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1
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Santa C, Park S, Gejt A, Clark HA, Hengerer B, Sergelen K. Real-time monitoring of vancomycin using a split-aptamer surface plasmon resonance biosensor. Analyst 2024; 150:131-141. [PMID: 39584594 DOI: 10.1039/d4an01226g] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/26/2024]
Abstract
Real-time monitoring of therapeutic drugs is crucial for treatment management and pharmacokinetic studies. We present the optimization and affinity tuning of split-aptamer sandwich assay for real-time monitoring of the narrow therapeutic window drug vancomycin, using surface plasmon resonance (SPR). To achieve reversible, label-free sensing of small molecules by SPR, we adapted a vancomycin binding aptamer in a sandwich assay format through the split-aptamer approach. By evaluating multiple split sites within the secondary structure of the original aptamer, we identified position 27 (P27) as optimal for preserving target affinity, ensuring reversibility, and maximizing sensitivity. The assay demonstrated robust performance under physiologically relevant ranges of pH and divalent cations, and the specific ternary complex formation of the aptamer split segments with the analyte was confirmed by circular dichroism spectroscopy. Subsequently, we engineered a series of split-aptamer pairs with increasing complementarity in the stem regions, improving both the affinity and limit of detection up to 10-fold, as compared to the primary P27 pair. The kinetics of the engineered split-aptamer pairs were evaluated, revealing fast association and dissociation rates, confirming improved affinity and detection limits across variants. Most importantly, the reversibility of the assay, essential for real-time monitoring, was maintained in all pairs. Finally, the assay was further validated in complex biological matrices, including the cerebrospinal fluid from dogs and diluted plasma from rats, demonstrating functionality in biological environments and stability exceeding 9 hours. Our study paves the way for applications of split-aptamers in real-time monitoring of small molecules, with potential implications for in vivo therapeutic drug monitoring and pharmacokinetic studies.
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Affiliation(s)
| | | | - Artur Gejt
- BioMed X Institute, Heidelberg, Germany.
- Faculty of Biotechnology, Mannheim University of Applied Sciences, Germany
| | - Heather A Clark
- School of Biological and Health Systems Engineering, Arizona State University, USA
| | - Bastian Hengerer
- Boehringer Ingelheim Pharma GmbH & Co. KG, CNS Research, Germany
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2
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Zhao Y, Gan Y, Chen J, Zheng H, Chang Y, Lin C. Recent reports on the sensing strategy and the On-site detection of illegal drugs. RSC Adv 2024; 14:6917-6929. [PMID: 38410368 PMCID: PMC10895702 DOI: 10.1039/d3ra06931a] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Grants] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 10/11/2023] [Accepted: 12/13/2023] [Indexed: 02/28/2024] Open
Abstract
In this review, works on the on-site detection of illegal drugs in recent years are summarised and discussed, most of which were published within the past five years. The detection methods are categorised as colourimetric, fluorescence, Raman spectrometry, ion mobility spectrometry, electrochemistry, and mass spectrometry. Also, strategies that are possibly suitable for on-site detection and the actual instrumentation to be used in the field are listed.
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Affiliation(s)
- Yang Zhao
- Key Laboratory of Drug Monitoring and Control, Drug Intelligence and Forensic Center, Ministry of Public Security P.R.C. No. 18 Dongbeiwang West Road, Haidian District 100193 Beijing China
- Institute of Forensic Science of the Ministry of Public Security No. 17 Muxidi Nanli, West City District 100038 Beijing China
| | - Yumeng Gan
- Institute for Biomimetics and Soft Matter, Fujian Provincial Key Laboratory for Soft Functional Materials Research, College of Physical Science and Technology, Xiamen University 9 Zengcuoan West Road 361005 Xiamen China
- State Key Laboratory of Physical Chemistry of Solid Surface Xiamen China
| | - Jun Chen
- Key Laboratory of Drug Monitoring and Control, Drug Intelligence and Forensic Center, Ministry of Public Security P.R.C. No. 18 Dongbeiwang West Road, Haidian District 100193 Beijing China
| | - Hui Zheng
- Key Laboratory of Drug Monitoring and Control, Drug Intelligence and Forensic Center, Ministry of Public Security P.R.C. No. 18 Dongbeiwang West Road, Haidian District 100193 Beijing China
| | - Ying Chang
- Institute of Forensic Science of the Ministry of Public Security No. 17 Muxidi Nanli, West City District 100038 Beijing China
| | - Changxu Lin
- Institute for Biomimetics and Soft Matter, Fujian Provincial Key Laboratory for Soft Functional Materials Research, College of Physical Science and Technology, Xiamen University 9 Zengcuoan West Road 361005 Xiamen China
- State Key Laboratory of Physical Chemistry of Solid Surface Xiamen China
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3
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Hariri AA, Cartwright AP, Dory C, Gidi Y, Yee S, Thompson IAP, Fu KX, Yang K, Wu D, Maganzini N, Feagin T, Young BE, Afshar BH, Eisenstein M, Digonnet MJF, Vuckovic J, Soh HT. Modular Aptamer Switches for the Continuous Optical Detection of Small-Molecule Analytes in Complex Media. ADVANCED MATERIALS (DEERFIELD BEACH, FLA.) 2024; 36:e2304410. [PMID: 37975267 DOI: 10.1002/adma.202304410] [Citation(s) in RCA: 6] [Impact Index Per Article: 6.0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Subscribe] [Scholar Register] [Received: 05/10/2023] [Revised: 11/10/2023] [Indexed: 11/19/2023]
Abstract
Aptamers are a promising class of affinity reagents because signal transduction mechanisms can be built into the reagent, so that they can directly produce a physically measurable output signal upon target binding. However, endowing the signal transduction functionality into an aptamer remains a trial-and-error process that can compromise its affinity or specificity and typically requires knowledge of the ligand binding domain or its structure. In this work, a design architecture that can convert an existing aptamer into a "reversible aptamer switch" whose kinetic and thermodynamic properties can be tuned without a priori knowledge of the ligand binding domain or its structure is described. Finally, by combining these aptamer switches with evanescent-field-based optical detection hardware that minimizes sample autofluorescence, this study demonstrates the first optical biosensor system that can continuously measure multiple biomarkers (dopamine and cortisol) in complex samples (artificial cerebrospinal fluid and undiluted plasma) with second and subsecond-scale time responses at physiologically relevant concentration ranges.
