Ikaros in B cell development and function

Table 1 Ikaros co-factors

Protein/complex	Citation	System
NuRD complex (Mi2b, HDACs)	[31]	Over-expression in murine T cells
	[30]	Over-expression in a murine DP thymocyte cell line
	[41]	Endogenous proteins in a murine erythro-leukemia cell line
	[30]	Over-expression in non-lymphoid cell lines
Sin3 complex (Sin3a/b, HDACs)	[34]	Over-expression in non-lymphoid cell lines and in murine T cells
	[32]	Over-expression in non-lymphoid cell lines
CtBP	[34]	Over-expression in non-lymphoid cell lines and in murine T cells
	[32]	Over-expression in non-lymphoid cell lines
CtIP	[35]	Over-expression in non-lymphoid cell lines and in murine T cells
SWI/SNF complex (BRG1, BAFs)	[31]	Over-expression in murine T cells
	[41]	Endogenous proteins in a murine erythro-leukemia cell line
pTEFb (cdk9)	[43]	Endogenous proteins in murine yolk sac erythroid cells

CtBP: C terminal binding protein; CtIP: CtBP interacting protein; pTEFb: Positive transcriptional elongation factor complex; HDAC: Histone deacetylase.

Table 2 Critical members of the transcription factor network controlling B cell development

Gene	Ref.	Roles
Ikaros (Ikzf1)	[64]	Required for earliest B cell progenitors
	[66,67]	Required for efficient pro-B to pre-B transition
	[67]	Required for repression of non-B cell fates
	[65,67]	Promotes Igh rearrangment and Rag expression
E2A (Tcfe2a)	[51]	Required for earliest B cell progenitors
	[86]	Required for EBF1 expression
EBF1 (Ebf1)	[87]	Required for expression of B lineage genes development past pro-B stage
Pax5 (Pax5)	[56]	Required for differentiation past pro-B stage
	[57,58]	Required for commitment to B cell lineage (e.g. repression of alternative fates)

EBF1: Early B cell factor 1.