• Antioxidant defense
• Energy metabolism
• Freshwater
• Metabolic biomarkers
• Thermal stress

• brain protein
• cognitive function
• cordycepin
• diabetes mellitus
• free radical

• Animals
• Antioxidants
• Brain
• Chromatography, Liquid/veterinary
• Deoxyadenosines
• Diabetes Mellitus, Experimental/drug therapy
• Mice
• Oxidative Stress
• Streptozocin
• Superoxide Dismutase
• Tandem Mass Spectrometry/veterinary

The shortage of natural resources, prices, and high demand for fish oil has encouraged the use of non-traditional ingredients in aquafeed. The search for an alternative lipid source in aquafeeds has seen terrestrial vegetable oils at the epicenter of various flagship aqua-feed research. Herein, we investigated the effects of substituting fish oil (FO) with camelina oil (CO) on growth performance, fatty acid profile, digestibility, liver histology, and antioxidative status of red seabream (Pagrus major). After 56 days of the feeding trial, the results suggested that FO can be replaced with CO in the feeds of farmed red seabream without compromising growth, blood chemistry, digestibility, and overall health status.

A 56-day feeding trial to evaluate the responses of red seabream (initial weight: 1.8 ± 0.02 g) to the substitution of fish oil (FO) with camelina oil (CO) at different ratios was conducted. The control diet formulated at 46% CP (6F0C) contained only FO without CO; from the second to the fifth diet, the FO was substituted with CO at rates of 5:1 (5F1C), 4:2 (4F2C), 3:3 (3F3C), 2:4 (2F4C), and 0:6 (0F6C). The results of the present study showed that up to full substitution of FO with CO showed no significant effect on growth variables BW = 26.2 g–28.3 g), body weight gain (BWG = 1275.5–1365.3%), specific growth rate (SGR = 4.6–4.7), feed intake (FI = 25.6–27.8), feed conversion ratio (FCR = 1.0–1.1), biometric indices condition factor (CF = 2.2–2.4), hepatosomatic index (HSI = 0.9–1.1), viscerasomatic index (VSI = 7.5–9.5), and survival rates (SR = 82.2–100) with different FO substitution levels with CO. Similarly, there were no significant differences (p < 0.05) found in the whole-body composition except for the crude lipid content, and the highest value was observed in the control group (291 g/kg) compared to the other groups FO5CO1 (232 k/kg), FO4CO2 (212 g/kg), FO2CO4 (232 g/kg) and FO0CO6 (244 g/kg). Blood chemistry levels were not influenced in response to test diets: hematocrit (36–33%), glucose (Glu = 78.3–71.3 mg/dL), total protein (T-pro = 3.1–3.8 g/dL), total cholesterol (T-Chol = 196.0–241 mg/dL), blood urea nitrogen (BUN = 9.0–14.6 mg/dL), total bilirubin (T-Bil = 0.4–0.5 mg/dL), triglyceride (TG = 393.3–497.6 mg/dL), alanine aminotransferase test (ALT = 50–65.5 UL/L), aspartate aminotransferase test (AST = 38–69.3 UL/L). A remarkable modulation was observed in catalase (CAT) and superoxide dismutase (SOD) activities in the liver, as CAT and SOD values were lower with the complete FO substitution with CO (0F6C), and the highest values were observed in the control and (4F2C). This study indicates that red seabream may have the ability to maintain LC-PUFAs between tissues and diets, and CO substitution of FO could improve both lipid metabolism and oxidation resistance as well as maintain digestibility. In conclusion, dietary FO can be replaced up to 100% or 95% by CO in the diets of red seabream as long as n-3 HUFA, EPA, and DHA are incorporated at the recommended level.

• camelina oil
• liver histology
• oxidative status
• red seabream

To understand the intestinal microbial diversity and community structure of bighead carp (Aristichthys nobilis) under different feeding strategies, 39 fish from three groups (A: 9 fish, natural live food only; B: 15 fish, natural live food + fish formulated feeds; C: 15 fish, natural live food + fish formulated feed + lactic acid bacteria) were obtained for the high throughput 16S rRNA gene sequencing. We first examined five non-specific immunity indications of the carp—lysozyme (LZM), catalase (CAT), glutathione reductase (GR), glutathione peroxidase (GSH-PX), and superoxide dismutase (SOD). Interestingly, the composition of gut microbiota and related non-specific immune indices were affected by the feeding treatment of the bighead carp. Notably, all enzyme activity indexes were significantly different (p < 0.01) in the spleen and three enzyme activity indexes (LZM, GSH-PX, and SOD) had significant differences in the hepatopancreas (p < 0.001) of the carp from the three groups. The 16S rRNA gene sequencing showed higher diversity in groups B and C. Compared to group A, the relative abundance of Actinobacteria increased significantly and the relative abundance of Proteobacteria and Firmicutes decreased significantly in groups B and C at the phylum level. Functional analysis revealed the association between non-specific immune indicators and import genera in the hepatopancreas and spleen of bighead carp. This study provides new insights into the gut microbiomes and non-specific immune of bighead carp.