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Affiliation(s)
- Amani A Hariri
- Department of Radiology, Stanford University, Stanford, CA, 94305, USA
| | - Alyssa P Cartwright
- Department of Electrical Engineering, Stanford University, Stanford, CA, 94305, USA
| | - Constantin Dory
- Department of Electrical Engineering, Stanford University, Stanford, CA, 94305, USA
| | - Yasser Gidi
- Department of Radiology, Stanford University, Stanford, CA, 94305, USA
| | - Steven Yee
- Department of Electrical Engineering, Stanford University, Stanford, CA, 94305, USA
| | - Ian A P Thompson
- Department of Electrical Engineering, Stanford University, Stanford, CA, 94305, USA
| | - Kaiyu X Fu
- Department of Radiology, Stanford University, Stanford, CA, 94305, USA
- Department of Electrical Engineering, Stanford University, Stanford, CA, 94305, USA
| | - Kiyoul Yang
- Department of Electrical Engineering, Stanford University, Stanford, CA, 94305, USA
| | - Diana Wu
- Department of Bioengineering, Stanford University, Stanford, CA, 94305, USA
| | - Nicolò Maganzini
- Department of Electrical Engineering, Stanford University, Stanford, CA, 94305, USA
| | - Trevor Feagin
- Department of Radiology, Stanford University, Stanford, CA, 94305, USA
| | - Brian E Young
- Department of Radiology, Stanford University, Stanford, CA, 94305, USA
| | - Behrad Habib Afshar
- Department of Electrical Engineering, Stanford University, Stanford, CA, 94305, USA
| | | | - Michel J F Digonnet
- Department of Electrical Engineering, Stanford University, Stanford, CA, 94305, USA
| | - Jelena Vuckovic
- Department of Electrical Engineering, Stanford University, Stanford, CA, 94305, USA
| | - H Tom Soh
- Department of Radiology, Stanford University, Stanford, CA, 94305, USA
- Department of Electrical Engineering, Stanford University, Stanford, CA, 94305, USA
- Department of Bioengineering, Stanford University, Stanford, CA, 94305, USA
- Department of Chemical Engineering, Stanford University, Stanford, CA, 94305, USA
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4
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Mir TUG, Wani AK, Akhtar N, Katoch V, Shukla S, Kadam US, Hong JC. Advancing biological investigations using portable sensors for detection of sensitive samples. Heliyon 2023; 9:e22679. [PMID: 38089995 PMCID: PMC10711145 DOI: 10.1016/j.heliyon.2023.e22679] [Citation(s) in RCA: 3] [Impact Index Per Article: 1.5] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 05/23/2023] [Revised: 09/29/2023] [Accepted: 11/16/2023] [Indexed: 01/14/2024] Open
Abstract
Portable biosensors are emerged as powerful diagnostic tools for analyzing intricately complex biological samples. These biosensors offer sensitive detection capabilities by utilizing biomolecules such as proteins, nucleic acids, microbes or microbial products, antibodies, and enzymes. Their speed, accuracy, stability, specificity, and low cost make them indispensable in forensic investigations and criminal cases. Notably, portable biosensors have been developed to rapidly detect toxins, poisons, body fluids, and explosives; they have proven invaluable in forensic examinations of suspected samples, generating efficient results that enable effective and fair trials. One of the key advantages of portable biosensors is their ability to provide sensitive and non-destructive detection of forensic samples without requiring extensive sample preparation, thereby reducing the possibility of false results. This comprehensive review provides an overview of the current advancements in portable biosensors for the detection of sensitive materials, highlighting their significance in advancing investigations and enhancing sensitive sample detection capabilities.
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Affiliation(s)
- Tahir ul Gani Mir
- School of Bioengineering and Biosciences, Lovely Professional University, Phagwara, Punjab, 144411, India
- State Forensic Science Laboratory, Srinagar, Jammu and Kashmir, 190001, India
| | - Atif Khurshid Wani
- School of Bioengineering and Biosciences, Lovely Professional University, Phagwara, Punjab, 144411, India
| | - Nahid Akhtar
- School of Bioengineering and Biosciences, Lovely Professional University, Phagwara, Punjab, 144411, India
| | - Vaidehi Katoch
- School of Bioengineering and Biosciences, Lovely Professional University, Phagwara, Punjab, 144411, India
| | - Saurabh Shukla
- School of Bioengineering and Biosciences, Lovely Professional University, Phagwara, Punjab, 144411, India
| | - Ulhas Sopanrao Kadam
- Division of Life Science and Division of Applied Life Science (BK21 Four), Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Jinju, Gyeongnam, 52828, South Korea
| | - Jong Chan Hong
- Division of Life Science and Division of Applied Life Science (BK21 Four), Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Jinju, Gyeongnam, 52828, South Korea
- Division of Plant Sciences, University of Missouri, Columbia, MO, 65211, USA
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5
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Wang Z, Lou X. Recent Progress in Functional-Nucleic-Acid-Based Fluorescent Fiber-Optic Evanescent Wave Biosensors. BIOSENSORS 2023; 13:bios13040425. [PMID: 37185500 PMCID: PMC10135899 DOI: 10.3390/bios13040425] [Citation(s) in RCA: 2] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Subscribe] [Scholar Register] [Received: 02/16/2023] [Revised: 03/20/2023] [Accepted: 03/25/2023] [Indexed: 05/17/2023]
Abstract
Biosensors capable of onsite and continuous detection of environmental and food pollutants and biomarkers are highly desired, but only a few sensing platforms meet the "2-SAR" requirements (sensitivity, specificity, affordability, automation, rapidity, and reusability). A fiber optic evanescent wave (FOEW) sensor is an attractive type of portable device that has the advantages of high sensitivity, low cost, good reusability, and long-term stability. By utilizing functional nucleic acids (FNAs) such as aptamers, DNAzymes, and rational designed nucleic acid probes as specific recognition ligands, the FOEW sensor has been demonstrated to be a general sensing platform for the onsite and continuous detection of various targets ranging from small molecules and heavy metal ions to proteins, nucleic acids, and pathogens. In this review, we cover the progress of the fluorescent FNA-based FOEW biosensor since its first report in 1995. We focus on the chemical modification of the optical fiber and the sensing mechanisms for the five above-mentioned types of targets. The challenges and prospects on the isolation of high-quality aptamers, reagent-free detection, long-term stability under application conditions, and high throughput are also included in this review to highlight the future trends for the development of FOEW biosensors capable of onsite and continuous detection.
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Affiliation(s)
- Zheng Wang
- Department of Chemistry, Capital Normal University, Xisanhuan North Road. 105, Beijing 100048, China
| | - Xinhui Lou
- Department of Chemistry, Capital Normal University, Xisanhuan North Road. 105, Beijing 100048, China
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6
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Song Y, Zhang Y, Wang L, Hu C, Liu ZF, Feng XS, He ZW. Cocaine in Different Matrices: Recent Updates on Pretreatment and Detection Techniques. Crit Rev Anal Chem 2022; 54:529-548. [PMID: 35708993 DOI: 10.1080/10408347.2022.2087467] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 10/18/2022]
Abstract
Cocaine abuse has attracted increased attention in the recent past since it can cause addiction and great harm to the normal human body. Due to cocaine exists in various complex matrices, the detection of it in different matrices is helpful to prevent abuse. It is thus imperative to establish efficient and accurate methods for pretreatment and detection of cocaine in different samples. The present study provides a summary of the research progress of cocaine pretreatment methods (such as different microextraction methods, QuEChERS, and solid phase extraction based on novel extraction materials) and detection approaches (such as liquid chromatography coupled with different detectors, gas chromatography and related techniques, capillary electrophoresis and sensors). A comparison of the pros and cons of different pretreatment and detection methods is presented. The findings of this study will provide a reference for selection of the most suitable cocaine pretreatment and detection techniques.
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Affiliation(s)
- Yang Song
- School of Pharmacy, China Medical University, Shenyang, China
| | - Yuan Zhang
- School of Pharmacy, China Medical University, Shenyang, China
| | - Ling Wang
- Department of Pharmacy, Xiangya Hospital, Central South University, Changsha, China
| | - Cong Hu
- School of Pharmacy, China Medical University, Shenyang, China
| | - Zhi-Fei Liu
- School of Pharmacy, China Medical University, Shenyang, China
| | - Xue-Song Feng
- School of Pharmacy, China Medical University, Shenyang, China
| | - Zhen-Wei He
- Department of Neurology, The Fourth Affiliated Hospital of China Medical University, Shenyang, China
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7
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Moradi R, Khalili NP, Septiani NLW, Liu CH, Doustkhah E, Yamauchi Y, Rotkin SV. Nanoarchitectonics for Abused-Drug Biosensors. SMALL (WEINHEIM AN DER BERGSTRASSE, GERMANY) 2022; 18:e2104847. [PMID: 34882957 DOI: 10.1002/smll.202104847] [Citation(s) in RCA: 11] [Impact Index Per Article: 3.7] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 08/12/2021] [Revised: 10/14/2021] [Indexed: 06/13/2023]
Abstract
Rapid, accessible, and highly accurate biosensors for the detection of addictive and abused drugs are needed to reduce the adverse personal and societal impacts of addiction. Modern sensors that utilize next-generation technologies, e.g., nanobiotechnology and nanoarchitectonics, have triggered revolutionary progress in the field as they allow accurate detection and tracking of trace levels of major classes of drugs. This paper reviews advances in the field of biosensors for the detection of commonly abused drugs, both prescribed such as codeine and morphine, and illegal narcotics like cocaine.
