Gastric cancer molecular classification based on immunohistochemistry and in situ hybridization: Analysis in western patients after curative-intent surgery

Figure 1 Results for Epstein-Barr virus infection by in situ hybridization, and for microsatellite instability, e-cadherin and p53 expression by immunohistochemistry. EBV: Epstein-Barr virus; MSI: Microsatellite instability.

Figure 2 Microscopic findings in gastric cancer cases. A: Tumor positive for Epstein-Barr virus (EBV) by in situ hybridization; B: Gastric cancer (GC) negative for EBV infection; C: Imuno-histoquímico (IHC) analysis of MLH1 expression in tumors with retained expression of MLH1; D: Loss of MLH1 expression; E: GC with preserved E-cadherin expression; F: tumor exhibiting loss of E-cadherin expression; G: Normal IHC expression of p53; H: tumor with aberrant p53 expression.

Figure 3 Flowchart showing the classification of molecular subtypes and final distribution. A: Classification by TGCG; B: Asian Cancer Research Group classification. EBV: Epstein-Barr virus; EMT: Epithelial to mesenchymal transition; GS: Genomically stable; ISH: In situ hybridization; MSI: Microsatellite instability; MSS: Microsatellite stable.

Figure 4 Disease-free survival and overall survival according to the Cancer Genome Atlas and Asian Cancer Research Group classification. A: Disease-free survival according to the Cancer Genome Atlas (TCGA) classification; B: Overall survival according to TCGA classification; C: Disease-free survival according to Asian Cancer Research Group (ACRG) classification; D: Overall survival according to ACRG classification. CIN: Chromosomal instability; EBV: Epstein-Barr virus; EMT: Epithelial to mesenchymal transition; GS: Genomically stable; MSI: Microsatellite instability; MSS: Microsatellite stable.