Banxia xiexin decoction prevents the development of gastric cancer

Figure 1 Experimental flow chart. BXD: Banxia xiexin decoction; UPLC-Q-Orbitrap: Ultra-performance liquid chromatography tandem quadrupole electrostatic field orbitrap; DEGs: Differentially expressed genes; GES: Gastric epithelial cells; PPI: Protein-protein interaction; GO: Gene ontology; KEGG: Kyoto encyclopedia of genes and genomes; HE: Hematoxylin-eosin; BXD-L: Banxia xiexin decoction low dose; BXD-M: Banxia xiexin decoction medium dose; BXD-H: Banxia xiexin decoction high dose; RT-PCR: Reverse transcription polymerase chain reaction.

Figure 2 Total ion flow mapping for banxia xiexin decoction natural product identification. A: Black, shows the total ion flow diagram for the negative ion mode; B: Red, shows the total ion flow diagram for the positive ion mode.

Figure 3 Hematoxylin-eosin staining of tumor tissues in each group. BXD-L: Banxia xiexin decoction low dose; BXD-M: Banxia Xiexin decoction medium dose; BXD-H: Banxia xiexin decoction high dose.

Figure 4  Protein-protein interaction network of differentially expressed genes.

Figure 5 Functional enrichment analysis of differentially expressed genes. A: Gene ontology analysis showing biological processes, cellular components and molecular functions involved in differentially expressed genes; B: Kyoto encyclopedia of genes and genomes showing signaling pathways involved in differentially expressed genes. BP: Biological process; CC: Cellular component; MF: Molecular function.

Figure 6 Effect of different administration groups on apoptosis of gastric cancer cells. ^bP < 0.01. BXD: Banxia xiexin decoction.

Figure 7 Effect of different administration groups on gastric cancer cell migration. BXD: Banxia xiexin decoction.

Figure 8 Effect of different administration groups on mitochondrial membrane potential of gastric cancer cells. ^bP < 0.01. BXD: Banxia xiexin decoction.