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Affiliation(s)
- Rasoul Moradi
- Nanotechnology Laboratory, School of Engineering and Applied Science, Khazar University, Baku, Az1096, Azerbaijan
- Department of Chemical Engineering, School of Engineering and Applied Science, Khazar University, Baku, Az1096, Azerbaijan
| | - Nazila Pour Khalili
- Nanotechnology Laboratory, School of Engineering and Applied Science, Khazar University, Baku, Az1096, Azerbaijan
- Center for Cell Pathology Research, Department of Biological Science, Khazar University, Baku, Az1096, Azerbaijan
| | - Ni Luh Wulan Septiani
- Advanced Functional Materials Research Group, Institut Teknologi Bandung, Bandung, 40132, Indonesia
| | - Chia-Hung Liu
- Department of Urology, School of Medicine, College of Medicine, and TMU Research Center of Urology and Kidney, Taipei Medical University, No. 250, Wu-Hsing Street, Taipei, 110, Taiwan
- Department of Urology, Shuang Ho Hospital, Taipei Medical University, No. 291, Zhongzheng Road, Zhonghe District, New Taipei City, 23561, Taiwan
| | - Esmail Doustkhah
- International Center for Materials Nanoarchitechtonics (WPI-MANA), National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki, 305-0044, Japan
- JST-ERATO Yamauchi Materials Space-Tectonics Project and International Center for Materials Nanoarchitectonics (WPI-MANA), National Institute for Materials Science (NIMS), 1-1 Namiki, Tsukuba, Ibaraki, 305-0044, Japan
| | - Yusuke Yamauchi
- JST-ERATO Yamauchi Materials Space-Tectonics Project and International Center for Materials Nanoarchitectonics (WPI-MANA), National Institute for Materials Science (NIMS), 1-1 Namiki, Tsukuba, Ibaraki, 305-0044, Japan
- School of Chemical Engineering and Australian Institute for Bioengineering and Nanotechnology (AIBN), The University of Queensland, Brisbane, QLD, 4072, Australia
| | - Slava V Rotkin
- Department of Engineering Science and Mechanics, Materials Research Institute, The Pennsylvania State University, Millennium Science Complex, University Park, PA, 16802, USA
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8
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Lei Z, Guo B. 2D Material-Based Optical Biosensor: Status and Prospect. ADVANCED SCIENCE (WEINHEIM, BADEN-WURTTEMBERG, GERMANY) 2022; 9:e2102924. [PMID: 34898053 PMCID: PMC8811838 DOI: 10.1002/advs.202102924] [Citation(s) in RCA: 61] [Impact Index Per Article: 20.3] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Subscribe] [Scholar Register] [Received: 07/08/2021] [Revised: 09/05/2021] [Indexed: 05/07/2023]
Abstract
The combination of 2D materials and optical biosensors has become a hot research topic in recent years. Graphene, transition metal dichalcogenides, black phosphorus, MXenes, and other 2D materials (metal oxides and degenerate semiconductors) have unique optical properties and play a unique role in the detection of different biomolecules. Through the modification of 2D materials, optical biosensor has the advantages that traditional sensors (such as electrical sensing) do not have, and the sensitivity and detection limit are greatly improved. Here, optical biosensors based on different 2D materials are reviewed. First, various detection methods of biomolecules, including surface plasmon resonance (SPR), fluorescence resonance energy transfer (FRET), and evanescent wave and properties, preparation and integration strategies of 2D material, are introduced in detail. Second, various biosensors based on 2D materials are summarized. Furthermore, the applications of these optical biosensors in biological imaging, food safety, pollution prevention/control, and biological medicine are discussed. Finally, the future development of optical biosensors is prospected. It is believed that with their in-depth research in the laboratory, optical biosensors will gradually become commercialized and improve people's quality of life in many aspects.
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Affiliation(s)
- Zong‐Lin Lei
- Key Lab of In‐Fiber Integrated Optics of Ministry of Education of ChinaHarbin Engineering UniversityHarbin150001China
| | - Bo Guo
- Key Lab of In‐Fiber Integrated Optics of Ministry of Education of ChinaHarbin Engineering UniversityHarbin150001China
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9
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Loyez M, DeRosa MC, Caucheteur C, Wattiez R. Overview and emerging trends in optical fiber aptasensing. Biosens Bioelectron 2022; 196:113694. [PMID: 34637994 DOI: 10.1016/j.bios.2021.113694] [Citation(s) in RCA: 17] [Impact Index Per Article: 5.7] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/10/2021] [Revised: 09/30/2021] [Accepted: 10/01/2021] [Indexed: 12/16/2022]
Abstract
Optical fiber biosensors have attracted growing interest over the last decade and quickly became a key enabling technology, especially for the detection of biomarkers at extremely low concentrations and in small volumes. Among the many and recent fiber-optic sensing amenities, aptamers-based sensors have shown unequalled performances in terms of ease of production, specificity, and sensitivity. The immobilization of small and highly stable bioreceptors such as DNA has bolstered their use for the most varied applications e.g., medical diagnosis, food safety and environmental monitoring. This review highlights the recent advances in aptamer-based optical fiber biosensors. An in-depth analysis of the literature summarizes different fiber-optic structures and biochemical strategies for molecular detection and immobilization of receptors over diverse surfaces. In this review, we analyze the features offered by those sensors and discuss about the next challenges to be addressed. This overview investigates both biochemical and optical parameters, drawing the guiding lines for forthcoming innovations and prospects in this ever-growing field of research.
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Affiliation(s)
- Médéric Loyez
- Proteomics and Microbiology Department, University of Mons, Avenue du Champ de Mars 6, 7000, Mons, Belgium; Electromagnetism and Telecommunication Department, University of Mons, Bld. Dolez 31, 7000, Mons, Belgium.
| | - Maria C DeRosa
- Department of Chemistry, 203 Steacie Building, Carleton University, 1125, Colonel By Drive, Ottawa, ON K1S 5B6, Canada
| | - Christophe Caucheteur
- Electromagnetism and Telecommunication Department, University of Mons, Bld. Dolez 31, 7000, Mons, Belgium
| | - Ruddy Wattiez
- Proteomics and Microbiology Department, University of Mons, Avenue du Champ de Mars 6, 7000, Mons, Belgium
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10
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Rapid and quantitative detection of SARS-CoV-2 IgG antibody in serum using optofluidic point-of-care testing fluorescence biosensor. Talanta 2021; 235:122800. [PMID: 34517658 PMCID: PMC8363510 DOI: 10.1016/j.talanta.2021.122800] [Citation(s) in RCA: 19] [Impact Index Per Article: 4.8] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 04/21/2021] [Revised: 07/23/2021] [Accepted: 08/11/2021] [Indexed: 12/25/2022]
Abstract
The COVID-19 pandemic brings unprecedented crisis for public health and economics in the world. Detecting specific antibodies to SARS-CoV-2 is a powerful supplement for the diagnosis of COVID-19 and is important for epidemiological studies and vaccine validations. Herein, a rapid and quantitative detection method of anti-SARS-CoV-2 IgG antibody was built based on the optofluidic point-of-care testing fluorescence biosensor. Without complicated steps needed, the portable system is suitable for on-site sensitive determination of anti-SARS-CoV-2 IgG antibody in serum. Under the optimal conditions, the whole detection procedure is about 25 min with a detection limit of 12.5 ng/mL that can well meet the diagnostic requirements. The method was not obviously affected by IgM and serum matrix and demonstrated to have good stability and reliability in real sample analysis. Compared to ELISA test results, the proposed method exhibits several advantages including wider measurement range and easier operation. The method provides a universal platform for rapid and quantitative analysis of other related biomarkers, which is of significance for the prevention and control of COVID-19 pandemic.
