Banxia xiexin decoction prevents the development of gastric cancer

Table 1 Sequence list of polymerase chain reaction primers

Primer name	Upstream primer sequence (5’-3’)	Downstream primer sequences (5’-3’)
AKT1	GCTCAGCCCACCCTTCAAG	GCTGTCATCTTGGTCAGGTGGT
PIK3CA	TAGTGTCCGGGAAAATGGCT	GGCATGCTCTTCGATCACAG
EGFR	GCCAAGGCACGAGTAACAAGC	GGGCAATGAGGACATAACCAGC
BCL2	AGACGGAAGAGAAATTCACTGG	GCCATAATCTCATCAGGGG
IL6	GTAGTGAGGTTCAAG	GCTGTCATCTTGGTCAGGTGGT
GAPDH	GAAGGTCGGAGTCAACGGAT	CCTGGAAGATGGTGATGGG

Table 2 Comparison of body weight, tumor volume, tumor weight, and tumor inhibition rate of nude mouse

Group	Number	Weight/g	Tumor volume/mm3	Tumor weight/g	Anti-tumor rate (%)
Control	3	18.83 ± 0.15
Model	3	15.133 ± 0.40b	1641.48 ± 499.84	1.25 ± 0.13
Capecitabine	3	17.633 ± 0.06b	616.98 ± 156.00a	0.56 ± 0.15b	62.41
BXD-L	3	16.833 ± 0.06b	726.37 ± 168.48a	0.77 ± 0.08b	55.75
BXD-M	3	17.673 ± 0.15b	541.85 ± 95.72a	0.51 ± 0.09b	66.99
BXD-H	3	17 ± 0.1b	682.00 ± 281.61a	0.55 ± 0.13b	58.48

^aP < 0.05.

^bP < 0.01.

BXD-L: Banxia xiexin decoction low dose; BXD-M: Banxia Xiexin decoction medium dose; BXD-H: Banxia xiexin decoction high dose.

Table 3 IC₅₀ screening and cell proliferation assay of Cell Counting Kit-8 for the detection of banxia xiexin decoction in MGC803

Group	24 hours OD	Proliferation rate (%)	48 hours OD	Proliferation rate (%)
Control	1.88 ± 0.02		2.73 ± 0.04
10% BXD	1.85 ± 0.01	98	2.36 ± 0.06b	86.09
20% BXD	0.91 ± 0.07b	45.78	1.53 ± 0.02b	54.62
40% BXD	0.48 ± 0.01b	21.18	0.54 ± 0.02b	17.09
Capecitabine	1.00 ± 0.06b	50.48	1.65 ± 0.19b	59.11
LY294002	1.00 ± 0.01b	50.25	1.00 ± 0.07b	34.7
BXD + LY294002	0.64 ± 0.04b	30.07	0.77 ± 0.05b	26.05

^bP < 0.01.

BXD-L: Banxia xiexin decoction low dose; BXD-M: Banxia Xiexin decoction medium dose; BXD-H: Banxia xiexin decoction high dose; OD: Optical density.

Table 4 mRNA expression content in the phosphoinositide 3-kinase/protein kinase B signaling pathway (mean ± SD)

Group	EGFR	PIK3CA	AKT1	IL6	BCL2
Model	1.00 ± 0.1	0.80 ± 0.25	1.01 ± 0.11	1.56 ± 0.06	2.23 ± 0.52
Capecitabine	0.97 ± 0.4	0.34 ± 0.17a	0.07 ± 0.04b	1.01 ± 0.14b	0.52 ± 0.13b
BXD	0.79 ± 0.39a	0.41 ± 0.18	0.04 ± 0.01b	1.52 ± 0.25	1.09 ± 0.56a
LY294002	0.25 ± 0.14b	0.07 ± 0.02b	0.09 ± 0.05b	1.59 ± 0.17	0.85 ± 0.47a
BXD + LY294002	0.01 ± 0.01b	0.38 ± 0.21	0.06 ± 0.01b	0.74 ± 0.07b	0.40 ± 0.11b

^aP < 0.05.

^bP < 0.01.

BXD: Banxia xiexin decoction.