Galliher-Beckley AJ, Lan LQ, Aono S, Wang L, Shi J. Caspase-1 activation and mature interleukin-1β release are uncoupled events in monocytes. World J Biol Chem 2013; 4(2): 30-34 [PMID: 23710297 DOI: 10.4331/wjbc.v4.i2.30]
Corresponding Author of This Article
Dr. Jishu Shi, Department of Anatomy and Physiology, College of Veterinary Medicine, Kansas State University, 119 Anderson Hall, Manhattan, KS 66506, United States. jshi@ksu.edu
Research Domain of This Article
Cell Biology
Article-Type of This Article
Brief Article
Open-Access Policy of This Article
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World J Biol Chem. May 26, 2013; 4(2): 30-34 Published online May 26, 2013. doi: 10.4331/wjbc.v4.i2.30
Caspase-1 activation and mature interleukin-1β release are uncoupled events in monocytes
Amy J Galliher-Beckley, Li-Qiong Lan, Shelly Aono, Lei Wang, Jishu Shi
Amy J Galliher-Beckley, Li-Qiong Lan, Lei Wang, Jishu Shi, Department of Anatomy and Physiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506, United States
Li-Qiong Lan, College of Life Sciences, Sichuan University, Chengdu 610065, Sichuan Province, China
Shelly Aono, Department of Anatomy, Physiology, and Pharmacology, College of Veterinary Medicine, Auburn University, Auburn, AL 36849, United States
Author contributions: Galliher-Beckley AJ and Lan LQ contribute equally, Galliher-Beckley AJ conceived experiments, analyzed data, and wrote the manuscript; Aono S conceived and performed experiments, analyzed data, and helped write the manuscript; Lan LQ conceived and performed experiments, analyzed data; Wang L and Shi J conceived experiments, analyzed data, and wrote the manuscript.
Supported by NIH R21 AI085416 (to Shi J) and NIH NCRR P20-RR017686 (to PI: Daniel Marcus; Shi J)
Correspondence to: Dr. Jishu Shi, Department of Anatomy and Physiology, College of Veterinary Medicine, Kansas State University, 119 Anderson Hall, Manhattan, KS 66506, United States. jshi@ksu.edu
Telephone: +1-785-5324506 Fax: +1-785-5324557
Received: February 13, 2013 Revised: March 26, 2013 Accepted: April 10, 2013 Published online: May 26, 2013 Processing time: 86 Days and 13 Hours
Core Tip
Core tip: Activated macrophages release large amounts of interleukin-1β (IL-1β) and macrophages deficient in caspase-1 expression have undetectable IL-1β secretion. This suggests that IL-1β release and caspase-1 activation are closely related events. We found that human α-defensin 5 (HD-5) inhibited the release of IL-1β, but not the processing of pro-IL-1β to IL-1β in lipopolysaccharide-activated monocytes stimulated with Adenosine triphosphate or nigericin. Different from IL-1β processing and release, the activation and release of caspase-1 from stimulated monocytes was not blocked by HD-5. These data suggest that caspase-1 activation/processing of pro-IL-1β by caspase-1 and the release of mature IL-1β from human monocytes are distinct and separable events.