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11
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Lim S, Kuang Y, Ardoña HAM. Evolution of Supramolecular Systems Towards Next-Generation Biosensors. Front Chem 2021; 9:723111. [PMID: 34490210 PMCID: PMC8416679 DOI: 10.3389/fchem.2021.723111] [Citation(s) in RCA: 6] [Impact Index Per Article: 1.5] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 06/10/2021] [Accepted: 08/09/2021] [Indexed: 11/17/2022] Open
Abstract
Supramolecular materials, which rely on dynamic non-covalent interactions, present a promising approach to advance the capabilities of currently available biosensors. The weak interactions between supramolecular monomers allow for adaptivity and responsiveness of supramolecular or self-assembling systems to external stimuli. In many cases, these characteristics improve the performance of recognition units, reporters, or signal transducers of biosensors. The facile methods for preparing supramolecular materials also allow for straightforward ways to combine them with other functional materials and create multicomponent sensors. To date, biosensors with supramolecular components are capable of not only detecting target analytes based on known ligand affinity or specific host-guest interactions, but can also be used for more complex structural detection such as chiral sensing. In this Review, we discuss the advancements in the area of biosensors, with a particular highlight on the designs of supramolecular materials employed in analytical applications over the years. We will first describe how different types of supramolecular components are currently used as recognition or reporter units for biosensors. The working mechanisms of detection and signal transduction by supramolecular systems will be presented, as well as the important hierarchical characteristics from the monomers to assemblies that contribute to selectivity and sensitivity. We will then examine how supramolecular materials are currently integrated in different types of biosensing platforms. Emerging trends and perspectives will be outlined, specifically for exploring new design and platforms that may bring supramolecular sensors a step closer towards practical use for multiplexed or differential sensing, higher throughput operations, real-time monitoring, reporting of biological function, as well as for environmental studies.
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Affiliation(s)
- Sujeung Lim
- Department of Chemical and Biomolecular Engineering, Samueli School of Engineering, University of California, Irvine, Irvine, CA, United States
| | - Yuyao Kuang
- Department of Chemical and Biomolecular Engineering, Samueli School of Engineering, University of California, Irvine, Irvine, CA, United States
| | - Herdeline Ann M Ardoña
- Department of Chemical and Biomolecular Engineering, Samueli School of Engineering, University of California, Irvine, Irvine, CA, United States.,Department of Biomedical Engineering, Samueli School of Engineering, University of California, Irvine, Irvine, CA, United States.,Department of Chemistry, School of Physical Sciences, University of California, Irvine, Irvine, CA, United States.,Sue & Bill Gross Stem Cell Research Center, University of California, Irvine, Irvine, CA, United States
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12
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Yue F, Li F, Kong Q, Guo Y, Sun X. Recent advances in aptamer-based sensors for aminoglycoside antibiotics detection and their applications. THE SCIENCE OF THE TOTAL ENVIRONMENT 2021; 762:143129. [PMID: 33121792 DOI: 10.1016/j.scitotenv.2020.143129] [Citation(s) in RCA: 35] [Impact Index Per Article: 8.8] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 08/15/2020] [Revised: 10/13/2020] [Accepted: 10/14/2020] [Indexed: 05/25/2023]
Abstract
Aminoglycoside antibiotics (AAs) have been extensively applied in medical field and animal husbandry owing to desirable broad-spectrum antibacterial activity. Excessive AAs residues in the environment can be accumulated in human body through food chain and cause detrimental effect on human health. The establishment of highly specific, simple and sensitive detection methods for monitoring AAs residues is highly in demand. Aptasensor using aptamer as the biological recognition element is the efficient and promising sensing method for detection of AAs. In this review, we have made a summary of specific aptamers sequences against AAs. Subsequently, we provide a systematical and comprehensive overview of modern techniques in aptasensors for detection of AAs according to optical aptasensors as well as electrochemical aptasensors and further summarize their advantages and disadvantages to compare their applications. In addition, we present an overview of practical applications of aptasensors in sample detection of AAs. Moreover, the current challenges and future trends in this field are also included to reveal a promising perspective for developing novel aptasensors for AAs.
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Affiliation(s)
- Fengling Yue
- School of Agricultural Engineering and Food Science, Shandong University of Technology, No.12 Zhangzhou Road, Zibo 255049, Shandong Province, China; Shandong Provincial Engineering Research Center of Vegetable Safety and Quality Traceability, No. 12 Zhangzhou Road, Zibo 255049, Shandong Province, China; Zibo City Key Laboratory of Agricultural Product Safety Traceability, China
| | - Falan Li
- School of Agricultural Engineering and Food Science, Shandong University of Technology, No.12 Zhangzhou Road, Zibo 255049, Shandong Province, China; Shandong Provincial Engineering Research Center of Vegetable Safety and Quality Traceability, No. 12 Zhangzhou Road, Zibo 255049, Shandong Province, China; Zibo City Key Laboratory of Agricultural Product Safety Traceability, China
| | - Qianqian Kong
- School of Agricultural Engineering and Food Science, Shandong University of Technology, No.12 Zhangzhou Road, Zibo 255049, Shandong Province, China; Shandong Provincial Engineering Research Center of Vegetable Safety and Quality Traceability, No. 12 Zhangzhou Road, Zibo 255049, Shandong Province, China; Zibo City Key Laboratory of Agricultural Product Safety Traceability, China
| | - Yemin Guo
- School of Agricultural Engineering and Food Science, Shandong University of Technology, No.12 Zhangzhou Road, Zibo 255049, Shandong Province, China; Shandong Provincial Engineering Research Center of Vegetable Safety and Quality Traceability, No. 12 Zhangzhou Road, Zibo 255049, Shandong Province, China; Zibo City Key Laboratory of Agricultural Product Safety Traceability, China
| | - Xia Sun
- School of Agricultural Engineering and Food Science, Shandong University of Technology, No.12 Zhangzhou Road, Zibo 255049, Shandong Province, China; Shandong Provincial Engineering Research Center of Vegetable Safety and Quality Traceability, No. 12 Zhangzhou Road, Zibo 255049, Shandong Province, China; Zibo City Key Laboratory of Agricultural Product Safety Traceability, China.
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13
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Debiais M, Lelievre A, Smietana M, Müller S. Splitting aptamers and nucleic acid enzymes for the development of advanced biosensors. Nucleic Acids Res 2020; 48:3400-3422. [PMID: 32112111 PMCID: PMC7144939 DOI: 10.1093/nar/gkaa132] [Citation(s) in RCA: 100] [Impact Index Per Article: 20.0] [Reference Citation Analysis] [Abstract] [MESH Headings] [Grants] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 12/02/2019] [Revised: 02/12/2020] [Accepted: 02/18/2020] [Indexed: 12/16/2022] Open
Abstract
In analogy to split-protein systems, which rely on the appropriate fragmentation of protein domains, split aptamers made of two or more short nucleic acid strands have emerged as novel tools in biosensor set-ups. The concept relies on dissecting an aptamer into a series of two or more independent fragments, able to assemble in the presence of a specific target. The stability of the assembled structure can further be enhanced by functionalities that upon folding would lead to covalent end-joining of the fragments. To date, only a few aptamers have been split successfully, and application of split aptamers in biosensing approaches remains as promising as it is challenging. Further improving the stability of split aptamer target complexes and with that the sensitivity as well as efficient working modes are important tasks. Here we review functional nucleic acid assemblies that are derived from aptamers and ribozymes/DNAzymes. We focus on the thrombin, the adenosine/ATP and the cocaine split aptamers as the three most studied DNA split systems and on split DNAzyme assemblies. Furthermore, we extend the subject into split light up RNA aptamers used as mimics of the green fluorescent protein (GFP), and split ribozymes.
