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©The Author(s) 2021. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Biol Chem. Jan 27, 2021; 12(1): 1-14
Published online Jan 27, 2021. doi: 10.4331/wjbc.v12.i1.1
Published online Jan 27, 2021. doi: 10.4331/wjbc.v12.i1.1
Inhibition of matrix metalloproteinase-9 secretion by dimethyl sulfoxide and cyclic adenosine monophosphate in human monocytes
Darcy R Denner, Maria LD Udan-Johns, Michael R Nichols, Department of Chemistry and Biochemistry, University of Missouri-St. Louis, St Louis, MO 63121, United States
Author contributions: Nichols MR conceived the ideas and supervised the project; Denner DR and Udan-Johns ML planned and carried out the experiments; Nichols MR wrote the manuscript in consultation with Denner DR and Udan-Johns ML. All authors have read and approve the final manuscript.
Supported by The University of Missouri-St. Louis , Alzheimer’s Association, No. NIRG-06-27267; and the Missouri Alzheimer’s and Related Disorders Research Program.
Institutional review board statement: The THP-1 human monocytic cell line was acquired commercially from American Type Culture Collection, thus no human subjects were used in this study.
Conflict-of-interest statement: There are no conflicts of interest to declare by any of the authors.
Data sharing statement: No additional data are available.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Michael R Nichols, PhD, Professor, Department of Chemistry and Biochemistry, University of Missouri-St. Louis, One University Blvd, St Louis, MO 63121, United States. nicholsmic@umsl.edu
Received: June 24, 2020
Peer-review started: June 24, 2020
First decision: November 4, 2020
Revised: November 17, 2020
Accepted: December 30, 2020
Article in press: December 30, 2020
Published online: January 27, 2021
Peer-review started: June 24, 2020
First decision: November 4, 2020
Revised: November 17, 2020
Accepted: December 30, 2020
Article in press: December 30, 2020
Published online: January 27, 2021
Core Tip
Core Tip: This article describes the stimulation of matrix metalloproteinase-9 (MMP-9) and tumor necrosis factor α (TNFα) secretion from human monocyte cells by lipopolysaccharide, and the inhibition of this process by dimethyl sulfoxide and increased intracellular levels of 3',5'-cyclic adenosine monophosphate. The experimental findings demonstrated that dimethyl sulfoxide concentration must be carefully controlled in inflammatory studies, that MMP-9 secretion occurred downstream from TNFα secretion and that the two processes were distinct, and that TNFα is an integral component of the pathway leading to MMP-9 secretion.