Basic Study
Copyright ©The Author(s) 2023. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastrointest Surg. Jul 27, 2023; 15(7): 1317-1330
Published online Jul 27, 2023. doi: 10.4240/wjgs.v15.i7.1317
Intervention effects and related mechanisms of glycyrrhizic acid on zebrafish with Hirschsprung-associated enterocolitis
Ming-Kun Liu, Ying-Jian Chen, Fei Chen, Zhi-Xiong Lin, Zi-Cheng Zhu, Yu Lin, Yi-Fan Fang, Dian-Ming Wu
Ming-Kun Liu, Fei Chen, Yu Lin, Yi-Fan Fang, Dian-Ming Wu, Department of Pediatric Surgery, Fujian Maternity and Child Health Hospital, College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics, Fujian Medical University, Fuzhou 350001, Fujian Province, China
Ming-Kun Liu, Fei Chen, Yu Lin, Yi-Fan Fang, Dian-Ming Wu, Department of Pediatric Surgery, Fujian Children’s Hospital (Fujian Branch of Shanghai Children’s Medical Center), College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics, Fujian Medical University, Fuzhou 350000, Fujian Province, China
Ying-Jian Chen, Zhi-Xiong Lin, Department of Pediatric Surgery, Fujian Children’s Hospital, Fuzhou 350001, Fujian Province, China
Zi-Cheng Zhu, Department of Clinical Medicine, Fujian Medical University, Fuzhou 350001, Fujian Province, China
Author contributions: Liu MK was responsible for data collection and analysis and wrote the paper; Liu MK and Chen YJ contributed equally to this article; Chen F and Lin Y contributed to the data analysis; Lin ZX and Zhu ZC completed the basic experiment; Wu DM and Fang YF designed the research and provided clinical advice; and all authors read and approved the final manuscript.
Supported by Joint Funds for the Innovation of Science and Technology, Fujian Province, No. 2020Y9139; and Startup Fund for Scientific Research, Fujian Medical University, No. 2019QH1141.
Institutional animal care and use committee statement: All animal experiments conformed to the principles for the care and use of laboratory animals. The experimental scheme was approved by the Medical Ethics Committee of Fujian Maternity and Child Health Hospital.
Conflict-of-interest statement: All the authors report no relevant conflicts of interest for this article.
Data sharing statement: The experimental data related to this study can be obtained from the corresponding author upon request.
ARRIVE guidelines statement: The authors have read the ARRIVE guidelines, and the manuscript was prepared and revised according to the ARRIVE guidelines.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Dian-Ming Wu, BM BCh, Chief Physician, Department of Pediatric Surgery, Fujian Maternity and Child Health Hospital, College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics, Fujian Medical University, No. 18 Daoshan Road, Gulou District, Fuzhou 350001, Fujian Province, China. wdm2369@126.com
Received: March 14, 2023
Peer-review started: March 14, 2023
First decision: April 7, 2023
Revised: April 24, 2023
Accepted: May 11, 2023
Article in press: May 11, 2023
Published online: July 27, 2023
Core Tip

Core Tip: As an important late-stage inflammatory mediator, high mobility group box 1 protein (HMGB1) is involved in the occurrence, development and outcome of many chronic inflammatory diseases and autoimmune diseases. Glycyrrhizic acid (GA) is the most widely used HMGB1 inhibitor, which can directly bind to HMGB1 and inhibit its chemotactic and mitotic activity. Here, we constructed a zebrafish Hirschsprung-associated enterocolitis (HAEC) model and used different concentrations of GA for intervention. Through the intervention study, we understood the effects of HMGB1 inhibitors-GA on the phenotype and inflammatory state of HAEC zebrafish, and the study results provided a new reference for the prevention and treatment of HAEC.