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Affiliation(s)
- Mégane Debiais
- Institut des Biomolécules Max Mousseron, University of Montpellier, CNRS, ENCSM, Montpellier, France
| | - Amandine Lelievre
- University Greifswald, Institute for Biochemistry, Greifswald, Germany
| | - Michael Smietana
- Institut des Biomolécules Max Mousseron, University of Montpellier, CNRS, ENCSM, Montpellier, France
| | - Sabine Müller
- University Greifswald, Institute for Biochemistry, Greifswald, Germany
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14
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Pirzada M, Altintas Z. Recent Progress in Optical Sensors for Biomedical Diagnostics. MICROMACHINES 2020; 11:E356. [PMID: 32235546 PMCID: PMC7231100 DOI: 10.3390/mi11040356] [Citation(s) in RCA: 52] [Impact Index Per Article: 10.4] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 03/01/2020] [Revised: 03/25/2020] [Accepted: 03/28/2020] [Indexed: 12/12/2022]
Abstract
In recent years, several types of optical sensors have been probed for their aptitude in healthcare biosensing, making their applications in biomedical diagnostics a rapidly evolving subject. Optical sensors show versatility amongst different receptor types and even permit the integration of different detection mechanisms. Such conjugated sensing platforms facilitate the exploitation of their neoteric synergistic characteristics for sensor fabrication. This paper covers nearly 250 research articles since 2016 representing the emerging interest in rapid, reproducible and ultrasensitive assays in clinical analysis. Therefore, we present an elaborate review of biomedical diagnostics with the help of optical sensors working on varied principles such as surface plasmon resonance, localised surface plasmon resonance, evanescent wave fluorescence, bioluminescence and several others. These sensors are capable of investigating toxins, proteins, pathogens, disease biomarkers and whole cells in varied sensing media ranging from water to buffer to more complex environments such as serum, blood or urine. Hence, the recent trends discussed in this review hold enormous potential for the widespread use of optical sensors in early-stage disease prediction and point-of-care testing devices.
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Affiliation(s)
| | - Zeynep Altintas
- Institute of Chemistry, Technical University of Berlin, Straße des 17. Juni 124, 10623 Berlin, Germany;
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15
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Gao L, Deng Z, Lin Y, Sulemana H, Shi H, Yu C, Chen S. Highly sensitive detection for cocaine using an aptamer-modified molybdenum disulfide/gold nanoparticle microarray. NEW J CHEM 2020. [DOI: 10.1039/d0nj02342f] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/21/2022]
Abstract
The detection of cocaine based on an aptamer-modified molybdenum disulfide@gold nanoparticle (MoS2@AuNP) nanosheet array immobilized on aminated slides was achieved.
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Affiliation(s)
- Li Gao
- Department of Otolaryngology Head and Neck Surgery
- Zhujiang Hospital
- Southern Medical University
- Guangzhou
- China
| | - Zebin Deng
- Institute of Life Sciences
- Jiangsu University
- Zhenjiang 212013
- P. R. China
| | - Yuanwei Lin
- Institute of Life Sciences
- Jiangsu University
- Zhenjiang 212013
- P. R. China
| | - Husseini Sulemana
- Institute of Life Sciences
- Jiangsu University
- Zhenjiang 212013
- P. R. China
| | - Haixia Shi
- Institute of Life Sciences
- Jiangsu University
- Zhenjiang 212013
- P. R. China
| | - Chaosheng Yu
- Department of Otolaryngology Head and Neck Surgery
- Zhujiang Hospital
- Southern Medical University
- Guangzhou
- China
| | - Shuaijun Chen
- Department of Otolaryngology Head and Neck Surgery
- Zhujiang Hospital
- Southern Medical University
- Guangzhou
- China
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16
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Gao L, Wang H, Deng Z, Xiang W, Shi H, Xie B, Shi H. Highly sensitive detection for cocaine using an aptamer–cocaine–aptamer method. NEW J CHEM 2020. [DOI: 10.1039/c9nj05147c] [Citation(s) in RCA: 6] [Impact Index Per Article: 1.2] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/15/2022]
Abstract
In this study, a thiol-modified aptamer was immobilized on a gold nanoparticle-modified molybdenum disulfide composite material (MoS2@AuNPs), which could remove the interference of physical adsorption and reduce false positive signals.
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Affiliation(s)
- Li Gao
- Institute of Life Sciences
- Jiangsu University
- Zhenjiang 212013
- P. R. China
| | - Huixing Wang
- Institute of Life Sciences
- Jiangsu University
- Zhenjiang 212013
- P. R. China
| | - Zebin Deng
- Institute of Life Sciences
- Jiangsu University
- Zhenjiang 212013
- P. R. China
| | - Wenwen Xiang
- Institute of Life Sciences
- Jiangsu University
- Zhenjiang 212013
- P. R. China
| | - Haifeng Shi
- Institute of Life Sciences
- Jiangsu University
- Zhenjiang 212013
- P. R. China
| | - Bing Xie
- Department of Obstetrics and Gynecology
- the Fourth People's Hospital of Zhenjiang
- Zhenjiang
- P. R. China
| | - Haixia Shi
- P. E. Department of Jiangsu University
- Zhenjiang 212013
- P. R. China
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17
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Affiliation(s)
- Xu-dong Wang
- Department of Chemistry, Fudan University, 200433 Shanghai, P. R. China
| | - Otto S. Wolfbeis
- Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, D-93040 Regensburg, Germany
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18
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Celikbas E, Balaban S, Evran S, Coskunol H, Timur S. A Bottom-Up Approach for Developing Aptasensors for Abused Drugs: Biosensors in Forensics. BIOSENSORS 2019; 9:E118. [PMID: 31581533 PMCID: PMC6955935 DOI: 10.3390/bios9040118] [Citation(s) in RCA: 13] [Impact Index Per Article: 2.2] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 09/15/2019] [Revised: 09/27/2019] [Accepted: 09/30/2019] [Indexed: 02/07/2023]
Abstract
Aptamer-based point-of-care (POC) diagnostics platforms may be of substantial benefit in forensic analysis as they provide rapid, sensitive, user-friendly, and selective analysis tools for detection. Aptasensors have not yet been adapted commercially. However, the significance of the applications of aptasensors in the literature exceeded their potential. Herein, in this review, a bottom-up approach is followed to describe the aptasensor development and application procedure, starting from the synthesis of the corresponding aptamer sequence for the selected analyte to creating a smart surface for the sensitive detection of the molecule of interest. Optical and electrochemical biosensing platforms, which are designed with aptamers as recognition molecules, detecting abused drugs are critically reviewed, and existing and possible applications of different designs are discussed. Several potential disciplines in which aptamer-based biosensing technology can be of greatest value, including forensic drug analysis and biological evidence, are then highlighted to encourage researchers to focus on developing aptasensors in these specific areas.
