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World J Biol Chem. Nov 26, 2014; 5(4): 437-456
Published online Nov 26, 2014. doi: 10.4331/wjbc.v5.i4.437
Published online Nov 26, 2014. doi: 10.4331/wjbc.v5.i4.437
Identification of host miRNAs that may limit human rhinovirus replication
Victor Paky Bondanese, LGL, LabEx LIO, ENS Lyon, Site Monod, 69364 Lyon, France
Victor Paky Bondanese, Ana Francisco-Garcia, Nicole Bedke, Donna E Davies, Tilman Sanchez-Elsner, Academic Unit of Clinical and Experimental Sciences, University of Southampton Faculty of Medicine, University Hospital Southampton, Southampton SO16 6YD, United Kingdom
Nicole Bedke, Immunocore Limited, Milton Park, Abingdon, Oxon OX14 4RY, United Kingdom
Author contributions: Bondanese VP and Francisco-Garcia A contributed equally to this work, they performed all the experiments and analyzed the data; Bedke N provided essential reagents; Davies ED and Sanchez-Elsner T conceived the study; Bondanese VP, Davies ED and Sanchez-Elsner T designed the experiments and wrote the manuscript; all authors contributed to the interpretation of the data, revised the manuscript and approved its final form.
Supported by MRC, AAIR and the Roger Brooke charitable trust
Correspondence to: Dr. Tilman Sanchez-Elsner, PhD, Senior Lecturer in Biomedical Sciences, Academic Unit of Clinical and Experimental Sciences, University of Southampton, Faculty of Medicine, Southampton General Hospital, Tremona Road, Southampton SO16 6YD, United Kingdom. t.sanchez-elsner@soton.ac.uk
Telephone: +44-23-80794410 Fax: +44-23-81201761
Received: May 29, 2014
Revised: September 9, 2014
Accepted: October 1, 2014
Published online: November 26, 2014
Processing time: 187 Days and 3.6 Hours
Revised: September 9, 2014
Accepted: October 1, 2014
Published online: November 26, 2014
Processing time: 187 Days and 3.6 Hours
Core Tip
Core tip: Our results show for the first time that: (1) DICER knock-down increases HRV-1B replication in human bronchial epithelial cells; (2) the genomic RNA of human rhinovirus (HRV)-1B interacts directly with the miRISC during infection; and (3) inhibition of two microRNAs predicted to target HRV-1B, i.e., miR-128 and miR-155, favors viral replication. This supports a role for cellular microRNAs in the antiviral response to HRV-1B mounted by bronchial epithelial cells, and suggests that pathological microRNA dysregulation may contribute to the poor antiviral immunity in diseases such as asthma and chronic obstructive pulmonary disease.