Intervention effects and related mechanisms of glycyrrhizic acid on zebrafish with Hirschsprung-associated enterocolitis

Table 1 Primer sequence of gene of interest

Gene	Primer sequence (5’-3’)	Length
ef1α	F: CTTCTCAGGCTGACTGTGC	358 bp
	R: CCGCTAGCATTACCCTCC
IL-1β	F: CCAGCTCTGAAATGATGGCAT	139 bp
	R: TCGCATCTGTAGCTCATTGC
IL-8	F: ATTGAAACAGAAAGCCGA	150 bp
	R: CTTAACCCATGGAGCAGA

IL: Interleukin.

Table 2 Proportion of two phenotypes in zebrafish of all groups

Group	Normal phenotype	Intestinal lumen enlargement
Blank control group	0/50	0/50
Model control group	11/45	34/45
DMSO + 1.25 μg/mL GA group	13/43	30/43
DMSO + 12.5 μg/mL GA group	12/46	34/46
DMSO + 50 μg/mL GA group	18/32	14/32

DMSO: Dimethyl sulfoxide; GA: Glycyrrhizic acid.