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Affiliation(s)
- Eda Celikbas
- Department of Biochemistry, Institute of Natural and Applied Sciences, Ege University, 35100 Bornova, Izmir, Turkey;
| | - Simge Balaban
- Department of Biochemistry, Institute of Natural and Applied Sciences, Ege University, 35100 Bornova, Izmir, Turkey;
| | - Serap Evran
- Department of Biochemistry, Institute of Natural and Applied Sciences, Ege University, 35100 Bornova, Izmir, Turkey;
- Department of Biochemistry, Faculty of Science, Ege University, 35100 Bornova, Izmir, Turkey
| | - Hakan Coskunol
- Department of Mental Health and Diseases, Faculty of Medicine, Ege University, 35100 Bornova, Izmir, Turkey;
| | - Suna Timur
- Department of Biochemistry, Institute of Natural and Applied Sciences, Ege University, 35100 Bornova, Izmir, Turkey;
- Department of Biochemistry, Faculty of Science, Ege University, 35100 Bornova, Izmir, Turkey
- Central Research Testing and Analysis Laboratory Research and Application Center, Ege University, 35100 Bornova, Izmir, Turkey
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19
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Celebanska A, Chiniforooshan Y, Janik M, Mikulic P, Sellamuthu B, Walsh R, Perreault J, Bock WJ. Label-free cocaine aptasensor based on a long-period fiber grating. OPTICS LETTERS 2019; 44:2482-2485. [PMID: 31090712 DOI: 10.1364/ol.44.002482] [Citation(s) in RCA: 4] [Impact Index Per Article: 0.7] [Reference Citation Analysis] [Abstract] [Track Full Text] [Subscribe] [Scholar Register] [Received: 02/08/2019] [Accepted: 04/16/2019] [Indexed: 06/09/2023]
Abstract
In this Letter, we combined a promising bioreceptor, a cocaine aptamer MN6, with an ultrasensitive optical platform long-period fiber grating (LPFG) to create a new cocaine biosensor. The cocaine induces a conformational rearrangement of the aptamer which changes the refractive index around the LPFG producing a measurable shift of the transmission spectrum. We were able to track subtle interaction between the receptor and cocaine molecules over a concentration range of 25 to 100 μM. The presented biosensor does not require labeling or signal enhancement, resulting in a simple user-friendly device.
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20
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A novel dual-color total internal reflection fluorescence detecting platform using compact optical structure and silicon-based photodetector. Talanta 2018; 196:78-84. [PMID: 30683414 DOI: 10.1016/j.talanta.2018.12.039] [Citation(s) in RCA: 8] [Impact Index Per Article: 1.1] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 07/27/2018] [Revised: 12/01/2018] [Accepted: 12/12/2018] [Indexed: 11/24/2022]
Abstract
A novel dual-color total internal reflection fluorescence detecting platform (DTP) was developed for the simultaneous detection of two fluorescence dyes. The DTP employed a simple and compact optical system and a Si-based photodetector SOP-1000 assembly to improve the optical efficiency and detection sensitivity. In the optical system, a single-multimode fiber optic coupler was applied for the transmission of two wavelength excitation lights and the collection and transmission of two wavelength fluorescence signals, and a fiber optical switch controlled two wavelength excited lights to alternatively enter into the combined tapered fiber optic probe and two wavelength fluorescence dyes were alternatively excited. The simultaneous and sensitive detection of two wavelength fluorescence signals was achieved by one photodetector SOP-1000 based on the time-resolved effect of the fiber optical switch. The DTP demonstrated its sensitivity of 50 fW light intensity, and the limit of detection of Cy5.5 and Pacific Blue dye (PB) were 0.05 nM and 2.1 nM, respectively. This miniaturized and integrated DTP with high sensitivity, simple optical structure, negligible cross-talk, and cost-effectiveness could be a potential alternative to the conventional dual-color fluorescence detecting systems. It could also be a powerful component of a μ-TAS system for highly sensitive dual-color fluorescence detection.
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21
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Sergelen K, Liedberg B, Knoll W, Dostálek J. A surface plasmon field-enhanced fluorescence reversible split aptamer biosensor. Analyst 2018; 142:2995-3001. [PMID: 28744534 DOI: 10.1039/c7an00970d] [Citation(s) in RCA: 16] [Impact Index Per Article: 2.3] [Reference Citation Analysis] [Abstract] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 01/07/2023]
Abstract
Surface plasmon field-enhanced fluorescence is reported for the readout of a heterogeneous assay that utilizes low affinity split aptamer ligands. Weak affinity ligands that reversibly interact with target analytes hold potential for facile implementation in continuous monitoring biosensor systems. This functionality is not possible without the regeneration of more commonly used assays relying on high affinity ligands and end-point measurement. In fluorescence-based sensors, the use of low affinity ligands allows avoiding this step but it imposes a challenge associated with the weak optical response to the specific capture of the target analyte which is also often masked by a strong background. The coupling of fluorophore labels with a confined field of surface plasmons is reported for strong amplification of the fluorescence signal emitted from the sensor surface and its efficient discrimination from the background. This optical scheme is demonstrated for time-resolved analysis of chosen model analytes - adenoside and adenosine triphosphate - with a split aptamer that exhibits an equilibrium affinity binding constant between 0.73 and 1.35 mM. The developed biosensor enables rapid and specific discrimination of target analyte concentration changes from low μM to mM in buffer as well as in 10% serum.
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Affiliation(s)
- K Sergelen
- BioSensor Technologies, AIT-Austrian Institute of Technology, Muthgasse 11, 1190 Vienna, Austria.
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22
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Qiu Y, Tang Y, Li B, He M. Rapid detection of cocaine using aptamer-based biosensor on an evanescent wave fibre platform. ROYAL SOCIETY OPEN SCIENCE 2018; 5:180821. [PMID: 30473831 PMCID: PMC6227954 DOI: 10.1098/rsos.180821] [Citation(s) in RCA: 14] [Impact Index Per Article: 2.0] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Subscribe] [Scholar Register] [Received: 07/26/2018] [Accepted: 09/13/2018] [Indexed: 05/11/2023]
Abstract
The rapid detection of cocaine has received considerable attention because of the instantaneous and adverse effects of cocaine overdose on human health. Aptamer-based biosensors for cocaine detection have been well established for research and application. However, reducing the analytic duration without deteriorating the sensitivity still remains as a challenge. Here, we proposed an aptamer-based evanescent wave fibre (EWF) biosensor to rapidly detect cocaine in a wide working range. At first, the aptamers were conjugated to complementary DNA with fluorescence tag and such conjugants were then immobilized on magnetic beads. After cocaine was introduced to compete against the aptamer-DNA conjugants, the released DNA in supernatant was detected on the EWF platform. The dynamic curves of EWF signals could be interpreted by the first-order kinetics and saturation model. The semi-log calibration curve covered a working range of 10-5000 µM of cocaine, and the limit of detection was approximately 10.5 µM. The duration of the full procedure was 990 s (16.5 min), and the detection interval was 390 s (6.5 min). The specified detection of cocaine was confirmed from four typical pharmaceutic agents. The analysis was repeated for 50 cycles without significant loss of sensitivity. Therefore, the aptamer-based EWF biosensor is a feasible solution to rapidly detect cocaine.