• 16S rRNA gene
• Aristichthys nobilis
• Lactic acid bacteria
• non-specific immunity

• antioxidant
• aspirin
• gastric lesion
• shilajit

Totoaba, Totoaba macdonaldi, is an endemic species of the Gulf of California, where wide variations in sea temperature throughout the year, surface salinities that gradually increase towards the north, and contamination by discharge of wastewater have been recorded. In addition to the challenges of reproduction and swimming, its characteristic biannual migration presents totoaba with changes in environmental factors that could affect oxidative stress indicators. The objective of this study was to assess spatial and seasonal changes in the oxidative stress indicators in muscle samples of totoaba.

Reactive oxygen species production, antioxidant enzyme activities and lipid peroxidation levels were quantified by spectrophotometry.

Results suggest spatial-temporal variations of the oxidative stress indicators in muscle of totoaba that may be associated to a complex interaction between environmental and biological factors, including reproduction and nutrient availability. These results contribute to explain the appeal of totoaba as a marketable meat and suggest totoaba may provide antioxidant nutrients to consumers.

• Antioxidants
• Fish
• Nutrition
• Peroxidation
• Totoaba

Systemic inflammation results in physiological changes, largely mediated by inflammatory cytokines. The present investigation was performed to determine the effect of Rhododendron arboreum (RAP) on inflammatory parameters in the animal model. The RAP (100 and 200 mg/kg) were pre-treated for animals, given orally for one week, followed by lipopolysaccharide (LPS) injection. Body temperature, burrowing, and open field behavioral changes were assessed. Biochemical parameters (AST, ALT, LDH, BIL, CK, Cr, BUN, and albumin) were done in the plasma after 6 h of LPS challenge. Oxidative stress markers SOD, CAT, and MDA were measured in different organs. Levels of inflammatory markers like tumor necrosis factor-alpha (TNF-α), interleukin-1-beta (IL-1β) and, interleukin-6 (IL-6) as well as VEGF, a specific sepsis marker in plasma, were quantified. The plasma enzymes, antioxidant markers and plasma pro-inflammatory cytokines were significantly restored (p < 0.5) by RAP treatment, thus preventing the multi-organ and tissue damage in LPS induced rats. The protective effect of RAP may be due to its potent antioxidant potential. Thus, RAP can prevent LPS induced oxidative stress, as well as inflammatory and multi-organ damage as reported in histopathological studies in rats when administered to the LPS treated animals. These findings indicate that RAP can benefit in the management of systemic inflammation from LPS and may have implications for a new treatment or preventive therapeutic strategies with an inflammatory component.

• Rhododendron arboreum polysaccharides
• animal model
• lipopolysaccharide
• multi-biomarker approach
• oxidative stress

• Body condition
• Digenean
• Fish
• Fish parasite
• Oxidative stress biomarkers
• Water quality

In this study, juvenile Manchurian trout, Brachymystax lenok (initial weight: 6.43 ± 0.02 g, mean ± SE) were received for nine weeks with five types of diets prepared by gradually replacing the proportion of fish oil (FO) with linseed oil (LO) from 0% (LO0) to 25% (LO25), 50% (LO50), 75% (LO75), and 100% (LO100). The eicosapentaenoic (EPA) and docosahexaenoic (DHA) composition decreased with increasing inclusion level of LO (P < 0.05). With increasing LO inclusion level, triglyceride (TAG) content of serum increased significantly, however, there was a decrease in high-density lipoprotein cholesterol (HDL) (P < 0.05). LO substitution of FO up-regulated the gene expression level of lipid metabolism-related genes Fatty Acid Desaturases 6 (FAD6), Acetyl-Coa Carboxylase (ACCα), Sterol Regulatory Element Binding Protein 1 (SREBP-1), and Sterol O- Acyl Transferase 2 (SOAT2), and down-regulated the gene expression level of Peroxisome Proliferator-Activated Receptor a (PPARα) (P < 0.05). The SOD activities of both serum and liver in LO100 were significantly lower than in LO25 (P < 0.05). The CAT activity of the liver in LO100 was significantly lower than in LO0 and LO25 (P < 0.05). This study indicates that the Manchurian trout may have the ability to synthesize LC-PUFAs from ALA, and an appropriate LO in substitution of FO (<75%) could improve both the lipid metabolism and the oxidation resistance.

