pongsak Yuktanandana, Sittisak Honsawek, Vinai Parkpian Orthopaedic Research Center, Department of Orthopaedics, Faculty of Medicine, Chulalongkorn University, King Chulalongkorn Memorial Hospital, Thai Red Cross Society, Bangkok 10330, Thailand
Author contributions: Zhan D, Anomasiri W and Honsawek S designed research; Tanavalee A and Yuktanandana P treated patients and collected samples and clinical data from patients; Zhan D and Thumtecho S performed the assays; Zhan D and Honsawek S analysed data; Zhan D and Honsawek S wrote the manuscript and revised the manuscript for final submission.
Institutional review board statement: This study was approved by the Institutional Review Board on Human Research of the Faculty of Medicine, Chulalongkorn University.
Informed consent statement: All study participants provided written informed consent prior to study enrollment.
Conflict-of-interest statement: The authors declare that they have no conflict of interest.
Data sharing statement: Technical appendix, statistical code, and dataset available from the corresponding author at sittisak.h@chula.ac.th. Participants gave informed consent for data sharing.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Correspondence to: Sittisak Honsawek, Professor, Department of Biochemistry, Faculty of Medicine, Chulalongkorn University, King Chulalongkorn Memorial Hospital, Thai Red Cross Society, 1873 Rama IV Rd, Patumwan, Bangkok 10330, Thailand. sittisak.h@chula.ac.th
Telephone: +66-22-2564482 Fax: +66-22-2564482
Received: January 30, 2017 Peer-review started: February 12, 2017 First decision: March 28, 2017 Revised: April 19, 2017 Accepted: May 12, 2017 Article in press: May 13, 2017 Published online: September 18, 2017 Processing time: 225 Days and 13.1 Hours
Abstract
AIM
To investigate the possible relationship of adiponectin (ADIPOQ) gene polymorphisms, plasma adiponectin, and the risk of knee osteoarthritis (OA).
METHODS
A total of 398 subjects, 202 knee OA patients and 196 healthy individuals, were enrolled in the case-control study. Genotyping at +45T/G (rs2241766) and +276G/T (rs1501299) loci was performed using polymerase chain reaction-restriction fragment length polymorphism. Plasma adiponectin levels were assessed using enzyme-linked immunosorbent assay. OA severity was determined using the Kellgren-Lawrence (KL) grading system.
RESULTS
No significant associations were observed in the genotype distributions and allele frequencies at two loci of +45T/G and +276G/T polymorphisms in the ADIPOQ between knee OA patients and control subjects. There was a significant association between genotype distribution of +276G/T polymorphism and KL grade 2, 3 or 4 (P = 0.037, P = 0.046, P = 0.016, respectively). At +45T/G locus, the percentage of GG genotype was notably greater in control subjects (13.40%) compared with OA subjects (1.70%) (P = 0.023). Plasma adiponectin was markedly decreased in OA subjects compared with control subjects (P = 0.03). Likewise, circulating adiponectin in OA subjects was notably lesser than that in control subjects in GG genotype of +45T/G (P = 0.029) and +276G/T polymorphisms (P = 0.012).
CONCLUSION
Polymorphisms +45T/G and +276G/T of the ADIPOQ gene might not be responsible for OA susceptibility among Thais.
Core tip: Plasma adiponectin levels were significantly lower in knee osteoarthritis (OA) than controls. No significant associations were observed in the genotype distributions and allele frequencies of ADIPOQ +45T/G and +276G/T polymorphisms between knee OA subjects and controls. There was a significant association between genotype distribution of +276G/T polymorphism and OA severity. In addition, plasma adiponectin in OA subjects was seemingly lower than that in control subjects in GG genotype of +45T/G and +276G/T polymorphisms. Polymorphisms +45T/G and +276G/T of the ADIPOQ gene might not be responsible for the susceptibility to knee OA in the Thai population.