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Affiliation(s)
- Yong Qiu
- State Key Joint Laboratory of Environment Simulation and Pollution Control, School of Environment, Tsinghua University, Beijing 100084, People's Republic of China
| | - Yunfei Tang
- State Key Joint Laboratory of Environment Simulation and Pollution Control, School of Environment, Tsinghua University, Beijing 100084, People's Republic of China
- Ecological Environmental Protection Investments Company, China Communications Construction Corporation, Beijing 100013, People's Republic of China
| | - Bing Li
- School of Energy and Environmental Engineering, University of Beijing Science and Technology, Beijing 100083, People's Republic of China
| | - Miao He
- State Key Joint Laboratory of Environment Simulation and Pollution Control, School of Environment, Tsinghua University, Beijing 100084, People's Republic of China
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23
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Li W, Wang H, Yang R, Song D, Long F, Zhu A. Integrated multichannel all-fiber optofluidic biosensing platform for sensitive and simultaneous detection of trace analytes. Anal Chim Acta 2018; 1040:112-119. [PMID: 30327100 DOI: 10.1016/j.aca.2018.07.067] [Citation(s) in RCA: 8] [Impact Index Per Article: 1.1] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 05/08/2018] [Revised: 07/21/2018] [Accepted: 07/26/2018] [Indexed: 02/06/2023]
Abstract
An integrated multichannel all-fiber optofluidic biosensing platform (M-AOB) has been developed for a sensitive, rapid, and simultaneous detection of up to three trace analytes. The M-AOB platform employs a 1 × 3 fiber optical switch and three single-multimode fiber optic couplers for the transmission of excitation light and fluorescence and one photodiode detector for the simultaneous detection of fluorescence signals of multiple channels based on the time-resolve effect of the fiber optical switch. This design greatly simplified the entire system structure and improved light transmission efficiency. Through an indirect competitive immunoassay mechanism, we detected two highly regulated small molecules, namely, atrazine and 2,4-D, to demonstrate the value of M-AOB to the simultaneous measurement of trace analytes in water samples. The limits of detection of 0.03 μg/L and 0.04 μg/L were obtained for atrazine and 2,4-D, respectively, and were highly comparable with those of other analytical techniques. The high sensitivity of M-AOB benefited from the high light collective efficiency and low light loss of the excellent all-fiber optical structures and from the advantages of the evanescent wave technique. The regeneration of the biosensor surface, 200 assay cycles, were performed without any significant activity loss. Each assay cycle was less than 15 min. The immunoassay performance of the M-AOB, evaluated in several spiked water samples, showed good recovery, accuracy, and precision, indicating that the M-AOB was less susceptible to matrix effects of water samples. All these results illustrated that M-AOB can be readily extended toward the simultaneous and rapid detection of other trace small molecules using different biosensors modified by other analyte conjugates and their respective fluorescence-labeled antibodies.
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Affiliation(s)
- Wei Li
- School of Environment and Natural Resources, Renmin University of China, Beijing, 100872, China
| | - Haoyu Wang
- School of Environment and Natural Resources, Renmin University of China, Beijing, 100872, China
| | - Rong Yang
- School of Environment and Natural Resources, Renmin University of China, Beijing, 100872, China
| | - Dan Song
- School of Environment and Natural Resources, Renmin University of China, Beijing, 100872, China
| | - Feng Long
- School of Environment and Natural Resources, Renmin University of China, Beijing, 100872, China.
| | - Anna Zhu
- Research Institute of Chemical Defense, Academy of Military Sciences PLA China, Beijing, 102205, China; State Key Laboratory of NBC Protection FOR Civilian, Beijing, 102205, China.
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24
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Garrido E, Pla L, Lozano‐Torres B, El Sayed S, Martínez‐Máñez R, Sancenón F. Chromogenic and Fluorogenic Probes for the Detection of Illicit Drugs. ChemistryOpen 2018; 7:401-428. [PMID: 29872615 PMCID: PMC5974560 DOI: 10.1002/open.201800034] [Citation(s) in RCA: 16] [Impact Index Per Article: 2.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/07/2018] [Indexed: 01/02/2023] Open
Abstract
The consumption of illicit drugs has increased exponentially in recent years and has become a problem that worries both governments and international institutions. The rapid emergence of new compounds, their easy access, the low levels at which these substances are able to produce an effect, and their short time of permanence in the organism make it necessary to develop highly rapid, easy, sensitive, and selective methods for their detection. Currently, the most widely used methods for drug detection are based on techniques that require large measurement times, the use of sophisticated equipment, and qualified personnel. Chromo- and fluorogenic methods are an alternative to those classical procedures.
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Affiliation(s)
- Eva Garrido
- Instituto Interuniversitario de Investigación de Reconocimiento Molecular y, Desarrollo Tecnológico (IDM)Universitat Politècnica de València, Universitat de ValènciaCamí de Vera s/n46022ValènciaSpain
| | - Luis Pla
- Instituto Interuniversitario de Investigación de Reconocimiento Molecular y, Desarrollo Tecnológico (IDM)Universitat Politècnica de València, Universitat de ValènciaCamí de Vera s/n46022ValènciaSpain
| | - Beatriz Lozano‐Torres
- Instituto Interuniversitario de Investigación de Reconocimiento Molecular y, Desarrollo Tecnológico (IDM)Universitat Politècnica de València, Universitat de ValènciaCamí de Vera s/n46022ValènciaSpain
| | - Sameh El Sayed
- Instituto Interuniversitario de Investigación de Reconocimiento Molecular y, Desarrollo Tecnológico (IDM)Universitat Politècnica de València, Universitat de ValènciaCamí de Vera s/n46022ValènciaSpain
- CIBER de BioingenieríaBiomateriales y Nanomedicina (CIBER-BBN)
| | - Ramón Martínez‐Máñez
- Instituto Interuniversitario de Investigación de Reconocimiento Molecular y, Desarrollo Tecnológico (IDM)Universitat Politècnica de València, Universitat de ValènciaCamí de Vera s/n46022ValènciaSpain
- CIBER de BioingenieríaBiomateriales y Nanomedicina (CIBER-BBN)
- Departmento de QuímicaUniversitat Politècnica de ValènciaCamí de Vera s/n46022ValènciaSpain
| | - Félix Sancenón
- Instituto Interuniversitario de Investigación de Reconocimiento Molecular y, Desarrollo Tecnológico (IDM)Universitat Politècnica de València, Universitat de ValènciaCamí de Vera s/n46022ValènciaSpain
- CIBER de BioingenieríaBiomateriales y Nanomedicina (CIBER-BBN)
- Departmento de QuímicaUniversitat Politècnica de ValènciaCamí de Vera s/n46022ValènciaSpain
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25
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Rossetti M, Porchetta A. Allosterically regulated DNA-based switches: From design to bioanalytical applications. Anal Chim Acta 2018; 1012:30-41. [PMID: 29475471 DOI: 10.1016/j.aca.2017.12.046] [Citation(s) in RCA: 25] [Impact Index Per Article: 3.6] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/06/2017] [Revised: 12/10/2017] [Accepted: 12/28/2017] [Indexed: 02/07/2023]
Abstract
DNA-based switches are structure-switching biomolecules widely employed in different bioanalytical applications. Of particular interest are DNA-based switches whose activity is regulated through the use of allostery. Allostery is a naturally occurring mechanism in which ligand binding induces the modulation and fine control of a connected biomolecule function as a consequence of changes in concentration of the effector. Through this general mechanism, many different allosteric DNA-based switches able to respond in a highly controlled way at the presence of a specific molecular effector have been engineered. Here, we discuss how to design allosterically regulated DNA-based switches and their applications in the field of molecular sensing, diagnostic and drug release.
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Affiliation(s)
- Marianna Rossetti
- Chemistry Department, University of Rome, Tor Vergata, Via della Ricerca Scientifica, 00133, Rome, Italy
| | - Alessandro Porchetta
- Chemistry Department, University of Rome, Tor Vergata, Via della Ricerca Scientifica, 00133, Rome, Italy.
