Basic Study
Copyright ©The Author(s) 2020. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastrointest Pharmacol Ther. Nov 8, 2020; 11(5): 93-109
Published online Nov 8, 2020. doi: 10.4292/wjgpt.v11.i5.93
Bowel adhesion and therapy with the stable gastric pentadecapeptide BPC 157, L-NAME and L-arginine in rats
Lidija Berkopic Cesar, Slaven Gojkovic, Ivan Krezic, Dominik Malekinusic, Helena Zizek, Lovorka Batelja Vuletic, Andreja Petrovic, Katarina Horvat Pavlov, Domagoj Drmic, Antonio Kokot, Josipa Vlainic, Sven Seiwerth, Predrag Sikiric
Lidija Berkopic Cesar, Slaven Gojkovic, Ivan Krezic, Dominik Malekinusic, Helena Zizek, Antonio Kokot, Predrag Sikiric, Department of Pharmacology, School of Medicine, University of Zagreb, Zagreb 10 000, Croatia
Lovorka Batelja Vuletic, Andreja Petrovic, Katarina Horvat Pavlov, Sven Seiwerth, Department of Pathology, School of Medicine, University of Zagreb, Zagreb 10000, Croatia
Josipa Vlainic, Institute Ruder Boskovic, Zagreb 10000, Croatia
Author contributions: Berkopic Cesar L was involved in conceptualization and methodology; Gojkovic S took part in the visualization and investigation; Krezic I took part in the visualization and investigation; Malekinusic D provided study resources; Zizek H was involved in visualization and investigation; Batelja Vuletic L took part in the formal analysis; Petrovic A was involved in formal analysis; Horvat Pavlov K took part in the formal analysis; Drmic D provided study resources; Kokot A took part in the validation; Vlainic J was involved in conceptualization and methodology; Seiwerth S provided writing, review and editing of the manuscript; Sikiric P provided writing, review and editing of the manuscript.
Institutional animal care and use committee statement: This research was approved by local Ethic Committee (case number 380-59-10106-17-100/290) and by Directorate of Veterinary (UP/I-322-01/15-01/22).
Conflict-of-interest statement: The authors state that they have no conflicts of interest.
Data sharing statement: The data that support the findings of this study are available on request from the corresponding author.
ARRIVE guidelines statement: The authors have read the ARRIVE guidelines, and the manuscript was prepared and revised according to the ARRIVE guidelines.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Predrag Sikiric, MD, PhD, Professor, Department of Pharmacology, School of Medicine, University of Zagreb, Salata 11, Zagreb 10000, Croatia. sikiric@mef.hr
Received: February 13, 2020
Peer-review started: February 13, 2020
First decision: March 26, 2020
Revised: August 13, 2020
Accepted: September 10, 2020
Article in press: September 10, 2020
Published online: November 8, 2020
Processing time: 266 Days and 12.2 Hours
Core Tip

Core Tip: After parietal peritoneum excision with an underlying superficial layer of muscle tissue in rats, there is failed vasculature, and finally, increased adhesion formation. Unlike nitric oxide-agents, L-NAME and/or L-arginine diverse effects, the application of the stable gastric pentadecapeptide BPC 157 with its most recent described vascular effects (“vascular recruitment”) recovers abundant vascular vessel presentation in and close to the defect, which occurs rapidly. Finally, BPC 157 attenuated bowel adhesion formation and nitric oxide- and MDA-tissue values. Thus, BPC 157 therapy can be suited for the realization of the peritoneal defect healing with minimal or no adhesion formation.