Retrospective Study
Copyright ©The Author(s) 2021. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Clin Oncol. Aug 24, 2021; 12(8): 688-701
Published online Aug 24, 2021. doi: 10.5306/wjco.v12.i8.688
Gastric cancer molecular classification based on immunohistochemistry and in situ hybridization: Analysis in western patients after curative-intent surgery
Marcus Fernando Kodama Pertille Ramos, Marina Alessandra Pereira, Evandro Sobroza de Mello, Cinthya dos Santos Cirqueira, Bruno Zilberstein, Venancio Avancini Ferreira Alves, Ulysses Ribeiro-Junior, Ivan Cecconello
Marcus Fernando Kodama Pertille Ramos, Marina Alessandra Pereira, Bruno Zilberstein, Ulysses Ribeiro-Junior, Ivan Cecconello, Department of Gastroenterology, Instituto do Cancer, Hospital das Clinicas, HCFMUSP, Faculdade de Medicina, Universidade de São Paulo, São Paulo 01249000, Brazil
Evandro Sobroza de Mello, Venancio Avancini Ferreira Alves, Department of Pathology, Instituto do Cancer, Hospital das Clinicas HCFMUSP, Faculdade de Medicina, Universidade de Sao Paulo, São Paulo 01249000, Brazil
Cinthya dos Santos Cirqueira, Department of Pathology, Instituto Adolfo Lutz, São Paulo 01246000, Brazil
Author contributions: Ramos MFKP, Pereira MA and Ribeiro-Junior U performed the study design. Ramos MFKP and Pereira MA data retrieval, critical analysis, and draft of the manuscript; De Mello ES, Cirqueira CDS, and Alves VAF performed the laboratory techniques and pathological analysis; Ribeiro-Junior U, Zilberstein B, and Cecconello I performed the critical analysis and review of the manuscript.
Supported by Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP agency), No. 2016/25524-0.
Institutional review board statement: The study was approved by the hospital ethics committee and registered online (https://plataformabrasil.saude.gov.br), No. CAAE: 37009120.0.0000.0068.
Informed consent statement: Informed consent was waived by the local Ethics Committee because of the retrospective nature of the study.
Conflict-of-interest statement: The authors declare that they have no conflicts of interest that might be relevant to the contents of this manuscript.
Data sharing statement: No additional data are available.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Marina Alessandra Pereira, MSc, Research Scientist, Department of Gastroenterology, Instituto do Cancer, Hospital das Clinicas, HCFMUSP, Faculdade de Medicina, Universidade de São Paulo, Av Dr Arnaldo 251, São Paulo 01249000, Brazil. marina.pereira@hc.fm.usp.br
Received: January 27, 2021
Peer-review started: January 27, 2021
First decision: March 31, 2021
Revised: April 9, 2021
Accepted: July 23, 2021
Article in press: July 23, 2021
Published online: August 24, 2021
Processing time: 208 Days and 1.7 Hours
Core Tip

Core Tip: In this study, patients with gastric cancer (GC) were retrospectively evaluated based on subgroups of molecular classification by immunohistochemistry and in situ hybridization. Microsatellite instability status, e-cadherin, p53 expression, and Epstein-Barr virus were evaluated in a Western cohort of GC patients treated with curative intent, where it was possible to obtain subgroups with different clinicopathological characteristics and prognosis. Thus, our findings demonstrate that through techniques used in the routine pathological evaluation it is possible to identify immunophenotypic groups of GC similar to those determined by the molecular classification.