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26
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Zhang J, Ran F, Zhou W, Shang B, Yu F, Wu L, Hu W, He X, Chen Q. Ultrasensitive fluorescent aptasensor for MUC1 detection based on deoxyribonuclease I-aided target recycling signal amplification. RSC Adv 2018; 8:32009-32015. [PMID: 35547495 PMCID: PMC9085725 DOI: 10.1039/c8ra06498a] [Citation(s) in RCA: 11] [Impact Index Per Article: 1.6] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/02/2018] [Accepted: 09/09/2018] [Indexed: 01/02/2023] Open
Abstract
A novel sensing strategy for sensitive detection of mucin 1 protein (MUC1) based on deoxyribonuclease I-aided target recycling signal amplification was proposed. In this paper, in the absence of MUC1, the MUC1 aptamer is absorbed on the surface of graphene oxide (GO) via π-stacking interactions. This results in quenching of the fluorescent label and no fluorescence signal is observed. Upon adding MUC1, the probe sequences could be specifically recognized by MUC1, leading to an increase in the fluorescence intensity. The detection limit is as low as 10 pg mL−1, and a linear range from 50 pg mL−1 to 100 ng mL−1. The assay is specific and sensitive, and successfully applied to the determination of MUC1 in spiked human serum, urine and saliva. Importantly, the proposed aptasensing strategy has great potential in detecting various protein and even cancer cells. A novel sensing strategy for sensitive detection of mucin 1 protein (MUC1) based on deoxyribonuclease I-aided target recycling signal amplification was proposed.![]()
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Affiliation(s)
- Jun Zhang
- Affiliated Dongfeng Hospital
- Hubei University of Medicine
- Shiyan
- China
| | - Fengying Ran
- Affiliated Dongfeng Hospital
- Hubei University of Medicine
- Shiyan
- China
| | - Wenbo Zhou
- Affiliated Dongfeng Hospital
- Hubei University of Medicine
- Shiyan
- China
| | - Bing Shang
- Affiliated Dongfeng Hospital
- Hubei University of Medicine
- Shiyan
- China
| | - Fei Yu
- Affiliated Dongfeng Hospital
- Hubei University of Medicine
- Shiyan
- China
| | - Lun Wu
- Affiliated Dongfeng Hospital
- Hubei University of Medicine
- Shiyan
- China
| | - Wanbao Hu
- Sinopharm Dongfeng Huaguo Hospital
- Shiyan 442008
- China
| | - Xueqin He
- Affiliated Dongfeng Hospital
- Hubei University of Medicine
- Shiyan
- China
| | - Qinhua Chen
- Affiliated Dongfeng Hospital
- Hubei University of Medicine
- Shiyan
- China
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27
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Evanescent wave aptasensor for continuous and online aminoglycoside antibiotics detection based on target binding facilitated fluorescence quenching. Biosens Bioelectron 2017; 102:646-651. [PMID: 29268187 DOI: 10.1016/j.bios.2017.12.006] [Citation(s) in RCA: 50] [Impact Index Per Article: 6.3] [Reference Citation Analysis] [Abstract] [Key Words] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/15/2017] [Revised: 11/22/2017] [Accepted: 12/05/2017] [Indexed: 10/18/2022]
Abstract
The biosensors capable for on-site continuous and online monitoring of pollutants in environment are highly desired due to their practical importance and convenience. The group specific detection of pollutants is especially attractive due to the diversity of environmental pollutants. Here we devise an evanescent wave aptasensor based on target binding facilitated fluorescence quenching (FQ-EWA) for the online continuous and group-specific detection of aminoglycoside antibiotics (AMGAs). In FQ-EWA, a fluorophore labeled DNA aptamer selected against kanamycin was used for both the target recognition in solution and signal transduction on optical fiber of EWA. The aptamers form multiple-strand complex (M-Apt) in the absence of AMGAs. The binding between AMGA and the aptamer disrupts M-Apt and leads to the formation of AMGA -aptamer complex (AMGA-Apt). The photo-induced electron transfer between the fluorophore and AMGA partially quenches the fluorescence of AMGA-Apt. The structure-selective absorption of AMGA-Apt over M-Apt on the graphene oxide further quenches the fluorescence of AMGA-Apt. Meanwhile, the unbound aptamers in solution assemble with the unlabeled aptamers immobilized on the fiber to form M-Apt. The amount of M-Apt on the fiber is inversely proportional to the concentration of AMGAs, enabling the signal-off detection of AMGAs from 200nM to 200μM with a detection limit of 26nM. The whole detection process is carried out in an online mode without any offline operation, providing a great benefit for system automation and miniaturization. FQ-EWA also shows great surface regeneration capability and enables the continuous detection more than 60 times.
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Fluorometric aptamer based determination of adenosine triphosphate based on deoxyribonuclease I-aided target recycling and signal amplification using graphene oxide as a quencher. Mikrochim Acta 2017. [DOI: 10.1007/s00604-017-2194-3] [Citation(s) in RCA: 23] [Impact Index Per Article: 2.9] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 02/06/2023]
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Febbraio F. Biochemical strategies for the detection and detoxification of toxic chemicals in the environment. World J Biol Chem 2017; 8:13-20. [PMID: 28289515 PMCID: PMC5329710 DOI: 10.4331/wjbc.v8.i1.13] [Citation(s) in RCA: 10] [Impact Index Per Article: 1.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 08/28/2016] [Revised: 12/12/2016] [Accepted: 01/18/2017] [Indexed: 02/05/2023] Open
Abstract
Addressing the problems related to the widespread presence of an increasing number of chemicals released into the environment by human activities represents one of the most important challenges of this century. In the last few years, to replace the high cost, in terms of time and money, of conventional technologies, the scientific community has directed considerable research towards the development both of new detection systems for the measurement of the contamination levels of chemicals in people’s body fluids and tissue, as well as in the environment, and of new remediation strategies for the removal of such chemicals from the environment, as a means of the prevention of human diseases. New emerging biosensors for the analysis of environmental chemicals have been proposed, including VHH antibodies, that combine the antibody performance with the affinity for small molecules, genetically engineered microorganisms, aptamers and new highly stable enzymes. However, the advances in the field of chemicals monitoring are still far from producing a continuous real-time and on-line system for their detection. Better results have been obtained in the development of strategies which use organisms (microorganisms, plants and animals) or metabolic pathway-based approaches (single enzymes or more complex enzymatic solutions) for the fixation, degradation and detoxification of chemicals in the environment. Systems for enzymatic detoxification and degradation of toxic agents in wastewater from chemical and manufacturing industries, such as ligninolytic enzymes for the treatment of wastewater from the textile industry, have been proposed. Considering the high value of these research studies, in terms of the protection of human health and of the ecosystem, science must play a major role in guiding policy changes in this field.
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Li GZ, Tang D. Bioresponsive controlled glucose release from TiO2 nanotube arrays: a simple and portable biosensing system for cocaine with a glucometer readout. J Mater Chem B 2017. [DOI: 10.1039/c7tb00670e] [Citation(s) in RCA: 9] [Impact Index Per Article: 1.1] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/17/2022]
Abstract
Herein, we design a simple and portable biosensing platform for the quantitative detection of cocaine based on target-triggered glucose release from a TiO2 nanotube array (TiNTA) with a glucometer readout.
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Affiliation(s)
- Guang-Zhou Li
- Department of Sports and Health
- Chongqing Three Gorges University
- Wanzhou 404100
- China
- Key Laboratory of Analysis and Detection for Food Safety (MOE & Fujian Province)
| | - Dianping Tang
- Key Laboratory of Analysis and Detection for Food Safety (MOE & Fujian Province)
- Collaborative Innovation Center of Detection Technology for Haixi Food Safety and Products (Fujian Province)
- State Key Laboratory of Photocatalysis on Energy and Environment
- Department of Chemistry
- Fuzhou University
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