• Exfoliated deciduous teeth stem cells
• Fatty acid composition
• Lipid peroxidation
• N-acetyl-L-cysteine

• Acetylcysteine/pharmacology
• Antioxidants/metabolism
• Cells, Cultured
• Child
• Dental Pulp/cytology
• Fatty Acids/metabolism
• Humans
• In Vitro Techniques
• Lipid Peroxidation
• Oxidative Stress/drug effects
• Stem Cells/drug effects

• III41030 Ministry od Education, Science and Technological development Republic of Serbia
• OI175061 Ministry of Education, Science and technological development Republic of Serbia

• Arsenic
• Arsenicosis
• Giant mine
• Small mammals
• Snowshoe hares

• Animals
• Antioxidants/metabolism
• Arsenic Poisoning/veterinary
• Breeding
• Cadmium/adverse effects
• Environmental Exposure/adverse effects
• Hares
• Liver/pathology
• Mining
• Northwest Territories
• Oxidative Stress

• Antioxidant defense
• Hibernation
• Nanorana parkeri
• Oxidative damage
• Oxidative stress

• Animals
• Antibody Formation
• Chiroptera/immunology
• Chiroptera/physiology
• Immunocompetence
• Oxidation-Reduction
• Oxidative Stress
• Principal Component Analysis
• Seasons
• Stress, Physiological
• Weather

• Consejo Nacional de Ciencia y Tecnología (CONACyT)
• Fronteras en la Ciencia

• Infectious disease
• Myxozoan endoparasite
• Oxidative stress
• PKD
• Salmonids

• Histopathology
• Kidney
• Liver
• Oxidative stress
• Sepia officinalis
• Sepsis

• Animals
• Antioxidants/pharmacology
• Cecum/pathology
• Disease Models, Animal
• Enzyme Activation
• Kidney/pathology
• Lipid Peroxidation
• Liver/pathology
• Liver Function Tests
• Male
• Oxidative Stress
• Rats
• Rats, Wistar
• Sepia/chemistry
• Sepsis/pathology

The world is projected to experience an approximate doubling of atmospheric CO₂ concentration in the next decades. Rise in atmospheric CO₂ level as one of the most important reasons is expected to contribute to raise the mean global temperature 1.4 °C-5.8 °C by that time. A survey from 128 countries speculates that global warming is primarily due to increase in atmospheric CO₂ level that is produced mainly by anthropogenic activities. Exposure of animals to high environmental temperatures is mostly accompanied by unwanted acceleration of certain biochemical pathways in their cells. One of such examples is augmentation in generation of reactive oxygen species (ROS) and subsequent increase in oxidation of lipids, proteins and nucleic acids by ROS. Increase in oxidation of biomolecules leads to a state called as oxidative stress (OS). Finally, the increase in OS condition induces abnormality in physiology of animals under elevated temperature. Exposure of animals to rise in habitat temperature is found to boost the metabolism of animals and a very strong and positive correlation exists between metabolism and levels of ROS and OS. Continuous induction of OS is negatively correlated with survivability and longevity and positively correlated with ageing in animals. Thus, it can be predicted that continuous exposure of animals to acute or gradual rise in habitat temperature due to global warming may induce OS, reduced survivability and longevity in animals in general and poikilotherms in particular. A positive correlation between metabolism and temperature in general and altered O₂ consumption at elevated temperature in particular could also increase the risk of experiencing OS in homeotherms. Effects of global warming on longevity of animals through increased risk of protein misfolding and disease susceptibility due to OS as the cause or effects or both also cannot be ignored. Therefore, understanding the physiological impacts of global warming in relation to longevity of animals will become very crucial challenge to biologists of the present millennium.

• Reactive oxygen species
• Redox regulation
• Oxidative stress
• Survivability
• Climate change
• Disease susceptibility
• Global warming
• Longevity
• Thermal stress

• Compatible solute
• Daphnia magna
• Ectoine

• Amino Acids, Diamino/adverse effects
• Animals
• Catalase/metabolism
• Daphnia/drug effects
• Daphnia/physiology
• Heart Rate/drug effects
• Nitric Oxide/metabolism
• Survival Rate
• Swimming/physiology

• Acrylonitrile
• Acute toxicity
• Hazardous and Noxious Substances (HNS) spills
• Recovery period
• Seabass
• Sublethal toxicity

• Acrylonitrile/toxicity
• Animals
• Bass/physiology
• Catalase/metabolism
• Chemical Hazard Release
• Civil Defense
• Cytochrome P-450 CYP1A1/metabolism
• Glutathione Transferase/metabolism
• Hazardous Substances/toxicity
• Models, Biological
• Risk Assessment
• Superoxide Dismutase/metabolism
• Water Pollutants, Chemical/toxicity