Calabrò E, Condello S, Currò M, Ferlazzo N, Caccamo D, Magazù S, Ientile R. Modulation of heat shock protein response in SH-SY5Y by mobile phone microwaves. World J Biol Chem 2012; 3(2): 34-40 [PMID: 22371824 DOI: 10.4331/wjbc.v3.i2.34]
Corresponding Author of This Article
Dr. Emanuele Calabrò, Department of Physics, University of Messina, Viale D’Alcontres, 31, 98166 Messina, Italy. e.calabro@yahoo.com
Article-Type of This Article
Brief Article
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Emanuele Calabrò, Salvatore Magazù, Department of Physics, University of Messina, 98166 Messina, Italy
Salvatore Condello, Monica Currò, Nadia Ferlazzo, Daniela Caccamo, Riccardo Ientile, Department of Biochemical, Physiological and Nutritional Sciences, University of Messina, 98125 Messina, Italy
Author contributions: Calabrò E designed the study together with Ientile R, participated in its coordination, and drafted the manuscript, with contributions from Condello S and Ferlazzo N; Condello S, Caccamo D and Currò M performed cell culture experiments, and carried out biochemical assays; Calabrò E also developed the electromagnetic field approach; Ientile R and Magazù S were the coordinators of the study, and gave the final approval of the version to be published.
Correspondence to: Dr. Emanuele Calabrò, Department of Physics, University of Messina, Viale D’Alcontres, 31, 98166 Messina, Italy. e.calabro@yahoo.com
Telephone: +39-90-6765019 Fax: +39-90-395344
Received: August 25, 2011 Revised: September 19, 2011 Accepted: September 26, 2011 Published online: February 26, 2012
Abstract
AIM: To investigate putative biological damage caused by GSM mobile phone frequencies by assessing electromagnetic fields during mobile phone working.
METHODS: Neuron-like cells, obtained by retinoic-acid-induced differentiation of human neuroblastoma SH-SY5Y cells, were exposed for 2 h and 4 h to microwaves at 1800 MHz frequency bands.
RESULTS: Cell stress response was evaluated by MTT assay as well as changes in the heat shock protein expression (Hsp20, Hsp27 and Hsp70) and caspase-3 activity levels, as biomarkers of apoptotic pathway. Under our experimental conditions, neither cell viability nor Hsp27 expression nor caspase-3 activity was significantly changed. Interestingly, a significant decrease in Hsp20 expression was observed at both times of exposure, whereas Hsp70 levels were significantly increased only after 4 h exposure.
CONCLUSION: The modulation of the expression of Hsps in neuronal cells can be an early response to radiofrequency microwaves.
